(Circular) (Six-base) MAPSORT of: pFN21AE6658.seq Check: 1232 from: 1 to: 5470 pFN21AE6658 5470 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3280 278 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2085 2813 1937 Size: 148 728 4594 Fragments arranged by size: 4594 728 148 Acc65I G'GTAC_C Cuts at: 2791 2791 Size: 5470 AclI AA'CG_TT Cuts at: 1563 3597 3970 1563 Size: 2034 373 3063 Fragments arranged by size: 3063 2034 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2133 3533 4917 5185 1632 Size: 501 1400 1384 268 1917 Fragments arranged by size: 1917 1400 1384 501 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5273 AflIII A'CryG_T Cuts at: 1203 4369 5346 1203 Size: 3166 977 1327 Fragments arranged by size: 3166 1327 977 AgeI A'CCGG_T Cuts at: 4301 4301 Size: 5470 AhdI GACnn_n'nnGTC Cuts at: 4199 4199 Size: 5470 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5470 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5470 AlwNI CAG_nnn'CTG Cuts at: 2018 2242 2401 3213 4785 2018 Size: 224 159 812 1572 2703 Fragments arranged by size: 2703 1572 812 224 159 ApaLI G'TGCA_C Cuts at: 2362 3527 4683 2362 Size: 1165 1156 3149 Fragments arranged by size: 3149 1165 1156 ApoI r'AATT_y Cuts at: 1493 2332 2779 3005 3157 5266 1493 Size: 839 447 226 152 2109 1697 Fragments arranged by size: 2109 1697 839 447 226 152 AseI AT'TA_AT Cuts at: 160 1032 4024 160 Size: 872 2992 1606 Fragments arranged by size: 2992 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 AvaI C'yCGr_G Cuts at: 1870 1943 2795 1870 Size: 73 852 4545 Fragments arranged by size: 4545 852 73 BamHI G'GATC_C Cuts at: 2800 3167 2800 Size: 367 5103 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 4246 618 Size: 325 217 627 1004 1455 1842 Fragments arranged by size: 1842 1455 1004 627 325 217 BanII G_rGCy'C Cuts at: 1391 2789 1391 Size: 1398 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2763 928 Size: 971 864 3635 Fragments arranged by size: 3635 971 864 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3661 3695 1903 Size: 34 1724 34 3678 Fragments arranged by size: 3678 1724 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3362 4572 3362 Size: 1210 4260 BclI T'GATC_A Cuts at: 1548 1548 Size: 5470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4081 136 Size: 107 122 71 631 605 2409 1525 Fragments arranged by size: 2409 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2410 5465 2410 Size: 3055 2415 BlpI GC'TnA_GC Cuts at: 2887 2887 Size: 5470 Bme1580I G_kGCm'C Cuts at: 1790 2366 3531 4687 1790 Size: 576 1165 1156 2573 Fragments arranged by size: 2573 1165 1156 576 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3201 4159 442 Size: 416 53 83 399 24 1784 958 1753 Fragments arranged by size: 1784 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5470 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 5470 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2010 2561 2596 4130 1239 Size: 771 551 35 1534 2579 Fragments arranged by size: 2579 1534 771 551 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3595 4460 4758 3595 Size: 865 298 4307 Fragments arranged by size: 4307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4133 882 Size: 617 2634 2219 Fragments arranged by size: 2634 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3261 493 Size: 711 2057 2702 Fragments arranged by size: 2702 2057 711 BsaBI GATnn'nnATC Cuts at: 5464 5464 Size: 5470 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3277 3659 4294 275 Size: 53 83 186 936 1744 382 635 1451 Fragments arranged by size: 1744 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3903 4301 4575 4722 5133 3903 Size: 398 274 147 411 4240 Fragments arranged by size: 4240 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5245 Fragments arranged by size: 5245 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5470 BseYI C'CCAG_C Cuts at: 4673 5169 4673 Size: 496 4974 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2031 1163 Size: 573 295 4602 Fragments arranged by size: 4602 573 295 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2920 3681 4709 1540 Size: 275 186 919 761 1028 2301 Fragments arranged by size: 2301 1028 919 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2366 3531 3616 4687 2366 Size: 1165 85 1071 3149 Fragments arranged by size: 3149 1165 1071 85 BsmI GAATG_Cn' Cuts at: 2443 2663 2981 3074 2443 Size: 220 318 93 4839 Fragments arranged by size: 4839 318 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2366 2789 3531 3616 4687 1391 Size: 399 576 423 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 576 423 399 85 BspEI T'CCGG_A Cuts at: 5133 5133 Size: 5470 BspHI T'CATG_A Cuts at: 3359 5089 3359 Size: 1730 3740 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2825 844 Size: 1981 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3357 1394 Size: 429 1534 3507 Fragments arranged by size: 3507 1534 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 3965 4139 65 Size: 1183 921 1796 174 1396 Fragments arranged by size: 1796 1396 1183 921 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4114 4301 1700 Size: 110 2304 187 2869 Fragments arranged by size: 2869 2304 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5470 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5470 BssSI C'ACGA_G Cuts at: 3530 4542 3530 Size: 1012 4458 BstAPI GCAn_nnn'nTGC Cuts at: 794 2018 794 Size: 1224 4246 BstBI TT'CG_AA Cuts at: 4274 4274 Size: 5470 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5470 BstYI r'GATC_y Cuts at: 1972 2410 2800 3167 3552 3569 5021 5107 5119 Size: 438 390 367 385 17 1452 86 12 Cuts at: 5119 5465 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 438 390 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4918 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4731 Fragments arranged by size: 4731 683 56 BtsI GCAGTG_nn' Cuts at: 2986 3780 3800 2986 Size: 794 20 4656 Fragments arranged by size: 4656 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5470 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2204 2239 529 Size: 35 1640 35 3760 Fragments arranged by size: 3760 1640 35 35 DraI TTT'AAA Cuts at: 2774 2774 Size: 5470 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4477 809 Size: 507 3161 1802 Fragments arranged by size: 3161 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2917 3806 5435 Size: 54 1425 50 275 18 1087 889 1629 Cuts at: 5435 8 Size: 43 Fragments arranged by size: 1629 1425 1087 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2917 1537 Size: 275 1105 4090 Fragments arranged by size: 4090 1105 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2481 3400 1353 Size: 936 192 919 3423 Fragments arranged by size: 3423 936 919 192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4057 4431 4577 387 Size: 1064 138 2468 374 146 1280 Fragments arranged by size: 2468 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2010 2133 2561 2596 3533 4130 4917 Size: 393 378 123 428 35 937 597 787 Cuts at: 4917 5185 1239 Size: 268 1524 Fragments arranged by size: 1524 937 787 597 428 393 378 268 123 35 EcoRI G'AATT_C Cuts at: 2779 3157 2779 Size: 378 5092 FspI TGC'GCA Cuts at: 3976 3976 Size: 5470 HaeII r_GCGC'y Cuts at: 1678 4617 1678 Size: 2939 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2395 2427 2578 2610 2663 Size: 32 135 32 823 32 151 32 53 Cuts at: 2663 2695 4111 4143 4185 4217 1373 Size: 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 823 151 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2169 2494 2814 3060 669 Size: 988 281 231 325 320 246 3079 Fragments arranged by size: 3079 988 325 320 281 246 231 HindIII A'AGCT_T Cuts at: 748 2729 748 Size: 1981 3489 HpaI GTT'AAC Cuts at: 3060 3060 Size: 5470 KpnI G_GTAC'C Cuts at: 2795 2795 Size: 5470 MfeI C'AATT_G Cuts at: 2096 2510 3069 2096 Size: 414 559 4497 Fragments arranged by size: 4497 559 414 MluI A'CGCG_T Cuts at: 5346 5346 Size: 5470 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 4584 4768 1305 Size: 1428 1851 184 2007 Fragments arranged by size: 2007 1851 1428 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3648 Fragments arranged by size: 3648 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3428 3787 3946 518 Size: 648 2262 359 159 2042 Fragments arranged by size: 2262 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2401 2886 3563 4711 4956 1606 Size: 795 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 795 677 485 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4918 NdeI CA'TA_TG Cuts at: 387 2046 387 Size: 1659 3811 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5470 NotI GC'GGCC_GC Cuts at: 2917 2917 Size: 5470 NspI r_CATG'y Cuts at: 1132 1229 2828 4373 1132 Size: 97 1599 1545 2229 Fragments arranged by size: 2229 1599 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5470 PciI A'CATG_T Cuts at: 4369 4369 Size: 5470 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5470 PmeI GTTT'AAAC Cuts at: 2774 2774 Size: 5470 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3539 3571 5052 5084 3539 Size: 32 1481 32 3925 Fragments arranged by size: 3925 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5470 PsiI TTA'TAA Cuts at: 3040 3040 Size: 5470 PstI C_TGCA'G Cuts at: 830 2751 2822 830 Size: 1921 71 3478 Fragments arranged by size: 3478 1921 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5470 PvuII CAG'CTG Cuts at: 2401 2401 Size: 5470 SalI G'TCGA_C Cuts at: 1936 2812 1936 Size: 876 4594 SbfI CC_TGCA'GG Cuts at: 2822 2822 Size: 5470 ScaI AGT'ACT Cuts at: 3718 5430 3718 Size: 1712 3758 SfcI C'TryA_G Cuts at: 826 1045 2747 2818 3953 4634 4825 826 Size: 219 1702 71 1135 681 191 1471 Fragments arranged by size: 1702 1471 1135 681 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5470 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5470 SmaI CCC'GGG Cuts at: 1872 2797 1872 Size: 925 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3574 4475 4737 820 Size: 197 926 1631 901 262 1553 Fragments arranged by size: 1631 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3261 493 Size: 2768 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5470 SphI G_CATG'C Cuts at: 1132 2828 1132 Size: 1696 3774 SspI AAT'ATT Cuts at: 5 52 3394 5 Size: 47 3342 2081 Fragments arranged by size: 3342 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5470 StyI C'CwwG_G Cuts at: 513 1065 2909 513 Size: 552 1844 3074 Fragments arranged by size: 3074 1844 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2466 3497 3514 3667 3852 4297 325 Size: 1255 886 1031 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1031 886 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 3716 5428 Size: 274 80 33 51 1443 96 1643 1712 Cuts at: 5428 96 Size: 138 Fragments arranged by size: 1712 1643 1443 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5430 5462 5430 Size: 32 5438 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5470 XbaI T'CTAG_A Cuts at: 2590 2806 2590 Size: 216 5254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5470 XmaI C'CCGG_G Cuts at: 1870 2795 1870 Size: 925 4545 XmnI GAAnn'nnTTC Cuts at: 3599 3599 Size: 5470 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3278 276 Size: 53 83 186 936 1744 2468 Fragments arranged by size: 2468 1744 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI