################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvrII BamHI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BlnI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FseI HaeII Hin1I KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspBI StuI SwaI Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 71 enzymes) # ################################### Acc65I 711 GGTACC G^GTACC AflIII 920 ACATGT A^C[GA][CT]GT AgeI 500 ACCGGT A^CCGGT AlwNI 2735 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1981 CCTCAGG CC^T[ACGT]AGG Asp718I 711 GGTACC G^GTACC AspHI 2762 GAGCTC G[AT]GC[AT]^C AspI 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 61 CTCGAG C^[CT]CG[GA]G AviII 2658 TGCGCA TGC^GCA BalI 729 TGGCCA TGG^CCA BcgI 53 GCAAGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 48 GCTCAGC GC^T[ACGT]AGC Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC BsaAI 850 TACGTA [CT]AC^GT[GA] BsaI 497 GAGACC GGTCTC(1/5) BsaWI 500 ACCGGT [AT]^CCGG[AT] BsiHKAI 2762 GAGCTC G[AT]GC[AT]^C BsmI 1229 GCATTC GAATGC(1/-1) BsoBI 61 CTCGAG C^[CT]CG[GA]G BspHI 822 TCATGA T^CATGA BspLU11I 920 ACATGT A^CATGT BspMI 1947 ACCTGC ACCTGC(4/8) BsrBI 1912 GAGCGG CCGCTC(-3/-3) BsrDI 1106 CATTGC GCAATG(2/0) BsrFI 500 ACCGGT [GA]^CCGG[CT] Bst1107I 2439 GTATAC GTA^TAC BstXI 2597 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2439 GTATAC GTA^TAC Bsu36I 1981 CCTCAGG CC^T[ACGT]AGG CelII 48 GCTCAGC GC^T[ACGT]AGC CfoI 2659 GCGC GCG^C Cfr10I 500 ACCGGT [GA]^CCGG[CT] DraII 1081 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1348 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1981 CCTCAGG CC^T[ACGT]AGG EcoO109I 1081 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 2501 GAATTC G^AATTC EcoT14I 1673 CCTTGG C^C[AT][AT]GG FspI 2658 TGCGCA TGC^GCA HapII 501 CCGG C^CGG HgaI 2318 GACGC GACGC(5/10) HhaI 2659 GCGC GCG^C HinP1I 2659 GCGC G^CGC HincII 1678 GTTAAC GT[CT]^[GA]AC HindII 1678 GTTAAC GT[CT]^[GA]AC HindIII 1597 AAGCTT A^AGCTT HpaI 1678 GTTAAC GTT^AAC HpaII 501 CCGG C^CGG KpnI 711 GGTACC GGTAC^C MaeII 851 ACGT A^CGT MluNI 729 TGGCCA TGG^CCA MscI 729 TGGCCA TGG^CCA MslI 937 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 501 CCGG C^CGG NdeI 1280 CATATG CA^TATG PaeR7I 61 CTCGAG C^TCGAG PflFI 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 500 ACCGGT A^CCGGT PshAI 919 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 822 TCATGA T^CATGA SacI 2762 GAGCTC GAGCT^C SapI 340 GAAGAGC GCTCTTC(1/4) ScaI 1936 AGTACT AGT^ACT SnaBI 850 TACGTA TAC^GTA SphI 1356 GCATGC GCATG^C StyI 1673 CCTTGG C^C[AT][AT]GG Tsp45I 2419 GTCAC ^GT[GC]AC Tth111I 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 361 TCTAGA T^CTAGA XhoI 61 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccI 1992 GTCTAC GT^[AC][GT]AC 2439 GTATAC BanI 711 GGTACC G^G[CT][GA]CC 1714 GGTGCC BanII 64 GAGCCC G[GA]GC[CT]^C 2762 GAGCTC BglII 2640 AGATCT A^GATCT 2781 AGATCT BsgI 2045 CTGCAC GTGCAG(16/14) 2522 GTGCAG BsmFI 584 GGGAC GGGAC(10/14) 2594 GTCCC Cac8I 1356 GCATGC GC[ACGT]^[ACGT]GC 2403 GCAGGC EaeI 729 TGGCCA [CT]^GGCC[GA] 2532 TGGCCG EcoNI 1126 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1977 CCTTCCTCAGG NspI 920 ACATGT [GA]CATG^[CT] 1356 GCATGC SmlI 37 CTCAAG C^T[CT][GA]AG 61 CTCGAG SspI 2275 AATATT AAT^ATT 2815 AATATT TspRI 2018 CAGTG CA[GC]TG[ACGT][ACGT]^ 2421 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XhoII XmnI