(Linear) (Six-base) MAPSORT of: KIEE3772.seq Check: 5536 from: 1 to: 4818 KIEE3772.orf With 182 enzymes: SgfI * July 3, 2012 18:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 733 3931 4818 Size: 733 3198 887 Fragments arranged by size: 3198 887 733 Acc65I G'GTAC_C Cuts at: 0 878 4818 Size: 878 3940 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 1058 2520 2626 2749 2978 3082 4818 Size: 571 487 1462 106 123 229 104 1736 Fragments arranged by size: 1736 1462 571 487 229 123 106 104 AflII C'TTAA_G Cuts at: 0 3435 4818 Size: 3435 1383 AflIII A'CryG_T Cuts at: 0 2211 4818 Size: 2211 2607 AhdI GACnn_n'nnGTC Cuts at: 0 3150 4818 Size: 3150 1668 AleI CACnn'nnGTG Cuts at: 0 907 2789 4818 Size: 907 1882 2029 Fragments arranged by size: 2029 1882 907 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 751 4612 4818 Size: 751 3861 206 Fragments arranged by size: 3861 751 206 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1392 1424 4700 4732 4818 Size: 1392 32 3276 32 86 Fragments arranged by size: 3276 1392 86 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 2131 3084 3329 3371 4818 Size: 2131 953 245 42 1447 Fragments arranged by size: 2131 1447 953 245 42 ApaLI G'TGCA_C Cuts at: 0 1294 4605 4818 Size: 1294 3311 213 Fragments arranged by size: 3311 1294 213 ApoI r'AATT_y Cuts at: 0 256 761 1240 1657 2036 2380 2610 2650 Size: 256 505 479 417 379 344 230 40 Cuts at: 2650 3345 3595 3712 4205 4282 4368 4734 4818 Size: 695 250 117 493 77 86 366 84 Fragments arranged by size: 695 505 493 479 417 379 366 344 256 250 230 117 86 84 77 40 AseI AT'TA_AT Cuts at: 0 1916 4818 Size: 1916 2902 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 862 895 1109 1142 4001 4034 4818 Size: 862 33 214 33 2859 33 784 Fragments arranged by size: 2859 862 784 214 33 33 33 BanI G'GyrC_C Cuts at: 0 878 937 4564 4818 Size: 878 59 3627 254 Fragments arranged by size: 3627 878 254 59 BanII G_rGCy'C Cuts at: 0 92 2525 2950 4818 Size: 92 2433 425 1868 Fragments arranged by size: 2433 1868 425 92 BbeI G_GCGC'C Cuts at: 0 4568 4818 Size: 4568 250 BbsI GAAGACnn'nnnn_ Cuts at: 0 103 239 1456 1829 2128 2303 3059 3146 Size: 103 136 1217 373 299 175 756 87 Cuts at: 3146 4818 Size: 1672 Fragments arranged by size: 1672 1217 756 373 299 175 136 103 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 280 314 1875 1909 4818 Size: 280 34 1561 34 2909 Fragments arranged by size: 2909 1561 280 34 34 BclI T'GATC_A Cuts at: 0 1184 4818 Size: 1184 3634 BlpI GC'TnA_GC Cuts at: 0 82 4818 Size: 82 4736 Bme1580I G_kGCm'C Cuts at: 0 121 1298 2661 4609 4818 Size: 121 1177 1363 1948 209 Fragments arranged by size: 1948 1363 1177 209 121 BmgBI CAC'GTC Cuts at: 0 1431 4818 Size: 1431 3387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 76 145 1466 2348 3560 4645 4818 Size: 70 6 69 1321 882 1212 1085 173 Fragments arranged by size: 1321 1212 1085 882 173 70 69 6 Bpu10I CC'TnA_GC Cuts at: 0 4342 4612 4818 Size: 4342 270 206 Fragments arranged by size: 4342 270 206 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2276 3505 4818 Size: 2276 1229 1313 Fragments arranged by size: 2276 1313 1229 BsaI GGTCTCn'nnnn_ Cuts at: 0 1932 3034 4742 4818 Size: 1932 1102 1708 76 Fragments arranged by size: 1932 1708 1102 76 BsaBI GATnn'nnATC Cuts at: 0 872 1349 4154 4818 Size: 872 477 2805 664 Fragments arranged by size: 2805 872 664 477 BsaHI Gr'CG_yC Cuts at: 0 4565 4818 Size: 4565 253 BsaWI w'CCGG_w Cuts at: 0 425 3686 4751 4818 Size: 425 3261 1065 67 Fragments arranged by size: 3261 1065 425 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3153 3183 4700 4730 4818 Size: 3153 30 1517 30 88 Fragments arranged by size: 3153 1517 88 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 4208 4818 Size: 4208 610 BseYI C'CCAG_C Cuts at: 0 698 3087 3676 4560 4803 4818 Size: 698 2389 589 884 243 15 Fragments arranged by size: 2389 884 698 589 243 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 711 1188 2767 3089 3405 4605 4751 Size: 188 523 477 1579 322 316 1200 146 Cuts at: 4751 4818 Size: 67 Fragments arranged by size: 1579 1200 523 477 322 316 188 146 67 BsiHKAI G_wGCw'C Cuts at: 0 83 92 1298 2014 2292 4609 4818 Size: 83 9 1206 716 278 2317 209 Fragments arranged by size: 2317 1206 716 278 209 83 9 BsmI GAATG_Cn' Cuts at: 0 142 283 3942 4348 4818 Size: 142 141 3659 406 470 Fragments arranged by size: 3659 470 406 142 141 Bsp1286I G_dGCh'C Cuts at: 0 83 92 121 1298 2014 2292 2525 2661 Size: 83 9 29 1177 716 278 233 136 Cuts at: 2661 2950 4609 4818 Size: 289 1659 209 Fragments arranged by size: 1659 1177 716 289 278 233 209 136 83 29 9 BspEI T'CCGG_A Cuts at: 0 425 3686 4818 Size: 425 3261 1132 Fragments arranged by size: 3261 1132 425 BspHI T'CATG_A Cuts at: 0 1127 2907 3294 3453 4818 Size: 1127 1780 387 159 1365 Fragments arranged by size: 1780 1365 1127 387 159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 328 733 3931 4818 Size: 328 405 3198 887 Fragments arranged by size: 3198 887 405 328 BsrBI CCG'CTC Cuts at: 0 105 4818 Size: 105 4713 BsrDI GCAATG_nn' Cuts at: 0 1601 2204 2505 4818 Size: 1601 603 301 2313 Fragments arranged by size: 2313 1601 603 301 BsrGI T'GTAC_A Cuts at: 0 2589 2957 4778 4818 Size: 2589 368 1821 40 Fragments arranged by size: 2589 1821 368 40 BssSI C'ACGA_G Cuts at: 0 2720 4818 Size: 2720 2098 BstAPI GCAn_nnn'nTGC Cuts at: 0 2344 2495 3383 4818 Size: 2344 151 888 1435 Fragments arranged by size: 2344 1435 888 151 BstEII G'GTnAC_C Cuts at: 0 1921 4818 Size: 1921 2897 BstXI CCAn_nnnn'nTGG Cuts at: 0 48 958 4641 4818 Size: 48 910 3683 177 Fragments arranged by size: 3683 910 177 48 BstYI r'GATC_y Cuts at: 0 1864 2069 3660 4471 4818 Size: 1864 205 1591 811 347 Fragments arranged by size: 1864 1591 811 347 205 Bsu36I CC'TnA_GG Cuts at: 0 1283 4818 Size: 1283 3535 BtgI C'CryG_G Cuts at: 0 15 562 2981 3024 4653 4818 Size: 15 547 2419 43 1629 165 Fragments arranged by size: 2419 1629 547 165 43 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3336 4818 Size: 3336 1482 BtsI GCAGTG_nn' Cuts at: 0 593 963 2673 2779 2796 4515 4818 Size: 593 370 1710 106 17 1719 303 Fragments arranged by size: 1719 1710 593 370 303 106 17 DraI TTT'AAA Cuts at: 0 336 1097 2754 4056 4203 4818 Size: 336 761 1657 1302 147 615 Fragments arranged by size: 1657 1302 761 615 336 147 DrdI GACnn_nn'nnGTC Cuts at: 0 4761 4818 Size: 4761 57 EaeI y'GGCC_r Cuts at: 0 891 1535 2239 2393 3021 3815 4643 4818 Size: 891 644 704 154 628 794 828 175 Fragments arranged by size: 891 828 794 704 644 628 175 154 EarI CTCTTCn'nnn_ Cuts at: 0 1034 1583 2103 2360 3716 3755 4061 4818 Size: 1034 549 520 257 1356 39 306 757 Fragments arranged by size: 1356 1034 757 549 520 306 257 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1101 4818 Size: 1101 3717 EcoICRI GAG'CTC Cuts at: 0 90 4818 Size: 90 4728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 76 145 571 1058 1466 2348 2520 Size: 70 6 69 426 487 408 882 172 Cuts at: 2520 2626 2749 2978 3082 3560 4645 4818 Size: 106 123 229 104 478 1085 173 Fragments arranged by size: 1085 882 487 478 426 408 229 173 172 123 106 104 70 69 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1286 4818 Size: 1286 3532 EcoO109I rG'GnC_Cy Cuts at: 0 45 1514 2738 3548 4818 Size: 45 1469 1224 810 1270 Fragments arranged by size: 1469 1270 1224 810 45 EcoRI G'AATT_C Cuts at: 0 256 1657 3345 4818 Size: 256 1401 1688 1473 Fragments arranged by size: 1688 1473 1401 256 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 256 2317 4062 4818 Size: 256 2061 1745 756 Fragments arranged by size: 2061 1745 756 256 HaeII r_GCGC'y Cuts at: 0 41 4568 4818 Size: 41 4527 250 Fragments arranged by size: 4527 250 41 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 3136 3168 3681 3713 4818 Size: 3136 32 513 32 1105 Fragments arranged by size: 3136 1105 513 32 32 HincII GTy'rAC Cuts at: 0 183 4818 Size: 183 4635 KasI G'GCGC_C Cuts at: 0 4564 4818 Size: 4564 254 KpnI G_GTAC'C Cuts at: 0 882 4818 Size: 882 3936 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 650 734 1238 1590 2864 4503 4818 Size: 650 84 504 352 1274 1639 315 Fragments arranged by size: 1639 1274 650 504 352 315 84 MscI TGG'CCA Cuts at: 0 1537 2395 3023 3817 4645 4818 Size: 1537 858 628 794 828 173 Fragments arranged by size: 1537 858 828 794 628 173 MslI CAynn'nnrTG Cuts at: 0 733 907 956 2198 2789 2819 2980 3404 Size: 733 174 49 1242 591 30 161 424 Cuts at: 3404 4579 4818 Size: 1175 239 Fragments arranged by size: 1242 1175 733 591 424 239 174 161 49 30 MspA1I CmG'CkG Cuts at: 0 17 943 2050 2131 2485 4486 4592 4807 Size: 17 926 1107 81 354 2001 106 215 Cuts at: 4807 4818 Size: 11 Fragments arranged by size: 2001 1107 926 354 215 106 81 17 11 NarI GG'CG_CC Cuts at: 0 4565 4818 Size: 4565 253 NcoI C'CATG_G Cuts at: 0 2981 3024 4818 Size: 2981 43 1794 Fragments arranged by size: 2981 1794 43 NspI r_CATG'y Cuts at: 0 450 2101 2197 2422 4818 Size: 450 1651 96 225 2396 Fragments arranged by size: 2396 1651 450 225 96 PflMI CCAn_nnn'nTGG Cuts at: 0 2264 4818 Size: 2264 2554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4700 4732 4818 Size: 4700 32 86 Fragments arranged by size: 4700 86 32 PpuMI rG'GwC_Cy Cuts at: 0 45 1514 4818 Size: 45 1469 3304 Fragments arranged by size: 3304 1469 45 PshAI GACnn'nnGTC Cuts at: 0 4530 4818 Size: 4530 288 PsiI TTA'TAA Cuts at: 0 1157 2273 2708 4818 Size: 1157 1116 435 2110 Fragments arranged by size: 2110 1157 1116 435 PstI C_TGCA'G Cuts at: 0 2127 2227 3376 4818 Size: 2127 100 1149 1442 Fragments arranged by size: 2127 1442 1149 100 PvuII CAG'CTG Cuts at: 0 2050 2131 2485 4486 4807 4818 Size: 2050 81 354 2001 321 11 Fragments arranged by size: 2050 2001 354 321 81 11 SacI G_AGCT'C Cuts at: 0 92 4818 Size: 92 4726 SacII CC_GC'GG Cuts at: 0 18 4818 Size: 18 4800 SapI GCTCTTCn'nnn_ Cuts at: 0 3755 4818 Size: 3755 1063 SbfI CC_TGCA'GG Cuts at: 0 2227 4818 Size: 2227 2591 SfcI C'TryA_G Cuts at: 0 863 2102 2123 2223 3372 4685 4818 Size: 863 1239 21 100 1149 1313 133 Fragments arranged by size: 1313 1239 1149 863 133 100 21 SfoI GGC'GCC Cuts at: 0 4566 4818 Size: 4566 252 SmlI C'TyrA_G Cuts at: 0 2291 3435 3484 4818 Size: 2291 1144 49 1334 Fragments arranged by size: 2291 1334 1144 49 SphI G_CATG'C Cuts at: 0 450 2101 4818 Size: 450 1651 2717 Fragments arranged by size: 2717 1651 450 SspI AAT'ATT Cuts at: 0 364 777 3760 4818 Size: 364 413 2983 1058 Fragments arranged by size: 2983 1058 413 364 StuI AGG'CCT Cuts at: 0 1281 4422 4818 Size: 1281 3141 396 Fragments arranged by size: 3141 1281 396 StyI C'CwwG_G Cuts at: 0 41 482 2981 3024 3664 4771 4818 Size: 41 441 2499 43 640 1107 47 Fragments arranged by size: 2499 1107 640 441 47 43 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 115 1208 4818 Size: 115 1093 3610 Fragments arranged by size: 3610 1093 115 TatI w'GTAC_w Cuts at: 0 246 818 2231 2589 2724 2957 4328 4778 Size: 246 572 1413 358 135 233 1371 450 Cuts at: 4778 4818 Size: 40 Fragments arranged by size: 1413 1371 572 450 358 246 233 135 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 462 494 558 590 4818 Size: 462 32 64 32 4228 Fragments arranged by size: 4228 462 64 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2440 4818 Size: 2440 2378 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 648 2452 2979 4818 Size: 648 1804 527 1839 Fragments arranged by size: 1839 1804 648 527 XmnI GAAnn'nnTTC Cuts at: 0 2300 2627 4169 4283 4818 Size: 2300 327 1542 114 535 Fragments arranged by size: 2300 1542 535 327 114 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI BaeI BanI BanII BbeI BbsI BcgI BclI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PpiI PpuMI PshAI PsiI PstI PvuII SacI SacII SapI SbfI SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AgeI ApaI AscI AsiSI AvaI AvrII BamHI BbvCI BciVI BfrBI BglI BglII BmrI BmtI BplI BsaAI BsiEI BsiWI BsmBI BsrFI BssHII BstBI BstZ17I ClaI CspCI DraIII EagI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XhoI XmaI ZraI