################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 21 GGTACC G^GTACC AhdI 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 802 GATCC GGATC(4/5) Asp700I 446 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 21 GGTACC G^GTACC AspEI 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 21 GGTACC G^G[CT][GA]CC BbsI 334 GAAGAC GAAGAC(2/6) BpmI 31 CTGGAG CTGGAG(16/14) BpuAI 334 GAAGAC GAAGAC(2/6) BsgI 824 CTGCAC GTGCAG(16/14) BsmFI 881 GGGAC GGGAC(10/14) BsmI 766 GAATGC GAATGC(1/-1) Bsp1407I 118 TGTACA T^GTACA BsrGI 118 TGTACA T^GTACA BstAPI 51 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 443 TTCGAA TT^CGAA BstNI 457 CCAGG CC^[AT]GG BstYI 801 AGATCC [GA]^GATC[CT] DpnII 802 GATC ^GATC DraI 567 TTTAAA TTT^AAA DrdI 247 GACCAGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 457 CCAGG ^CC[AT]GG EcoRV 358 GATATC GAT^ATC EcoT14I 635 CCAAGG C^C[AT][AT]GG KpnI 21 GGTACC GGTAC^C MboI 802 GATC ^GATC MflI 801 AGATCC [GA]^GATC[CT] MspA1I 266 CAGCTG C[AC]G^C[GT]G MvaI 457 CCAGG CC^[AT]GG NdeII 802 GATC ^GATC NspV 443 TTCGAA TT^CGAA PaeR7I 545 CTCGAG C^TCGAG PleI 474 GACTC GAGTC(4/5) PstI 188 CTGCAG CTGCA^G PvuII 266 CAGCTG CAG^CTG Sau3AI 802 GATC ^GATC ScaI 588 AGTACT AGT^ACT SfuI 443 TTCGAA TT^CGAA SmlI 545 CTCGAG C^T[CT][GA]AG SspBI 118 TGTACA T^GTACA StyI 635 CCAAGG C^C[AT][AT]GG TfiI 177 GATTC G^A[AT]TC XhoI 545 CTCGAG C^TCGAG XhoII 801 AGATCC [GA]^GATC[CT] XmnI 446 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaI 44 CTCGGG C^[CT]CG[GA]G 545 CTCGAG AvaII 255 GGTCC G^G[AT]CC 903 GGTCC BfaI 341 CTAG C^TAG 641 CTAG BsaJI 25 CCCCGG C^C[ACGT][ACGT]GG 635 CCAAGG BsoBI 44 CTCGGG C^[CT]CG[GA]G 545 CTCGAG BstF5I 303 GGATG GGATG(2/0) 348 GGATG DdeI 145 CTAAG C^T[ACGT]AG 941 CTGAG EaeI 2 TGGCCG [CT]^GGCC[GA] 857 TGGCCG EcoRI 286 GAATTC G^AATTC 406 GAATTC FokI 303 GGATG GGATG(9/13) 348 GGATG HinfI 177 GATTC G^A[ACGT]TC 474 GACTC HphI 670 GGTGA GGTGA(8/7) 454 TCACC MaeI 341 CTAG C^TAG 641 CTAG MslI 478 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 761 CATCTGAATG SfaNI 550 GATGC GCATC(5/9) 814 GCATC TaqI 444 TCGA T^CGA 546 TCGA Tsp45I 324 GTCAC ^GT[GC]AC 511 GTGAC TthHB8I 444 TCGA T^CGA 546 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BcnI BsiYI BslI BsrI BssKI Cfr13I EarI Fnu4HI HaeIII HapII HpaII ItaI Ksp632I MaeIII MboII MnlI MseI MspI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfcI Tru9I TseI Tsp509I TspRI