Restriction map for
pFN21AE5786
(Circular) (Six-base) MAPSORT of: pFN21AE5786.seq Check: 975 from: 1 to: 6826
pFN21AE5786 6826 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 2563 4169 1937
Size: 418 208 1606 4594
Fragments arranged by size:
4594 1606 418 208
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2505 4889 6273 6541 1632
Size: 873 2384 1384 268 1917
Fragments arranged by size:
2384 1917 1384 873 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6826
AlwNI CAG_nnn'CTG
Cuts at: 2107 4569 6141 2107
Size: 2462 1572 2792
Fragments arranged by size:
2792 2462 1572
ApaLI G'TGCA_C
Cuts at: 4883 6039 4883
Size: 1156 5670
ApoI r'AATT_y
Cuts at: 1493 2328 2383 3414 3488 3744 3979 4135 4361
Size: 835 55 1031 74 256 235 156 226
Cuts at: 4361 4513 6622 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1031 835 256 235 226 156
152 74 55
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3543 4151 1870
Size: 73 856 744 608 4545
Fragments arranged by size:
4545 856 744 608 73
BamHI G'GATC_C
Cuts at: 2212 4156 4523 2212
Size: 1944 367 4515
Fragments arranged by size:
4515 1944 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4147 5602 618
Size: 325 217 627 2360 1455 1842
Fragments arranged by size:
2360 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4145 1391
Size: 2754 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 2882 928
Size: 971 402 581 4872
Fragments arranged by size:
4872 971 581 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4718 5928 4718
Size: 1210 5616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BfrBI ATG'CAT
Cuts at: 2258 2258
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5437 136
Size: 107 122 71 631 605 3765 1525
Fragments arranged by size:
3765 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6821 6821
Size: 6826
BlpI GC'TnA_GC
Cuts at: 4243 4243
Size: 6826
Bme1580I G_kGCm'C
Cuts at: 1790 2730 4887 6043 1790
Size: 940 2157 1156 2573
Fragments arranged by size:
2573 2157 1156 940
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4557 5515 442
Size: 416 53 83 399 24 3140 958 1753
Fragments arranged by size:
3140 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3498 5486 1239
Size: 2259 1988 2579
Fragments arranged by size:
2579 2259 1988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3048 3571 4951 5816 6114 2618
Size: 430 523 1380 865 298 3330
Fragments arranged by size:
3330 1380 865 523 430 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5489 882
Size: 617 3990 2219
Fragments arranged by size:
3990 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2533 4617 493
Size: 711 1329 2084 2702
Fragments arranged by size:
2702 2084 1329 711
BsaBI GATnn'nnATC
Cuts at: 6820 6820
Size: 6826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4633 5015 5650 275
Size: 53 83 186 936 3100 382 635 1451
Fragments arranged by size:
3100 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5259 5657 5931 6078 6489 5259
Size: 398 274 147 411 5596
Fragments arranged by size:
5596 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3673 3703 1178
Size: 30 165 30 2270 30 4301
Fragments arranged by size:
4301 2270 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6826
BseYI C'CCAG_C
Cuts at: 4058 6029 6525 4058
Size: 1971 496 4359
Fragments arranged by size:
4359 1971 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6253
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4276 5037 6065 1540
Size: 275 186 1909 366 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 366 275 186
BsiHKAI G_wGCw'C
Cuts at: 3577 4887 4972 6043 3577
Size: 1310 85 1071 4360
Fragments arranged by size:
4360 1310 1071 85
BsmI GAATG_Cn'
Cuts at: 2914 3005 3995 4337 4430 2914
Size: 91 990 342 93 5310
Fragments arranged by size:
5310 990 342 93 91
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2730 3577 4145 4887 4972 6043 1391
Size: 399 940 847 568 742 85 1071 2174
Fragments arranged by size:
2174 1071 940 847 742 568 399 85
BspEI T'CCGG_A
Cuts at: 6489 6489
Size: 6826
BspHI T'CATG_A
Cuts at: 2401 4715 6445 2401
Size: 2314 1730 2782
Fragments arranged by size:
2782 2314 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 3781 4181 844
Size: 1757 1180 400 3489
Fragments arranged by size:
3489 1757 1180 400
BsrBI CCG'CTC
Cuts at: 1394 1823 4713 1394
Size: 429 2890 3507
Fragments arranged by size:
3507 2890 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2166 2716 5321 5495 65
Size: 1183 918 550 2605 174 1396
Fragments arranged by size:
2605 1396 1183 918 550 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5470 5657 1700
Size: 110 3660 187 2869
Fragments arranged by size:
3660 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2300 96
Size: 2204 4622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2909 794
Size: 2115 4711
BstBI TT'CG_AA
Cuts at: 2381 5630 2381
Size: 3249 3577
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2212 3439 4156 4523 4908 4925 6377 6463
Size: 240 1227 717 367 385 17 1452 86
Cuts at: 6463 6475 6821 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1227 717 385 367 346 240
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5335
Fragments arranged by size:
5335 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2037 487
Size: 683 56 811 5276
Fragments arranged by size:
5276 811 683 56
BtsI GCAGTG_nn'
Cuts at: 4342 5136 5156 4342
Size: 794 20 6012
Fragments arranged by size:
6012 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2424 2459 529
Size: 35 1860 35 4896
Fragments arranged by size:
4896 1860 35 35
DraI TTT'AAA
Cuts at: 3535 4130 3535
Size: 595 6231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3033 5833 809
Size: 507 1717 2800 1802
Fragments arranged by size:
2800 1802 1717 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3907 4273 5162
Size: 54 1425 50 275 18 2077 366 889
Cuts at: 5162 6791 8
Size: 1629 43
Fragments arranged by size:
2077 1629 1425 889 366 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 4273 1537
Size: 275 2095 366 4090
Fragments arranged by size:
4090 2095 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3801 4756 1353
Size: 2448 955 3423
Fragments arranged by size:
3423 2448 955
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5413 5787 5933 387
Size: 1064 138 3824 374 146 1280
Fragments arranged by size:
3824 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2505 3498 4889 5486 6273 6541 1239
Size: 393 873 993 1391 597 787 268 1524
Fragments arranged by size:
1524 1391 993 873 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3414 4135 4513 3414
Size: 721 378 5727
Fragments arranged by size:
5727 721 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4099 4099
Size: 6826
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 5973 1678
Size: 4295 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2497 2529 3431 3463 3752
Size: 32 135 32 925 32 902 32 289
Cuts at: 3752 3784 5467 5499 5541 5573 1373
Size: 32 1683 32 42 32 2626
Fragments arranged by size:
2626 1683 925 902 289 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4170 4416 669
Size: 988 281 2232 246 3079
Fragments arranged by size:
3079 2232 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6826
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 3029 3732 4425 3029
Size: 703 693 5430
Fragments arranged by size:
5430 703 693
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3076 5940 6124 1305
Size: 1771 2864 184 2007
Fragments arranged by size:
2864 2007 1771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5004
Fragments arranged by size:
5004 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3603 4784 5143 5302 518
Size: 648 2437 1181 359 159 2042
Fragments arranged by size:
2437 2042 1181 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2467 4242 4919 6067 6312 1606
Size: 861 1775 677 1148 245 2120
Fragments arranged by size:
2120 1775 1148 861 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5335
Fragments arranged by size:
5335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NsiI A_TGCA'T
Cuts at: 2260 2260
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 2501 2833 2889 3276 3780 4184 5729
Size: 97 1272 332 56 387 504 404 1545
Cuts at: 5729 1132
Size: 2229
Fragments arranged by size:
2229 1545 1272 504 404 387 332 97
56
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6826
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6826
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PmlI CAC'GTG
Cuts at: 2533 2533
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4895 4927 6408 6440 4895
Size: 32 1481 32 5281
Fragments arranged by size:
5281 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 2541 4396 2541
Size: 1855 4971
PspXI vC'TCGA_Gb
Cuts at: 2799 2799
Size: 6826
PstI C_TGCA'G
Cuts at: 830 2103 2466 4178 830
Size: 1273 363 1712 3478
Fragments arranged by size:
3478 1712 1273 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2467 2467
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SfcI C'TryA_G
Cuts at: 826 1045 2099 2462 2969 3771 4174 5309 5990
Size: 219 1054 363 507 802 403 1135 681
Cuts at: 5990 6181 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1054 802 681 507 403 363
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 4153 1872
Size: 2281 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 2799 3063 3586 4930 5831
Size: 197 926 654 202 264 523 1344 901
Cuts at: 5831 6093 820
Size: 262 1553
Fragments arranged by size:
1553 1344 926 901 654 523 264 262
202 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 3406 3721 4122 4750 5
Size: 47 3354 315 401 628 2081
Fragments arranged by size:
3354 2081 628 401 315 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6826
StyI C'CwwG_G
Cuts at: 513 1065 2004 3372 3567 3677 4265 513
Size: 552 939 1368 195 110 588 3074
Fragments arranged by size:
3074 1368 939 588 552 195 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4853 4870 5023 5208 5653 325
Size: 1255 3273 17 153 185 445 1498
Fragments arranged by size:
3273 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2086 2300 2778
Size: 274 80 33 51 1443 109 214 478
Cuts at: 2778 5072 6784 96
Size: 2294 1712 138
Fragments arranged by size:
2294 1712 1443 478 274 214 138 109
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6786 6818 6786
Size: 32 6794
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6826
XbaI T'CTAG_A
Cuts at: 3118 4162 3118
Size: 1044 5782
XhoI C'TCGA_G
Cuts at: 1943 2799 1943
Size: 856 5970
XmaI C'CCGG_G
Cuts at: 1870 4151 1870
Size: 2281 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 4955 2583
Size: 2372 4454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI