Restriction map for
pFN21AE3215
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MspA1I MspI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 125 CGCG CG^CG
AflIII 738 ACATGT A^C[GA][CT]GT
AlwNI 938 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 43 GGATCC G^GATCC
BanII 1317 GAGCCC G[GA]GC[CT]^C
BfaI 914 CTAG C^TAG
BsaBI 282 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 823 CGATCG CG[GA][CT]^CG
Bsp1407I 472 TGTACA T^GTACA
BspHI 713 TCATGA T^CATGA
BspLU11I 738 ACATGT A^CATGT
BsrGI 472 TGTACA T^GTACA
BssSI 476 CACGAG CACGAG(-5/-1)
BstAPI 735 GCAACATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 125 CGCG CG^CG
BstYI 43 GGATCC [GA]^GATC[CT]
DraII 1212 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 167 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 803 CCATGG C^C[GA][CT]GG
EarI 487 CTCTTC CTCTTC(1/4)
EcoNI 1204 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1212 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 74 GAATTC G^AATTC
HincII 971 GTCAAC GT[CT]^[GA]AC
HindII 971 GTCAAC GT[CT]^[GA]AC
Ksp632I 487 CTCTTC CTCTTC(1/4)
MaeI 914 CTAG C^TAG
MamI 282 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 43 GGATCC [GA]^GATC[CT]
MvnI 125 CGCG CG^CG
NcoI 803 CCATGG C^CATGG
PflMI 1420 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1212 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1082 CTGCAG CTGCA^G
PvuI 823 CGATCG CGAT^CG
RcaI 713 TCATGA T^CATGA
SexAI 168 ACCTGGT A^CC[AT]GGT
SspBI 472 TGTACA T^GTACA
TaqI 822 TCGA T^CGA
Tsp45I 326 GTGAC ^GT[GC]AC
TthHB8I 822 TCGA T^CGA
Van91I 1420 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 567 CCATATGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 43 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 74 GAATTC [GA]^AATT[CT]
903 AAATTC
AfaI 473 GTAC GT^AC
1088 GTAC
ApoI 74 GAATTC [GA]^AATT[CT]
903 AAATTC
Asp700I 607 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
838 GAAACCTTTC
AspHI 751 GAGCAC G[AT]GC[AT]^C
782 GTGCTC
BpmI 788 CTCCAG CTGGAG(16/14)
1208 CTCCAG
BsiHKAI 751 GAGCAC G[AT]GC[AT]^C
782 GTGCTC
BsmAI 539 GAGAC GTCTC(1/5)
531 GTCTC
BsmFI 272 GGGAC GGGAC(10/14)
883 GTCCC
EcoT14I 47 CCTTGG C^C[AT][AT]GG
803 CCATGG
HaeIII 1361 GGCC GG^CC
1376 GGCC
MseI 347 TTAA T^TAA
874 TTAA
MslI 319 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CATCATGATG
NdeI 568 CATATG CA^TATG
682 CATATG
NspI 378 GCATGT [GA]CATG^[CT]
738 ACATGT
PleI 898 GACTC GAGTC(4/5)
1347 GAGTC
RsaI 473 GTAC GT^AC
1088 GTAC
SfcI 441 CTGTAG C^T[GA][CT]AG
1082 CTGCAG
StyI 47 CCTTGG C^C[AT][AT]GG
803 CCATGG
TfiI 366 GATTC G^A[AT]TC
1197 GAATC
Tru9I 347 TTAA T^TAA
874 TTAA
XmnI 607 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
838 GAAACCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BsaJI BseRI BsgI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI Tsp509I TspRI