################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbvCI BcgI BclI BfrI BlnI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BspLU11I BssHII BstAPI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NspI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 611 GACGTC GACGT^C AflIII 266 ACGTGT A^C[GA][CT]GT AocI 444 CCTGAGG CC^T[ACGT]AGG Asp700I 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1670 TGGCCA TGG^CCA BbeI 1997 GGCGCC GGCGC^C Bsp1407I 1925 TGTACA T^GTACA BsrBI 483 CCGCTC CCGCTC(-3/-3) BsrGI 1925 TGTACA T^GTACA Bst1107I 2185 GTATAC GTA^TAC BstBI 2804 TTCGAA TT^CGAA BstEII 2217 GGTGACC G^GT[ACGT]ACC BstPI 2217 GGTGACC G^GT[ACGT]ACC BstXI 1107 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2185 GTATAC GTA^TAC Bsu36I 444 CCTGAGG CC^T[ACGT]AGG DrdI 611 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 444 CCTGAGG CC^T[ACGT]AGG EcoO65I 2217 GGTGACC G^GT[ACGT]ACC EcoRI 2010 GAATTC G^AATTC EcoRV 1309 GATATC GAT^ATC EcoT22I 1249 ATGCAT ATGCA^T HgaI 718 GCGTC GACGC(5/10) HincII 2263 GTCAAC GT[CT]^[GA]AC HindII 2263 GTCAAC GT[CT]^[GA]AC KasI 1997 GGCGCC G^GCGCC MluNI 1670 TGGCCA TGG^CCA MscI 1670 TGGCCA TGG^CCA MseI 1937 TTAA T^TAA NarI 1997 GGCGCC GG^CGCC NsiI 1249 ATGCAT ATGCA^T NspV 2804 TTCGAA TT^CGAA PflMI 2451 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 2628 GCTCTTC GCTCTTC(1/4) ScaI 1790 AGTACT AGT^ACT SfoI 1997 GGCGCC GGC^GCC SfuI 2804 TTCGAA TT^CGAA SmlI 2410 CTCAAG C^T[CT][GA]AG SspBI 1925 TGTACA T^GTACA Tru9I 1937 TTAA T^TAA Van91I 2451 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 722 CCAACGTGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 560 GTCTAC GT^[AC][GT]AC 2185 GTATAC AccIII 66 TCCGGA T^CCGGA 1034 TCCGGA AcyI 611 GACGTC G[GA]^CG[CT]C 1997 GGCGCC AlwNI 86 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2452 CAGTTTCTG ApaI 1552 GGGCCC GGGCC^C 2915 GGGCCC AvaI 555 CCCGAG C^[CT]CG[GA]G 1518 CTCGGG BamHI 2693 GGATCC G^GATCC 2741 GGATCC BbrPI 265 CACGTG CAC^GTG 2677 CACGTG BglI 1894 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2127 GCCAACGCGGC BglII 960 AGATCT A^GATCT 3031 AGATCT BlpI 91 GCTGAGC GC^T[ACGT]AGC 1965 GCTGAGC Bpu1102I 91 GCTGAGC GC^T[ACGT]AGC 1965 GCTGAGC BsaHI 611 GACGTC G[GA]^CG[CT]C 1997 GGCGCC BseAI 66 TCCGGA T^CCGGA 1034 TCCGGA BsgI 283 CTGCAC GTGCAG(16/14) 2713 GTGCAG BsoBI 555 CCCGAG C^[CT]CG[GA]G 1518 CTCGGG BspEI 66 TCCGGA T^CCGGA 1034 TCCGGA BspHI 689 TCATGA T^CATGA 2780 TCATGA BspMI 307 ACCTGC ACCTGC(4/8) 581 ACCTGC BssSI 2920 CACGAG CACGAG(-5/-1) 730 CTCGTG CelII 91 GCTGAGC GC^T[ACGT]AGC 1965 GCTGAGC HaeII 1997 GGCGCC [GA]GCGC^[CT] 2037 GGCGCT Hin1I 611 GACGTC G[GA]^CG[CT]C 1997 GGCGCC MroI 66 TCCGGA T^CCGGA 1034 TCCGGA NaeI 1207 GCCGGC GCC^GGC 2296 GCCGGC NcoI 1490 CCATGG C^CATGG 2975 CCATGG NgoMIV 1207 GCCGGC G^CCGGC 2296 GCCGGC PmaCI 265 CACGTG CAC^GTG 2677 CACGTG PmlI 265 CACGTG CAC^GTG 2677 CACGTG PstI 166 CTGCAG CTGCA^G 313 CTGCAG PvuII 86 CAGCTG CAG^CTG 1646 CAGCTG RcaI 689 TCATGA T^CATGA 2780 TCATGA SfcI 166 CTGCAG C^T[GA][CT]AG 313 CTGCAG TfiI 2510 GAATC G^A[AT]TC 2970 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII