(Circular) (Six-base) MAPSORT of: pFN21AE9679.seq Check: 4810 from: 1 to: 13351 pFN21AE9679 13351 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 11161 278 Size: 53 83 186 936 9625 2468 Fragments arranged by size: 9625 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2860 4080 4329 5913 10694 1937 Size: 923 1220 249 1584 4781 4594 Fragments arranged by size: 4781 4594 1584 1220 923 249 Acc65I G'GTAC_C Cuts at: 6988 10672 6988 Size: 3684 9667 AclI AA'CG_TT Cuts at: 1563 4806 11478 11851 1563 Size: 3243 6672 373 3063 Fragments arranged by size: 6672 3243 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2700 2849 3218 3660 3822 3987 4852 5198 Size: 1068 149 369 442 162 165 865 346 Cuts at: 5198 5846 6429 6705 8202 8505 8835 9959 11414 Size: 648 583 276 1497 303 330 1124 1455 Cuts at: 11414 12798 13066 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1497 1455 1384 1124 1068 865 648 583 442 369 346 330 303 276 268 165 162 149 AflII C'TTAA_G Cuts at: 820 1017 5140 9497 820 Size: 197 4123 4357 4674 Fragments arranged by size: 4674 4357 4123 197 AflIII A'CryG_T Cuts at: 1203 2278 3755 5819 7352 9637 12250 13227 1203 Size: 1075 1477 2064 1533 2285 2613 977 1327 Fragments arranged by size: 2613 2285 2064 1533 1477 1327 1075 977 AgeI A'CCGG_T Cuts at: 12182 12182 Size: 13351 AhdI GACnn_n'nnGTC Cuts at: 12080 12080 Size: 13351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 13351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 9066 10038 1184 Size: 7882 972 4497 Fragments arranged by size: 7882 4497 972 AlwNI CAG_nnn'CTG Cuts at: 2099 3095 6734 7160 8417 8735 9896 9899 10012 Size: 996 3639 426 1257 318 1161 3 113 Cuts at: 10012 11094 12666 2099 Size: 1082 1572 2784 Fragments arranged by size: 3639 2784 1572 1257 1161 1082 996 426 318 113 3 ApaLI G'TGCA_C Cuts at: 3074 11408 12564 3074 Size: 8334 1156 3861 Fragments arranged by size: 8334 3861 1156 ApoI r'AATT_y Cuts at: 1493 2122 2135 2237 2575 2596 2755 3001 3169 Size: 629 13 102 338 21 159 246 168 Cuts at: 3169 4003 4086 4148 4781 5174 5483 6122 7404 Size: 834 83 62 633 393 309 639 1282 Cuts at: 7404 7607 9396 9727 10660 10886 11038 13147 1493 Size: 203 1789 331 933 226 152 2109 1697 Fragments arranged by size: 2109 1789 1697 1282 933 834 639 633 629 393 338 331 309 246 226 203 168 159 152 102 83 62 21 13 AseI AT'TA_AT Cuts at: 160 1032 9429 11905 160 Size: 872 8397 2476 1606 Fragments arranged by size: 8397 2476 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13351 AvaI C'yCGr_G Cuts at: 1870 1943 3221 5778 6824 9508 10676 1870 Size: 73 1278 2557 1046 2684 1168 4545 Fragments arranged by size: 4545 2684 2557 1278 1168 1046 73 AvrII C'CTAG_G Cuts at: 8099 8099 Size: 13351 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5376 5409 5376 Size: 33 13318 BamHI G'GATC_C Cuts at: 4227 7726 10681 11048 4227 Size: 3499 2955 367 6530 Fragments arranged by size: 6530 3499 2955 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5081 5275 6988 7432 8966 Size: 325 217 627 3294 194 1713 444 1534 Cuts at: 8966 9206 10672 12127 618 Size: 240 1466 1455 1842 Fragments arranged by size: 3294 1842 1713 1534 1466 1455 627 444 325 240 217 194 BanII G_rGCy'C Cuts at: 1391 3656 5424 6607 8946 10670 1391 Size: 2265 1768 1183 2339 1724 4072 Fragments arranged by size: 4072 2339 2265 1768 1724 1183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3236 3782 3828 5111 7694 8169 8190 Size: 971 1337 546 46 1283 2583 475 21 Cuts at: 8190 9084 9571 10185 10524 10653 928 Size: 894 487 614 339 129 3626 Fragments arranged by size: 3626 2583 1337 1283 971 894 614 546 487 475 339 129 46 21 BbvCI CC'TCA_GC Cuts at: 5463 5463 Size: 13351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 11542 11576 1903 Size: 34 9605 34 3678 Fragments arranged by size: 9605 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4360 11243 12453 4360 Size: 6883 1210 5258 Fragments arranged by size: 6883 5258 1210 BclI T'GATC_A Cuts at: 1548 3130 8799 8989 1548 Size: 1582 5669 190 5910 Fragments arranged by size: 5910 5669 1582 190 BfrBI ATG'CAT Cuts at: 2081 2455 2660 2081 Size: 374 205 12772 Fragments arranged by size: 12772 374 205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 11962 136 Size: 107 122 71 631 605 10290 1525 Fragments arranged by size: 10290 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4453 6064 7273 13346 4453 Size: 1611 1209 6073 4458 Fragments arranged by size: 6073 4458 1611 1209 BlpI GC'TnA_GC Cuts at: 10768 10768 Size: 13351 Bme1580I G_kGCm'C Cuts at: 1790 3078 11412 12568 1790 Size: 1288 8334 1156 2573 Fragments arranged by size: 8334 2573 1288 1156 BmgBI CAC'GTC Cuts at: 1318 6194 1318 Size: 4876 8475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4780 5984 8940 Size: 416 53 83 399 24 3363 1204 2956 Cuts at: 8940 10414 11082 12040 442 Size: 1474 668 958 1753 Fragments arranged by size: 3363 2956 1753 1474 1204 958 668 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 13351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4146 5666 8292 8940 9648 10080 10371 12011 Size: 2907 1520 2626 648 708 432 291 1640 Cuts at: 12011 1239 Size: 2579 Fragments arranged by size: 2907 2626 2579 1640 1520 708 648 432 291 Bpu10I CC'TnA_GC Cuts at: 5423 5463 5423 Size: 40 13311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 9375 11476 12341 12639 9375 Size: 2101 865 298 10087 Fragments arranged by size: 10087 2101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 6442 8268 9134 12014 882 Size: 617 549 4394 1826 866 2880 2219 Fragments arranged by size: 4394 2880 2219 1826 866 617 549 BsaAI yAC'GTr Cuts at: 493 1204 4791 11142 493 Size: 711 3587 6351 2702 Fragments arranged by size: 6351 3587 2702 711 BsaBI GATnn'nnATC Cuts at: 2040 4452 5520 5844 6141 8389 13345 2040 Size: 2412 1068 324 297 2248 4956 2046 Fragments arranged by size: 4956 2412 2248 2046 1068 324 297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7363 11158 11540 12175 Size: 53 83 186 936 5830 3795 382 635 Cuts at: 12175 275 Size: 1451 Fragments arranged by size: 5830 3795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6237 6444 11784 12182 12456 12603 13014 6237 Size: 207 5340 398 274 147 411 6574 Fragments arranged by size: 6574 5340 411 398 274 207 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6439 6469 7263 7293 7681 Size: 30 165 30 5036 30 794 30 388 Cuts at: 7681 7711 8088 8118 8159 8189 9750 9780 1178 Size: 30 377 30 41 30 1561 30 4749 Fragments arranged by size: 5036 4749 1561 794 388 377 165 41 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4911 5480 6651 8073 8147 8180 8548 8551 Size: 3725 569 1171 1422 74 33 368 3 Cuts at: 8551 8554 8557 9331 10077 1186 Size: 3 3 774 746 4460 Fragments arranged by size: 4460 3725 1422 1171 774 746 569 368 74 33 3 3 3 BseYI C'CCAG_C Cuts at: 2269 7108 7225 8350 10025 12554 13050 2269 Size: 4839 117 1125 1675 2529 496 2570 Fragments arranged by size: 4839 2570 2529 1675 1125 496 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 1163 Size: 573 415 12363 Fragments arranged by size: 12363 573 415 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 10801 11562 12590 1540 Size: 275 186 8800 761 1028 2301 Fragments arranged by size: 8800 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3078 4276 4409 5424 5610 6607 8681 9088 9507 Size: 1198 133 1015 186 997 2074 407 419 Cuts at: 9507 11412 11497 12568 3078 Size: 1905 85 1071 3861 Fragments arranged by size: 3861 2074 1905 1198 1071 1015 997 419 407 186 133 85 BsiWI C'GTAC_G Cuts at: 4787 4787 Size: 13351 BsmI GAATG_Cn' Cuts at: 3025 3394 6491 6906 7670 9552 10044 10862 10955 Size: 369 3097 415 764 1882 492 818 93 Cuts at: 10955 3025 Size: 5421 Fragments arranged by size: 5421 3097 1882 818 764 492 415 369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5236 7370 9847 5236 Size: 2134 2477 8740 Fragments arranged by size: 8740 2477 2134 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3078 3656 4276 4409 5424 5610 6607 Size: 399 1288 578 620 133 1015 186 997 Cuts at: 6607 8681 8946 9088 9507 10670 11412 11497 12568 Size: 2074 265 142 419 1163 742 85 1071 Cuts at: 12568 1391 Size: 2174 Fragments arranged by size: 2174 2074 1288 1163 1071 1015 997 742 620 578 419 399 265 186 142 133 85 BspEI T'CCGG_A Cuts at: 6444 13014 6444 Size: 6570 6781 BspHI T'CATG_A Cuts at: 3304 5049 7900 9556 11240 12970 3304 Size: 1745 2851 1656 1684 1730 3685 Fragments arranged by size: 3685 2851 1745 1730 1684 1656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6516 7228 10706 844 Size: 5672 712 3478 3489 Fragments arranged by size: 5672 3489 3478 712 BsrBI CCG'CTC Cuts at: 1394 1823 11238 1394 Size: 429 9415 3507 Fragments arranged by size: 9415 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4409 5538 11846 12020 65 Size: 1183 3161 1129 6308 174 1396 Fragments arranged by size: 6308 3161 1396 1183 1129 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8969 10046 11995 12182 1700 Size: 110 7159 1077 1949 187 2869 Fragments arranged by size: 7159 2869 1949 1077 187 110 BsrGI T'GTAC_A Cuts at: 96 2086 10438 96 Size: 1990 8352 3009 Fragments arranged by size: 8352 3009 1990 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13351 BssSI C'ACGA_G Cuts at: 3642 6699 11411 12423 3642 Size: 3057 4712 1012 4570 Fragments arranged by size: 4712 4570 3057 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 7160 8075 9896 9899 10012 794 Size: 2088 4278 915 1821 3 113 4133 Fragments arranged by size: 4278 4133 2088 1821 915 113 3 BstBI TT'CG_AA Cuts at: 6073 6811 12155 6073 Size: 738 5344 7269 Fragments arranged by size: 7269 5344 738 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 13351 BstXI CCAn_nnnn'nTGG Cuts at: 2339 5546 5888 7884 8285 2339 Size: 3207 342 1996 401 7405 Fragments arranged by size: 7405 3207 1996 401 342 BstYI r'GATC_y Cuts at: 1972 2011 2417 3091 4227 4453 5182 6064 6667 Size: 39 406 674 1136 226 729 882 603 Cuts at: 6667 7204 7273 7726 9961 10598 10681 11048 11433 Size: 537 69 453 2235 637 83 367 385 Cuts at: 11433 11450 12902 12988 13000 13346 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2235 1977 1452 1136 882 729 674 637 603 537 453 406 385 367 346 226 86 83 69 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3039 3039 Size: 13351 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 12799 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3492 4993 487 Size: 683 56 2266 1501 8845 Fragments arranged by size: 8845 2266 1501 683 56 BtsI GCAGTG_nn' Cuts at: 7371 10010 10867 11661 11681 7371 Size: 2639 857 794 20 9041 Fragments arranged by size: 9041 2639 857 794 20 ClaI AT'CG_AT Cuts at: 1554 6141 1554 Size: 4587 8764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 13316 DraI TTT'AAA Cuts at: 7069 7237 10655 7069 Size: 168 3418 9765 Fragments arranged by size: 9765 3418 168 DraIII CAC_nnn'GTG Cuts at: 5068 7939 5068 Size: 2871 10480 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8731 12358 809 Size: 507 7415 3627 1802 Fragments arranged by size: 7415 3627 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6553 8780 10798 Size: 54 1425 50 275 18 4723 2227 2018 Cuts at: 10798 11687 13316 8 Size: 889 1629 43 Fragments arranged by size: 4723 2227 2018 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10798 1537 Size: 275 8986 4090 Fragments arranged by size: 8986 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3159 3467 3966 4197 4464 6007 6070 6126 Size: 1806 308 499 231 267 1543 63 56 Cuts at: 6126 7519 8470 8545 8557 8575 9978 11281 1353 Size: 1393 951 75 12 18 1403 1303 3423 Fragments arranged by size: 3423 1806 1543 1403 1393 1303 951 499 308 267 231 75 63 56 18 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11938 12312 12458 387 Size: 1064 138 10349 374 146 1280 Fragments arranged by size: 10349 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5422 6605 5422 Size: 1183 12168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2700 2849 3218 3660 3822 3987 4146 Size: 393 1068 149 369 442 162 165 159 Cuts at: 4146 4852 5198 5666 5846 6429 6705 8202 8292 Size: 706 346 468 180 583 276 1497 90 Cuts at: 8292 8505 8835 8940 9648 9959 10080 10371 11414 Size: 213 330 105 708 311 121 291 1043 Cuts at: 11414 12011 12798 13066 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1497 1068 1043 787 708 706 597 583 468 442 393 369 346 330 311 291 276 268 213 180 165 162 159 149 121 105 90 EcoNI CCTnn'n_nnAGG Cuts at: 2946 3233 3236 7584 8162 10059 2946 Size: 287 3 4348 578 1897 6238 Fragments arranged by size: 6238 4348 1897 578 287 3 EcoO109I rG'GnC_Cy Cuts at: 2096 3226 4730 5038 7816 2096 Size: 1130 1504 308 2778 7631 Fragments arranged by size: 7631 2778 1504 1130 308 EcoRI G'AATT_C Cuts at: 2575 3001 4003 9396 10660 11038 2575 Size: 426 1002 5393 1264 378 4888 Fragments arranged by size: 5393 4888 1264 1002 426 378 EcoRV GAT'ATC Cuts at: 6139 6139 Size: 13351 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4226 4749 5166 5324 6086 6099 7490 8087 10296 Size: 523 417 158 762 13 1391 597 2209 Cuts at: 10296 4226 Size: 7281 Fragments arranged by size: 7281 2209 1391 762 597 523 417 158 13 FspI TGC'GCA Cuts at: 11857 11857 Size: 13351 HaeII r_GCGC'y Cuts at: 1678 12498 1678 Size: 10820 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3833 3865 3878 3910 4732 Size: 32 135 32 2261 32 13 32 822 Cuts at: 4732 4764 6101 6133 6530 6532 6562 6564 7247 Size: 32 1337 32 397 2 30 2 683 Cuts at: 7247 7279 7681 7713 7765 7797 8159 8191 8267 Size: 32 402 32 52 32 362 32 76 Cuts at: 8267 8299 8393 8411 8425 8443 10448 10480 10577 Size: 32 94 18 14 18 2005 32 97 Cuts at: 10577 10595 10609 10627 11992 12024 12066 12098 1373 Size: 18 14 18 1365 32 42 32 2626 Fragments arranged by size: 2626 2261 2005 1365 1337 822 683 402 397 362 135 97 94 76 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 18 18 18 18 14 14 13 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2635 3100 3267 6201 7301 8997 Size: 988 281 697 465 167 2934 1100 1696 Cuts at: 8997 9030 9283 10695 10941 669 Size: 33 253 1412 246 3079 Fragments arranged by size: 3079 2934 1696 1412 1100 988 697 465 281 253 246 167 33 HindIII A'AGCT_T Cuts at: 748 2467 4488 7850 748 Size: 1719 2021 3362 6249 Fragments arranged by size: 6249 3362 2021 1719 HpaI GTT'AAC Cuts at: 2635 10941 2635 Size: 8306 5045 KpnI G_GTAC'C Cuts at: 6992 10676 6992 Size: 3684 9667 MfeI C'AATT_G Cuts at: 4037 4074 4624 4968 6177 10950 4037 Size: 37 550 344 1209 4773 6438 Fragments arranged by size: 6438 4773 1209 550 344 37 MluI A'CGCG_T Cuts at: 13227 13227 Size: 13351 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2207 6704 7667 8790 9655 10433 12465 12649 Size: 902 4497 963 1123 865 778 2032 184 Cuts at: 12649 1305 Size: 2007 Fragments arranged by size: 4497 2032 2007 1123 963 902 865 778 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 8782 10 Size: 54 1425 343 6950 4579 Fragments arranged by size: 6950 4579 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3126 3258 3423 7077 7679 11309 11668 Size: 648 1960 132 165 3654 602 3630 359 Cuts at: 11668 11827 518 Size: 159 2042 Fragments arranged by size: 3654 3630 2042 1960 648 602 359 165 159 132 MspA1I CmG'CkG Cuts at: 1606 2273 5507 5813 9896 10313 10319 10767 11444 Size: 667 3234 306 4083 417 6 448 677 Cuts at: 11444 12592 12837 1606 Size: 1148 245 2120 Fragments arranged by size: 4083 3234 2120 1148 677 667 448 417 306 245 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 12799 NdeI CA'TA_TG Cuts at: 387 4417 5938 6662 387 Size: 4030 1521 724 7076 Fragments arranged by size: 7076 4030 1521 724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 13351 NotI GC'GGCC_GC Cuts at: 10798 10798 Size: 13351 NsiI A_TGCA'T Cuts at: 2083 2457 2662 2083 Size: 374 205 12772 Fragments arranged by size: 12772 374 205 NspI r_CATG'y Cuts at: 1132 1229 2282 3759 5823 9799 10709 12254 1132 Size: 97 1053 1477 2064 3976 910 1545 2229 Fragments arranged by size: 3976 2229 2064 1545 1477 1053 910 97 PasI CC'CwG_GG Cuts at: 1348 2567 6741 1348 Size: 1219 4174 7958 Fragments arranged by size: 7958 4174 1219 PciI A'CATG_T Cuts at: 2278 3755 5819 12250 2278 Size: 1477 2064 6431 3379 Fragments arranged by size: 6431 3379 2064 1477 PflMI CCAn_nnn'nTGG Cuts at: 1108 6270 8249 9071 9203 1108 Size: 5162 1979 822 132 5256 Fragments arranged by size: 5256 5162 1979 822 132 PmeI GTTT'AAAC Cuts at: 10655 10655 Size: 13351 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3466 3498 9795 9827 10225 10257 11420 11452 12933 Size: 32 6297 32 398 32 1163 32 1481 Cuts at: 12933 12965 3466 Size: 32 3852 Fragments arranged by size: 6297 3852 1481 1163 398 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 3226 4730 2096 Size: 1130 1504 10717 Fragments arranged by size: 10717 1504 1130 PshAI GACnn'nnGTC Cuts at: 1613 6196 1613 Size: 4583 8768 PsiI TTA'TAA Cuts at: 5453 10921 5453 Size: 5468 7883 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4017 4049 4017 Size: 32 13319 PstI C_TGCA'G Cuts at: 830 2878 5812 10318 10703 830 Size: 2048 2934 4506 385 3478 Fragments arranged by size: 4506 3478 2934 2048 385 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13351 PvuII CAG'CTG Cuts at: 2273 5507 5813 9896 10313 10319 2273 Size: 3234 306 4083 417 6 5305 Fragments arranged by size: 5305 4083 3234 417 306 6 SacI G_AGCT'C Cuts at: 5424 6607 5424 Size: 1183 12168 SalI G'TCGA_C Cuts at: 1936 10693 1936 Size: 8757 4594 SapI GCTCTTCn'nnn_ Cuts at: 3467 4197 3467 Size: 730 12621 SbfI CC_TGCA'GG Cuts at: 10703 10703 Size: 13351 ScaI AGT'ACT Cuts at: 4429 8632 11599 13311 4429 Size: 4203 2967 1712 4469 Fragments arranged by size: 4469 4203 2967 1712 SexAI A'CCwGG_T Cuts at: 4018 4018 Size: 13351 SfcI C'TryA_G Cuts at: 826 1045 2874 5724 5808 5914 6707 8005 8051 Size: 219 1829 2850 84 106 793 1298 46 Cuts at: 8051 8928 9237 10314 10476 10699 11834 12515 12706 Size: 877 309 1077 162 223 1135 681 191 Cuts at: 12706 826 Size: 1471 Fragments arranged by size: 2850 1829 1471 1298 1135 1077 877 793 681 309 223 219 191 162 106 84 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13351 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13351 SmaI CCC'GGG Cuts at: 1872 9510 10678 1872 Size: 7638 1168 4545 Fragments arranged by size: 7638 4545 1168 SmlI C'TyrA_G Cuts at: 820 1017 1943 5140 9390 9497 11455 12356 12618 Size: 197 926 3197 4250 107 1958 901 262 Cuts at: 12618 820 Size: 1553 Fragments arranged by size: 4250 3197 1958 1553 926 901 262 197 107 SnaBI TAC'GTA Cuts at: 493 11142 493 Size: 10649 2702 SpeI A'CTAG_T Cuts at: 152 2107 152 Size: 1955 11396 SphI G_CATG'C Cuts at: 1132 10709 1132 Size: 9577 3774 SspI AAT'ATT Cuts at: 5 52 3021 3637 4534 5317 7661 7735 11275 Size: 47 2969 616 897 783 2344 74 3540 Cuts at: 11275 5 Size: 2081 Fragments arranged by size: 3540 2969 2344 2081 897 783 616 74 47 StuI AGG'CCT Cuts at: 1517 4498 6723 9980 10392 1517 Size: 2981 2225 3257 412 4476 Fragments arranged by size: 4476 3257 2981 2225 412 StyI C'CwwG_G Cuts at: 513 1065 6210 8099 9202 10790 513 Size: 552 5145 1889 1103 1588 3074 Fragments arranged by size: 5145 3074 1889 1588 1103 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 2655 5973 6510 11378 11395 11548 Size: 1255 537 538 3318 537 4868 17 153 Cuts at: 11548 11733 12178 325 Size: 185 445 1498 Fragments arranged by size: 4868 3318 1498 1255 538 537 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2086 2525 4427 Size: 274 80 33 51 1443 109 439 1902 Cuts at: 4427 4863 8630 10438 10607 11597 13309 96 Size: 436 3767 1808 169 990 1712 138 Fragments arranged by size: 3767 1902 1808 1712 1443 990 439 436 274 169 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6439 6471 7462 7494 13311 13343 6439 Size: 32 991 32 5817 32 6447 Fragments arranged by size: 6447 5817 991 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 13351 XbaI T'CTAG_A Cuts at: 4097 8128 10687 4097 Size: 4031 2559 6761 Fragments arranged by size: 6761 4031 2559 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5971 7717 8285 8905 5971 Size: 1746 568 620 10417 Fragments arranged by size: 10417 1746 620 568 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 13351 XmaI C'CCGG_G Cuts at: 1870 9508 10676 1870 Size: 7638 1168 4545 Fragments arranged by size: 7638 4545 1168 XmnI GAAnn'nnTTC Cuts at: 2929 3663 4710 4938 6807 9639 9807 11480 2929 Size: 734 1047 228 1869 2832 168 1673 4800 Fragments arranged by size: 4800 2832 1869 1673 1047 734 228 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 11159 276 Size: 53 83 186 936 9625 2468 Fragments arranged by size: 9625 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BplI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI