(Circular) (Six-base) MAPSORT of: pFN21AE5765.seq Check: 2252 from: 1 to: 7654 pFN21AE5765 7654 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5464 278 Size: 53 83 186 936 3928 2468 Fragments arranged by size: 3928 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3998 4445 4997 1937 Size: 2061 447 552 4594 Fragments arranged by size: 4594 2061 552 447 Acc65I G'GTAC_C Cuts at: 2716 4975 2716 Size: 2259 5395 AclI AA'CG_TT Cuts at: 1563 5781 6154 1563 Size: 4218 373 3063 Fragments arranged by size: 4218 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2303 3330 3735 3960 4115 4254 4767 Size: 539 132 1027 405 225 155 139 513 Cuts at: 4767 5717 7101 7369 1632 Size: 950 1384 268 1917 Fragments arranged by size: 1917 1384 1027 950 539 513 405 268 225 155 139 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7457 AflIII A'CryG_T Cuts at: 1203 2925 6553 7530 1203 Size: 1722 3628 977 1327 Fragments arranged by size: 3628 1722 1327 977 AgeI A'CCGG_T Cuts at: 2505 6485 2505 Size: 3980 3674 AhdI GACnn_n'nnGTC Cuts at: 6383 6383 Size: 7654 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7654 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7654 AlwNI CAG_nnn'CTG Cuts at: 4745 5397 6969 4745 Size: 652 1572 5430 Fragments arranged by size: 5430 1572 652 ApaLI G'TGCA_C Cuts at: 5711 6867 5711 Size: 1156 6498 ApoI r'AATT_y Cuts at: 1493 2291 2822 3538 4161 4506 4865 4963 5189 Size: 798 531 716 623 345 359 98 226 Cuts at: 5189 5341 7450 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 798 716 623 531 359 345 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 6208 160 Size: 872 5176 1606 Fragments arranged by size: 5176 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7654 AvaI C'yCGr_G Cuts at: 1870 1943 2066 4979 1870 Size: 73 123 2913 4545 Fragments arranged by size: 4545 2913 123 73 BamHI G'GATC_C Cuts at: 4984 5351 4984 Size: 367 7287 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 3719 4975 6430 618 Size: 325 217 627 929 1003 1256 1455 1842 Fragments arranged by size: 1842 1455 1256 1003 929 627 325 217 BanII G_rGCy'C Cuts at: 1391 2073 4771 4973 1391 Size: 682 2698 202 4072 Fragments arranged by size: 4072 2698 682 202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2524 2838 3524 4322 928 Size: 971 625 314 686 798 4260 Fragments arranged by size: 4260 971 798 686 625 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2047 2081 5845 5879 1903 Size: 34 110 34 3764 34 3678 Fragments arranged by size: 3764 3678 110 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5546 6756 5546 Size: 1210 6444 BclI T'GATC_A Cuts at: 1548 2019 2167 2938 1548 Size: 471 148 771 6264 Fragments arranged by size: 6264 771 471 148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6265 136 Size: 107 122 71 631 605 4593 1525 Fragments arranged by size: 4593 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4645 4786 7649 4645 Size: 141 2863 4650 Fragments arranged by size: 4650 2863 141 BlpI GC'TnA_GC Cuts at: 2054 5071 2054 Size: 3017 4637 Bme1580I G_kGCm'C Cuts at: 1790 3724 5715 6871 1790 Size: 1934 1991 1156 2573 Fragments arranged by size: 2573 1991 1934 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7654 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3086 3114 Size: 416 53 83 399 24 969 700 28 Cuts at: 3114 5385 6343 442 Size: 2271 958 1753 Fragments arranged by size: 2271 1753 969 958 700 416 399 83 53 28 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7654 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 2688 3513 3522 3609 4229 4434 6314 Size: 1002 447 825 9 87 620 205 1880 Cuts at: 6314 1239 Size: 2579 Fragments arranged by