################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII BbeI BbrPI BcgI BglI BlpI Bpu1102I BsiEI BsrBI BssHII Bst1107I BstEII BstPI BstZ17I CelII CfoI CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HhaI HinP1I KasI KspI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 4983 GGTACC G^GTACC AccII 6663 CGCG CG^CG AccIII 4439 TCCGGA T^CCGGA AclI 2800 AACGTT AA^CGTT AcyI 5357 GGCGTC G[GA]^CG[CT]C Alw44I 1069 GTGCAC G^TGCAC AocI 1033 CCTCAGG CC^T[ACGT]AGG ApaLI 1069 GTGCAC G^TGCAC AseI 7423 ATTAAT AT^TAAT AsnI 7423 ATTAAT AT^TAAT Asp718I 4983 GGTACC G^GTACC AvrII 6094 CCTAGG C^CTAGG BalI 6775 TGGCCA TGG^CCA BbvCI 3457 GCTGAGG CCTCAGC(-5/-2) BciVI 2361 GGATAC GTATCC(6/5) BlnI 6094 CCTAGG C^CTAGG BsaAI 2784 TACGTG [CT]AC^GT[GA] BsaHI 5357 GGCGTC G[GA]^CG[CT]C BseAI 4439 TCCGGA T^CCGGA BsgI 125 GTGCAG GTGCAG(16/14) BsiWI 2782 CGTACG C^GTACG BspDI 4135 ATCGAT AT^CGAT BspEI 4439 TCCGGA T^CCGGA BstUI 6663 CGCG CG^CG Bsu36I 1033 CCTCAGG CC^T[ACGT]AGG ClaI 4135 ATCGAT AT^CGAT DrdI 6720 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1033 CCTCAGG CC^T[ACGT]AGG EcoRV 4132 GATATC GAT^ATC FauI 7842 GCGGG CCCGC(4/6) Hin1I 5357 GGCGTC G[GA]^CG[CT]C HpaI 628 GTTAAC GTT^AAC KpnI 4983 GGTACC GGTAC^C MluNI 6775 TGGCCA TGG^CCA MroI 4439 TCCGGA T^CCGGA MscI 6775 TGGCCA TGG^CCA MvnI 6663 CGCG CG^CG PshAI 4187 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 7423 ATTAAT AT^TAAT Psp1406I 2800 AACGTT AA^CGTT SexAI 2013 ACCTGGT A^CC[AT]GGT SmaI 7503 CCCGGG CCC^GGG SpeI 102 ACTAGT A^CTAGT XmaCI 7503 CCCGGG C^CCGGG XmaI 7503 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 8279 CCGC CCGC(-3/-1) 7842 GCGG AflII 3135 CTTAAG C^TTAAG 7492 CTTAAG BamHI 2222 GGATCC G^GATCC 5721 GGATCC BfrI 3135 CTTAAG C^TTAAG 7492 CTTAAG BsaWI 4232 ACCGGA [AT]^CCGG[AT] 4439 TCCGGA Bsp1407I 81 TGTACA T^GTACA 8433 TGTACA BspMI 4502 ACCTGC ACCTGC(4/8) 5214 ACCTGC BsrDI 2405 CATTGC GCAATG(2/0) 3526 GCAATG BsrFI 6964 GCCGGT [GA]^CCGG[CT] 8041 ACCGGC BsrGI 81 TGTACA T^GTACA 8433 TGTACA BssSI 1637 CACGAG CACGAG(-5/-1) 4694 CACGAG BstBI 4067 TTCGAA TT^CGAA 4805 TTCGAA Cfr10I 6964 GCCGGT [GA]^CCGG[CT] 8041 ACCGGC DraI 5062 TTTAAA TTT^AAA 5230 TTTAAA DraIII 3058 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 5929 CACGGGGTG EaeI 4548 TGGCCG [CT]^GGCC[GA] 6775 TGGCCA NspV 4067 TTCGAA TT^CGAA 4805 TTCGAA SacI 3415 GAGCTC GAGCT^C 4598 GAGCTC SapI 1467 GAAGAGC GCTCTTC(1/4) 2197 GAAGAGC ScaI 2422 AGTACT AGT^ACT 6625 AGTACT SfuI 4067 TTCGAA TT^CGAA 4805 TTCGAA SspBI 81 TGTACA T^GTACA 8433 TGTACA XbaI 2092 TCTAGA T^CTAGA 6123 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI