(Circular) (Six-base) MAPSORT of: pFN21AE9652.seq Check: 8846 from: 1 to: 12448 pFN21AE9652 12448 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3022 5385 3022 Size: 2363 10085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10258 278 Size: 53 83 186 936 8722 2468 Fragments arranged by size: 8722 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2932 3936 7436 7970 9462 9791 1937 Size: 995 1004 3500 534 1492 329 4594 Fragments arranged by size: 4594 3500 1492 1004 995 534 329 Acc65I G'GTAC_C Cuts at: 9769 9769 Size: 12448 AclI AA'CG_TT Cuts at: 1563 10575 10948 1563 Size: 9012 373 3063 Fragments arranged by size: 9012 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2831 4014 5337 5572 6060 10511 11895 12163 Size: 1199 1183 1323 235 488 4451 1384 268 Cuts at: 12163 1632 Size: 1917 Fragments arranged by size: 4451 1917 1384 1323 1199 1183 488 268 235 AfeI AGC'GCT Cuts at: 4103 4103 Size: 12448 AflII C'TTAA_G Cuts at: 820 1017 2489 5078 820 Size: 197 1472 2589 8190 Fragments arranged by size: 8190 2589 1472 197 AflIII A'CryG_T Cuts at: 1203 9339 11347 12324 1203 Size: 8136 2008 977 1327 Fragments arranged by size: 8136 2008 1327 977 AgeI A'CCGG_T Cuts at: 11279 11279 Size: 12448 AhdI GACnn_n'nnGTC Cuts at: 6450 11177 6450 Size: 4727 7721 AleI CACnn'nnGTG Cuts at: 1166 4664 6486 9393 1166 Size: 3498 1822 2907 4221 Fragments arranged by size: 4221 3498 2907 1822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4401 7868 8609 1184 Size: 3217 3467 741 5023 Fragments arranged by size: 5023 3467 3217 741 AlwNI CAG_nnn'CTG Cuts at: 2516 2572 2777 5666 7595 10191 11763 2516 Size: 56 205 2889 1929 2596 1572 3201 Fragments arranged by size: 3201 2889 2596 1929 1572 205 56 ApaI G_GGCC'C Cuts at: 2805 9262 2805 Size: 6457 5991 ApaLI G'TGCA_C Cuts at: 9441 10505 11661 9441 Size: 1064 1156 10228 Fragments arranged by size: 10228 1156 1064 ApoI r'AATT_y Cuts at: 1493 2041 2182 2535 4004 4325 4634 5206 5336 Size: 548 141 353 1469 321 309 572 130 Cuts at: 5336 6257 8571 9757 9983 10135 12244 1493 Size: 921 2314 1186 226 152 2109 1697 Fragments arranged by size: 2314 2109 1697 1469 1186 921 572 548 353 321 309 226 152 141 130 AseI AT'TA_AT Cuts at: 160 1032 5652 11002 160 Size: 872 4620 5350 1606 Fragments arranged by size: 5350 4620 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12448 AvaI C'yCGr_G Cuts at: 1870 1943 3447 3693 5631 8030 9773 1870 Size: 73 1504 246 1938 2399 1743 4545 Fragments arranged by size: 4545 2399 1938 1743 1504 246 73 BamHI G'GATC_C Cuts at: 6657 8946 9778 10145 6657 Size: 2289 832 367 8960 Fragments arranged by size: 8960 2289 832 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2519 3572 4347 5128 9769 Size: 325 217 627 732 1053 775 781 4641 Cuts at: 9769 11224 618 Size: 1455 1842 Fragments arranged by size: 4641 1842 1455 1053 781 775 732 627 325 217 BanII G_rGCy'C Cuts at: 1391 2805 3252 3421 3975 4158 4233 7232 7635 Size: 1414 447 169 554 183 75 2999 403 Cuts at: 7635 9262 9544 9767 1391 Size: 1627 282 223 4072 Fragments arranged by size: 4072 2999 1627 1414 554 447 403 282 223 183 169 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5104 5608 6467 928 Size: 971 3205 504 859 6909 Fragments arranged by size: 6909 3205 971 859 504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10639 10673 1903 Size: 34 8702 34 3678 Fragments arranged by size: 8702 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4077 8149 10340 11550 4077 Size: 4072 2191 1210 4975 Fragments arranged by size: 4975 4072 2191 1210 BclI T'GATC_A Cuts at: 1548 4773 1548 Size: 3225 9223 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2528 6143 7786 Size: 107 122 71 631 605 856 3615 1643 Cuts at: 7786 11059 136 Size: 3273 1525 Fragments arranged by size: 3615 3273 1643 1525 856 631 605 122 107 71 BglII A'GATC_T Cuts at: 5917 6000 7161 12443 5917 Size: 83 1161 5282 5922 Fragments arranged by size: 5922 5282 1161 83 BlpI GC'TnA_GC Cuts at: 2565 9865 2565 Size: 7300 5148 Bme1580I G_kGCm'C Cuts at: 1790 2524 2805 3427 9262 9445 10509 11665 1790 Size: 734 281 622 5835 183 1064 1156 2573 Fragments arranged by size: 5835 2573 1156 1064 734 622 281 183 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 4633 6836 Size: 416 53 83 399 24 2489 727 2203 Cuts at: 6836 8049 8710 9327 10179 11137 442 Size: 1213 661 617 852 958 1753 Fragments arranged by size: 2489 2203 1753 1213 958 852 727 661 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12448 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7180 7669 7180 Size: 489 11959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2241 2247 2422 2469 2738 2861 2913 6878 Size: 1002 6 175 47 269 123 52 3965 Cuts at: 6878 7617 8081 8213 8260 8266 8622 8640 8912 Size: 739 464 132 47 6 356 18 272 Cuts at: 8912 8957 9035 9451 9712 11108 1239 Size: 45 78 416 261 1396 2579 Fragments arranged by size: 3965 2579 1396 1002 739 464 416 356 272 269 261 175 132 123 78 52 47 47 45 18 6 6 Bpu10I CC'TnA_GC Cuts at: 2390 3834 4809 6063 8424 2390 Size: 1444 975 1254 2361 6414 Fragments arranged by size: 6414 2361 1444 1254 975 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2964 4880 5006 5024 5807 8248 9556 10573 11438 Size: 1916 126 18 783 2441 1308 1017 865 Cuts at: 11438 11736 2964 Size: 298 3676 Fragments arranged by size: 3676 2441 1916 1308 1017 865 783 298 126 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6437 6777 8933 9327 11111 882 Size: 617 4938 340 2156 394 1784 2219 Fragments arranged by size: 4938 2219 2156 1784 617 394 340 BsaAI yAC'GTr Cuts at: 493 1204 4174 10239 493 Size: 711 2970 6065 2702 Fragments arranged by size: 6065 2970 2702 711 BsaBI GATnn'nnATC Cuts at: 2483 7422 8841 12442 2483 Size: 4939 1419 3601 2489 Fragments arranged by size: 4939 3601 2489 1419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2345 4738 7255 10255 Size: 53 83 186 936 812 2393 2517 3000 Cuts at: 10255 10637 11272 275 Size: 382 635 1451 Fragments arranged by size: 3000 2517 2393 1451 936 812 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6660 10881 11279 11553 11700 12111 6660 Size: 4221 398 274 147 411 6997 Fragments arranged by size: 6997 4221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3598 3628 8885 8915 9008 Size: 30 165 30 2195 30 5257 30 93 Cuts at: 9008 9038 9710 9740 1178 Size: 30 672 30 3886 Fragments arranged by size: 5257 3886 2195 672 165 93 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2425 2497 3306 3503 3557 5533 7172 7658 Size: 1239 72 809 197 54 1976 1639 486 Cuts at: 7658 8048 8696 9629 1186 Size: 390 648 933 4005 Fragments arranged by size: 4005 1976 1639 1239 933 809 648 486 390 197 72 54 BseYI C'CCAG_C Cuts at: 2726 3096 3114 3473 3524 6532 6958 7588 11651 Size: 370 18 359 51 3008 426 630 4063 Cuts at: 11651 12147 2726 Size: 496 3027 Fragments arranged by size: 4063 3027 3008 630 496 426 370 359 51 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3056 3666 7943 8685 1163 Size: 573 1320 610 4277 742 4926 Fragments arranged by size: 4926 4277 1320 742 610 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9898 10659 11687 1540 Size: 275 186 7897 761 1028 2301 Fragments arranged by size: 7897 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3975 4262 7921 8088 9445 10509 10594 11665 3975 Size: 287 3659 167 1357 1064 85 1071 4758 Fragments arranged by size: 4758 3659 1357 1071 1064 287 167 85 BsmI GAATG_Cn' Cuts at: 2995 3714 6756 9959 10052 2995 Size: 719 3042 3203 93 5391 Fragments arranged by size: 5391 3203 3042 719 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3718 3718 Size: 12448 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2524 2805 3252 3421 3427 3975 4158 Size: 399 734 281 447 169 6 548 183 Cuts at: 4158 4233 4262 7232 7635 7921 8088 9262 9445 Size: 75 29 2970 403 286 167 1174 183 Cuts at: 9445 9544 9767 10509 10594 11665 1391 Size: 99 223 742 85 1071 2174 Fragments arranged by size: 2970 2174 1174 1071 742 734 548 447 403 399 286 281 223 183 183 169 167 99 85 75 29 6 BspEI T'CCGG_A Cuts at: 6660 12111 6660 Size: 5451 6997 BspHI T'CATG_A Cuts at: 5365 10337 12067 5365 Size: 4972 1730 5746 Fragments arranged by size: 5746 4972 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3022 5222 5385 5681 5721 9803 844 Size: 2178 2200 163 296 40 4082 3489 Fragments arranged by size: 4082 3489 2200 2178 296 163 40 BsrBI CCG'CTC Cuts at: 1394 1823 6289 8337 10335 1394 Size: 429 4466 2048 1998 3507 Fragments arranged by size: 4466 3507 2048 1998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3408 4542 4596 4940 10943 11117 65 Size: 1183 2160 1134 54 344 6003 174 1396 Fragments arranged by size: 6003 2160 1396 1183 1134 344 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 2624 5399 7326 11092 11279 1700 Size: 110 814 2775 1927 3766 187 2869 Fragments arranged by size: 3766 2869 2775 1927 814 187 110 BsrGI T'GTAC_A Cuts at: 96 3136 96 Size: 3040 9408 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12448 BssSI C'ACGA_G Cuts at: 6464 6594 8087 10508 11520 6464 Size: 130 1493 2421 1012 7392 Fragments arranged by size: 7392 2421 1493 1012 130 BstAPI GCAn_nnn'nTGC Cuts at: 794 2920 3311 5286 6339 794 Size: 2126 391 1975 1053 6903 Fragments arranged by size: 6903 2126 1975 1053 391 BstBI TT'CG_AA Cuts at: 11252 11252 Size: 12448 BstEII G'GTnAC_C Cuts at: 1184 7192 1184 Size: 6008 6440 BstXI CCAn_nnnn'nTGG Cuts at: 2162 3283 6873 2162 Size: 1121 3590 7737 Fragments arranged by size: 7737 3590 1121 BstYI r'GATC_y Cuts at: 1972 2023 2385 5035 5917 6000 6160 6657 7081 Size: 51 362 2650 882 83 160 497 424 Cuts at: 7081 7161 8946 9778 10145 10530 10547 11999 12085 Size: 80 1785 832 367 385 17 1452 86 Cuts at: 12085 12097 12443 1972 Size: 12 346 1977 Fragments arranged by size: 2650 1977 1785 1452 882 832 497 424 385 367 362 346 160 86 83 80 51 17 12 BstZ17I GTA'TAC Cuts at: 7971 7971 Size: 12448 Bsu36I CC'TnA_GG Cuts at: 6764 9612 6764 Size: 2848 9600 BtgI C'CryG_G Cuts at: 513 1065 2004 3427 4579 4672 5385 6487 7546 Size: 552 939 1423 1152 93 713 1102 1059 Cuts at: 7546 7822 8673 513 Size: 276 851 4288 Fragments arranged by size: 4288 1423 1152 1102 1059 939 851 713 552 276 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3066 487 Size: 683 56 1840 9869 Fragments arranged by size: 9869 1840 683 56 BtsI GCAGTG_nn' Cuts at: 7952 7980 7991 9400 9602 9964 10758 10778 7952 Size: 28 11 1409 202 362 794 20 9622 Fragments arranged by size: 9622 1409 794 362 202 28 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12413 DraI TTT'AAA Cuts at: 4330 6919 8076 9752 4330 Size: 2589 1157 1676 7026 Fragments arranged by size: 7026 2589 1676 1157 DraIII CAC_nnn'GTG Cuts at: 4921 8092 4921 Size: 3171 9277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5612 6312 8639 11455 809 Size: 507 4296 700 2327 2816 1802 Fragments arranged by size: 4296 2816 2327 1802 700 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 3482 4683 Size: 54 1425 50 275 18 846 806 1201 Cuts at: 4683 5449 6290 6985 7557 8097 9895 10784 12413 Size: 766 841 695 572 540 1798 889 1629 Cuts at: 12413 8 Size: 43 Fragments arranged by size: 1798 1629 1425 1201 889 846 841 806 766 695 572 540 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9895 1537 Size: 275 8083 4090 Fragments arranged by size: 8083 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3232 3247 3512 3551 3626 3716 3798 4146 Size: 1879 15 265 39 75 90 82 348 Cuts at: 4146 5979 8307 8349 8904 9562 10378 1353 Size: 1833 2328 42 555 658 816 3423 Fragments arranged by size: 3423 2328 1879 1833 816 658 555 348 265 90 82 75 42 39 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 11035 11409 11555 387 Size: 1064 138 9446 374 146 1280 Fragments arranged by size: 9446 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3973 3973 Size: 12448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2241 2247 2422 2469 2738 2831 2861 Size: 393 609 6 175 47 269 93 30 Cuts at: 2861 2913 4014 5337 5572 6060 6878 7617 8081 Size: 52 1101 1323 235 488 818 739 464 Cuts at: 8081 8213 8260 8266 8622 8640 8912 8957 9035 Size: 132 47 6 356 18 272 45 78 Cuts at: 9035 9451 9712 10511 11108 11895 12163 1239 Size: 416 261 799 597 787 268 1524 Fragments arranged by size: 1524 1323 1101 818 799 787 739 609 597 488 464 416 393 356 272 269 268 261 235 175 132 93 78 52 47 47 45 30 18 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 5567 6767 6977 9302 5567 Size: 1200 210 2325 8713 Fragments arranged by size: 8713 2325 1200 210 EcoO109I rG'GnC_Cy Cuts at: 2494 2686 2801 2802 2897 3237 3766 7106 7858 Size: 192 115 1 95 340 529 3340 752 Cuts at: 7858 8050 9227 2494 Size: 192 1177 5715 Fragments arranged by size: 5715 3340 1177 752 529 340 192 192 115 95 1 EcoRI G'AATT_C Cuts at: 2182 4004 5336 6257 9757 10135 2182 Size: 1822 1332 921 3500 378 4495 Fragments arranged by size: 4495 3500 1822 1332 921 378 EcoRV GAT'ATC Cuts at: 6640 6640 Size: 12448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6088 6727 6088 Size: 639 11809 FspI TGC'GCA Cuts at: 5279 10954 5279 Size: 5675 6773 HaeII r_GCGC'y Cuts at: 1678 4105 7690 11595 1678 Size: 2427 3585 3905 2531 Fragments arranged by size: 3905 3585 2531 2427 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4072 4104 4728 4760 5455 Size: 32 135 32 2500 32 624 32 695 Cuts at: 5455 5487 5902 5934 5954 5986 6383 6415 6436 Size: 32 415 32 20 32 397 32 21 Cuts at: 6436 6468 7022 7054 8198 8230 8591 8623 8675 Size: 32 554 32 1144 32 361 32 52 Cuts at: 8675 8707 9638 9670 11089 11121 11163 11195 1373 Size: 32 931 32 1419 32 42 32 2626 Fragments arranged by size: 2626 2500 1419 1144 931 695 624 554 415 397 361 135 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 20 HincII GTy'rAC Cuts at: 669 1657 1938 7060 8793 9792 10038 669 Size: 988 281 5122 1733 999 246 3079 Fragments arranged by size: 5122 3079 1733 999 988 281 246 HindIII A'AGCT_T Cuts at: 748 5346 5435 6070 748 Size: 4598 89 635 7126 Fragments arranged by size: 7126 4598 635 89 HpaI GTT'AAC Cuts at: 8793 10038 8793 Size: 1245 11203 KpnI G_GTAC'C Cuts at: 9773 9773 Size: 12448 MfeI C'AATT_G Cuts at: 7005 10047 7005 Size: 3042 9406 MluI A'CGCG_T Cuts at: 12324 12324 Size: 12448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4431 5814 5914 6890 7008 9370 11562 11746 Size: 3126 1383 100 976 118 2362 2192 184 Cuts at: 11746 1305 Size: 2007 Fragments arranged by size: 3126 2362 2192 2007 1383 976 184 118 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 4685 5451 7559 8099 10 Size: 54 1425 343 2853 766 2108 540 4359 Fragments arranged by size: 4359 2853 2108 1425 766 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2913 4146 4664 5267 6324 6464 6486 Size: 648 1747 1233 518 603 1057 140 22 Cuts at: 6486 6927 8925 9393 10406 10765 10924 518 Size: 441 1998 468 1013 359 159 2042 Fragments arranged by size: 2042 1998 1747 1233 1057 1013 648 603 518 468 441 359 159 140 22 MspA1I CmG'CkG Cuts at: 1606 2429 2746 2965 3028 3429 5663 6536 7595 Size: 823 317 219 63 401 2234 873 1059 Cuts at: 7595 8708 9864 10541 11689 11934 1606 Size: 1113 1156 677 1148 245 2120 Fragments arranged by size: 2234 2120 1156 1148 1113 1059 873 823 677 401 317 245 219 63 NaeI GCC'GGC Cuts at: 5401 5401 Size: 12448 NcoI C'CATG_G Cuts at: 513 1065 2004 4579 4672 5385 7546 7822 8673 Size: 552 939 2575 93 713 2161 276 851 Cuts at: 8673 513 Size: 4288 Fragments arranged by size: 4288 2575 2161 939 851 713 552 276 93 NdeI CA'TA_TG Cuts at: 387 5938 6833 387 Size: 5551 895 6002 Fragments arranged by size: 6002 5551 895 NgoMIV G'CCGG_C Cuts at: 5399 5399 Size: 12448 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12448 NotI GC'GGCC_GC Cuts at: 9895 9895 Size: 12448 NspI r_CATG'y Cuts at: 1132 1229 2918 5248 9806 11351 1132 Size: 97 1689 2330 4558 1545 2229 Fragments arranged by size: 4558 2330 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 2690 2789 2845 1348 Size: 1342 99 56 10951 Fragments arranged by size: 10951 1342 99 56 PciI A'CATG_T Cuts at: 11347 11347 Size: 12448 PflMI CCAn_nnn'nTGG Cuts at: 1108 2891 3221 6123 7595 7773 8557 1108 Size: 1783 330 2902 1472 178 784 4999 Fragments arranged by size: 4999 2902 1783 1472 784 330 178 PfoI T'CCnGG_A Cuts at: 8168 8617 8168 Size: 449 11999 PmeI GTTT'AAAC Cuts at: 9752 9752 Size: 12448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10517 10549 12030 12062 10517 Size: 32 1481 32 10903 Fragments arranged by size: 10903 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2897 3766 7106 7858 8050 2686 Size: 211 869 3340 752 192 7084 Fragments arranged by size: 7084 3340 869 752 211 192 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12448 PsiI TTA'TAA Cuts at: 6702 10018 6702 Size: 3316 9132 PspOMI G'GGCC_C Cuts at: 2801 9258 2801 Size: 6457 5991 PspXI vC'TCGA_Gb Cuts at: 3447 3447 Size: 12448 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3249 3281 3249 Size: 32 12416 PstI C_TGCA'G Cuts at: 830 2821 3033 3213 5382 5718 7315 8442 8698 Size: 1991 212 180 2169 336 1597 1127 256 Cuts at: 8698 9800 830 Size: 1102 3478 Fragments arranged by size: 3478 2169 1991 1597 1127 1102 336 256 212 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12448 PvuII CAG'CTG Cuts at: 2429 2746 2965 3028 5663 6536 7595 2429 Size: 317 219 63 2635 873 1059 7282 Fragments arranged by size: 7282 2635 1059 873 317 219 63 SacI G_AGCT'C Cuts at: 3975 3975 Size: 12448 SacII CC_GC'GG Cuts at: 3430 3430 Size: 12448 SalI G'TCGA_C Cuts at: 1936 9790 1936 Size: 7854 4594 SapI GCTCTTCn'nnn_ Cuts at: 3232 4146 8904 3232 Size: 914 4758 6776 Fragments arranged by size: 6776 4758 914 SbfI CC_TGCA'GG Cuts at: 5382 5718 9800 5382 Size: 336 4082 8030 Fragments arranged by size: 8030 4082 336 ScaI AGT'ACT Cuts at: 7541 10696 12408 7541 Size: 3155 1712 7581 Fragments arranged by size: 7581 3155 1712 SexAI A'CCwGG_T Cuts at: 2082 2641 4245 6249 2082 Size: 559 1604 2004 8281 Fragments arranged by size: 8281 2004 1604 559 SfcI C'TryA_G Cuts at: 826 1045 2817 3029 3209 3255 3618 4953 5066 Size: 219 1772 212 180 46 363 1335 113 Cuts at: 5066 5378 5714 7311 8438 8694 9365 9796 10931 Size: 312 336 1597 1127 256 671 431 1135 Cuts at: 10931 11612 11803 826 Size: 681 191 1471 Fragments arranged by size: 1772 1597 1471 1335 1135 1127 681 671 431 363 336 312 256 219 212 191 180 113 46 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12448 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12448 SmaI CCC'GGG Cuts at: 1872 5633 9775 1872 Size: 3761 4142 4545 Fragments arranged by size: 4545 4142 3761 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 2979 3447 3693 4895 5021 Size: 197 926 546 490 468 246 1202 126 Cuts at: 5021 5039 5078 5822 8227 9535 10552 11453 11715 Size: 18 39 744 2405 1308 1017 901 262 Cuts at: 11715 820 Size: 1553 Fragments arranged by size: 2405 1553 1308 1202 1017 926 901 744 546 490 468 262 246 197 126 39 18 SnaBI TAC'GTA Cuts at: 493 4174 10239 493 Size: 3681 6065 2702 Fragments arranged by size: 6065 3681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12448 SphI G_CATG'C Cuts at: 1132 9806 1132 Size: 8674 3774 SspI AAT'ATT Cuts at: 5 52 6502 10372 5 Size: 47 6450 3870 2081 Fragments arranged by size: 6450 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 12448 StyI C'CwwG_G Cuts at: 513 1065 2004 2113 2139 2395 2545 2682 2902 Size: 552 939 109 26 256 150 137 220 Cuts at: 2902 2995 3009 3232 4579 4672 5385 7102 7546 Size: 93 14 223 1347 93 713 1717 444 Cuts at: 7546 7822 8469 8673 9887 513 Size: 276 647 204 1214 3074 Fragments arranged by size: 3074 1717 1347 1214 939 713 647 552 444 276 256 223 220 204 150 137 109 93 93 26 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2402 3128 4365 5146 5310 8975 9425 Size: 1255 822 726 1237 781 164 3665 450 Cuts at: 9425 10475 10492 10645 10830 11275 325 Size: 1050 17 153 185 445 1498 Fragments arranged by size: 3665 1498 1255 1237 1050 822 781 726 450 445 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3136 4017 4716 Size: 274 80 33 51 1443 1159 881 699 Cuts at: 4716 6468 7539 8515 9154 10694 12406 96 Size: 1752 1071 976 639 1540 1712 138 Fragments arranged by size: 1752 1712 1540 1443 1159 1071 976 881 699 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3505 3537 3598 3630 7390 7422 8885 8917 12408 Size: 32 61 32 3760 32 1463 32 3491 Cuts at: 12408 12440 3505 Size: 32 3513 Fragments arranged by size: 3760 3513 3491 1463 61 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3127 8501 1316 Size: 1811 5374 5263 Fragments arranged by size: 5374 5263 1811 XbaI T'CTAG_A Cuts at: 9170 9784 9170 Size: 614 11834 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5824 6873 5824 Size: 1049 11399 XhoI C'TCGA_G Cuts at: 1943 3447 3693 1943 Size: 1504 246 10698 Fragments arranged by size: 10698 1504 246 XmaI C'CCGG_G Cuts at: 1870 5631 9773 1870 Size: 3761 4142 4545 Fragments arranged by size: 4545 4142 3761 XmnI GAAnn'nnTTC Cuts at: 2405 5055 5207 6049 7680 10577 2405 Size: 2650 152 842 1631 2897 4276 Fragments arranged by size: 4276 2897 2650 1631 842 152 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10256 276 Size: 53 83 186 936 8722 2468 Fragments arranged by size: 8722 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI FseI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SanDI SfiI SfoI SrfI SwaI