Restriction map for
pFN21AE5786
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BanI
BanII BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DsaI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 1905 CCGC CCGC(-3/-1)
AlwNI 97 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 574 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1568 GAGCAC G[AT]GC[AT]^C
AvaII 1054 GGTCC G^G[AT]CC
BamHI 207 GGATCC G^GATCC
BbrPI 526 CACGTG CAC^GTG
BpmI 1508 CTCCAG CTGGAG(16/14)
BsaAI 526 CACGTG [CT]AC^GT[GA]
BsiEI 1902 CGGCCG CG[GA][CT]^CG
BsiHKAI 1568 GAGCAC G[AT]GC[AT]^C
Bsp1407I 295 TGTACA T^GTACA
BspHI 396 TCATGA T^CATGA
BsrGI 295 TGTACA T^GTACA
BssKI 1689 CCAGG ^CC[ACGT]GG
BstAPI 898 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 375 TTCGAA TT^CGAA
BstNI 1689 CCAGG CC^[AT]GG
Cfr13I 1054 GGTCC G^G[ACGT]CC
DraI 1528 TTTAAA TTT^AAA
DrdI 1022 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1902 CGGCCG [CT]^GGCC[GA]
EagI 1902 CGGCCG C^GGCCG
EarI 1801 GAAGAG CTCTTC(1/4)
EclXI 1902 CGGCCG C^GGCCG
Eco52I 1902 CGGCCG C^GGCCG
EcoRI 1409 GAATTC G^AATTC
EcoRII 1689 CCAGG ^CC[AT]GG
EcoT22I 251 ATGCAT ATGCA^T
HphI 1997 TCACC GGTGA(8/7)
Ksp632I 1801 GAAGAG CTCTTC(1/4)
MslI 1594 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 460 CAGCTG C[AC]G^C[GT]G
MvaI 1689 CCAGG CC^[AT]GG
NlaIV 207 GGATCC GG[ACGT]^[ACGT]CC
NsiI 251 ATGCAT ATGCA^T
NspV 375 TTCGAA TT^CGAA
PaeR7I 794 CTCGAG C^TCGAG
PmaCI 526 CACGTG CAC^GTG
PmlI 526 CACGTG CAC^GTG
PvuII 460 CAGCTG CAG^CTG
RcaI 396 TCATGA T^CATGA
Sau96I 1054 GGTCC G^G[ACGT]CC
ScrFI 1689 CCAGG CC^[ACGT]GG
SfuI 375 TTCGAA TT^CGAA
SspBI 295 TGTACA T^GTACA
XbaI 1113 TCTAGA T^CTAGA
XhoI 794 CTCGAG C^TCGAG
XmnI 574 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 349 GTAGAC GT^[AC][GT]AC
557 GTCTAC
AvaI 794 CTCGAG C^[CT]CG[GA]G
1538 CCCGAG
BbsI 289 GAAGAC GAAGAC(2/6)
884 GTCTTC
BmyI 721 GTGCCC G[AGT]GC[ACT]^C
1568 GAGCAC
BpuAI 289 GAAGAC GAAGAC(2/6)
884 GTCTTC
BsiYI 1750 CCCCAAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2085 CCAATGTGGGG
BslI 1750 CCCCAAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2085 CCAATGTGGGG
BsmAI 595 GAGAC GTCTC(1/5)
969 GAGAC
BsoBI 794 CTCGAG C^[CT]CG[GA]G
1538 CCCGAG
Bsp1286I 721 GTGCCC G[AGT]GC[ACT]^C
1568 GAGCAC
BspMI 587 ACCTGC ACCTGC(4/8)
1785 GCAGGT
BsrDI 154 GCAATG GCAATG(2/0)
704 GCAATG
BsrI 943 ACTGG ACTGG(1/-1)
982 ACTGG
BstYI 207 GGATCC [GA]^GATC[CT]
1434 AGATCC
HaeIII 791 GGCC GG^CC
1903 GGCC
MaeII 527 ACGT A^CGT
566 ACGT
MfeI 1024 CAATTG C^AATTG
1727 CAATTG
MflI 207 GGATCC [GA]^GATC[CT]
1434 AGATCC
MunI 1024 CAATTG C^AATTG
1727 CAATTG
PstI 94 CTGCAG CTGCA^G
457 CTGCAG
XhoII 207 GGATCC [GA]^GATC[CT]
1434 AGATCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BsmFI BsmI
BstF5I DdeI DpnII EcoT14I Fnu4HI
FokI HinfI ItaI MaeI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NspI PleI RsaI
Sau3AI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I