(Linear) (Six-base) MAPSORT of: KIEE5753.seq Check: 8533 from: 1 to: 2175 KIEE5753.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 48 2175 Size: 48 2127 AccI GT'mk_AC Cuts at: 0 146 2175 Size: 146 2029 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1228 1843 2175 Size: 1228 615 332 Fragments arranged by size: 1228 615 332 AflII C'TTAA_G Cuts at: 0 1084 2175 Size: 1084 1091 AflIII A'CryG_T Cuts at: 0 1138 2175 Size: 1138 1037 AleI CACnn'nnGTG Cuts at: 0 198 2175 Size: 198 1977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 323 355 1995 2027 2175 Size: 323 32 1640 32 148 Fragments arranged by size: 1640 323 148 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 1318 1563 1644 1987 2175 Size: 1318 245 81 343 188 Fragments arranged by size: 1318 343 245 188 81 ApoI r'AATT_y Cuts at: 0 213 438 1977 2175 Size: 213 225 1539 198 Fragments arranged by size: 1539 225 213 198 AvrII C'CTAG_G Cuts at: 0 420 1099 2175 Size: 420 679 1076 Fragments arranged by size: 1076 679 420 BamHI G'GATC_C Cuts at: 0 354 2175 Size: 354 1821 BanI G'GyrC_C Cuts at: 0 1095 1295 1469 1749 2175 Size: 1095 200 174 280 426 Fragments arranged by size: 1095 426 280 200 174 BanII G_rGCy'C Cuts at: 0 1604 2175 Size: 1604 571 BbsI GAAGACnn'nnnn_ Cuts at: 0 1234 1942 2175 Size: 1234 708 233 Fragments arranged by size: 1234 708 233 BciVI GTATCCnnnnn_n' Cuts at: 0 1278 2175 Size: 1278 897 BglI GCCn_nnn'nGGC Cuts at: 0 557 1313 2175 Size: 557 756 862 Fragments arranged by size: 862 756 557 BglII A'GATC_T Cuts at: 0 112 2175 Size: 112 2063 BlpI GC'TnA_GC Cuts at: 0 449 662 2175 Size: 449 213 1513 Fragments arranged by size: 1513 449 213 Bme1580I G_kGCm'C Cuts at: 0 1098 1472 1752 2175 Size: 1098 374 280 423 Fragments arranged by size: 1098 423 374 280 BmrI ACTGGGnnnn_n' Cuts at: 0 101 2175 Size: 101 2074 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 78 2175 Size: 78 2097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 59 853 1713 1784 1902 2067 2175 Size: 59 794 860 71 118 165 108 Fragments arranged by size: 860 794 165 118 108 71 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1238 2175 Size: 311 927 937 Fragments arranged by size: 937 927 311 BsaI GGTCTCn'nnnn_ Cuts at: 0 604 1201 1369 1405 1756 2175 Size: 604 597 168 36 351 419 Fragments arranged by size: 604 597 419 351 168 36 BsaAI yAC'GTr Cuts at: 0 1139 2175 Size: 1139 1036 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1213 1243 2175 Size: 1213 30 932 Fragments arranged by size: 1213 932 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 622 1350 1476 1516 2175 Size: 622 728 126 40 659 Fragments arranged by size: 728 659 622 126 40 BseYI C'CCAG_C Cuts at: 0 454 631 2175 Size: 454 177 1544 Fragments arranged by size: 1544 454 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 2175 Size: 553 1622 BsmI GAATG_Cn' Cuts at: 0 166 1434 2175 Size: 166 1268 741 Fragments arranged by size: 1268 741 166 Bsp1286I G_dGCh'C Cuts at: 0 1098 1472 1604 1752 2175 Size: 1098 374 132 148 423 Fragments arranged by size: 1098 423 374 148 132 BspHI T'CATG_A Cuts at: 0 1125 2175 Size: 1125 1050 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 484 2175 Size: 48 436 1691 Fragments arranged by size: 1691 436 48 BsrDI GCAATG_nn' Cuts at: 0 320 2175 Size: 320 1855 BstXI CCAn_nnnn'nTGG Cuts at: 0 857 1360 1777 2175 Size: 857 503 417 398 Fragments arranged by size: 857 503 417 398 BstYI r'GATC_y Cuts at: 0 112 354 1002 1983 2175 Size: 112 242 648 981 192 Fragments arranged by size: 981 648 242 192 112 Bsu36I CC'TnA_GG Cuts at: 0 1963 2175 Size: 1963 212 BtgI C'CryG_G Cuts at: 0 808 1589 2175 Size: 808 781 586 Fragments arranged by size: 808 781 586 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1708 2175 Size: 1708 467 BtsI GCAGTG_nn' Cuts at: 0 609 1826 2175 Size: 609 1217 349 Fragments arranged by size: 1217 609 349 DraI TTT'AAA Cuts at: 0 587 2175 Size: 587 1588 EaeI y'GGCC_r Cuts at: 0 805 2175 Size: 805 1370 EarI CTCTTCn'nnn_ Cuts at: 0 309 756 857 1494 2013 2127 2175 Size: 309 447 101 637 519 114 48 Fragments arranged by size: 637 519 447 309 114 101 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 59 853 1228 1713 1784 1843 1902 2067 Size: 59 794 375 485 71 59 59 165 Cuts at: 2067 2175 Size: 108 Fragments arranged by size: 794 485 375 165 108 71 59 59 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 399 1103 1991 2175 Size: 399 704 888 184 Fragments arranged by size: 888 704 399 184 EcoO109I rG'GnC_Cy Cuts at: 0 1854 1997 2175 Size: 1854 143 178 Fragments arranged by size: 1854 178 143 EcoRI G'AATT_C Cuts at: 0 213 1977 2175 Size: 213 1764 198 Fragments arranged by size: 1764 213 198 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 958 979 990 1011 2126 2158 2175 Size: 958 21 11 21 1115 32 17 Fragments arranged by size: 1115 958 32 21 21 17 11 HindIII A'AGCT_T Cuts at: 0 124 2175 Size: 124 2051 MscI TGG'CCA Cuts at: 0 807 2175 Size: 807 1368 MslI CAynn'nnrTG Cuts at: 0 198 1139 2175 Size: 198 941 1036 Fragments arranged by size: 1036 941 198 MspA1I CmG'CkG Cuts at: 0 336 2175 Size: 336 1839 NcoI C'CATG_G Cuts at: 0 808 1589 2175 Size: 808 781 586 Fragments arranged by size: 808 781 586 PfoI T'CCnGG_A Cuts at: 0 1879 2175 Size: 1879 296 PpuMI rG'GwC_Cy Cuts at: 0 1997 2175 Size: 1997 178 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1407 1439 2175 Size: 1407 32 736 Fragments arranged by size: 1407 736 32 PstI C_TGCA'G Cuts at: 0 1323 2162 2175 Size: 1323 839 13 Fragments arranged by size: 1323 839 13 PvuII CAG'CTG Cuts at: 0 336 2175 Size: 336 1839 SanDI GG'GwC_CC Cuts at: 0 1997 2175 Size: 1997 178 ScaI AGT'ACT Cuts at: 0 1017 2175 Size: 1017 1158 SfcI C'TryA_G Cuts at: 0 426 615 1319 2158 2175 Size: 426 189 704 839 17 Fragments arranged by size: 839 704 426 189 17 SmlI C'TyrA_G Cuts at: 0 326 1084 1253 2175 Size: 326 758 169 922 Fragments arranged by size: 922 758 326 169 StyI C'CwwG_G Cuts at: 0 420 555 808 1099 1589 1674 2175 Size: 420 135 253 291 490 85 501 Fragments arranged by size: 501 490 420 291 253 135 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1041 1313 1519 2175 Size: 1041 272 206 656 Fragments arranged by size: 1041 656 272 206 TatI w'GTAC_w Cuts at: 0 703 1015 1685 2175 Size: 703 312 670 490 Fragments arranged by size: 703 670 490 312 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 669 701 1462 1494 1861 1893 2175 Size: 669 32 761 32 367 32 282 Fragments arranged by size: 761 669 367 282 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2042 2175 Size: 2042 133 Enzymes that do cut: AarI AccI AcuI AflII AflIII AleI AloI AlwNI ApoI AvrII BamHI BanI BanII BbsI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MscI MslI MspA1I NcoI PfoI PpuMI PsrI PstI PvuII SanDI ScaI SfcI SmlI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI BbvCI BcgI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI