Restriction map for
pFN21AE5786
(Linear) (Six-base) MAPSORT of: KIEE5786.seq Check: 2402 from: 1 to: 2121
KIEE5786.orf
With 182 enzymes: SgfI *
December 5, 2014 13:11 ..
AccI GT'mk_AC
Cuts at: 0 350 558 2121
Size: 350 208 1563
Fragments arranged by size:
1563 350 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 2121
Size: 500 1621
AlwNI CAG_nnn'CTG
Cuts at: 0 102 2121
Size: 102 2019
ApoI r'AATT_y
Cuts at: 0 323 378 1409 1483 1739 1974 2121
Size: 323 55 1031 74 256 235 147
Fragments arranged by size:
1031 323 256 235 147 74 55
AvaI C'yCGr_G
Cuts at: 0 794 1538 2121
Size: 794 744 583
Fragments arranged by size:
794 744 583
BamHI G'GATC_C
Cuts at: 0 207 2121
Size: 207 1914
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 877 2121
Size: 296 581 1244
Fragments arranged by size:
1244 581 296
BfrBI ATG'CAT
Cuts at: 0 253 2121
Size: 253 1868
Bme1580I G_kGCm'C
Cuts at: 0 725 2121
Size: 725 1396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1493 2121
Size: 1493 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1043 1566 2121
Size: 613 430 523 555
Fragments arranged by size:
613 555 523 430
BsaAI yAC'GTr
Cuts at: 0 528 2121
Size: 528 1593
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1668 1698 2121
Size: 1668 30 423
Fragments arranged by size:
1668 423 30
BseYI C'CCAG_C
Cuts at: 0 2053 2121
Size: 2053 68
BsiEI CG_ry'CG
Cuts at: 0 1905 2121
Size: 1905 216
BsiHKAI G_wGCw'C
Cuts at: 0 1572 2121
Size: 1572 549
BsmI GAATG_Cn'
Cuts at: 0 909 1000 1990 2121
Size: 909 91 990 131
Fragments arranged by size:
990 909 131 91
Bsp1286I G_dGCh'C
Cuts at: 0 725 1572 2121
Size: 725 847 549
Fragments arranged by size:
847 725 549
BspHI T'CATG_A
Cuts at: 0 396 2121
Size: 396 1725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 1776 2121
Size: 596 1180 345
Fragments arranged by size:
1180 596 345
BsrDI GCAATG_nn'
Cuts at: 0 161 711 2121
Size: 161 550 1410
Fragments arranged by size:
1410 550 161
BsrGI T'GTAC_A
Cuts at: 0 295 2121
Size: 295 1826
BstAPI GCAn_nnn'nTGC
Cuts at: 0 904 2121
Size: 904 1217
BstBI TT'CG_AA
Cuts at: 0 376 2121
Size: 376 1745
BstYI r'GATC_y
Cuts at: 0 207 1434 2121
Size: 207 1227 687
Fragments arranged by size:
1227 687 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 32 2121
Size: 32 2089
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 419 454 2121
Size: 419 35 1667
Fragments arranged by size:
1667 419 35
DraI TTT'AAA
Cuts at: 0 1530 2121
Size: 1530 591
DrdI GACnn_nn'nnGTC
Cuts at: 0 1028 2121
Size: 1028 1093
EaeI y'GGCC_r
Cuts at: 0 1902 2121
Size: 1902 219
EagI C'GGCC_G
Cuts at: 0 1902 2121
Size: 1902 219
EarI CTCTTCn'nnn_
Cuts at: 0 1796 2121
Size: 1796 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 1493 2121
Size: 500 993 628
Fragments arranged by size:
993 628 500
EcoRI G'AATT_C
Cuts at: 0 1409 2121
Size: 1409 712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2094 2121
Size: 2094 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 524 1426 1458 1747 1779 2121
Size: 492 32 902 32 289 32 342
Fragments arranged by size:
902 492 342 289 32 32 32
MfeI C'AATT_G
Cuts at: 0 1024 1727 2121
Size: 1024 703 394
Fragments arranged by size:
1024 703 394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 2121
Size: 1071 1050
MslI CAynn'nnrTG
Cuts at: 0 1598 2121
Size: 1598 523
MspA1I CmG'CkG
Cuts at: 0 462 2121
Size: 462 1659
NsiI A_TGCA'T
Cuts at: 0 255 2121
Size: 255 1866
NspI r_CATG'y
Cuts at: 0 496 828 884 1271 1775 2121
Size: 496 332 56 387 504 346
Fragments arranged by size:
504 496 387 346 332 56
PmlI CAC'GTG
Cuts at: 0 528 2121
Size: 528 1593
PsiI TTA'TAA
Cuts at: 0 536 2121
Size: 536 1585
PspXI vC'TCGA_Gb
Cuts at: 0 794 2121
Size: 794 1327
PstI C_TGCA'G
Cuts at: 0 98 461 2121
Size: 98 363 1660
Fragments arranged by size:
1660 363 98
PvuII CAG'CTG
Cuts at: 0 462 2121
Size: 462 1659
SfcI C'TryA_G
Cuts at: 0 94 457 964 1766 2121
Size: 94 363 507 802 355
Fragments arranged by size:
802 507 363 355 94
SmlI C'TyrA_G
Cuts at: 0 592 794 1058 1581 2121
Size: 592 202 264 523 540
Fragments arranged by size:
592 540 523 264 202
SspI AAT'ATT
Cuts at: 0 1401 1716 2117 2121
Size: 1401 315 401 4
Fragments arranged by size:
1401 401 315 4
StyI C'CwwG_G
Cuts at: 0 1367 1562 1672 2121
Size: 1367 195 110 449
Fragments arranged by size:
1367 449 195 110
TatI w'GTAC_w
Cuts at: 0 81 295 773 2121
Size: 81 214 478 1348
Fragments arranged by size:
1348 478 214 81
XbaI T'CTAG_A
Cuts at: 0 1113 2121
Size: 1113 1008
XhoI C'TCGA_G
Cuts at: 0 794 2121
Size: 794 1327
XmnI GAAnn'nnTTC
Cuts at: 0 578 2121
Size: 578 1543
Enzymes that do cut:
AccI AcuI AlwNI ApoI AvaI BamHI BbsI BfrBI
Bme1580I BpmI BpuEI BsaAI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstBI
BstYI BtgZI CspCI DraI DrdI EaeI EagI EarI
Eco57MI EcoRI FalI Hin4I MfeI MmeI MslI MspA1I
NsiI NspI PmlI PsiI PspXI PstI PvuII SfcI
SmlI SspI StyI TatI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsgI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgI BtsI ClaI DraIII EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XcmI XmaI ZraI