(Linear) (Six-base) MAPSORT of: KIEE6765.seq Check: 8780 from: 1 to: 3057 KIEE6765.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AatII G_ACGT'C Cuts at: 0 615 3057 Size: 615 2442 AccI GT'mk_AC Cuts at: 0 561 2186 3057 Size: 561 1625 871 Fragments arranged by size: 1625 871 561 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 909 1572 1874 2110 2305 3057 Size: 909 663 302 236 195 752 Fragments arranged by size: 909 752 663 302 236 195 AflIII A'CryG_T Cuts at: 0 266 3057 Size: 266 2791 AleI CACnn'nnGTG Cuts at: 0 2710 3057 Size: 2710 347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2267 3057 Size: 2267 790 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2929 2961 3057 Size: 2929 32 96 Fragments arranged by size: 2929 96 32 AlwNI CAG_nnn'CTG Cuts at: 0 91 2457 3057 Size: 91 2366 600 Fragments arranged by size: 2366 600 91 ApaI G_GGCC'C Cuts at: 0 1556 2919 3057 Size: 1556 1363 138 Fragments arranged by size: 1556 1363 138 ApoI r'AATT_y Cuts at: 0 685 982 2010 2101 3057 Size: 685 297 1028 91 956 Fragments arranged by size: 1028 956 685 297 91 AvaI C'yCGr_G Cuts at: 0 555 1518 3057 Size: 555 963 1539 Fragments arranged by size: 1539 963 555 BamHI G'GATC_C Cuts at: 0 2693 2741 3057 Size: 2693 48 316 Fragments arranged by size: 2693 316 48 BanI G'GyrC_C Cuts at: 0 452 816 1056 1780 1902 1997 3057 Size: 452 364 240 724 122 95 1060 Fragments arranged by size: 1060 724 452 364 240 122 95 BanII G_rGCy'C Cuts at: 0 302 419 544 1046 1143 1556 2360 2919 Size: 302 117 125 502 97 413 804 559 Cuts at: 2919 2942 3057 Size: 23 115 Fragments arranged by size: 804 559 502 413 302 125 117 115 97 23 BbeI G_GCGC'C Cuts at: 0 2001 3057 Size: 2001 1056 BbsI GAAGACnn'nnnn_ Cuts at: 0 584 2487 2511 2562 3057 Size: 584 1903 24 51 495 Fragments arranged by size: 1903 584 495 51 24 BciVI GTATCCnnnnn_n' Cuts at: 0 153 2554 3019 3057 Size: 153 2401 465 38 Fragments arranged by size: 2401 465 153 38 BfrBI ATG'CAT Cuts at: 0 1251 3057 Size: 1251 1806 BglI GCCn_nnn'nGGC Cuts at: 0 1900 2133 3057 Size: 1900 233 924 Fragments arranged by size: 1900 924 233 BglII A'GATC_T Cuts at: 0 960 3031 3057 Size: 960 2071 26 Fragments arranged by size: 2071 960 26 BlpI GC'TnA_GC Cuts at: 0 92 1966 3057 Size: 92 1874 1091 Fragments arranged by size: 1874 1091 92 Bme1580I G_kGCm'C Cuts at: 0 1061 1486 1556 2436 2919 3057 Size: 1061 425 70 880 483 138 Fragments arranged by size: 1061 880 483 425 138 70 BmgBI CAC'GTC Cuts at: 0 933 3057 Size: 933 2124 BmrI ACTGGGnnnn_n' Cuts at: 0 859 2445 2937 3057 Size: 859 1586 492 120 Fragments arranged by size: 1586 859 492 120 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1743 2590 3057 Size: 1743 847 467 Fragments arranged by size: 1743 847 467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 273 1034 1166 3057 Size: 85 188 761 132 1891 Fragments arranged by size: 1891 761 188 132 85 Bpu10I CC'TnA_GC Cuts at: 0 489 3057 Size: 489 2568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2395 3057 Size: 2395 662 BsaI GGTCTCn'nnnn_ Cuts at: 0 173 406 2419 3057 Size: 173 233 2013 638 Fragments arranged by size: 2013 638 233 173 BsaAI yAC'GTr Cuts at: 0 267 2370 2679 3057 Size: 267 2103 309 378 Fragments arranged by size: 2103 378 309 267 BsaHI Gr'CG_yC Cuts at: 0 612 1998 3057 Size: 612 1386 1059 Fragments arranged by size: 1386 1059 612 BsaWI w'CCGG_w Cuts at: 0 66 1034 2267 3057 Size: 66 968 1233 790 Fragments arranged by size: 1233 968 790 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 334 364 531 561 2929 2959 3057 Size: 334 30 167 30 2368 30 98 Fragments arranged by size: 2368 334 167 98 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 72 462 818 1142 1163 1567 1732 Size: 28 44 390 356 324 21 404 165 Cuts at: 1732 2942 2950 3057 Size: 1210 8 107 Fragments arranged by size: 1210 404 390 356 324 165 107 44 28 21 8 BseYI C'CCAG_C Cuts at: 0 22 84 798 1101 2190 2831 3057 Size: 22 62 714 303 1089 641 226 Fragments arranged by size: 1089 714 641 303 226 62 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 2734 3057 Size: 268 2466 323 Fragments arranged by size: 2466 323 268 BsiHKAI G_wGCw'C Cuts at: 0 302 419 731 1143 2347 2738 3057 Size: 302 117 312 412 1204 391 319 Fragments arranged by size: 1204 412 391 319 312 302 117 Bsp1286I G_dGCh'C Cuts at: 0 302 419 544 731 1046 1061 1143 1486 Size: 302 117 125 187 315 15 82 343 Cuts at: 1486 1556 2347 2360 2436 2738 2919 2942 3057 Size: 70 791 13 76 302 181 23 115 Fragments arranged by size: 791 343 315 302 302 187 181 125 117 115 82 76 70 23 15 13 BspEI T'CCGG_A Cuts at: 0 66 1034 3057 Size: 66 968 2023 Fragments arranged by size: 2023 968 66 BspHI T'CATG_A Cuts at: 0 689 2780 3057 Size: 689 2091 277 Fragments arranged by size: 2091 689 277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 316 590 3057 Size: 316 274 2467 Fragments arranged by size: 2467 316 274 BsrBI CCG'CTC Cuts at: 0 485 3057 Size: 485 2572 BsrDI GCAATG_nn' Cuts at: 0 896 1161 1605 3017 3057 Size: 896 265 444 1412 40 Fragments arranged by size: 1412 896 444 265 40 BsrFI r'CCGG_y Cuts at: 0 1207 2296 2482 2553 3057 Size: 1207 1089 186 71 504 Fragments arranged by size: 1207 1089 504 186 71 BsrGI T'GTAC_A Cuts at: 0 1925 3057 Size: 1925 1132 BssSI C'ACGA_G Cuts at: 0 730 2920 3057 Size: 730 2190 137 Fragments arranged by size: 2190 730 137 BstBI TT'CG_AA Cuts at: 0 2805 3057 Size: 2805 252 BstEII G'GTnAC_C Cuts at: 0 2217 3057 Size: 2217 840 BstXI CCAn_nnnn'nTGG Cuts at: 0 1114 3057 Size: 1114 1943 BstYI r'GATC_y Cuts at: 0 698 960 2693 2717 2741 2994 3031 3057 Size: 698 262 1733 24 24 253 37 26 Fragments arranged by size: 1733 698 262 253 37 26 24 24 BstZ17I GTA'TAC Cuts at: 0 2187 3057 Size: 2187 870 Bsu36I CC'TnA_GG Cuts at: 0 445 3057 Size: 445 2612 BtgI C'CryG_G Cuts at: 0 572 1490 2176 2378 2753 2975 3057 Size: 572 918 686 202 375 222 82 Fragments arranged by size: 918 686 572 375 222 202 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 750 2376 3057 Size: 750 1626 681 Fragments arranged by size: 1626 750 681 BtsI GCAGTG_nn' Cuts at: 0 1185 1203 1484 2249 3012 3057 Size: 1185 18 281 765 763 45 Fragments arranged by size: 1185 765 763 281 45 18 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1143 1178 3057 Size: 1143 35 1879 Fragments arranged by size: 1879 1143 35 DrdI GACnn_nn'nnGTC Cuts at: 0 617 3057 Size: 617 2440 EaeI y'GGCC_r Cuts at: 0 409 791 888 1670 3057 Size: 409 382 97 782 1387 Fragments arranged by size: 1387 782 409 382 97 EarI CTCTTCn'nnn_ Cuts at: 0 298 928 1519 1585 2635 2707 2890 2896 Size: 298 630 591 66 1050 72 183 6 Cuts at: 2896 3057 Size: 161 Fragments arranged by size: 1050 630 591 298 183 161 72 66 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1151 1226 1612 3057 Size: 1151 75 386 1445 Fragments arranged by size: 1445 1151 386 75 EcoICRI GAG'CTC Cuts at: 0 300 417 1141 3057 Size: 300 117 724 1916 Fragments arranged by size: 1916 724 300 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 273 909 1034 1166 1572 1874 2110 Size: 85 188 636 125 132 406 302 236 Cuts at: 2110 2305 3057 Size: 195 752 Fragments arranged by size: 752 636 406 302 236 195 188 132 125 85 EcoNI CCTnn'n_nnAGG Cuts at: 0 115 1148 2141 3057 Size: 115 1033 993 916 Fragments arranged by size: 1033 993 916 115 EcoO109I rG'GnC_Cy Cuts at: 0 80 853 1023 1552 2915 3057 Size: 80 773 170 529 1363 142 Fragments arranged by size: 1363 773 529 170 142 80 EcoRI G'AATT_C Cuts at: 0 2010 3057 Size: 2010 1047 EcoRV GAT'ATC Cuts at: 0 1311 3057 Size: 1311 1746 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1596 1839 3057 Size: 1596 243 1218 Fragments arranged by size: 1596 1218 243 HaeII r_GCGC'y Cuts at: 0 2001 2041 3057 Size: 2001 40 1016 Fragments arranged by size: 2001 1016 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 147 179 334 366 1514 1546 3057 Size: 147 32 155 32 1148 32 1511 Fragments arranged by size: 1511 1148 155 147 32 32 32 HincII GTy'rAC Cuts at: 0 2265 3057 Size: 2265 792 KasI G'GCGC_C Cuts at: 0 1997 3057 Size: 1997 1060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 746 1931 3022 3057 Size: 746 1185 1091 35 Fragments arranged by size: 1185 1091 746 35 MscI TGG'CCA Cuts at: 0 1672 3057 Size: 1672 1385 MslI CAynn'nnrTG Cuts at: 0 1489 2332 2584 2710 3057 Size: 1489 843 252 126 347 Fragments arranged by size: 1489 843 347 252 126 MspA1I CmG'CkG Cuts at: 0 88 675 1223 1648 2685 3057 Size: 88 587 548 425 1037 372 Fragments arranged by size: 1037 587 548 425 372 88 NaeI GCC'GGC Cuts at: 0 1209 2298 3057 Size: 1209 1089 759 Fragments arranged by size: 1209 1089 759 NarI GG'CG_CC Cuts at: 0 1998 3057 Size: 1998 1059 NcoI C'CATG_G Cuts at: 0 1490 2975 3057 Size: 1490 1485 82 Fragments arranged by size: 1490 1485 82 NgoMIV G'CCGG_C Cuts at: 0 1207 2296 3057 Size: 1207 1089 761 Fragments arranged by size: 1207 1089 761 NsiI A_TGCA'T Cuts at: 0 1253 3057 Size: 1253 1804 PasI CC'CwG_GG Cuts at: 0 2437 3057 Size: 2437 620 PflMI CCAn_nnn'nTGG Cuts at: 0 2457 3057 Size: 2457 600 PfoI T'CCnGG_A Cuts at: 0 101 419 1011 1143 3057 Size: 101 318 592 132 1914 Fragments arranged by size: 1914 592 318 132 101 PmlI CAC'GTG Cuts at: 0 267 2679 3057 Size: 267 2412 378 Fragments arranged by size: 2412 378 267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2929 2961 3057 Size: 2929 32 96 Fragments arranged by size: 2929 96 32 PpuMI rG'GwC_Cy Cuts at: 0 80 853 1023 3057 Size: 80 773 170 2034 Fragments arranged by size: 2034 773 170 80 PsiI TTA'TAA Cuts at: 0 2965 3057 Size: 2965 92 PspOMI G'GGCC_C Cuts at: 0 1552 2915 3057 Size: 1552 1363 142 Fragments arranged by size: 1552 1363 142 PstI C_TGCA'G Cuts at: 0 170 317 3057 Size: 170 147 2740 Fragments arranged by size: 2740 170 147 PvuII CAG'CTG Cuts at: 0 88 1648 3057 Size: 88 1560 1409 Fragments arranged by size: 1560 1409 88 SacI G_AGCT'C Cuts at: 0 302 419 1143 3057 Size: 302 117 724 1914 Fragments arranged by size: 1914 724 302 117 SanDI GG'GwC_CC Cuts at: 0 80 1023 3057 Size: 80 943 2034 Fragments arranged by size: 2034 943 80 SapI GCTCTTCn'nnn_ Cuts at: 0 2635 3057 Size: 2635 422 ScaI AGT'ACT Cuts at: 0 1792 3057 Size: 1792 1265 SfcI C'TryA_G Cuts at: 0 166 313 3057 Size: 166 147 2744 Fragments arranged by size: 2744 166 147 SfoI GGC'GCC Cuts at: 0 1999 3057 Size: 1999 1058 SmlI C'TyrA_G Cuts at: 0 2410 3057 Size: 2410 647 StyI C'CwwG_G Cuts at: 0 704 1490 2975 3057 Size: 704 786 1485 82 Fragments arranged by size: 1485 786 704 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 826 2156 2310 3057 Size: 826 1330 154 747 Fragments arranged by size: 1330 826 747 154 TatI w'GTAC_w Cuts at: 0 1790 1925 3057 Size: 1790 135 1132 Fragments arranged by size: 1790 1132 135 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 531 563 1351 1383 3057 Size: 531 32 788 32 1674 Fragments arranged by size: 1674 788 531 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 729 3057 Size: 729 2328 XmnI GAAnn'nnTTC Cuts at: 0 2095 3057 Size: 2095 962 ZraI GAC'GTC Cuts at: 0 613 3057 Size: 613 2444 Enzymes that do cut: AatII AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SanDI SapI ScaI SfcI SfoI SmlI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI BmtI BsaBI BsiEI BsiWI BsmI BsmBI BssHII BstAPI ClaI DraI DraIII EagI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NspI PacI PciI PmeI PshAI PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI