Restriction map for
pFN21AE6765
(Circular) (Six-base) MAPSORT of: pFN21AE6765.seq Check: 4230 from: 1 to: 7762
pFN21AE6765 7762 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2620 5572 278
Size: 53 83 186 936 1084 2952 2468
Fragments arranged by size:
2952 2468 1084 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2566 4191 5105 1937
Size: 629 1625 914 4594
Fragments arranged by size:
4594 1625 914 629
Acc65I G'GTAC_C
Cuts at: 5083 5083
Size: 7762
AclI AA'CG_TT
Cuts at: 1563 5889 6262 1563
Size: 4326 373 3063
Fragments arranged by size:
4326 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2914 3577 3879 4115 4310 5825 7209 7477
Size: 1282 663 302 236 195 1515 1384 268
Cuts at: 7477 1632
Size: 1917
Fragments arranged by size:
1917 1515 1384 1282 663 302 268 236
195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7565
AflIII A'CryG_T
Cuts at: 1203 2271 6661 7638 1203
Size: 1068 4390 977 1327
Fragments arranged by size:
4390 1327 1068 977
AgeI A'CCGG_T
Cuts at: 6593 6593
Size: 7762
AhdI GACnn_n'nnGTC
Cuts at: 6491 6491
Size: 7762
AleI CACnn'nnGTG
Cuts at: 1166 4715 1166
Size: 3549 4213
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4272 1184
Size: 3088 4674
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4934 4966 4934
Size: 32 7730
AlwNI CAG_nnn'CTG
Cuts at: 2096 4462 5505 7077 2096
Size: 2366 1043 1572 2781
Fragments arranged by size:
2781 2366 1572 1043
ApaI G_GGCC'C
Cuts at: 3561 4924 3561
Size: 1363 6399
ApaLI G'TGCA_C
Cuts at: 5819 6975 5819
Size: 1156 6606
ApoI r'AATT_y
Cuts at: 1493 2690 2987 4015 4106 5071 5297 5449 7558
Size: 1197 297 1028 91 965 226 152 2109
Cuts at: 7558 1493
Size: 1697
Fragments arranged by size:
2109 1697 1197 1028 965 297 226 152
91
AseI AT'TA_AT
Cuts at: 160 1032 6316 160
Size: 872 5284 1606
Fragments arranged by size:
5284 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
AvaI C'yCGr_G
Cuts at: 1870 1943 2560 3523 5087 1870
Size: 73 617 963 1564 4545
Fragments arranged by size:
4545 1564 963 617 73
BamHI G'GATC_C
Cuts at: 4698 4746 5092 5459 4698
Size: 48 346 367 7001
Fragments arranged by size:
7001 367 346 48
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2457 2821 3061 3785 3907
Size: 325 217 627 670 364 240 724 122
Cuts at: 3907 4002 5083 6538 618
Size: 95 1081 1455 1842
Fragments arranged by size:
1842 1455 1081 724 670 627 364 325
240 217 122 95
BanII G_rGCy'C
Cuts at: 1391 2307 2424 2549 3051 3148 3561 4365 4924
Size: 916 117 125 502 97 413 804 559
Cuts at: 4924 4947 5081 1391
Size: 23 134 4072
Fragments arranged by size:
4072 916 804 559 502 413 134 125
117 97 23
BbeI G_GCGC'C
Cuts at: 4006 4006
Size: 7762
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 4492 4516 4567 928
Size: 971 690 1903 24 51 4123
Fragments arranged by size:
4123 1903 971 690 51 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5953 5987 1903
Size: 34 4016 34 3678
Fragments arranged by size:
4016 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2158 4559 5024 5654 6864 2158
Size: 2401 465 630 1210 3056
Fragments arranged by size:
3056 2401 1210 630 465
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7762
BfrBI ATG'CAT
Cuts at: 3256 3256
Size: 7762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3905 4138 6373
Size: 107 122 71 631 605 2233 233 2235
Cuts at: 6373 136
Size: 1525
Fragments arranged by size:
2235 2233 1525 631 605 233 122 107
71
BglII A'GATC_T
Cuts at: 2965 5036 7757 2965
Size: 2071 2721 2970
Fragments arranged by size:
2970 2721 2071
BlpI GC'TnA_GC
Cuts at: 2097 3971 5179 2097
Size: 1874 1208 4680
Fragments arranged by size:
4680 1874 1208
Bme1580I G_kGCm'C
Cuts at: 1790 3066 3491 3561 4441 4924 5823 6979 1790
Size: 1276 425 70 880 483 899 1156 2573
Fragments arranged by size:
2573 1276 1156 899 880 483 425 70
BmgBI CAC'GTC
Cuts at: 1318 2938 1318
Size: 1620 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2864 4450 4942
Size: 416 53 83 399 24 1447 1586 492
Cuts at: 4942 5493 6451 442
Size: 551 958 1753
Fragments arranged by size:
1753 1586 1447 958 551 492 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3748 4595 3748
Size: 847 6915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2278 3039 3171 6422 1239
Size: 851 188 761 132 3251 2579
Fragments arranged by size:
3251 2579 851 761 188 132
Bpu10I CC'TnA_GC
Cuts at: 2494 2494
Size: 7762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4400 5887 6752 7050 4400
Size: 1487 865 298 5112
Fragments arranged by size:
5112 1487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2178 2411 4424 6425 882
Size: 617 679 233 2013 2001 2219
Fragments arranged by size:
2219 2013 2001 679 617 233
BsaAI yAC'GTr
Cuts at: 493 1204 2272 4375 4684 5553 493
Size: 711 1068 2103 309 869 2702
Fragments arranged by size:
2702 2103 1068 869 711 309
BsaBI GATnn'nnATC
Cuts at: 7756 7756
Size: 7762
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2617 4003 5569 5951
Size: 53 83 186 936 1084 1386 1566 382
Cuts at: 5951 6586 275
Size: 635 1451
Fragments arranged by size:
1566 1451 1386 1084 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2071 3039 4272 6195 6593 6867 7014 7425 2071
Size: 968 1233 1923 398 274 147 411 2408
Fragments arranged by size:
2408 1923 1233 968 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 2536 2566 4934
Size: 30 165 30 936 30 167 30 2368
Cuts at: 4934 4964 1178
Size: 30 3976
Fragments arranged by size:
3976 2368 936 167 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2077 2467 2823 3147 3168 3572 3737
Size: 847 44 390 356 324 21 404 165
Cuts at: 3737 4947 4955 1186
Size: 1210 8 3993
Fragments arranged by size:
3993 1210 847 404 390 356 324 165
44 21 8
BseYI C'CCAG_C
Cuts at: 2027 2089 2803 3106 4195 4836 6965 7461 2027
Size: 62 714 303 1089 641 2129 496 2328
Fragments arranged by size:
2328 2129 1089 714 641 496 303 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 4739 1163
Size: 573 537 2466 4186
Fragments arranged by size:
4186 2466 573 537
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5212 5973 7001 1540
Size: 275 186 3211 761 1028 2301
Fragments arranged by size:
3211 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2424 2736 3148 4352 4743 5823 5908 6979
Size: 117 312 412 1204 391 1080 85 1071
Cuts at: 6979 2307
Size: 3090
Fragments arranged by size:
3090 1204 1080 1071 412 391 312 117
85
BsmI GAATG_Cn'
Cuts at: 5273 5366 5273
Size: 93 7669
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2424 2549 2736 3051 3066 3148
Size: 399 517 117 125 187 315 15 82
Cuts at: 3148 3491 3561 4352 4365 4441 4743 4924 4947
Size: 343 70 791 13 76 302 181 23
Cuts at: 4947 5081 5823 5908 6979 1391
Size: 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 791 742 517 399 343 315
302 187 181 134 125 117 85 82
76 70 23 15 13
BspEI T'CCGG_A
Cuts at: 2071 3039 7425 2071
Size: 968 4386 2408
Fragments arranged by size:
4386 2408 968
BspHI T'CATG_A
Cuts at: 2694 4785 5651 7381 2694
Size: 2091 866 1730 3075
Fragments arranged by size:
3075 2091 1730 866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 2595 5117 844
Size: 1477 274 2522 3489
Fragments arranged by size:
3489 2522 1477 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2490 5649 1394
Size: 429 667 3159 3507
Fragments arranged by size:
3507 3159 667 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2901 3166 3610 5022 6257 6431 65
Size: 1183 1653 265 444 1412 1235 174 1396
Fragments arranged by size:
1653 1412 1396 1235 1183 444 265 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3212 4301 4487 4558 6406 6593 1700
Size: 110 1402 1089 186 71 1848 187 2869
Fragments arranged by size:
2869 1848 1402 1089 187 186 110 71
BsrGI T'GTAC_A
Cuts at: 96 3930 96
Size: 3834 3928
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7762
BssSI C'ACGA_G
Cuts at: 2735 4925 5822 6834 2735
Size: 2190 897 1012 3663
Fragments arranged by size:
3663 2190 1012 897
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7762
BstBI TT'CG_AA
Cuts at: 4810 6566 4810
Size: 1756 6006
BstEII G'GTnAC_C
Cuts at: 1184 4222 1184
Size: 3038 4724
BstXI CCAn_nnnn'nTGG
Cuts at: 3119 3119
Size: 7762
BstYI r'GATC_y
Cuts at: 1972 2703 2965 4698 4722 4746 4999 5036 5092
Size: 731 262 1733 24 24 253 37 56
Cuts at: 5092 5459 5844 5861 7313 7399 7411 7757 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1733 1452 731 385 367 346 262
253 86 56 37 24 24 17 12
BstZ17I GTA'TAC
Cuts at: 4192 4192
Size: 7762
Bsu36I CC'TnA_GG
Cuts at: 2450 2450
Size: 7762
BtgI C'CryG_G
Cuts at: 513 1065 2577 3495 4181 4383 4758 4980 513
Size: 552 1512 918 686 202 375 222 3295
Fragments arranged by size:
3295 1512 918 686 552 375 222 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2755 4381 487
Size: 683 56 1529 1626 3868
Fragments arranged by size:
3868 1626 1529 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3208 3489 4254 5017 5278 6072 6092 3190
Size: 18 281 765 763 261 794 20 4860
Fragments arranged by size:
4860 794 765 763 281 261 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7762
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3148 3183 529
Size: 35 2584 35 5108
Fragments arranged by size:
5108 2584 35 35
DraI TTT'AAA
Cuts at: 5066 5066
Size: 7762
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2622 6769 809
Size: 507 1306 4147 1802
Fragments arranged by size:
4147 1802 1306 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2414 2796 2893
Size: 54 1425 50 275 18 584 382 97
Cuts at: 2893 3675 5209 6098 7727 8
Size: 782 1534 889 1629 43
Fragments arranged by size:
1629 1534 1425 889 782 584 382 275
97 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5209 1537
Size: 275 3397 4090
Fragments arranged by size:
4090 3397 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 2933 3524 3590 4640 4712 4895 4901
Size: 950 630 591 66 1050 72 183 6
Cuts at: 4901 5692 1353
Size: 791 3423
Fragments arranged by size:
3423 1050 950 791 630 591 183 72
66 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3156 3231 3617 6349 6723 6869
Size: 1064 138 1567 75 386 2732 374 146
Cuts at: 6869 387
Size: 1280
Fragments arranged by size:
2732 1567 1280 1064 386 374 146 138
75
EcoICRI GAG'CTC
Cuts at: 2305 2422 3146 2305
Size: 117 724 6921
Fragments arranged by size:
6921 724 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2278 2914 3039 3171 3577 3879
Size: 393 458 188 636 125 132 406 302
Cuts at: 3879 4115 4310 5825 6422 7209 7477 1239
Size: 236 195 1515 597 787 268 1524
Fragments arranged by size:
1524 1515 787 636 597 458 406 393
302 268 236 195 188 132 125
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 3153 4146 2120
Size: 1033 993 5736
Fragments arranged by size:
5736 1033 993
EcoO109I rG'GnC_Cy
Cuts at: 2085 2858 3028 3557 4920 2085
Size: 773 170 529 1363 4927
Fragments arranged by size:
4927 1363 773 529 170
EcoRI G'AATT_C
Cuts at: 4015 5071 5449 4015
Size: 1056 378 6328
Fragments arranged by size:
6328 1056 378
EcoRV GAT'ATC
Cuts at: 3316 3316
Size: 7762
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3601 3844 3601
Size: 243 7519
FspI TGC'GCA
Cuts at: 6268 6268
Size: 7762
HaeII r_GCGC'y
Cuts at: 1678 4006 4046 6909 1678
Size: 2328 40 2863 2531
Fragments arranged by size:
2863 2531 2328 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2339 2371 3519
Size: 32 135 32 580 32 155 32 1148
Cuts at: 3519 3551 6403 6435 6477 6509 1373
Size: 32 2852 32 42 32 2626
Fragments arranged by size:
2852 2626 1148 580 155 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4270 5106 5352 669
Size: 988 281 2332 836 246 3079
Fragments arranged by size:
3079 2332 988 836 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7762
HpaI GTT'AAC
Cuts at: 5352 5352
Size: 7762
KasI G'GCGC_C
Cuts at: 4002 4002
Size: 7762
KpnI G_GTAC'C
Cuts at: 5087 5087
Size: 7762
MfeI C'AATT_G
Cuts at: 5361 5361
Size: 7762
MluI A'CGCG_T
Cuts at: 7638 7638
Size: 7762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2751 3936 5027 6876 7060 1305
Size: 1446 1185 1091 1849 184 2007
Fragments arranged by size:
2007 1849 1446 1185 1091 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3677 10
Size: 54 1425 343 1845 4095
Fragments arranged by size:
4095 1845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3494 4337 4589 4715 5720 6079
Size: 648 843 1485 843 252 126 1005 359
Cuts at: 6079 6238 518
Size: 159 2042
Fragments arranged by size:
2042 1485 1005 843 843 648 359 252
159 126
MspA1I CmG'CkG
Cuts at: 1606 2093 2680 3228 3653 4690 5178 5855 7003
Size: 487 587 548 425 1037 488 677 1148
Cuts at: 7003 7248 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1037 677 587 548 488 487
425 245
NaeI GCC'GGC
Cuts at: 3214 4303 3214
Size: 1089 6673
NarI GG'CG_CC
Cuts at: 4003 4003
Size: 7762
NcoI C'CATG_G
Cuts at: 513 1065 3495 4980 513
Size: 552 2430 1485 3295
Fragments arranged by size:
3295 2430 1485 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7762
NgoMIV G'CCGG_C
Cuts at: 3212 4301 3212
Size: 1089 6673
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7762
NotI GC'GGCC_GC
Cuts at: 5209 5209
Size: 7762
NsiI A_TGCA'T
Cuts at: 3258 3258
Size: 7762
NspI r_CATG'y
Cuts at: 1132 1229 5120 6665 1132
Size: 97 3891 1545 2229
Fragments arranged by size:
3891 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4442 1348
Size: 3094 4668
PciI A'CATG_T
Cuts at: 6661 6661
Size: 7762
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4462 1108
Size: 3354 4408
PfoI T'CCnGG_A
Cuts at: 2106 2424 3016 3148 2106
Size: 318 592 132 6720
Fragments arranged by size:
6720 592 318 132
PmeI GTTT'AAAC
Cuts at: 5066 5066
Size: 7762
PmlI CAC'GTG
Cuts at: 2272 4684 2272
Size: 2412 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4934 4966 5831 5863 7344 7376 4934
Size: 32 865 32 1481 32 5320
Fragments arranged by size:
5320 1481 865 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2085 2858 3028 2085
Size: 773 170 6819
Fragments arranged by size:
6819 773 170
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7762
PsiI TTA'TAA
Cuts at: 4970 5332 4970
Size: 362 7400
PspOMI G'GGCC_C
Cuts at: 3557 4920 3557
Size: 1363 6399
PstI C_TGCA'G
Cuts at: 830 2175 2322 5114 830
Size: 1345 147 2792 3478
Fragments arranged by size:
3478 2792 1345 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7762
PvuII CAG'CTG
Cuts at: 2093 3653 2093
Size: 1560 6202
SacI G_AGCT'C
Cuts at: 2307 2424 3148 2307
Size: 117 724 6921
Fragments arranged by size:
6921 724 117
SalI G'TCGA_C
Cuts at: 1936 5104 1936
Size: 3168 4594
SanDI GG'GwC_CC
Cuts at: 2085 3028 2085
Size: 943 6819
SapI GCTCTTCn'nnn_
Cuts at: 4640 4640
Size: 7762
SbfI CC_TGCA'GG
Cuts at: 5114 5114
Size: 7762
ScaI AGT'ACT
Cuts at: 3797 6010 7722 3797
Size: 2213 1712 3837
Fragments arranged by size:
3837 2213 1712
SfcI C'TryA_G
Cuts at: 826 1045 2171 2318 5110 6245 6926 7117 826
Size: 219 1126 147 2792 1135 681 191 1471
Fragments arranged by size:
2792 1471 1135 1126 681 219 191 147
SfoI GGC'GCC
Cuts at: 4004 4004
Size: 7762
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7762
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7762
SmaI CCC'GGG
Cuts at: 1872 5089 1872
Size: 3217 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4415 5866 6767 7029 820
Size: 197 926 2472 1451 901 262 1553
Fragments arranged by size:
2472 1553 1451 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5553 493
Size: 5060 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7762
SphI G_CATG'C
Cuts at: 1132 5120 1132
Size: 3988 3774
SspI AAT'ATT
Cuts at: 5 52 5686 5
Size: 47 5634 2081
Fragments arranged by size:
5634 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7762
StyI C'CwwG_G
Cuts at: 513 1065 2709 3495 4980 5201 513
Size: 552 1644 786 1485 221 3074
Fragments arranged by size:
3074 1644 1485 786 552 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 4161 4315 5789 5806 5959 6144
Size: 1255 1251 1330 154 1474 17 153 185
Cuts at: 6144 6589 325
Size: 445 1498
Fragments arranged by size:
1498 1474 1330 1255 1251 445 185 154
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3795 3930 6008
Size: 274 80 33 51 1443 1818 135 2078
Cuts at: 6008 7720 96
Size: 1712 138
Fragments arranged by size:
2078 1818 1712 1443 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 3356 3388 7722 7754 2536
Size: 32 788 32 4334 32 2544
Fragments arranged by size:
4334 2544 788 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7762
XbaI T'CTAG_A
Cuts at: 5098 5098
Size: 7762
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2734 2734
Size: 7762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7762
XmaI C'CCGG_G
Cuts at: 1870 5087 1870
Size: 3217 4545
XmnI GAAnn'nnTTC
Cuts at: 4100 5891 4100
Size: 1791 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2618 5570 276
Size: 53 83 186 936 1084 2952 2468
Fragments arranged by size:
2952 2468 1084 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BsiWI BsmBI
DraIII FseI FspAI NruI PacI PspXI PsrI RsrII
SacII SexAI SfiI SrfI SwaI