(Circular) (Six-base) MAPSORT of: pFN21AE3215.seq Check: 9898 from: 1 to: 6178 pFN21AE3215 6178 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2956 2956 Size: 6178 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3521 1937 Size: 1584 4594 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3324 4241 5625 5893 1632 Size: 1692 917 1384 268 1917 Fragments arranged by size: 1917 1692 1384 917 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 2743 5077 6054 1203 Size: 1540 2334 977 1327 Fragments arranged by size: 2334 1540 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3042 1184 Size: 1858 4320 AlwNI CAG_nnn'CTG Cuts at: 2948 3921 5493 2948 Size: 973 1572 3633 Fragments arranged by size: 3633 1572 973 ApaLI G'TGCA_C Cuts at: 4235 5391 4235 Size: 1156 5022 ApoI r'AATT_y Cuts at: 1493 2079 2908 3487 3713 3865 5974 1493 Size: 586 829 579 226 152 2109 1697 Fragments arranged by size: 2109 1697 829 586 579 226 152 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 3503 1870 Size: 73 1560 4545 Fragments arranged by size: 4545 1560 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2304 2337 2304 Size: 33 6145 BamHI G'GATC_C Cuts at: 2048 3508 3875 2048 Size: 1460 367 4351 Fragments arranged by size: 4351 1460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3499 4954 618 Size: 325 217 627 1712 1455 1842 Fragments arranged by size: 1842 1712 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3326 3497 1391 Size: 1935 171 4072 Fragments arranged by size: 4072 1935 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4789 136 Size: 107 122 71 631 605 3117 1525 Fragments arranged by size: 3117 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6173 6173 Size: 6178 BlpI GC'TnA_GC Cuts at: 3595 3595 Size: 6178 Bme1580I G_kGCm'C Cuts at: 1790 4239 5395 1790 Size: 2449 1156 2573 Fragments arranged by size: 2573 2449 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3289 3909 4867 Size: 416 53 83 399 24 1872 620 958 Cuts at: 4867 442 Size: 1753 Fragments arranged by size: 1872 1753 958 620 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2507 2507 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2778 3198 4838 1239 Size: 1539 420 1640 2579 Fragments arranged by size: 2579 1640 1539 420 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 6178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4303 5168 5466 4303 Size: 865 298 5015 Fragments arranged by size: 5015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4841 882 Size: 617 3342 2219 Fragments arranged by size: 3342 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3969 493 Size: 711 2765 2702 Fragments arranged by size: 2765 2702 711 BsaBI GATnn'nnATC Cuts at: 2291 6172 2291 Size: 3881 2297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4611 5009 5283 5430 5841 4611 Size: 398 274 147 411 4948 Fragments arranged by size: 4948 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3127 3157 1178 Size: 30 165 30 1724 30 4199 Fragments arranged by size: 4199 1724 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2781 3033 3087 3201 3255 1186 Size: 1595 252 54 114 54 4109 Fragments arranged by size: 4109 1595 252 114 54 54 BseYI C'CCAG_C Cuts at: 2941 5381 5877 2941 Size: 2440 496 3242 Fragments arranged by size: 3242 2440 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2109 2480 3240 3354 1163 Size: 573 373 371 760 114 3987 Fragments arranged by size: 3987 760 573 373 371 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2831 3628 4389 5417 1540 Size: 275 186 830 797 761 1028 2301 Fragments arranged by size: 2301 1028 830 797 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2760 2791 4239 4324 5395 2760 Size: 31 1448 85 1071 3543 Fragments arranged by size: 3543 1448 1071 85 31 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2760 2791 3326 3497 4239 4324 5395 Size: 399 970 31 535 171 742 85 1071 Cuts at: 5395 1391 Size: 2174 Fragments arranged by size: 2174 1071 970 742 535 399 171 85 31 BspEI T'CCGG_A Cuts at: 5841 5841 Size: 6178 BspHI T'CATG_A Cuts at: 2718 4067 5797 2718 Size: 1349 1730 3099 Fragments arranged by size: 3099 1730 1349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 2956 3080 3533 844 Size: 1287 825 124 453 3489 Fragments arranged by size: 3489 1287 825 453 124 BsrBI CCG'CTC Cuts at: 1394 1823 4065 1394 Size: 429 2242 3507 Fragments arranged by size: 3507 2242 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4673 4847 65 Size: 1183 3425 174 1396 Fragments arranged by size: 3425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 5009 1700 Size: 110 3012 187 2869 Fragments arranged by size: 3012 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2477 96 Size: 2381 3797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 2481 4238 5250 2481 Size: 1757 1012 3409 Fragments arranged by size: 3409 1757 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2746 794 Size: 1952 4226 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6178 BstYI r'GATC_y Cuts at: 1972 2048 3508 3875 4260 4277 5729 5815 5827 Size: 76 1460 367 385 17 1452 86 12 Cuts at: 5827 6173 1972 Size: 346 1977 Fragments arranged by size: 1977 1460 1452 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2808 513 Size: 552 939 804 3883 Fragments arranged by size: 3883 939 804 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2552 487 Size: 683 56 1326 4113 Fragments arranged by size: 4113 1326 683 56 BtsI GCAGTG_nn' Cuts at: 2480 3694 4488 4508 2480 Size: 1214 794 20 4150 Fragments arranged by size: 4150 1214 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2718 2753 529 Size: 35 2154 35 3954 Fragments arranged by size: 3954 2154 35 35 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DraIII CAC_nnn'GTG Cuts at: 2177 2177 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3625 4514 6143 Size: 54 1425 50 275 18 1795 889 1629 Cuts at: 6143 8 Size: 43 Fragments arranged by size: 1795 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 4108 1353 Size: 1145 1610 3423 Fragments arranged by size: 3423 1610 1145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2691 3399 4765 5139 5285 387 Size: 1064 138 1102 708 1366 374 146 1280 Fragments arranged by size: 1366 1280 1102 1064 708 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2778 3198 3324 4241 4838 5625 5893 Size: 393 1146 420 126 917 597 787 268 Cuts at: 5893 1239 Size: 1524 Fragments arranged by size: 1524 1146 917 787 597 420 393 268 126 EcoNI CCTnn'n_nnAGG Cuts at: 3213 3213 Size: 6178 EcoO109I rG'GnC_Cy Cuts at: 3218 3218 Size: 6178 EcoRI G'AATT_C Cuts at: 2079 3487 3865 2079 Size: 1408 378 4392 Fragments arranged by size: 4392 1408 378 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 5325 1678 Size: 3647 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3127 3159 4819 4851 4893 Size: 32 135 32 1555 32 1660 32 42 Cuts at: 4893 4925 1373 Size: 32 2626 Fragments arranged by size: 2626 1660 1555 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2978 3522 3768 669 Size: 988 281 1040 544 246 3079 Fragments arranged by size: 3079 1040 988 544 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6178 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5292 5476 1305 Size: 3987 184 2007 Fragments arranged by size: 3987 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4356 Fragments arranged by size: 4356 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2720 4136 4495 4654 518 Size: 648 1162 392 1416 359 159 2042 Fragments arranged by size: 2042 1416 1162 648 392 359 159 MspA1I CmG'CkG Cuts at: 1606 3594 4271 5419 5664 1606 Size: 1988 677 1148 245 2120 Fragments arranged by size: 2120 1988 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2808 513 Size: 552 939 804 3883 Fragments arranged by size: 3883 939 804 552 NdeI CA'TA_TG Cuts at: 387 2574 2688 387 Size: 2187 114 3877 Fragments arranged by size: 3877 2187 114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2387 2747 3536 5081 1132 Size: 97 1158 360 789 1545 2229 Fragments arranged by size: 2229 1545 1158 789 360 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 2743 5077 2743 Size: 2334 3844 PflMI CCAn_nnn'nTGG Cuts at: 1108 3431 1108 Size: 2323 3855 PfoI T'CCnGG_A Cuts at: 2260 3214 3430 2260 Size: 954 216 5008 Fragments arranged by size: 5008 954 216 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3218 3218 Size: 6178 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6178 PsiI TTA'TAA Cuts at: 2199 2666 3276 3748 2199 Size: 467 610 472 4629 Fragments arranged by size: 4629 610 472 467 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2470 2502 2470 Size: 32 6146 PstI C_TGCA'G Cuts at: 830 3091 3530 830 Size: 2261 439 3478 Fragments arranged by size: 3478 2261 439 PvuI CG_AT'CG Cuts at: 2001 2831 2001 Size: 830 5348 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 4426 6138 4426 Size: 1712 4466 SexAI A'CCwGG_T Cuts at: 2173 2173 Size: 6178 SfcI C'TryA_G Cuts at: 826 1045 2446 3087 3526 4661 5342 5533 826 Size: 219 1401 641 439 1135 681 191 1471 Fragments arranged by size: 1471 1401 1135 681 641 439 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 3505 1872 Size: 1633 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4282 5183 5445 820 Size: 197 926 2339 901 262 1553 Fragments arranged by size: 2339 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6178 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2808 3617 513 Size: 552 939 48 756 809 3074 Fragments arranged by size: 3074 939 809 756 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2821 4205 4222 4375 4560 5005 Size: 1255 465 776 1384 17 153 185 445 Cuts at: 5005 325 Size: 1498 Fragments arranged by size: 1498 1384 1255 776 465 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2477 4424 6136 Size: 274 80 33 51 1443 500 1947 1712 Cuts at: 6136 96 Size: 138 Fragments arranged by size: 1947 1712 1443 500 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6138 6170 6138 Size: 32 6146 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6178 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 6178 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 3503 1870 Size: 1633 4545 XmnI GAAnn'nnTTC Cuts at: 2616 2847 4307 2616 Size: 231 1460 4487 Fragments arranged by size: 4487 1460 231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI