################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MslI MspI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 79 GTAGAC GT^[AC][GT]AC AciI 616 CCGC CCGC(-3/-1) AcsI 327 AAATTT [GA]^AATT[CT] AfaI 69 GTAC GT^AC Alw44I 357 GTGCAC G^TGCAC ApaLI 357 GTGCAC G^TGCAC ApoI 327 AAATTT [GA]^AATT[CT] AspHI 357 GTGCAC G[AT]GC[AT]^C BbsI 751 GAAGAC GAAGAC(2/6) BfaI 586 CTAG C^TAG BglII 405 AGATCT A^GATCT BmyI 357 GTGCAC G[AGT]GC[ACT]^C BpuAI 751 GAAGAC GAAGAC(2/6) BsgI 5 GTGCAG GTGCAG(16/14) BsiHKAI 357 GTGCAC G[AT]GC[AT]^C Bsp1286I 357 GTGCAC G[AGT]GC[ACT]^C BsrDI 157 GCAATG GCAATG(2/0) BstAPI 7 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 405 AGATCT [GA]^GATC[CT] Cac8I 96 GCGTGC GC[ACGT]^[ACGT]GC FauI 615 CCCGC CCCGC(4/6) HindIII 724 AAGCTT A^AGCTT MaeI 586 CTAG C^TAG MaeIII 317 GTGAC ^GT[ACGT]AC MflI 405 AGATCT [GA]^GATC[CT] MspA1I 394 CAGCTG C[AC]G^C[GT]G MwoI 7 GCAGCTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 40 CATATG CA^TATG PstI 742 CTGCAG CTGCA^G PvuII 394 CAGCTG CAG^CTG RsaI 69 GTAC GT^AC SfaNI 423 GCATC GCATC(5/9) SfcI 742 CTGCAG C^T[GA][CT]AG Tsp45I 317 GTGAC ^GT[GC]AC XbaI 585 TCTAGA T^CTAGA XhoII 405 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BsmI 652 GAATGC GAATGC(1/-1) 438 GCATTC BsrI 569 ACTGG ACTGG(1/-1) 76 CCAGT EarI 289 GAAGAG CTCTTC(1/4) 481 GAAGAG HincII 162 GTTGAC GT[CT]^[GA]AC 487 GTTGAC HindII 162 GTTGAC GT[CT]^[GA]AC 487 GTTGAC HinfI 262 GAATC G^A[ACGT]TC 582 GATTC Ksp632I 289 GAAGAG CTCTTC(1/4) 481 GAAGAG MfeI 91 CAATTG C^AATTG 505 CAATTG MunI 91 CAATTG C^AATTG 505 CAATTG TfiI 262 GAATC G^A[AT]TC 582 GATTC TspRI 355 CAGTG CA[GC]TG[ACGT][ACGT]^ 687 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI AlwNI BbvI BpmI DdeI DpnII Fnu4HI ItaI MboI MboII MnlI MseI NdeII Sau3AI Tru9I TseI Tsp509I