(Circular) (Six-base) MAPSORT of: pFN21AE5753.seq Check: 7755 from: 1 to: 6880 pFN21AE5753 6880 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 6880 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 4223 1937 Size: 214 2072 4594 Fragments arranged by size: 4594 2072 214 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3233 3848 4943 6327 6595 1632 Size: 1601 615 1095 1384 268 1917 Fragments arranged by size: 1917 1601 1384 1095 615 268 AflII C'TTAA_G Cuts at: 820 1017 3089 820 Size: 197 2072 4611 Fragments arranged by size: 4611 2072 197 AflIII A'CryG_T Cuts at: 1203 3143 5779 6756 1203 Size: 1940 2636 977 1327 Fragments arranged by size: 2636 1940 1327 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 2203 1166 Size: 1037 5843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6880 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2328 2360 4000 4032 2328 Size: 32 1640 32 5176 Fragments arranged by size: 5176 1640 32 32 AlwNI CAG_nnn'CTG Cuts at: 3323 3568 3649 3992 4623 6195 3323 Size: 245 81 343 631 1572 4008 Fragments arranged by size: 4008 1572 631 343 245 81 ApaLI G'TGCA_C Cuts at: 4937 6093 4937 Size: 1156 5724 ApoI r'AATT_y Cuts at: 1493 2218 2443 3982 4189 4415 4567 6676 1493 Size: 725 225 1539 207 226 152 2109 1697 Fragments arranged by size: 2109 1697 1539 725 226 225 207 152 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 4205 1870 Size: 73 2262 4545 Fragments arranged by size: 4545 2262 73 AvrII C'CTAG_G Cuts at: 2425 3104 2425 Size: 679 6201 BamHI G'GATC_C Cuts at: 2359 4210 4577 2359 Size: 1851 367 4662 Fragments arranged by size: 4662 1851 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3100 3300 3474 3754 4201 Size: 325 217 627 1313 200 174 280 447 Cuts at: 4201 5656 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1313 627 447 325 280 217 200 174 BanII G_rGCy'C Cuts at: 1391 3609 4199 1391 Size: 2218 590 4072 Fragments arranged by size: 4072 2218 590 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3239 3947 928 Size: 971 1340 708 3861 Fragments arranged by size: 3861 1340 971 708 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5071 5105 1903 Size: 34 3134 34 3678 Fragments arranged by size: 3678 3134 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 3283 4772 5982 2002 Size: 1281 1489 1210 2900 Fragments arranged by size: 2900 1489 1281 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2562 3318 5491 Size: 107 122 71 631 605 890 756 2173 Cuts at: 5491 136 Size: 1525 Fragments arranged by size: 2173 1525 890 756 631 605 122 107 71 BglII A'GATC_T Cuts at: 2117 6875 2117 Size: 4758 2122 BlpI GC'TnA_GC Cuts at: 2454 2667 4297 2454 Size: 213 1630 5037 Fragments arranged by size: 5037 1630 213 Bme1580I G_kGCm'C Cuts at: 1790 3103 3477 3757 4941 6097 1790 Size: 1313 374 280 1184 1156 2573 Fragments arranged by size: 2573 1313 1184 1156 374 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 4611 5569 Size: 416 53 83 399 24 689 2505 958 Cuts at: 5569 442 Size: 1753 Fragments arranged by size: 2505 1753 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 6880 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2064 2858 3718 3789 3907 4072 5540 1239 Size: 825 794 860 71 118 165 1468 2579 Fragments arranged by size: 2579 1468 860 825 794 165 118 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2316 3243 5005 5870 6168 2316 Size: 927 1762 865 298 3028 Fragments arranged by size: 3028 1762 927 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2609 3206 3374 3410 3761 5543 882 Size: 617 1110 597 168 36 351 1782 2219 Fragments arranged by size: 2219 1782 1110 617 597 351 168 36 BsaAI yAC'GTr Cuts at: 493 1204 3144 4671 493 Size: 711 1940 1527 2702 Fragments arranged by size: 2702 1940 1527 711 BsaBI GATnn'nnATC Cuts at: 6874 6874 Size: 6880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4687 5069 5704 275 Size: 53 83 186 936 3154 382 635 1451 Fragments arranged by size: 3154 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5313 5711 5985 6132 6543 5313 Size: 398 274 147 411 5650 Fragments arranged by size: 5650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3218 3248 1178 Size: 30 165 30 1815 30 4810 Fragments arranged by size: 4810 1815 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 3355 3481 3521 1186 Size: 1441 728 126 40 4545 Fragments arranged by size: 4545 1441 728 126 40 BseYI C'CCAG_C Cuts at: 2459 2636 6083 6579 2459 Size: 177 3447 496 2760 Fragments arranged by size: 3447 2760 496 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2558 1163 Size: 573 822 5485 Fragments arranged by size: 5485 822 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4941 5026 6097 4941 Size: 85 1071 5724 Fragments arranged by size: 5724 1071 85 BsmI GAATG_Cn' Cuts at: 2171 3439 4391 4484 2171 Size: 1268 952 93 4567 Fragments arranged by size: 4567 1268 952 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3103 3477 3609 3757 4199 4941 5026 Size: 399 1313 374 132 148 442 742 85 Cuts at: 5026 6097 1391 Size: 1071 2174 Fragments arranged by size: 2174 1313 1071 742 442 399 374 148 132 85 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 3130 4769 6499 3130 Size: 1639 1730 3511 Fragments arranged by size: 3511 1730 1639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 2489 4235 844 Size: 1209 436 1746 3489 Fragments arranged by size: 3489 1746 1209 436 BsrBI CCG'CTC Cuts at: 1394 1823 4767 1394 Size: 429 2944 3507 Fragments arranged by size: 3507 2944 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2325 5375 5549 65 Size: 1183 1077 3050 174 1396 Fragments arranged by size: 3050 1396 1183 1077 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5524 5711 1700 Size: 110 3714 187 2869 Fragments arranged by size: 3714 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6880 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 4940 5952 4940 Size: 1012 5868 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6880 BstBI TT'CG_AA Cuts at: 5684 5684 Size: 6880 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6880 BstXI CCAn_nnnn'nTGG Cuts at: 2862 3365 3782 2862 Size: 503 417 5960 Fragments arranged by size: 5960 503 417 BstYI r'GATC_y Cuts at: 1972 2117 2359 3007 3988 4210 4577 4962 4979 Size: 145 242 648 981 222 367 385 17 Cuts at: 4979 6431 6517 6529 6875 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 981 648 385 367 346 242 222 145 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3968 3968 Size: 6880 BtgI C'CryG_G Cuts at: 513 1065 2004 2813 3594 513 Size: 552 939 809 781 3799 Fragments arranged by size: 3799 939 809 781 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3713 487 Size: 683 56 2487 3654 Fragments arranged by size: 3654 2487 683 56 BtsI GCAGTG_nn' Cuts at: 2614 3831 4396 5190 5210 2614 Size: 1217 565 794 20 4284 Fragments arranged by size: 4284 1217 794 565 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6845 DraI TTT'AAA Cuts at: 2592 4184 2592 Size: 1592 5288 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5887 809 Size: 507 4571 1802 Fragments arranged by size: 4571 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2810 4327 5216 Size: 54 1425 50 275 18 980 1517 889 Cuts at: 5216 6845 8 Size: 1629 43 Fragments arranged by size: 1629 1517 1425 980 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2314 2761 2862 3499 4018 4132 4810 1353 Size: 961 447 101 637 519 114 678 3423 Fragments arranged by size: 3423 961 678 637 519 447 114 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5467 5841 5987 387 Size: 1064 138 3878 374 146 1280 Fragments arranged by size: 3878 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2858 3233 3718 3789 3848 3907 Size: 393 432 794 375 485 71 59 59 Cuts at: 3907 4072 4943 5540 6327 6595 1239 Size: 165 871 597 787 268 1524 Fragments arranged by size: 1524 871 794 787 597 485 432 393 375 268 165 71 59 59 EcoNI CCTnn'n_nnAGG Cuts at: 2404 3108 3996 2404 Size: 704 888 5288 Fragments arranged by size: 5288 888 704 EcoO109I rG'GnC_Cy Cuts at: 3859 4002 3859 Size: 143 6737 EcoRI G'AATT_C Cuts at: 2218 3982 4189 4567 2218 Size: 1764 207 378 4531 Fragments arranged by size: 4531 1764 378 207 FspI TGC'GCA Cuts at: 5386 5386 Size: 6880 HaeII r_GCGC'y Cuts at: 1678 6027 1678 Size: 4349 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2963 2984 2995 3016 4131 Size: 32 135 32 1391 21 11 21 1115 Cuts at: 4131 4163 5521 5553 5595 5627 1373 Size: 32 1358 32 42 32 2626 Fragments arranged by size: 2626 1391 1358 1115 135 42 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 4224 4470 669 Size: 988 281 2286 246 3079 Fragments arranged by size: 3079 2286 988 281 246 HindIII A'AGCT_T Cuts at: 748 2129 748 Size: 1381 5499 HpaI GTT'AAC Cuts at: 4470 4470 Size: 6880 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5994 6178 1305 Size: 4689 184 2007 Fragments arranged by size: 4689 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2812 10 Size: 54 1425 343 980 4078 Fragments arranged by size: 4078 1425 980 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2203 3144 4838 5197 5356 518 Size: 648 1037 941 1694 359 159 2042 Fragments arranged by size: 2042 1694 1037 941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2341 4296 4973 6121 6366 1606 Size: 735 1955 677 1148 245 2120 Fragments arranged by size: 2120 1955 1148 735 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2813 3594 513 Size: 552 939 809 781 3799 Fragments arranged by size: 3799 939 809 781 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6880 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NspI r_CATG'y Cuts at: 1132 1229 4238 5783 1132 Size: 97 3009 1545 2229 Fragments arranged by size: 3009 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6880 PciI A'CATG_T Cuts at: 5779 5779 Size: 6880 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6880 PfoI T'CCnGG_A Cuts at: 3884 3884 Size: 6880 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4002 4002 Size: 6880 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3412 3444 3412 Size: 32 6848 PstI C_TGCA'G Cuts at: 830 3328 4167 4232 830 Size: 2498 839 65 3478 Fragments arranged by size: 3478 2498 839 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 2341 2341 Size: 6880 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SanDI GG'GwC_CC Cuts at: 4002 4002 Size: 6880 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 3022 5128 6840 3022 Size: 2106 1712 3062 Fragments arranged by size: 3062 2106 1712 SfcI C'TryA_G Cuts at: 826 1045 2431 2620 3324 4163 4228 5363 6044 Size: 219 1386 189 704 839 65 1135 681 Cuts at: 6044 6235 826 Size: 191 1471 Fragments arranged by size: 1471 1386 1135 839 704 681 219 191 189 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 4207 1872 Size: 2335 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2331 3089 3258 4984 5885 6147 Size: 197 926 388 758 169 1726 901 262 Cuts at: 6147 820 Size: 1553 Fragments arranged by size: 1726 1553 926 901 758 388 262 197 169 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SspI AAT'ATT Cuts at: 5 52 4804 5 Size: 47 4752 2081 Fragments arranged by size: 4752 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6880 StyI C'CwwG_G Cuts at: 513 1065 2004 2425 2560 2813 3104 3594 3679 Size: 552 939 421 135 253 291 490 85 Cuts at: 3679 4319 513 Size: 640 3074 Fragments arranged by size: 3074 939 640 552 490 421 291 253 135 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3046 3318 3524 4907 4924 5077 5262 Size: 1255 1466 272 206 1383 17 153 185 Cuts at: 5262 5707 325 Size: 445 1498 Fragments arranged by size: 1498 1466 1383 1255 445 272 206 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2708 3020 3690 Size: 274 80 33 51 1443 731 312 670 Cuts at: 3690 5126 6838 96 Size: 1436 1712 138 Fragments arranged by size: 1712 1443 1436 731 670 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2674 2706 3467 3499 3866 3898 6840 6872 2674 Size: 32 761 32 367 32 2942 32 2682 Fragments arranged by size: 2942 2682 761 367 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6880 XbaI T'CTAG_A Cuts at: 4216 4216 Size: 6880 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4047 4047 Size: 6880 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6880 XmaI C'CCGG_G Cuts at: 1870 4205 1870 Size: 2335 4545 XmnI GAAnn'nnTTC Cuts at: 5009 5009 Size: 6880 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI