(Circular) (Six-base) MAPSORT of: pFN21AE5177.seq Check: 6516 from: 1 to: 5158 pFN21AE5177 5158 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2968 278 Size: 53 83 186 936 1432 2468 Fragments arranged by size: 2468 1432 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2501 1937 Size: 564 4594 Acc65I G'GTAC_C Cuts at: 2479 2479 Size: 5158 AclI AA'CG_TT Cuts at: 1563 3285 3658 1563 Size: 1722 373 3063 Fragments arranged by size: 3063 1722 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3221 4605 4873 1632 Size: 1589 1384 268 1917 Fragments arranged by size: 1917 1589 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4961 AflIII A'CryG_T Cuts at: 1203 4057 5034 1203 Size: 2854 977 1327 Fragments arranged by size: 2854 1327 977 AgeI A'CCGG_T Cuts at: 3989 3989 Size: 5158 AhdI GACnn_n'nnGTC Cuts at: 3887 3887 Size: 5158 AleI CACnn'nnGTG Cuts at: 1166 2019 1166 Size: 853 4305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5158 AlwNI CAG_nnn'CTG Cuts at: 2901 4473 2901 Size: 1572 3586 ApaLI G'TGCA_C Cuts at: 3215 4371 3215 Size: 1156 4002 ApoI r'AATT_y Cuts at: 1493 2370 2467 2693 2845 4954 1493 Size: 877 97 226 152 2109 1697 Fragments arranged by size: 2109 1697 877 226 152 97 AseI AT'TA_AT Cuts at: 160 1032 3712 160 Size: 872 2680 1606 Fragments arranged by size: 2680 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5158 AvaI C'yCGr_G Cuts at: 1870 1943 2483 1870 Size: 73 540 4545 Fragments arranged by size: 4545 540 73 BamHI G'GATC_C Cuts at: 2488 2855 2488 Size: 367 4791 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2344 2479 3934 618 Size: 325 217 627 557 135 1455 1842 Fragments arranged by size: 1842 1455 627 557 325 217 135 BanII G_rGCy'C Cuts at: 1391 2477 1391 Size: 1086 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3349 3383 1903 Size: 34 1412 34 3678 Fragments arranged by size: 3678 1412 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3050 4260 3050 Size: 1210 3948 BclI T'GATC_A Cuts at: 1548 1548 Size: 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3769 136 Size: 107 122 71 631 605 2097 1525 Fragments arranged by size: 2097 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5153 5153 Size: 5158 BlpI GC'TnA_GC Cuts at: 2575 2575 Size: 5158 Bme1580I G_kGCm'C Cuts at: 1790 3219 4375 1790 Size: 1429 1156 2573 Fragments arranged by size: 2573 1429 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5158 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2889 3847 442 Size: 416 53 83 399 24 1472 958 1753 Fragments arranged by size: 1753 1472 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5158 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 5158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 3818 1239 Size: 806 1773 2579 Fragments arranged by size: 2579 1773 806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 3283 4148 4446 2291 Size: 992 865 298 3003 Fragments arranged by size: 3003 992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3821 882 Size: 617 2322 2219 Fragments arranged by size: 2322 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2949 493 Size: 711 1745 2702 Fragments arranged by size: 2702 1745 711 BsaBI GATnn'nnATC Cuts at: 5152 5152 Size: 5158 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2965 3347 3982 275 Size: 53 83 186 936 1432 382 635 1451 Fragments arranged by size: 1451 1432 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3591 3989 4263 4410 4821 3591 Size: 398 274 147 411 3928 Fragments arranged by size: 3928 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4933 Fragments arranged by size: 4933 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5158 BseYI C'CCAG_C Cuts at: 4361 4857 4361 Size: 496 4662 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4585 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2608 3369 4397 1540 Size: 275 186 607 761 1028 2301 Fragments arranged by size: 2301 1028 761 607 275 186 BsiHKAI G_wGCw'C Cuts at: 3219 3304 4375 3219 Size: 85 1071 4002 Fragments arranged by size: 4002 1071 85 BsmI GAATG_Cn' Cuts at: 2148 2457 2669 2762 2148 Size: 309 212 93 4544 Fragments arranged by size: 4544 309 212 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2477 3219 3304 4375 1391 Size: 399 687 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 687 399 85 BspEI T'CCGG_A Cuts at: 4821 4821 Size: 5158 BspHI T'CATG_A Cuts at: 3047 4777 3047 Size: 1730 3428 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 2513 844 Size: 1602 67 3489 Fragments arranged by size: 3489 1602 67 BsrBI CCG'CTC Cuts at: 1394 1823 3045 1394 Size: 429 1222 3507 Fragments arranged by size: 3507 1222 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2282 3653 3827 65 Size: 1183 1034 1371 174 1396 Fragments arranged by size: 1396 1371 1183 1034 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3802 3989 1700 Size: 110 1992 187 2869 Fragments arranged by size: 2869 1992 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5158 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5158 BssSI C'ACGA_G Cuts at: 3218 4230 3218 Size: 1012 4146 BstAPI GCAn_nnn'nTGC Cuts at: 794 2281 2351 794 Size: 1487 70 3601 Fragments arranged by size: 3601 1487 70 BstBI TT'CG_AA Cuts at: 3962 3962 Size: 5158 BstEII G'GTnAC_C Cuts at: 1184 2054 1184 Size: 870 4288 BstYI r'GATC_y Cuts at: 1972 2488 2855 3240 3257 4709 4795 4807 5153 Size: 516 367 385 17 1452 86 12 346 Cuts at: 5153 1972 Size: 1977 Fragments arranged by size: 1977 1452 516 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4606 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4419 Fragments arranged by size: 4419 683 56 BtsI GCAGTG_nn' Cuts at: 2674 3468 3488 2674 Size: 794 20 4344 Fragments arranged by size: 4344 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5123 DraI TTT'AAA Cuts at: 2462 2462 Size: 5158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4165 809 Size: 507 2849 1802 Fragments arranged by size: 2849 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2605 3494 5123 Size: 54 1425 50 275 18 775 889 1629 Cuts at: 5123 8 Size: 43 Fragments arranged by size: 1629 1425 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2605 1537 Size: 275 793 4090 Fragments arranged by size: 4090 793 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3088 1353 Size: 1735 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3745 4119 4265 387 Size: 1064 138 2156 374 146 1280 Fragments arranged by size: 2156 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 3221 3818 4605 4873 1239 Size: 393 413 1176 597 787 268 1524 Fragments arranged by size: 1524 1176 787 597 413 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2252 Size: 5158 EcoO109I rG'GnC_Cy Cuts at: 2316 2316 Size: 5158 EcoRI G'AATT_C Cuts at: 2467 2845 2467 Size: 378 4780 FspI TGC'GCA Cuts at: 3664 3664 Size: 5158 HaeII r_GCGC'y Cuts at: 1678 4305 1678 Size: 2627 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3799 3831 3873 3905 1373 Size: 32 135 32 2227 32 42 32 2626 Fragments arranged by size: 2626 2227 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2502 2748 669 Size: 988 281 564 246 3079 Fragments arranged by size: 3079 988 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5158 HpaI GTT'AAC Cuts at: 2748 2748 Size: 5158 KpnI G_GTAC'C Cuts at: 2483 2483 Size: 5158 MfeI C'AATT_G Cuts at: 2391 2757 2391 Size: 366 4792 MluI A'CGCG_T Cuts at: 5034 5034 Size: 5158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4272 4456 1305 Size: 2967 184 2007 Fragments arranged by size: 2967 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3336 Fragments arranged by size: 3336 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2019 2204 3116 3475 3634 518 Size: 648 853 185 912 359 159 2042 Fragments arranged by size: 2042 912 853 648 359 185 159 MspA1I CmG'CkG Cuts at: 1606 2574 3251 4399 4644 1606 Size: 968 677 1148 245 2120 Fragments arranged by size: 2120 1148 968 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4606 NdeI CA'TA_TG Cuts at: 387 387 Size: 5158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5158 NotI GC'GGCC_GC Cuts at: 2605 2605 Size: 5158 NspI r_CATG'y Cuts at: 1132 1229 2336 2516 4061 1132 Size: 97 1107 180 1545 2229 Fragments arranged by size: 2229 1545 1107 180 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5158 PciI A'CATG_T Cuts at: 4057 4057 Size: 5158 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5158 PfoI T'CCnGG_A Cuts at: 2177 2177 Size: 5158 PmeI GTTT'AAAC Cuts at: 2462 2462 Size: 5158 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3227 3259 4740 4772 3227 Size: 32 1481 32 3613 Fragments arranged by size: 3613 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2316 2316 Size: 5158 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5158 PsiI TTA'TAA Cuts at: 2728 2728 Size: 5158 PstI C_TGCA'G Cuts at: 830 2286 2510 830 Size: 1456 224 3478 Fragments arranged by size: 3478 1456 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5158 SalI G'TCGA_C Cuts at: 1936 2500 1936 Size: 564 4594 SbfI CC_TGCA'GG Cuts at: 2510 2510 Size: 5158 ScaI AGT'ACT Cuts at: 3406 5118 3406 Size: 1712 3446 SfcI C'TryA_G Cuts at: 826 1045 2228 2282 2506 3641 4322 4513 826 Size: 219 1183 54 224 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 681 224 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5158 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5158 SmaI CCC'GGG Cuts at: 1872 2485 1872 Size: 613 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 3262 4163 4425 820 Size: 197 926 327 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 327 262 197 SnaBI TAC'GTA Cuts at: 493 2949 493 Size: 2456 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5158 SphI G_CATG'C Cuts at: 1132 2336 2516 1132 Size: 1204 180 3774 Fragments arranged by size: 3774 1204 180 SspI AAT'ATT Cuts at: 5 52 3082 5 Size: 47 3030 2081 Fragments arranged by size: 3030 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5158 StyI C'CwwG_G Cuts at: 513 1065 2597 513 Size: 552 1532 3074 Fragments arranged by size: 3074 1532 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3185 3202 3355 3540 3985 325 Size: 1255 1605 17 153 185 445 1498 Fragments arranged by size: 1605 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3404 5116 96 Size: 274 80 33 51 1443 1427 1712 138 Fragments arranged by size: 1712 1443 1427 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5118 5150 5118 Size: 32 5126 Tth111I GACn'n_nGTC Cuts at: 1316 2425 1316 Size: 1109 4049 XbaI T'CTAG_A Cuts at: 2494 2494 Size: 5158 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5158 XmaI C'CCGG_G Cuts at: 1870 2483 1870 Size: 613 4545 XmnI GAAnn'nnTTC Cuts at: 3287 3287 Size: 5158 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2966 276 Size: 53 83 186 936 1432 2468 Fragments arranged by size: 2468 1432 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI