################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AviII BanI BbeI BbvCI BfrI BglI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BstAPI Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I FseI FspI KasI KpnI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacI SalI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 145 GACGTC GACGT^C AcyI 145 GACGTC G[GA]^CG[CT]C AflIII 2586 ACATGT A^C[GA][CT]GT AseI 3289 ATTAAT AT^TAAT AsnI 3289 ATTAAT AT^TAAT Asp700I 3955 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4260 CCTAGG C^CTAGG BalI 4 TGGCCA TGG^CCA BbrPI 2640 CACGTG CAC^GTG BcgI 3415 CGAACACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 4260 CCTAGG C^CTAGG BsaHI 145 GACGTC G[GA]^CG[CT]C BsaWI 1391 ACCGGA [AT]^CCGG[AT] BseRI 3937 GAGGAG GAGGAG(10/8) Bsp1407I 1809 TGTACA T^GTACA BspLU11I 2586 ACATGT A^CATGT BspMI 301 GCAGGT ACCTGC(4/8) BsrGI 1809 TGTACA T^GTACA BssSI 3934 CACGAG CACGAG(-5/-1) BstBI 3545 TTCGAA TT^CGAA BstEII 3667 GGTAACC G^GT[ACGT]ACC BstPI 3667 GGTAACC G^GT[ACGT]ACC DraIII 1147 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 3163 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3667 GGTAACC G^GT[ACGT]ACC EcoRI 953 GAATTC G^AATTC EcoRV 1276 GATATC GAT^ATC EcoT22I 1955 ATGCAT ATGCA^T FauI 2708 GCGGG CCCGC(4/6) Hin1I 145 GACGTC G[GA]^CG[CT]C HpaI 1924 GTTAAC GTT^AAC KspI 2706 CCGCGG CCGC^GG MfeI 865 CAATTG C^AATTG MluNI 4 TGGCCA TGG^CCA MscI 4 TGGCCA TGG^CCA MunI 865 CAATTG C^AATTG NdeI 911 CATATG CA^TATG NsiI 1955 ATGCAT ATGCA^T NspV 3545 TTCGAA TT^CGAA PflFI 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2640 CACGTG CAC^GTG PmlI 2640 CACGTG CAC^GTG PshAI 322 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3289 ATTAAT AT^TAAT SacII 2706 CCGCGG CCGC^GG SbfI 1564 CCTGCAGG CCTGCA^GG SfuI 3545 TTCGAA TT^CGAA Sse8387I 1564 CCTGCAGG CCTGCA^GG SspBI 1809 TGTACA T^GTACA StuI 1797 AGGCCT AGG^CCT Tth111I 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3955 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccII 2707 CGCG CG^CG 4312 CGCG AlwNI 302 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1641 CAGCAACTG Aor51HI 1883 AGCGCT AGC^GCT 3827 AGCGCT AspHI 2112 GAGCAC G[AT]GC[AT]^C 4093 GAGCAC AvaI 1508 CCCGAG C^[CT]CG[GA]G 4363 CCCGAG BamHI 1620 GGATCC G^GATCC 4046 GGATCC BanII 1347 GAGCCC G[GA]GC[CT]^C 2710 GGGCTC BclI 1980 TGATCA T^GATCA 2636 TGATCA BcnI 1776 CCGGG CC^[GC]GG 1853 CCCGG BglII 1635 AGATCT A^GATCT 4104 AGATCT BsaAI 2640 CACGTG [CT]AC^GT[GA] 2933 CACGTA BsaBI 2679 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2784 GATTGGGATC BsaI 1964 GAGACC GGTCTC(1/5) 3754 GAGACC BsiHKAI 2112 GAGCAC G[AT]GC[AT]^C 4093 GAGCAC BsoBI 1508 CCCGAG C^[CT]CG[GA]G 4363 CCCGAG BsrFI 4209 ACCGGC [GA]^CCGG[CT] 4383 ACCGGC Bst1107I 424 GTATAC GTA^TAC 4000 GTATAC BstUI 2707 CGCG CG^CG 4312 CGCG BstXI 456 CCACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3902 CCATTGTCCTGG BstZ17I 424 GTATAC GTA^TAC 4000 GTATAC Cfr10I 4209 ACCGGC [GA]^CCGG[CT] 4383 ACCGGC Eco47III 1883 AGCGCT AGC^GCT 3827 AGCGCT FbaI 1980 TGATCA T^GATCA 2636 TGATCA HaeII 1883 AGCGCT [GA]GCGC^[CT] 3827 AGCGCT HgaI 3491 GACGC GACGC(5/10) 3595 GCGTC HincII 1924 GTTAAC GT[CT]^[GA]AC 2496 GTCAAC HindII 1924 GTTAAC GT[CT]^[GA]AC 2496 GTCAAC MamI 2679 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2784 GATTGGGATC MvnI 2707 CGCG CG^CG 4312 CGCG NciI 1776 CCGGG CC^[GC]GG 1853 CCCGG PflMI 1905 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3658 CCACTGAATGG SapI 2775 GCTCTTC GCTCTTC(1/4) 3241 GCTCTTC ScaI 1396 AGTACT AGT^ACT 2574 AGTACT SpeI 1399 ACTAGT A^CTAGT 4399 ACTAGT SphI 4142 GCATGC GCATG^C 4250 GCATGC SspI 1157 AATATT AAT^ATT 2086 AATATT Van91I 1905 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3658 CCACTGAATGG XcmI 1781 CCATGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2279 CCAGTATGAAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII