(Circular) (Six-base) MAPSORT of: pFN21AE9000.seq Check: 7067 from: 1 to: 6268 pFN21AE9000 6268 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3611 1937 Size: 1674 4594 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4331 5715 5983 1632 Size: 2699 1384 268 1917 Fragments arranged by size: 2699 1917 1384 268 AfeI AGC'GCT Cuts at: 2633 2633 Size: 6268 AflII C'TTAA_G Cuts at: 820 1017 2743 820 Size: 197 1726 4345 Fragments arranged by size: 4345 1726 197 AflIII A'CryG_T Cuts at: 1203 2761 2957 3067 5167 6144 1203 Size: 1558 196 110 2100 977 1327 Fragments arranged by size: 2100 1558 1327 977 196 110 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 2336 1166 Size: 1170 5098 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 4011 5583 4011 Size: 1572 4696 ApaLI G'TGCA_C Cuts at: 2166 2230 2800 4325 5481 2166 Size: 64 570 1525 1156 2953 Fragments arranged by size: 2953 1525 1156 570 64 ApoI r'AATT_y Cuts at: 1493 3577 3803 3955 6064 1493 Size: 2084 226 152 2109 1697 Fragments arranged by size: 2109 2084 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 2434 3593 1870 Size: 73 491 1159 4545 Fragments arranged by size: 4545 1159 491 73 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 2438 3589 5044 618 Size: 325 217 627 487 164 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 627 487 325 217 164 BanII G_rGCy'C Cuts at: 1391 2102 2115 3587 1391 Size: 711 13 1472 4072 Fragments arranged by size: 4072 1472 711 13 BbeI G_GCGC'C Cuts at: 2278 2278 Size: 6268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4459 4493 1903 Size: 34 2522 34 3678 Fragments arranged by size: 3678 2522 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 2119 1548 Size: 571 5697 BfrBI ATG'CAT Cuts at: 2985 2985 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2856 4879 Size: 107 122 71 631 605 337 847 2023 Cuts at: 4879 136 Size: 1525 Fragments arranged by size: 2023 1525 847 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6263 6263 Size: 6268 BlpI GC'TnA_GC Cuts at: 3685 3685 Size: 6268 Bme1580I G_kGCm'C Cuts at: 1790 2170 2234 2441 2804 4329 5485 1790 Size: 380 64 207 363 1525 1156 2573 Fragments arranged by size: 2573 1525 1156 380 363 207 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 2479 2496 Size: 416 53 83 399 24 1025 37 17 Cuts at: 2496 3242 3386 3416 3999 4957 442 Size: 746 144 30 583 958 1753 Fragments arranged by size: 1753 1025 958 746 583 416 399 144 83 53 37 30 24 17 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4928 1239 Size: 3689 2579 Bpu10I CC'TnA_GC Cuts at: 2096 2096 Size: 6268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2713 4393 5258 5556 2713 Size: 1680 865 298 3425 Fragments arranged by size: 3425 1680 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2395 2676 2990 3505 4931 882 Size: 617 896 281 314 515 1426 2219 Fragments arranged by size: 2219 1426 896 617 515 314 281 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 6262 6262 Size: 6268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2275 4075 4457 5092 Size: 53 83 186 936 742 1800 382 635 Cuts at: 5092 275 Size: 1451 Fragments arranged by size: 1800 1451 936 742 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2649 4701 5099 5373 5520 5931 2649 Size: 2052 398 274 147 411 2986 Fragments arranged by size: 2986 2052 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2600 1178 Size: 30 165 30 1167 30 4846 Fragments arranged by size: 4846 1167 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6268 BseYI C'CCAG_C Cuts at: 2363 5471 5967 2363 Size: 3108 496 2664 Fragments arranged by size: 3108 2664 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2916 3321 1163 Size: 573 1180 405 4110 Fragments arranged by size: 4110 1180 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2102 2170 2234 2804 4329 4414 5485 2102 Size: 68 64 570 1525 85 1071 2885 Fragments arranged by size: 2885 1525 1071 570 85 68 64 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2115 2170 2234 2441 2804 3587 Size: 399 312 13 55 64 207 363 783 Cuts at: 3587 4329 4414 5485 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 399 363 312 207 85 64 55 13 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 2674 4157 5887 2674 Size: 1483 1730 3055 Fragments arranged by size: 3055 1730 1483 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3623 844 Size: 2779 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2129 2397 3273 3513 4763 4937 65 Size: 1183 881 268 876 240 1250 174 1396 Fragments arranged by size: 1396 1250 1183 881 876 268 240 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 2238 794 Size: 1444 4824 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2338 Size: 6268 BstYI r'GATC_y Cuts at: 1972 3598 3965 4350 4367 5819 5905 5917 6263 Size: 1626 367 385 17 1452 86 12 346 Cuts at: 6263 1972 Size: 1977 Fragments arranged by size: 1977 1626 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2160 2270 2160 Size: 110 6158 BtgI C'CryG_G Cuts at: 513 1065 2004 2106 2334 3168 513 Size: 552 939 102 228 834 3613 Fragments arranged by size: 3613 939 834 552 228 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2344 487 Size: 683 56 1118 4411 Fragments arranged by size: 4411 1118 683 56 BtsI GCAGTG_nn' Cuts at: 2461 2713 2802 3414 3784 4578 4598 2461 Size: 252 89 612 370 794 20 4131 Fragments arranged by size: 4131 794 612 370 252 89 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DraIII CAC_nnn'GTG Cuts at: 2845 2845 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5275 809 Size: 507 3959 1802 Fragments arranged by size: 3959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2315 3080 3715 Size: 54 1425 50 275 18 485 765 635 Cuts at: 3715 4604 6233 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 765 635 485 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2988 4198 1353 Size: 1635 1210 3423 Fragments arranged by size: 3423 1635 1210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4855 5229 5375 387 Size: 1064 138 3266 374 146 1280 Fragments arranged by size: 3266 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2100 2100 Size: 6268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4331 4928 5715 5983 1239 Size: 393 2699 597 787 268 1524 Fragments arranged by size: 2699 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 6268 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 2278 2635 5415 1678 Size: 600 357 2780 2531 Fragments arranged by size: 2780 2531 600 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 4909 4941 4983 Size: 32 135 32 996 32 2309 32 42 Cuts at: 4983 5015 1373 Size: 32 2626 Fragments arranged by size: 2626 2309 996 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2415 3307 3397 3612 3858 669 Size: 988 281 477 892 90 215 246 3079 Fragments arranged by size: 3079 988 892 477 281 246 215 90 HindIII A'AGCT_T Cuts at: 748 2046 2247 3543 748 Size: 1298 201 1296 3473 Fragments arranged by size: 3473 1298 1296 201 HpaI GTT'AAC Cuts at: 3307 3858 3307 Size: 551 5717 KasI G'GCGC_C Cuts at: 2274 2274 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 3867 3867 Size: 6268 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5382 5566 1305 Size: 4077 184 2007 Fragments arranged by size: 4077 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3082 10 Size: 54 1425 343 1250 3196 Fragments arranged by size: 3196 1425 1250 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2227 2336 2471 2529 2595 3476 4226 Size: 648 1061 109 135 58 66 881 750 Cuts at: 4226 4585 4744 518 Size: 359 159 2042 Fragments arranged by size: 2042 1061 881 750 648 359 159 135 109 66 58 MspA1I CmG'CkG Cuts at: 1606 3263 3684 4361 5509 5754 1606 Size: 1657 421 677 1148 245 2120 Fragments arranged by size: 2120 1657 1148 677 421 245 NarI GG'CG_CC Cuts at: 2275 2275 Size: 6268 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4777 Fragments arranged by size: 4777 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NsiI A_TGCA'T Cuts at: 2987 2987 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 2765 2961 2985 3071 3529 3626 5171 Size: 97 1536 196 24 86 458 97 1545 Cuts at: 5171 1132 Size: 2229 Fragments arranged by size: 2229 1545 1536 458 196 97 97 86 24 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 2761 2957 3067 5167 2761 Size: 196 110 2100 3862 Fragments arranged by size: 3862 2100 196 110 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PstI C_TGCA'G Cuts at: 830 2243 3620 830 Size: 1413 1377 3478 Fragments arranged by size: 3478 1413 1377 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 PvuII CAG'CTG Cuts at: 3263 3263 Size: 6268 SacI G_AGCT'C Cuts at: 2102 2102 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2239 3516 3616 4751 5432 5623 826 Size: 219 1194 1277 100 1135 681 191 1471 Fragments arranged by size: 1471 1277 1194 1135 681 219 191 100 SfoI GGC'GCC Cuts at: 2276 2276 Size: 6268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2728 2743 4372 5273 5535 820 Size: 197 926 785 15 1629 901 262 1553 Fragments arranged by size: 1629 1553 926 901 785 262 197 15 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3529 3626 1132 Size: 2397 97 3774 Fragments arranged by size: 3774 2397 97 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 2549 2734 1517 Size: 1032 185 5051 Fragments arranged by size: 5051 1032 185 StyI C'CwwG_G Cuts at: 513 1065 2004 3563 3707 513 Size: 552 939 1559 144 3074 Fragments arranged by size: 3074 1559 939 552 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4295 4312 4465 4650 5095 325 Size: 1255 2715 17 153 185 445 1498 Fragments arranged by size: 2715 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4514 6226 96 Size: 274 80 33 51 1443 2537 1712 138 Fragments arranged by size: 2537 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2326 2358 2570 2602 6228 6260 2326 Size: 32 212 32 3626 32 2334 Fragments arranged by size: 3626 2334 212 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2485 3238 1316 Size: 1169 753 4346 Fragments arranged by size: 4346 1169 753 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 2549 2723 4397 2549 Size: 174 1674 4420 Fragments arranged by size: 4420 1674 174 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI