(Circular) (Six-base) MAPSORT of: pFN21AE6565.seq Check: 7376 from: 1 to: 5224 pFN21AE6565 5224 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3034 278 Size: 53 83 186 936 1498 2468 Fragments arranged by size: 2468 1498 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2567 1937 Size: 630 4594 Acc65I G'GTAC_C Cuts at: 2545 2545 Size: 5224 AclI AA'CG_TT Cuts at: 1563 3351 3724 1563 Size: 1788 373 3063 Fragments arranged by size: 3063 1788 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2460 3287 4671 4939 1632 Size: 828 827 1384 268 1917 Fragments arranged by size: 1917 1384 828 827 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5027 AflIII A'CryG_T Cuts at: 1203 4123 5100 1203 Size: 2920 977 1327 Fragments arranged by size: 2920 1327 977 AgeI A'CCGG_T Cuts at: 4055 4055 Size: 5224 AhdI GACnn_n'nnGTC Cuts at: 3953 3953 Size: 5224 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2254 2493 1184 Size: 1070 239 3915 Fragments arranged by size: 3915 1070 239 AlwNI CAG_nnn'CTG Cuts at: 2967 4539 2967 Size: 1572 3652 ApaLI G'TGCA_C Cuts at: 3281 4437 3281 Size: 1156 4068 ApoI r'AATT_y Cuts at: 1493 2533 2759 2911 5020 1493 Size: 1040 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 226 152 AseI AT'TA_AT Cuts at: 160 1032 2454 3778 160 Size: 872 1422 1324 1606 Fragments arranged by size: 1606 1422 1324 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5224 AvaI C'yCGr_G Cuts at: 1870 1943 2549 1870 Size: 73 606 4545 Fragments arranged by size: 4545 606 73 BamHI G'GATC_C Cuts at: 2554 2921 2554 Size: 367 4857 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 4000 618 Size: 325 217 627 758 1455 1842 Fragments arranged by size: 1842 1455 758 627 325 217 BanII G_rGCy'C Cuts at: 1391 2221 2543 1391 Size: 830 322 4072 Fragments arranged by size: 4072 830 322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3415 3449 1903 Size: 34 1478 34 3678 Fragments arranged by size: 3678 1478 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2057 3116 4326 2057 Size: 1059 1210 2955 Fragments arranged by size: 2955 1210 1059 BclI T'GATC_A Cuts at: 1548 1548 Size: 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3835 136 Size: 107 122 71 631 605 2163 1525 Fragments arranged by size: 2163 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5219 5219 Size: 5224 BlpI GC'TnA_GC Cuts at: 2641 2641 Size: 5224 Bme1580I G_kGCm'C Cuts at: 1790 3285 4441 1790 Size: 1495 1156 2573 Fragments arranged by size: 2573 1495 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5224 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2955 3913 442 Size: 416 53 83 399 24 1538 958 1753 Fragments arranged by size: 1753 1538 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5224 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2237 2237 Size: 5224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 3884 1239 Size: 950 1695 2579 Fragments arranged by size: 2579 1695 950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3349 4214 4512 3349 Size: 865 298 4061 Fragments arranged by size: 4061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3887 882 Size: 617 2388 2219 Fragments arranged by size: 2388 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3015 493 Size: 711 1811 2702 Fragments arranged by size: 2702 1811 711 BsaBI GATnn'nnATC Cuts at: 5218 5218 Size: 5224 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3031 3413 4048 275 Size: 53 83 186 936 1498 382 635 1451 Fragments arranged by size: 1498 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3657 4055 4329 4476 4887 3657 Size: 398 274 147 411 3994 Fragments arranged by size: 3994 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4999 Fragments arranged by size: 4999 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2229 1186 Size: 1043 4181 BseYI C'CCAG_C Cuts at: 4427 4923 4427 Size: 496 4728 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2271 1163 Size: 573 535 4116 Fragments arranged by size: 4116 573 535 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2674 3435 4463 1540 Size: 275 186 673 761 1028 2301 Fragments arranged by size: 2301 1028 761 673 275 186 BsiHKAI G_wGCw'C Cuts at: 2019 2221 3285 3370 4441 2019 Size: 202 1064 85 1071 2802 Fragments arranged by size: 2802 1071 1064 202 85 BsmI GAATG_Cn' Cuts at: 2735 2828 2735 Size: 93 5131 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2221 2543 3285 3370 4441 1391 Size: 399 229 202 322 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 229 202 85 BspEI T'CCGG_A Cuts at: 4887 4887 Size: 5224 BspHI T'CATG_A Cuts at: 3113 4843 3113 Size: 1730 3494 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2579 844 Size: 1735 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3111 1394 Size: 429 1288 3507 Fragments arranged by size: 3507 1288 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2238 3719 3893 65 Size: 1183 990 1481 174 1396 Fragments arranged by size: 1481 1396 1183 990 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3868 4055 1700 Size: 110 2058 187 2869 Fragments arranged by size: 2869 2058 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5224 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5224 BssSI C'ACGA_G Cuts at: 3284 4296 3284 Size: 1012 4212 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5224 BstBI TT'CG_AA Cuts at: 4028 4028 Size: 5224 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5224 BstYI r'GATC_y Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219 Size: 582 367 385 17 1452 86 12 346 Cuts at: 5219 1972 Size: 1977 Fragments arranged by size: 1977 1452 582 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3733 Fragments arranged by size: 3733 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4485 Fragments arranged by size: 4485 683 56 BtsI GCAGTG_nn' Cuts at: 2740 3534 3554 2740 Size: 794 20 4410 Fragments arranged by size: 4410 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5224 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5189 DraI TTT'AAA Cuts at: 2058 2528 2058 Size: 470 4754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4231 809 Size: 507 2915 1802 Fragments arranged by size: 2915 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2671 3560 Size: 54 1425 50 275 18 270 571 889 Cuts at: 3560 5189 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 571 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2671 1537 Size: 275 859 4090 Fragments arranged by size: 4090 859 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2436 3154 1353 Size: 911 172 718 3423 Fragments arranged by size: 3423 911 718 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 3811 4185 4331 387 Size: 1064 138 459 1763 374 146 1280 Fragments arranged by size: 1763 1280 1064 459 374 146 138 EcoICRI GAG'CTC Cuts at: 2219 2219 Size: 5224 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2460 3287 3884 4671 4939 1239 Size: 393 557 271 827 597 787 268 1524 Fragments arranged by size: 1524 827 787 597 557 393 271 268 EcoO109I rG'GnC_Cy Cuts at: 2447 2447 Size: 5224 EcoRI G'AATT_C Cuts at: 2533 2911 2533 Size: 378 4846 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 5224 FspI TGC'GCA Cuts at: 3730 3730 Size: 5224 HaeII r_GCGC'y Cuts at: 1678 4371 1678 Size: 2693 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 3865 3897 3939 Size: 32 135 32 710 32 1551 32 42 Cuts at: 3939 3971 1373 Size: 32 2626 Fragments arranged by size: 2626 1551 710 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2568 2814 669 Size: 988 281 630 246 3079 Fragments arranged by size: 3079 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5224 HpaI GTT'AAC Cuts at: 2814 2814 Size: 5224 KpnI G_GTAC'C Cuts at: 2549 2549 Size: 5224 MfeI C'AATT_G Cuts at: 2823 2823 Size: 5224 MluI A'CGCG_T Cuts at: 5100 5100 Size: 5224 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2303 2420 4338 4522 1305 Size: 998 117 1918 184 2007 Fragments arranged by size: 2007 1918 998 184 117 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3402 Fragments arranged by size: 3402 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3182 3541 3700 518 Size: 648 2016 359 159 2042 Fragments arranged by size: 2042 2016 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2640 3317 4465 4710 1606 Size: 1034 677 1148 245 2120 Fragments arranged by size: 2120 1148 1034 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3733 Fragments arranged by size: 3733 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5224 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5224 NotI GC'GGCC_GC Cuts at: 2671 2671 Size: 5224 NspI r_CATG'y Cuts at: 1132 1229 2582 4127 1132 Size: 97 1353 1545 2229 Fragments arranged by size: 2229 1545 1353 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5224 PciI A'CATG_T Cuts at: 4123 4123 Size: 5224 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5224 PmeI GTTT'AAAC Cuts at: 2528 2528 Size: 5224 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2059 2091 3293 3325 4806 4838 2059 Size: 32 1202 32 1481 32 2445 Fragments arranged by size: 2445 1481 1202 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2447 2447 Size: 5224 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5224 PsiI TTA'TAA Cuts at: 2794 2794 Size: 5224 PstI C_TGCA'G Cuts at: 830 2576 830 Size: 1746 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5224 SacI G_AGCT'C Cuts at: 2221 2221 Size: 5224 SalI G'TCGA_C Cuts at: 1936 2566 1936 Size: 630 4594 SanDI GG'GwC_CC Cuts at: 2447 2447 Size: 5224 SapI GCTCTTCn'nnn_ Cuts at: 2264 2264 Size: 5224 SbfI CC_TGCA'GG Cuts at: 2576 2576 Size: 5224 ScaI AGT'ACT Cuts at: 3472 5184 3472 Size: 1712 3512 SfcI C'TryA_G Cuts at: 826 1045 2572 3707 4388 4579 826 Size: 219 1527 1135 681 191 1471 Fragments arranged by size: 1527 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5224 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5224 SmaI CCC'GGG Cuts at: 1872 2551 1872 Size: 679 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3328 4229 4491 820 Size: 197 926 1385 901 262 1553 Fragments arranged by size: 1553 1385 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3015 493 Size: 2522 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5224 SphI G_CATG'C Cuts at: 1132 2582 1132 Size: 1450 3774 SspI AAT'ATT Cuts at: 5 52 2458 3148 5 Size: 47 2406 690 2081 Fragments arranged by size: 2406 2081 690 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5224 StyI C'CwwG_G Cuts at: 513 1065 2004 2663 513 Size: 552 939 659 3074 Fragments arranged by size: 3074 939 659 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3251 3268 3421 3606 4051 325 Size: 1255 1671 17 153 185 445 1498 Fragments arranged by size: 1671 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3470 5182 96 Size: 274 80 33 51 1443 1493 1712 138 Fragments arranged by size: 1712 1493 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5184 5216 5184 Size: 32 5192 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5224 XbaI T'CTAG_A Cuts at: 2560 2560 Size: 5224 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5224 XmaI C'CCGG_G Cuts at: 1870 2549 1870 Size: 679 4545 XmnI GAAnn'nnTTC Cuts at: 3353 3353 Size: 5224 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3032 276 Size: 53 83 186 936 1498 2468 Fragments arranged by size: 2468 1498 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI