Restriction map for
pFN21AE5177
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# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MboI MflI MluI MluNI
MroI MscI MspA1I MspI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 365 AAATTC [GA]^AATT[CT]
AfaI 255 GTAC GT^AC
AluI 71 AGCT AG^CT
ApoI 365 AAATTC [GA]^AATT[CT]
AspI 417 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 339 GGCACC G^G[CT][GA]CC
BpmI 55 CTCCAG CTGGAG(16/14)
BsaJI 437 CCCAGG C^C[ACGT][ACGT]GG
BsiYI 243 CCTGAGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 243 CCTGAGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 373 GAGAC GTCTC(1/5)
BsrDI 270 GCAATG GCAATG(2/0)
BsrI 57 CCAGT ACTGG(1/-1)
BstEII 49 GGTTACC G^GT[ACGT]ACC
BstPI 49 GGTTACC G^GT[ACGT]ACC
Cac8I 327 GCATGC GC[ACGT]^[ACGT]GC
DraII 310 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 243 CCTGAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 310 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 49 GGTTACC G^GT[ACGT]ACC
HaeIII 435 GGCC GG^CC
HphI 9 TCACC GGTGA(8/7)
MfeI 386 CAATTG C^AATTG
MunI 386 CAATTG C^AATTG
NlaIII 328 CATG CATG^
NlaIV 339 GGCACC GG[ACGT]^[ACGT]CC
NspI 327 GCATGC [GA]CATG^[CT]
PflFI 417 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 421 GAGTC GAGTC(4/5)
PpuMI 310 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 277 CTGCAG CTGCA^G
RsaI 255 GTAC GT^AC
SfaNI 201 GATGC GCATC(5/9)
SmlI 265 CTTGAG C^T[CT][GA]AG
SphI 327 GCATGC GCATG^C
TspRI 58 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 417 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 170 GGTCC G^G[AT]CC
311 GGTCC
BsmI 446 GAATGC GAATGC(1/-1)
143 GCATTC
BssKI 173 CCAGG ^CC[ACGT]GG
438 CCAGG
BstAPI 270 GCAATGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
340 GCACCTCTTGC
BstF5I 34 CATCC GGATG(2/0)
66 CATCC
BstNI 173 CCAGG CC^[AT]GG
438 CCAGG
DdeI 244 CTGAG C^T[ACGT]AG
332 CTGAG
EcoRII 173 CCAGG ^CC[AT]GG
438 CCAGG
FokI 34 CATCC GGATG(9/13)
66 CATCC
MboII 391 GAAGA GAAGA(8/7)
147 TCTTC
MseI 44 TTAA T^TAA
157 TTAA
MslI 10 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
195 CACCAAGATG
MvaI 173 CCAGG CC^[AT]GG
438 CCAGG
ScrFI 173 CCAGG CC^[ACGT]GG
438 CCAGG
SfcI 223 CTACAG C^T[GA][CT]AG
277 CTGCAG
TfiI 3 GAATC G^A[AT]TC
400 GAATC
Tru9I 44 TTAA T^TAA
157 TTAA
Tsp45I 8 GTCAC ^GT[GC]AC
62 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI Cfr13I Fnu4HI HinfI ItaI
MaeIII MnlI MwoI Sau96I TseI
Tsp509I