(Circular) (Six-base) MAPSORT of: pFN21AE5352.seq Check: 5152 from: 1 to: 5650 pFN21AE5352 5650 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2026 2971 2026 Size: 945 4705 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2295 2527 2742 3713 5097 5365 1632 Size: 663 232 215 971 1384 268 1917 Fragments arranged by size: 1917 1384 971 663 268 232 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 2906 4379 2906 Size: 1473 4177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2291 2411 2835 2959 3185 3337 5446 1493 Size: 798 120 424 124 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 424 226 152 124 120 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2550 2975 1870 Size: 73 106 501 425 4545 Fragments arranged by size: 4545 501 425 106 73 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2971 4426 618 Size: 325 217 627 239 945 1455 1842 Fragments arranged by size: 1842 1455 945 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2969 1391 Size: 1578 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2346 928 Size: 971 447 4232 Fragments arranged by size: 4232 971 447 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4261 136 Size: 107 122 71 631 605 2589 1525 Fragments arranged by size: 2589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 3711 4867 1790 Size: 1921 1156 2573 Fragments arranged by size: 2573 1921 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3381 4339 Size: 416 53 83 399 24 1137 827 958 Cuts at: 4339 442 Size: 1753 Fragments arranged by size: 1753 1137 958 827 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2106 2106 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 4310 1239 Size: 818 2253 2579 Fragments arranged by size: 2579 2253 818 Bpu10I CC'TnA_GC Cuts at: 2150 2150 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2724 2754 1178 Size: 30 165 30 1321 30 4074 Fragments arranged by size: 4074 1321 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5650 BseYI C'CCAG_C Cuts at: 4853 5349 4853 Size: 496 5154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 1163 Size: 573 1078 3999 Fragments arranged by size: 3999 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3711 3796 4867 3711 Size: 85 1071 4494 Fragments arranged by size: 4494 1071 85 BsmI GAATG_Cn' Cuts at: 2777 3161 3254 2777 Size: 384 93 5173 Fragments arranged by size: 5173 384 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2969 3711 3796 4867 1391 Size: 399 1179 742 85 1071 2174 Fragments arranged by size: 2174 1179 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3005 844 Size: 2161 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 2123 96 Size: 2027 3623 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 2062 794 Size: 1268 4382 BstBI TT'CG_AA Cuts at: 2449 4454 2449 Size: 2005 3645 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2806 2980 3347 3732 3749 5201 5287 5299 Size: 834 174 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 834 385 367 346 174 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2574 2954 2574 Size: 380 5270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 4657 809 Size: 507 942 2399 1802 Fragments arranged by size: 2399 1802 942 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2862 3097 Size: 54 1425 50 275 18 177 855 235 Cuts at: 3097 3986 5615 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 855 275 235 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2546 2718 3580 1353 Size: 918 275 172 862 3423 Fragments arranged by size: 3423 918 862 275 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2295 2527 2742 3713 4310 5097 Size: 393 425 238 232 215 971 597 787 Cuts at: 5097 5365 1239 Size: 268 1524 Fragments arranged by size: 1524 971 787 597 425 393 268 238 232 215 EcoRI G'AATT_C Cuts at: 2291 2411 2959 3337 2291 Size: 120 548 378 4604 Fragments arranged by size: 4604 548 378 120 EcoRV GAT'ATC Cuts at: 2365 2365 Size: 5650 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2730 2762 2892 2924 4291 Size: 32 135 32 1158 32 130 32 1367 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1367 1158 135 130 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2030 2975 2030 Size: 945 4705 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2140 4764 4948 1305 Size: 774 61 2624 184 2007 Fragments arranged by size: 2624 2007 774 184 61 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2487 2770 3608 3967 4126 518 Size: 648 1321 283 838 359 159 2042 Fragments arranged by size: 2042 1321 838 648 359 283 159 MspA1I CmG'CkG Cuts at: 1606 2273 3066 3743 4891 5136 1606 Size: 667 793 677 1148 245 2120 Fragments arranged by size: 2120 1148 793 677 667 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2446 3719 3751 5232 5264 2414 Size: 32 1273 32 1481 32 2800 Fragments arranged by size: 2800 1481 1273 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2197 3002 830 Size: 1367 805 3478 Fragments arranged by size: 3478 1367 805 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2273 2273 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 2595 3898 5610 2595 Size: 1303 1712 2635 Fragments arranged by size: 2635 1712 1303 SfcI C'TryA_G Cuts at: 826 1045 2193 2811 2932 2998 4133 4814 5005 Size: 219 1148 618 121 66 1135 681 191 Cuts at: 5005 826 Size: 1471 Fragments arranged by size: 1471 1148 1135 681 618 219 191 121 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2550 3754 4655 4917 820 Size: 197 926 607 1204 901 262 1553 Fragments arranged by size: 1553 1204 926 901 607 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 3089 513 Size: 552 939 636 449 3074 Fragments arranged by size: 3074 939 636 552 449 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 3694 3847 4032 4477 325 Size: 1255 2097 17 153 185 445 1498 Fragments arranged by size: 2097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2123 2536 2593 Size: 274 80 33 51 1443 146 413 57 Cuts at: 2593 3896 5608 96 Size: 1303 1712 138 Fragments arranged by size: 1712 1443 1303 413 274 146 138 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 5610 5642 2724 Size: 32 2854 32 2732 Fragments arranged by size: 2854 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 2550 1943 Size: 607 5043 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 2455 3779 2455 Size: 1324 4326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI