(Linear) (Six-base) MAPSORT of: KIEE5765.seq Check: 3286 from: 1 to: 2949 KIEE5765.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AccI GT'mk_AC Cuts at: 0 1993 2440 2949 Size: 1993 447 509 Fragments arranged by size: 1993 509 447 Acc65I G'GTAC_C Cuts at: 0 711 2949 Size: 711 2238 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 298 1325 1730 1955 2110 2249 2762 Size: 166 132 1027 405 225 155 139 513 Cuts at: 2762 2949 Size: 187 Fragments arranged by size: 1027 513 405 225 187 166 155 139 132 AflIII A'CryG_T Cuts at: 0 920 2949 Size: 920 2029 AgeI A'CCGG_T Cuts at: 0 500 2949 Size: 500 2449 AlwNI CAG_nnn'CTG Cuts at: 0 2740 2949 Size: 2740 209 ApoI r'AATT_y Cuts at: 0 286 817 1533 2156 2501 2860 2949 Size: 286 531 716 623 345 359 89 Fragments arranged by size: 716 623 531 359 345 286 89 AvaI C'yCGr_G Cuts at: 0 61 2949 Size: 61 2888 BanI G'GyrC_C Cuts at: 0 711 1714 2949 Size: 711 1003 1235 Fragments arranged by size: 1235 1003 711 BanII G_rGCy'C Cuts at: 0 68 2766 2949 Size: 68 2698 183 Fragments arranged by size: 2698 183 68 BbsI GAAGACnn'nnnn_ Cuts at: 0 519 833 1519 2317 2949 Size: 519 314 686 798 632 Fragments arranged by size: 798 686 632 519 314 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 42 76 2949 Size: 42 34 2873 Fragments arranged by size: 2873 42 34 BclI T'GATC_A Cuts at: 0 14 162 933 2949 Size: 14 148 771 2016 Fragments arranged by size: 2016 771 148 14 BglII A'GATC_T Cuts at: 0 2640 2781 2949 Size: 2640 141 168 Fragments arranged by size: 2640 168 141 BlpI GC'TnA_GC Cuts at: 0 49 2949 Size: 49 2900 Bme1580I G_kGCm'C Cuts at: 0 1719 2949 Size: 1719 1230 BmrI ACTGGGnnnn_n' Cuts at: 0 381 1081 1109 2949 Size: 381 700 28 1840 Fragments arranged by size: 1840 700 381 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 683 1508 1517 1604 2224 2429 2949 Size: 236 447 825 9 87 620 205 520 Fragments arranged by size: 825 620 520 447 236 205 87 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 22 2949 Size: 22 2927 BsaI GGTCTCn'nnnn_ Cuts at: 0 491 2949 Size: 491 2458 BsaAI yAC'GTr Cuts at: 0 852 2949 Size: 852 2097 BsaBI GATnn'nnATC Cuts at: 0 80 464 968 2949 Size: 80 384 504 1981 Fragments arranged by size: 1981 504 384 80 BsaWI w'CCGG_w Cuts at: 0 500 2949 Size: 500 2449 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 516 534 546 564 1103 1133 1830 1860 Size: 516 18 12 18 539 30 697 30 Cuts at: 1860 2402 2432 2949 Size: 542 30 517 Fragments arranged by size: 697 542 539 517 516 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 239 467 929 1016 1172 1235 2949 Size: 239 228 462 87 156 63 1714 Fragments arranged by size: 1714 462 239 228 156 87 63 BseYI C'CCAG_C Cuts at: 0 993 1917 2949 Size: 993 924 1032 Fragments arranged by size: 1032 993 924 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2030 2543 2949 Size: 2030 513 406 Fragments arranged by size: 2030 513 406 BsiHKAI G_wGCw'C Cuts at: 0 2766 2949 Size: 2766 183 BsmI GAATG_Cn' Cuts at: 0 1229 2949 Size: 1229 1720 Bsp1286I G_dGCh'C Cuts at: 0 68 1719 2766 2949 Size: 68 1651 1047 183 Fragments arranged by size: 1651 1047 183 68 BspHI T'CATG_A Cuts at: 0 822 2949 Size: 822 2127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1956 2949 Size: 1956 993 BsrBI CCG'CTC Cuts at: 0 1914 2949 Size: 1914 1035 BsrDI GCAATG_nn' Cuts at: 0 1105 2949 Size: 1105 1844 BsrFI r'CCGG_y Cuts at: 0 500 2949 Size: 500 2449 BstXI CCAn_nnnn'nTGG Cuts at: 0 2604 2949 Size: 2604 345 BstYI r'GATC_y Cuts at: 0 81 639 675 2640 2781 2949 Size: 81 558 36 1965 141 168 Fragments arranged by size: 1965 558 168 141 81 36 BstZ17I GTA'TAC Cuts at: 0 2441 2949 Size: 2441 508 Bsu36I CC'TnA_GG Cuts at: 0 1982 2949 Size: 1982 967 DraIII CAC_nnn'GTG Cuts at: 0 1353 2949 Size: 1353 1596 EaeI y'GGCC_r Cuts at: 0 729 2532 2949 Size: 729 1803 417 Fragments arranged by size: 1803 729 417 EarI CTCTTCn'nnn_ Cuts at: 0 317 335 477 711 1974 2864 2912 2949 Size: 317 18 142 234 1263 890 48 37 Fragments arranged by size: 1263 890 317 234 142 48 37 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 78 537 2949 Size: 78 459 2412 Fragments arranged by size: 2412 459 78 EcoICRI GAG'CTC Cuts at: 0 2764 2949 Size: 2764 185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 166 236 298 683 1325 1508 1517 1604 Size: 166 70 62 385 642 183 9 87 Cuts at: 1604 1730 1955 2110 2224 2249 2429 2762 2949 Size: 126 225 155 114 25 180 333 187 Fragments arranged by size: 642 385 333 225 187 183 180 166 155 126 114 87 70 62 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1130 1981 2949 Size: 1130 851 968 Fragments arranged by size: 1130 968 851 EcoO109I rG'GnC_Cy Cuts at: 0 1082 2949 Size: 1082 1867 EcoRI G'AATT_C Cuts at: 0 2501 2949 Size: 2501 448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 589 2662 2949 Size: 589 2073 287 Fragments arranged by size: 2073 589 287 FspI TGC'GCA Cuts at: 0 2660 2949 Size: 2660 289 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 534 566 829 861 1447 1479 Size: 247 32 255 32 263 32 586 32 Cuts at: 1479 2402 2434 2683 2715 2914 2946 2949 Size: 923 32 249 32 199 32 3 Fragments arranged by size: 923 586 263 255 249 247 199 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 0 1680 2949 Size: 1680 1269 HindIII A'AGCT_T Cuts at: 0 1597 2949 Size: 1597 1352 HpaI GTT'AAC Cuts at: 0 1680 2949 Size: 1680 1269 KpnI G_GTAC'C Cuts at: 0 715 2949 Size: 715 2234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 538 2949 Size: 538 2411 MscI TGG'CCA Cuts at: 0 731 2949 Size: 731 2218 MslI CAynn'nnrTG Cuts at: 0 941 2949 Size: 941 2008 MspA1I CmG'CkG Cuts at: 0 168 2032 2454 2749 2824 2949 Size: 168 1864 422 295 75 125 Fragments arranged by size: 1864 422 295 168 125 75 NdeI CA'TA_TG Cuts at: 0 1281 2949 Size: 1281 1668 NspI r_CATG'y Cuts at: 0 924 1360 2949 Size: 924 436 1589 Fragments arranged by size: 1589 924 436 PasI CC'CwG_GG Cuts at: 0 2597 2949 Size: 2597 352 PciI A'CATG_T Cuts at: 0 920 2949 Size: 920 2029 PfoI T'CCnGG_A Cuts at: 0 1029 2313 2949 Size: 1029 1284 636 Fragments arranged by size: 1284 1029 636 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2510 2542 2949 Size: 2510 32 407 Fragments arranged by size: 2510 407 32 PshAI GACnn'nnGTC Cuts at: 0 923 2949 Size: 923 2026 PsiI TTA'TAA Cuts at: 0 1697 2949 Size: 1697 1252 PstI C_TGCA'G Cuts at: 0 1275 1953 2079 2754 2949 Size: 1275 678 126 675 195 Fragments arranged by size: 1275 678 675 195 126 PvuII CAG'CTG Cuts at: 0 168 2032 2454 2749 2824 2949 Size: 168 1864 422 295 75 125 Fragments arranged by size: 1864 422 295 168 125 75 SacI G_AGCT'C Cuts at: 0 2766 2949 Size: 2766 183 SapI GCTCTTCn'nnn_ Cuts at: 0 335 2949 Size: 335 2614 ScaI AGT'ACT Cuts at: 0 1938 2949 Size: 1938 1011 SfcI C'TryA_G Cuts at: 0 1214 1271 1949 2075 2750 2949 Size: 1214 57 678 126 675 199 Fragments arranged by size: 1214 678 675 199 126 57 SmlI C'TyrA_G Cuts at: 0 37 61 2949 Size: 37 24 2888 Fragments arranged by size: 2888 37 24 SnaBI TAC'GTA Cuts at: 0 852 2949 Size: 852 2097 SphI G_CATG'C Cuts at: 0 1360 2949 Size: 1360 1589 SspI AAT'ATT Cuts at: 0 2277 2817 2949 Size: 2277 540 132 Fragments arranged by size: 2277 540 132 StyI C'CwwG_G Cuts at: 0 1673 2949 Size: 1673 1276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 876 1128 1425 1840 2949 Size: 876 252 297 415 1109 Fragments arranged by size: 1109 876 415 297 252 TatI w'GTAC_w Cuts at: 0 1936 2949 Size: 1936 1013 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1103 1135 2949 Size: 1103 32 1814 Fragments arranged by size: 1814 1103 32 Tth111I GACn'n_nGTC Cuts at: 0 2321 2949 Size: 2321 628 XbaI T'CTAG_A Cuts at: 0 361 2949 Size: 361 2588 XhoI C'TCGA_G Cuts at: 0 61 2949 Size: 61 2888 XmnI GAAnn'nnTTC Cuts at: 0 290 486 2505 2642 2861 2949 Size: 290 196 2019 137 219 88 Fragments arranged by size: 2019 290 219 196 137 88 Enzymes that do cut: AccI Acc65I AcuI AflIII AgeI AlwNI ApoI AvaI BanI BanII BbsI BcgI BclI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I NdeI NspI PasI PciI PfoI PpiI PshAI PsiI PstI PvuII SacI SapI ScaI SfcI SmlI SnaBI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BmgBI BmtI BplI Bpu10I BsaHI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoRV FseI FspAI HaeII KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI StuI SwaI XcmI XmaI ZraI