size: 2579 1880 1002 825 620 447 205 87 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 5779 6644 6942 2027 Size: 3752 865 298 2739 Fragments arranged by size: 3752 2739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2496 6317 882 Size: 617 997 3821 2219 Fragments arranged by size: 3821 2219 997 617 BsaAI yAC'GTr Cuts at: 493 1204 2857 5445 493 Size: 711 1653 2588 2702 Fragments arranged by size: 2702 2588 1653 711 BsaBI GATnn'nnATC Cuts at: 2085 2469 2973 7648 2085 Size: 384 504 4675 2091 Fragments arranged by size: 4675 2091 504 384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5461 5843 6478 275 Size: 53 83 186 936 3928 382 635 1451 Fragments arranged by size: 3928 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 6087 6485 6759 6906 7317 2505 Size: 3582 398 274 147 411 2842 Fragments arranged by size: 3582 2842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2521 2539 2551 2569 3108 Size: 30 165 30 1118 18 12 18 539 Cuts at: 3108 3138 3835 3865 4407 4437 1178 Size: 30 697 30 542 30 4395 Fragments arranged by size: 4395 1118 697 542 539 165 30 30 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2244 2472 2934 3021 3177 3240 1186 Size: 1058 228 462 87 156 63 5600 Fragments arranged by size: 5600 1058 462 228 156 87 63 BseYI C'CCAG_C Cuts at: 2998 3922 6857 7353 2998 Size: 924 2935 496 3299 Fragments arranged by size: 3299 2935 924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4035 4548 1163 Size: 573 2299 513 4269 Fragments arranged by size: 4269 2299 573 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5104 5865 6893 1540 Size: 275 186 3103 761 1028 2301 Fragments arranged by size: 3103 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4771 5715 5800 6871 4771 Size: 944 85 1071 5554 Fragments arranged by size: 5554 1071 944 85 BsmI GAATG_Cn' Cuts at: 3234 5165 5258 3234 Size: 1931 93 5630 Fragments arranged by size: 5630 1931 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 3724 4771 4973 5715 5800 6871 Size: 399 283 1651 1047 202 742 85 1071 Cuts at: 6871 1391 Size: 2174 Fragments arranged by size: 2174 1651 1071 1047 742 399 283 202 85 BspEI T'CCGG_A Cuts at: 7317 7317 Size: 7654 BspHI T'CATG_A Cuts at: 2827 5543 7273 2827 Size: 2716 1730 3208 Fragments arranged by size: 3208 2716 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3961 5009 844 Size: 3117 1048 3489 Fragments arranged by size: 3489 3117 1048 BsrBI CCG'CTC Cuts at: 1394 1823 3919 5541 1394 Size: 429 2096 1622 3507 Fragments arranged by size: 3507 2096 1622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3110 6149 6323 65 Size: 1183 1862 3039 174 1396 Fragments arranged by size: 3039 1862 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2505 6298 6485 1700 Size: 110 695 3793 187 2869 Fragments arranged by size: 3793 2869 695 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7654 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7654 BssSI C'ACGA_G Cuts at: 5714 6726 5714 Size: 1012 6642 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7654 BstBI TT'CG_AA Cuts at: 6458 6458 Size: 7654 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7654 BstXI CCAn_nnnn'nTGG Cuts at: 4609 4609 Size: 7654 BstYI r'GATC_y Cuts at: 1972 2086 2644 2680 4645 4786 4984 5351 5736 Size: 114 558 36 1965 141 198 367 385 Cuts at: 5736 5753 7205 7291 7303 7649 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1965 1452 558 385 367 346 198 141 114 86 36 17 12 BstZ17I GTA'TAC Cuts at: 4446 4446 Size: 7654 Bsu36I CC'TnA_GG Cuts at: 3987 3987 Size: 7654 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6163 Fragments arranged by size: 6163 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6915 Fragments arranged by size: 6915 683 56 BtsI GCAGTG_nn' Cuts at: 5170 5964 5984 5170 Size: 794 20 6840 Fragments arranged by size: 6840 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7654 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7619 DraI TTT'AAA Cuts at: 4958 4958 Size: 7654 DraIII CAC_nnn'GTG Cuts at: 3358 3358 Size: 7654 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6661 809 Size: 507 5345 1802 Fragments arranged by size: 5345 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2734 4537 5101 Size: 54 1425 50 275 18 904 1803 564 Cuts at: 5101 5990 7619 8 Size: 889 1629 43 Fragments arranged by size: 1803 1629 1425 904 889 564 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5101 1537 Size: 275 3289 4090 Fragments arranged by size: 4090 3289 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2322 2340 2482 2716 3979 4869 4917 4945 Size: 969 18 142 234 1263 890 48 28 Cuts at: 4945 5584 1353 Size: 639 3423 Fragments arranged by size: 3423 1263 969 890 639 234 142 48 28 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2083 2542 6241 6615 6761 387 Size: 1064 138 494 459 3699 374 146 1280 Fragments arranged by size: 3699 1280 1064 494 459 374 146 138 EcoICRI GAG'CTC Cuts at: 4769 4769 Size: 7654 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2241 2303 2688 3330 3513 3522 Size: 393 539 70 62 385 642 183 9 Cuts at: 3522 3609 3735 3960 4115 4229 4254 4434 4767 Size: 87 126 225 155 114 25 180 333 Cuts at: 4767 5717 6314 7101 7369 1239 Size: 950 597 787 268 1524 Fragments arranged by size: 1524 950 787 642 597 539 393 385 333 268 225 183 180 155 126 114 87 70 62 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 3135 3986 3135 Size: 851 6803 EcoO109I rG'GnC_Cy Cuts at: 3087 3087 Size: 7654 EcoRI G'AATT_C Cuts at: 4506 4963 5341 4506 Size: 457 378 6819 Fragments arranged by size: 6819 457 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 4667 2594 Size: 2073 5581 FspI TGC'GCA Cuts at: 4665 6160 4665 Size: 1495 6159 HaeII r_GCGC'y Cuts at: 1678 6801 1678 Size: 5123 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2539 2571 2834 Size: 32 135 32 680 32 255 32 263 Cuts at: 2834 2866 3452 3484 4407 4439 4688 4720 4919 Size: 32 586 32 923 32 249 32 199 Cuts at: 4919 4951 6295 6327 6369 6401 1373 Size: 32 1344 32 42 32 2626 Fragments arranged by size: 2626 1344 923 680 586 263 255 249 199 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3685 4998 5244 669 Size: 988 281 1747 1313 246 3079 Fragments arranged by size: 3079 1747 1313 988 281 246 HindIII A'AGCT_T Cuts at: 748 3602 748 Size: 2854 4800 HpaI GTT'AAC Cuts at: 3685 5244 3685 Size: 1559 6095 KpnI G_GTAC'C Cuts at: 2720 4979 2720 Size: 2259 5395 MfeI C'AATT_G Cuts at: 5253 5253 Size: 7654 MluI A'CGCG_T Cuts at: 7530 7530 Size: 7654 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2543 6768 6952 1305 Size: 1238 4225 184 2007 Fragments arranged by size: 4225 2007 1238 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2736 10 Size: 54 1425 343 904 4928 Fragments arranged by size: 4928 1425 904 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2946 5612 5971 6130 518 Size: 648 1780 2666 359 159 2042 Fragments arranged by size: 2666 2042 1780 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 4037 4459 4754 4829 5070 5747 6895 Size: 567 1864 422 295 75 241 677 1148 Cuts at: 6895 7140 1606 Size: 245 2120 Fragments arranged by size: 2120 1864 1148 677 567 422 295 245 241 75 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6163 Fragments arranged by size: 6163 939 552 NdeI CA'TA_TG Cuts at: 387 3286 387 Size: 2899 4755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7654 NotI GC'GGCC_GC Cuts at: 5101 5101 Size: 7654 NspI r_CATG'y Cuts at: 1132 1229 2929 3365 5012 6557 1132 Size: 97 1700 436 1647 1545 2229 Fragments arranged by size: 2229 1700 1647 1545 436 97 PasI CC'CwG_GG Cuts at: 1348 4602 1348 Size: 3254 4400 PciI A'CATG_T Cuts at: 2925 6553 2925 Size: 3628 4026 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7654 PfoI T'CCnGG_A Cuts at: 3034 4318 3034 Size: 1284 6370 PmeI GTTT'AAAC Cuts at: 4958 4958 Size: 7654 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4515 4547 5723 5755 7236 7268 4515 Size: 32 1176 32 1481 32 4901 Fragments arranged by size: 4901 1481 1176 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2928 1613 Size: 1315 6339 PsiI TTA'TAA Cuts at: 3702 5224 3702 Size: 1522 6132 PstI C_TGCA'G Cuts at: 830 3280 3958 4084 4759 5006 830 Size: 2450 678 126 675 247 3478 Fragments arranged by size: 3478 2450 678 675 247 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7654 PvuII CAG'CTG Cuts at: 2173 4037 4459 4754 4829 2173 Size: 1864 422 295 75 4998 Fragments arranged by size: 4998 1864 422 295 75 SacI G_AGCT'C Cuts at: 4771 4771 Size: 7654 SalI G'TCGA_C Cuts at: 1936 4996 1936 Size: 3060 4594 SapI GCTCTTCn'nnn_ Cuts at: 2340 2340 Size: 7654 SbfI CC_TGCA'GG Cuts at: 5006 5006 Size: 7654 ScaI AGT'ACT Cuts at: 3943 5902 7614 3943 Size: 1959 1712 3983 Fragments arranged by size: 3983 1959 1712 SfcI C'TryA_G Cuts at: 826 1045 3219 3276 3954 4080 4755 5002 6137 Size: 219 2174 57 678 126 675 247 1135 Cuts at: 6137 6818 7009 826 Size: 681 191 1471 Fragments arranged by size: 2174 1471 1135 681 678 675 247 219 191 126 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7654 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7654 SmaI CCC'GGG Cuts at: 1872 4981 1872 Size: 3109 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2066 5758 6659 6921 820 Size: 197 926 99 24 3692 901 262 1553 Fragments arranged by size: 3692 1553 926 901 262 197 99 24 SnaBI TAC'GTA Cuts at: 493 2857 5445 493 Size: 2364 2588 2702 Fragments arranged by size: 2702 2588 2364 SpeI A'CTAG_T Cuts at: 152 152 Size: 7654 SphI G_CATG'C Cuts at: 1132 3365 5012 1132 Size: 2233 1647 3774 Fragments arranged by size: 3774 2233 1647 SspI AAT'ATT Cuts at: 5 52 4282 4822 5578 5 Size: 47 4230 540 756 2081 Fragments arranged by size: 4230 2081 756 540 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7654 StyI C'CwwG_G Cuts at: 513 1065 2004 3678 5093 513 Size: 552 939 1674 1415 3074 Fragments arranged by size: 3074 1674 1415 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2881 3133 3430 3845 5681 5698 5851 Size: 1255 1301 252 297 415 1836 17 153 Cuts at: 5851 6036 6481 325 Size: 185 445 1498 Fragments arranged by size: 1836 1498 1301 1255 445 415 297 252 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5900 7612 Size: 274 80 33 51 1443 1964 1959 1712 Cuts at: 7612 96 Size: 138 Fragments arranged by size: 1964 1959 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3108 3140 7614 7646 3108 Size: 32 4474 32 3116 Fragments arranged by size: 4474 3116 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4326 1316 Size: 3010 4644 XbaI T'CTAG_A Cuts at: 2366 4990 2366 Size: 2624 5030 XhoI C'TCGA_G Cuts at: 1943 2066 1943 Size: 123 7531 XmaI C'CCGG_G Cuts at: 1870 4979 1870 Size: 3109 4545 XmnI GAAnn'nnTTC Cuts at: 2295 2491 4510 4647 4866 5783 2295 Size: 196 2019 137 219 917 4166 Fragments arranged by size: 4166 2019 917 219 196 137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5462 276 Size: 53 83 186 936 3928 2468 Fragments arranged by size: 3928 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI