################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstF5I BstNI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeII MamI MflI MluI MluNI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 384 AAATTC [GA]^AATT[CT] AfaI 161 GTAC GT^AC AflII 645 CTTAAG C^TTAAG ApoI 384 AAATTC [GA]^AATT[CT] AseI 58 ATTAAT AT^TAAT AsnI 58 ATTAAT AT^TAAT AvaII 77 GGTCC G^G[AT]CC BfaI 277 CTAG C^TAG BfrI 645 CTTAAG C^TTAAG BsaI 187 GGTCTC GGTCTC(1/5) BsaJI 170 CCATGG C^C[ACGT][ACGT]GG BsmAI 188 GTCTC GTCTC(1/5) BstEII 606 GGTTACC G^GT[ACGT]ACC BstPI 606 GGTTACC G^GT[ACGT]ACC Cfr13I 77 GGTCC G^G[ACGT]CC DsaI 170 CCATGG C^C[GA][CT]GG EcoO65I 606 GGTTACC G^GT[ACGT]ACC EcoT14I 170 CCATGG C^C[AT][AT]GG HindIII 465 AAGCTT A^AGCTT HinfI 262 GATTC G^A[ACGT]TC HphI 325 GGTGA GGTGA(8/7) MaeI 277 CTAG C^TAG MfeI 7 CAATTG C^AATTG MnlI 64 GAGG CCTC(7/6) MunI 7 CAATTG C^AATTG NcoI 170 CCATGG C^CATGG NlaIII 171 CATG CATG^ PshBI 58 ATTAAT AT^TAAT RsaI 161 GTAC GT^AC Sau96I 77 GGTCC G^G[ACGT]CC SfaNI 376 GCATC GCATC(5/9) SfcI 46 CTACAG C^T[GA][CT]AG StyI 170 CCATGG C^C[AT][AT]GG TfiI 262 GATTC G^A[AT]TC TspRI 500 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AccI 44 GTCTAC GT^[AC][GT]AC 151 GTAGAC AluI 466 AGCT AG^CT 681 AGCT AlwNI 260 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 374 CAGCATCTG BbsI 411 GAAGAC GAAGAC(2/6) 495 GAAGAC BpuAI 411 GAAGAC GAAGAC(2/6) 495 GAAGAC TaqI 351 TCGA T^CGA 440 TCGA TthHB8I 351 TCGA T^CGA 440 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### DdeI DpnII HgaI MaeIII MboI MboII MseI NdeII Sau3AI SmlI Tru9I Tsp45I Tsp509I