Restriction map for
pFN21AE9652
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# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AvrII BbeI BbrPI BbvCI
BcgI BlnI BsiEI BsiWI BspDI
BspLU11I BssHII BstBI ClaI CpoI
EagI EclXI Eco52I EcoT22I FseI
KasI KpnI MluI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SfiI
SfoI SfuI SgrAI SpeI SphI
StuI SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 4655 TCCGGA T^CCGGA
AflIII 7334 ACACGT A^C[GA][CT]GT
AhdI 4440 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 7436 GTGCAC G^TGCAC
Aor51HI 2096 AGCGCT AGC^GCT
ApaLI 7436 GTGCAC G^TGCAC
AseI 3646 ATTAAT AT^TAAT
AsnI 3646 ATTAAT AT^TAAT
AspEI 4440 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3272 TGCGCA TGC^GCA
BclI 2768 TGATCA T^GATCA
BlpI 559 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 559 GCTCAGC GC^T[ACGT]AGC
BsaAI 2167 TACGTA [CT]AC^GT[GA]
BsaWI 4655 TCCGGA [AT]^CCGG[AT]
BseAI 4655 TCCGGA T^CCGGA
BsmBI 1719 GAGACG CGTCTC(1/5)
Bsp1407I 1131 TGTACA T^GTACA
BspEI 4655 TCCGGA T^CCGGA
BspHI 3360 TCATGA T^CATGA
BsrGI 1131 TGTACA T^GTACA
Bst1107I 5964 GTATAC GTA^TAC
BstEII 5187 GGTGACC G^GT[ACGT]ACC
BstPI 5187 GGTGACC G^GT[ACGT]ACC
BstZ17I 5964 GTATAC GTA^TAC
CelII 559 GCTCAGC GC^T[ACGT]AGC
Eam1105I 4440 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2096 AGCGCT AGC^GCT
EcoO65I 5187 GGTGACC G^GT[ACGT]ACC
EcoRV 4633 GATATC GAT^ATC
FbaI 2768 TGATCA T^GATCA
FspI 3272 TGCGCA TGC^GCA
HpaI 6786 GTTAAC GTT^AAC
KspI 1422 CCGCGG CCGC^GG
MfeI 5000 CAATTG C^AATTG
MroI 4655 TCCGGA T^CCGGA
MunI 5000 CAATTG C^AATTG
NaeI 3394 GCCGGC GCC^GGC
NgoMIV 3394 GCCGGC G^CCGGC
PshBI 3646 ATTAAT AT^TAAT
RcaI 3360 TCATGA T^CATGA
SacI 1966 GAGCTC GAGCT^C
SacII 1422 CCGCGG CCGC^GG
ScaI 5534 AGTACT AGT^ACT
SmaI 3626 CCCGGG CCC^GGG
SnaBI 2167 TACGTA TAC^GTA
SspBI 1131 TGTACA T^GTACA
SspI 4495 AATATT AAT^ATT
XbaI 7165 TCTAGA T^CTAGA
XmaCI 3626 CCCGGG C^CCGGG
XmaI 3626 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 1423 CGCG CG^CG
4214 CGCG
AflII 484 CTTAAG C^TTAAG
3073 CTTAAG
AocI 4758 CCTGAGG CC^T[ACGT]AGG
7606 CCTGAGG
ApaI 796 GGGCCC GGGCC^C
7253 GGGCCC
AspI 1119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6493 GACCTCGTC
BamHI 4652 GGATCC G^GATCC
6941 GGATCC
BciVI 2078 GGATAC GTATCC(6/5)
6133 GTATCC
BfrI 484 CTTAAG C^TTAAG
3073 CTTAAG
BsrBI 6330 CCGCTC CCGCTC(-3/-3)
4282 GAGCGG
BstUI 1423 CGCG CG^CG
4214 CGCG
Bsu36I 4758 CCTGAGG CC^T[ACGT]AGG
7606 CCTGAGG
DraIII 2911 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6082 CACGAGGTG
Eco81I 4758 CCTGAGG CC^T[ACGT]AGG
7606 CCTGAGG
HaeII 2096 AGCGCT [GA]GCGC^[CT]
5681 GGCGCT
HincII 5053 GTTGAC GT[CT]^[GA]AC
6786 GTTAAC
HindII 5053 GTTGAC GT[CT]^[GA]AC
6786 GTTAAC
MvnI 1423 CGCG CG^CG
4214 CGCG
NdeI 3932 CATATG CA^TATG
4827 CATATG
NspI 909 GCATGT [GA]CATG^[CT]
3239 ACATGC
PaeR7I 1442 CTCGAG C^TCGAG
1688 CTCGAG
PflFI 1119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6493 GACCTCGTC
SbfI 3372 CCTGCAGG CCTGCA^GG
3708 CCTGCAGG
Sse8387I 3372 CCTGCAGG CCTGCA^GG
3708 CCTGCAGG
Tth111I 1119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6493 GACCTCGTC
XcmI 3812 CCAGCCTCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4861 CCAACAACTTGGTGG
XhoI 1442 CTCGAG C^TCGAG
1688 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI