Restriction map for
pFN21AE3772
##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
ApaI AscI AvaI AviII AvrII
BamHI BbrPI BbvCI BciVI BglI
BglII BlnI BsaAI BsiEI BsiWI
BsmBI BsoBI BspDI BspLU11I BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HindIII HpaI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 878 GGTACC G^GTACC
AcyI 4564 GGCGCC G[GA]^CG[CT]C
AflII 3435 CTTAAG C^TTAAG
AflIII 2211 ACACGT A^C[GA][CT]GT
AhdI 3145 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1282 CCTGAGG CC^T[ACGT]AGG
AseI 1915 ATTAAT AT^TAAT
AsnI 1915 ATTAAT AT^TAAT
Asp718I 878 GGTACC G^GTACC
AspEI 3145 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2437 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4564 GGCGCC GGCGC^C
BclI 1184 TGATCA T^GATCA
BcnI 2073 CCGGG CC^[GC]GG
BfrI 3435 CTTAAG C^TTAAG
BlpI 81 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC
BsaHI 4564 GGCGCC G[GA]^CG[CT]C
BseRI 4193 GAGGAG GAGGAG(10/8)
BsrBI 103 GAGCGG CCGCTC(-3/-3)
BssSI 2720 CTCGTG CACGAG(-5/-1)
BstEII 1921 GGTGACC G^GT[ACGT]ACC
BstPI 1921 GGTGACC G^GT[ACGT]ACC
Bsu36I 1282 CCTGAGG CC^T[ACGT]AGG
CelII 81 GCTCAGC GC^T[ACGT]AGC
DrdI 4755 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3145 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1282 CCTGAGG CC^T[ACGT]AGG
EcoNI 1282 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1921 GGTGACC G^GT[ACGT]ACC
FauI 105 GCGGG CCCGC(4/6)
FbaI 1184 TGATCA T^GATCA
Hin1I 4564 GGCGCC G[GA]^CG[CT]C
HincII 181 GTTGAC GT[CT]^[GA]AC
HindII 181 GTTGAC GT[CT]^[GA]AC
KasI 4564 GGCGCC G^GCGCC
KpnI 878 GGTACC GGTAC^C
KspI 15 CCGCGG CCGC^GG
NarI 4564 GGCGCC GG^CGCC
NciI 2073 CCGGG CC^[GC]GG
PflFI 2437 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2258 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4526 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1915 ATTAAT AT^TAAT
SacI 88 GAGCTC GAGCT^C
SacII 15 CCGCGG CCGC^GG
SapI 3748 GCTCTTC GCTCTTC(1/4)
SbfI 2222 CCTGCAGG CCTGCA^GG
SfoI 4564 GGCGCC GGC^GCC
Sse8387I 2222 CCTGCAGG CCTGCA^GG
Tth111I 2437 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2258 CCATACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccIII 425 TCCGGA T^CCGGA
3686 TCCGGA
Alw44I 1294 GTGCAC G^TGCAC
4605 GTGCAC
ApaLI 1294 GTGCAC G^TGCAC
4605 GTGCAC
BcgI 291 CGAACCTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1886 GCATGGAAGTCG
BfaI 1927 CTAG C^TAG
4234 CTAG
BseAI 425 TCCGGA T^CCGGA
3686 TCCGGA
BspEI 425 TCCGGA T^CCGGA
3686 TCCGGA
HaeII 37 AGCGCC [GA]GCGC^[CT]
4564 GGCGCC
HgaI 4620 GACGC GACGC(5/10)
3683 GCGTC
MaeI 1927 CTAG C^TAG
4234 CTAG
MroI 425 TCCGGA T^CCGGA
3686 TCCGGA
NcoI 2981 CCATGG C^CATGG
3024 CCATGG
PpuMI 44 AGGACCT [GA]G^G[AT]CC[CT]
1513 AGGACCT
SphI 446 GCATGC GCATG^C
2097 GCATGC
StuI 1279 AGGCCT AGG^CCT
4420 AGGCCT
TaqI 803 TCGA T^CGA
1728 TCGA
TthHB8I 803 TCGA T^CGA
1728 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmnI