Restriction map for
pFN21AE9652
(Linear) (Six-base) MAPSORT of: KIEE9652.seq Check: 7830 from: 1 to: 7743
KIEE9652.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1017 3380 7743
Size: 1017 2363 4363
Fragments arranged by size:
4363 2363 1017
AccI GT'mk_AC
Cuts at: 0 927 1931 5431 5965 7457 7743
Size: 927 1004 3500 534 1492 286
Fragments arranged by size:
3500 1492 1004 927 534 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 2009 3332 3567 4055 7743
Size: 826 1183 1323 235 488 3688
Fragments arranged by size:
3688 1323 1183 826 488 235
AfeI AGC'GCT
Cuts at: 0 2098 7743
Size: 2098 5645
AflII C'TTAA_G
Cuts at: 0 484 3073 7743
Size: 484 2589 4670
Fragments arranged by size:
4670 2589 484
AflIII A'CryG_T
Cuts at: 0 7334 7743
Size: 7334 409
AhdI GACnn_n'nnGTC
Cuts at: 0 4445 7743
Size: 4445 3298
AleI CACnn'nnGTG
Cuts at: 0 2659 4481 7388 7743
Size: 2659 1822 2907 355
Fragments arranged by size:
2907 2659 1822 355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2396 5863 6604 7743
Size: 2396 3467 741 1139
Fragments arranged by size:
3467 2396 1139 741
AlwNI CAG_nnn'CTG
Cuts at: 0 511 567 772 3661 5590 7743
Size: 511 56 205 2889 1929 2153
Fragments arranged by size:
2889 2153 1929 511 205 56
ApaI G_GGCC'C
Cuts at: 0 800 7257 7743
Size: 800 6457 486
Fragments arranged by size:
6457 800 486
ApaLI G'TGCA_C
Cuts at: 0 7436 7743
Size: 7436 307
ApoI r'AATT_y
Cuts at: 0 36 177 530 1999 2320 2629 3201 3331
Size: 36 141 353 1469 321 309 572 130
Cuts at: 3331 4252 6566 7743
Size: 921 2314 1177
Fragments arranged by size:
2314 1469 1177 921 572 353 321 309
141 130 36
AseI AT'TA_AT
Cuts at: 0 3647 7743
Size: 3647 4096
AvaI C'yCGr_G
Cuts at: 0 1442 1688 3626 6025 7743
Size: 1442 246 1938 2399 1718
Fragments arranged by size:
2399 1938 1718 1442 246
BamHI G'GATC_C
Cuts at: 0 4652 6941 7743
Size: 4652 2289 802
Fragments arranged by size:
4652 2289 802
BanI G'GyrC_C
Cuts at: 0 514 1567 2342 3123 7743
Size: 514 1053 775 781 4620
Fragments arranged by size:
4620 1053 781 775 514
BanII G_rGCy'C
Cuts at: 0 800 1247 1416 1970 2153 2228 5227 5630
Size: 800 447 169 554 183 75 2999 403
Cuts at: 5630 7257 7539 7743
Size: 1627 282 204
Fragments arranged by size:
2999 1627 800 554 447 403 282 204
183 169 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 3099 3603 4462 7743
Size: 3099 504 859 3281
Fragments arranged by size:
3281 3099 859 504
BciVI GTATCCnnnnn_n'
Cuts at: 0 2072 6144 7743
Size: 2072 4072 1599
Fragments arranged by size:
4072 2072 1599
BclI T'GATC_A
Cuts at: 0 2768 7743
Size: 2768 4975
BglI GCCn_nnn'nGGC
Cuts at: 0 523 4138 5781 7743
Size: 523 3615 1643 1962
Fragments arranged by size:
3615 1962 1643 523
BglII A'GATC_T
Cuts at: 0 3912 3995 5156 7743
Size: 3912 83 1161 2587
Fragments arranged by size:
3912 2587 1161 83
BlpI GC'TnA_GC
Cuts at: 0 560 7743
Size: 560 7183
Bme1580I G_kGCm'C
Cuts at: 0 519 800 1422 7257 7440 7743
Size: 519 281 622 5835 183 303
Fragments arranged by size:
5835 622 519 303 281 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1901 2628 4831 6044 6705 7322 7743
Size: 1901 727 2203 1213 661 617 421
Fragments arranged by size:
2203 1901 1213 727 661 617 421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5175 5664 7743
Size: 5175 489 2079
Fragments arranged by size:
5175 2079 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 242 417 464 733 856 908 4873
Size: 236 6 175 47 269 123 52 3965
Cuts at: 4873 5612 6076 6208 6255 6261 6617 6635 6907
Size: 739 464 132 47 6 356 18 272
Cuts at: 6907 6952 7030 7446 7707 7743
Size: 45 78 416 261 36
Fragments arranged by size:
3965 739 464 416 356 272 269 261
236 175 132 123 78 52 47 47
45 36 18 6 6
Bpu10I CC'TnA_GC
Cuts at: 0 385 1829 2804 4058 6419 7743
Size: 385 1444 975 1254 2361 1324
Fragments arranged by size:
2361 1444 1324 1254 975 385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 2875 3001 3019 3802 6243 7551 7743
Size: 959 1916 126 18 783 2441 1308 192
Fragments arranged by size:
2441 1916 1308 959 783 192 126 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 4432 4772 6928 7322 7743
Size: 4432 340 2156 394 421
Fragments arranged by size:
4432 2156 421 394 340
BsaAI yAC'GTr
Cuts at: 0 2169 7743
Size: 2169 5574
BsaBI GATnn'nnATC
Cuts at: 0 478 5417 6836 7743
Size: 478 4939 1419 907
Fragments arranged by size:
4939 1419 907 478
BsaHI Gr'CG_yC
Cuts at: 0 340 2733 5250 7743
Size: 340 2393 2517 2493
Fragments arranged by size:
2517 2493 2393 340
BsaWI w'CCGG_w
Cuts at: 0 4655 7743
Size: 4655 3088
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1593 1623 6880 6910 7003 7033 7705 7735
Size: 1593 30 5257 30 93 30 672 30
Cuts at: 7735 7743
Size: 8
Fragments arranged by size:
5257 1593 672 93 30 30 30 30
8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 420 492 1301 1498 1552 3528 5167 5653
Size: 420 72 809 197 54 1976 1639 486
Cuts at: 5653 6043 6691 7624 7743
Size: 390 648 933 119
Fragments arranged by size:
1976 1639 933 809 648 486 420 390
197 119 72 54
BseYI C'CCAG_C
Cuts at: 0 721 1091 1109 1468 1519 4527 4953 5583
Size: 721 370 18 359 51 3008 426 630
Cuts at: 5583 7743
Size: 2160
Fragments arranged by size:
3008 2160 721 630 426 370 359 51
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1661 5938 6680 7743
Size: 1051 610 4277 742 1063
Fragments arranged by size:
4277 1063 1051 742 610
BsiHKAI G_wGCw'C
Cuts at: 0 1970 2257 5916 6083 7440 7743
Size: 1970 287 3659 167 1357 303
Fragments arranged by size:
3659 1970 1357 303 287 167
BsmI GAATG_Cn'
Cuts at: 0 990 1709 4751 7743
Size: 990 719 3042 2992
Fragments arranged by size:
3042 2992 990 719
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1713 7743
Size: 1713 6030
Bsp1286I G_dGCh'C
Cuts at: 0 519 800 1247 1416 1422 1970 2153 2228
Size: 519 281 447 169 6 548 183 75
Cuts at: 2228 2257 5227 5630 5916 6083 7257 7440 7539
Size: 29 2970 403 286 167 1174 183 99
Cuts at: 7539 7743
Size: 204
Fragments arranged by size:
2970 1174 548 519 447 403 286 281
204 183 183 169 167 99 75 29
6
BspEI T'CCGG_A
Cuts at: 0 4655 7743
Size: 4655 3088
BspHI T'CATG_A
Cuts at: 0 3360 7743
Size: 3360 4383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1017 3217 3380 3676 3716 7743
Size: 1017 2200 163 296 40 4027
Fragments arranged by size:
4027 2200 1017 296 163 40
BsrBI CCG'CTC
Cuts at: 0 4284 6332 7743
Size: 4284 2048 1411
Fragments arranged by size:
4284 2048 1411
BsrDI GCAATG_nn'
Cuts at: 0 1403 2537 2591 2935 7743
Size: 1403 1134 54 344 4808
Fragments arranged by size:
4808 1403 1134 344 54
BsrFI r'CCGG_y
Cuts at: 0 619 3394 5321 7743
Size: 619 2775 1927 2422
Fragments arranged by size:
2775 2422 1927 619
BsrGI T'GTAC_A
Cuts at: 0 1131 7743
Size: 1131 6612
BssSI C'ACGA_G
Cuts at: 0 4459 4589 6082 7743
Size: 4459 130 1493 1661
Fragments arranged by size:
4459 1661 1493 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 915 1306 3281 4334 7743
Size: 915 391 1975 1053 3409
Fragments arranged by size:
3409 1975 1053 915 391
BstEII G'GTnAC_C
Cuts at: 0 5187 7743
Size: 5187 2556
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 1278 4868 7743
Size: 157 1121 3590 2875
Fragments arranged by size:
3590 2875 1121 157
BstYI r'GATC_y
Cuts at: 0 18 380 3030 3912 3995 4155 4652 5076
Size: 18 362 2650 882 83 160 497 424
Cuts at: 5076 5156 6941 7743
Size: 80 1785 802
Fragments arranged by size:
2650 1785 882 802 497 424 362 160
83 80 18
BstZ17I GTA'TAC
Cuts at: 0 5966 7743
Size: 5966 1777
Bsu36I CC'TnA_GG
Cuts at: 0 4759 7607 7743
Size: 4759 2848 136
Fragments arranged by size:
4759 2848 136
BtgI C'CryG_G
Cuts at: 0 1422 2574 2667 3380 4482 5541 5817 6668
Size: 1422 1152 93 713 1102 1059 276 851
Cuts at: 6668 7743
Size: 1075
Fragments arranged by size:
1422 1152 1102 1075 1059 851 713 276
93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1061 7743
Size: 1061 6682
BtsI GCAGTG_nn'
Cuts at: 0 5947 5975 5986 7395 7597 7743
Size: 5947 28 11 1409 202 146
Fragments arranged by size:
5947 1409 202 146 28 11
DraI TTT'AAA
Cuts at: 0 2325 4914 6071 7743
Size: 2325 2589 1157 1672
Fragments arranged by size:
2589 2325 1672 1157
DraIII CAC_nnn'GTG
Cuts at: 0 2916 6087 7743
Size: 2916 3171 1656
Fragments arranged by size:
3171 2916 1656
DrdI GACnn_nn'nnGTC
Cuts at: 0 3607 4307 6634 7743
Size: 3607 700 2327 1109
Fragments arranged by size:
3607 2327 1109 700
EaeI y'GGCC_r
Cuts at: 0 671 1477 2678 3444 4285 4980 5552 6092
Size: 671 806 1201 766 841 695 572 540
Cuts at: 6092 7743
Size: 1651
Fragments arranged by size:
1651 1201 841 806 766 695 671 572
540
EarI CTCTTCn'nnn_
Cuts at: 0 1227 1242 1507 1546 1621 1711 1793 2141
Size: 1227 15 265 39 75 90 82 348
Cuts at: 2141 3974 6302 6344 6899 7557 7743
Size: 1833 2328 42 555 658 186
Fragments arranged by size:
2328 1833 1227 658 555 348 265 186
90 82 75 42 39 15
EcoICRI GAG'CTC
Cuts at: 0 1968 7743
Size: 1968 5775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 242 417 464 733 826 856 908
Size: 236 6 175 47 269 93 30 52
Cuts at: 908 2009 3332 3567 4055 4873 5612 6076 6208
Size: 1101 1323 235 488 818 739 464 132
Cuts at: 6208 6255 6261 6617 6635 6907 6952 7030 7446
Size: 47 6 356 18 272 45 78 416
Cuts at: 7446 7707 7743
Size: 261 36
Fragments arranged by size:
1323 1101 818 739 488 464 416 356
272 269 261 236 235 175 132 93
78 52 47 47 45 36 30 18
6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3562 4762 4972 7297 7743
Size: 3562 1200 210 2325 446
Fragments arranged by size:
3562 2325 1200 446 210
EcoO109I rG'GnC_Cy
Cuts at: 0 489 681 796 797 892 1232 1761 5101
Size: 489 192 115 1 95 340 529 3340
Cuts at: 5101 5853 6045 7222 7743
Size: 752 192 1177 521
Fragments arranged by size:
3340 1177 752 529 521 489 340 192
192 115 95 1
EcoRI G'AATT_C
Cuts at: 0 177 1999 3331 4252 7743
Size: 177 1822 1332 921 3491
Fragments arranged by size:
3491 1822 1332 921 177
EcoRV GAT'ATC
Cuts at: 0 4635 7743
Size: 4635 3108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4083 4722 7743
Size: 4083 639 3021
Fragments arranged by size:
4083 3021 639
FspI TGC'GCA
Cuts at: 0 3274 7743
Size: 3274 4469
HaeII r_GCGC'y
Cuts at: 0 2100 5685 7743
Size: 2100 3585 2058
Fragments arranged by size:
3585 2100 2058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2067 2099 2723 2755 3450 3482 3897 3929
Size: 2067 32 624 32 695 32 415 32
Cuts at: 3929 3949 3981 4378 4410 4431 4463 5017 5049
Size: 20 32 397 32 21 32 554 32
Cuts at: 5049 6193 6225 6586 6618 6670 6702 7633 7665
Size: 1144 32 361 32 52 32 931 32
Cuts at: 7665 7743
Size: 78
Fragments arranged by size:
2067 1144 931 695 624 554 415 397
361 78 52 32 32 32 32 32
32 32 32 32 32 32 32 21
20
HincII GTy'rAC
Cuts at: 0 5055 6788 7743
Size: 5055 1733 955
Fragments arranged by size:
5055 1733 955
HindIII A'AGCT_T
Cuts at: 0 3341 3430 4065 7743
Size: 3341 89 635 3678
Fragments arranged by size:
3678 3341 635 89
HpaI GTT'AAC
Cuts at: 0 6788 7743
Size: 6788 955
MfeI C'AATT_G
Cuts at: 0 5000 7743
Size: 5000 2743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2426 3809 3909 4885 5003 7365 7743
Size: 2426 1383 100 976 118 2362 378
Fragments arranged by size:
2426 2362 1383 976 378 118 100
MscI TGG'CCA
Cuts at: 0 2680 3446 5554 6094 7743
Size: 2680 766 2108 540 1649
Fragments arranged by size:
2680 2108 1649 766 540
MslI CAynn'nnrTG
Cuts at: 0 908 2141 2659 3262 4319 4459 4481 4922
Size: 908 1233 518 603 1057 140 22 441
Cuts at: 4922 6920 7388 7743
Size: 1998 468 355
Fragments arranged by size:
1998 1233 1057 908 603 518 468 441
355 140 22
MspA1I CmG'CkG
Cuts at: 0 424 741 960 1023 1424 3658 4531 5590
Size: 424 317 219 63 401 2234 873 1059
Cuts at: 5590 6703 7743
Size: 1113 1040
Fragments arranged by size:
2234 1113 1059 1040 873 424 401 317
219 63
NaeI GCC'GGC
Cuts at: 0 3396 7743
Size: 3396 4347
NcoI C'CATG_G
Cuts at: 0 2574 2667 3380 5541 5817 6668 7743
Size: 2574 93 713 2161 276 851 1075
Fragments arranged by size:
2574 2161 1075 851 713 276 93
NdeI CA'TA_TG
Cuts at: 0 3933 4828 7743
Size: 3933 895 2915
Fragments arranged by size:
3933 2915 895
NgoMIV G'CCGG_C
Cuts at: 0 3394 7743
Size: 3394 4349
NspI r_CATG'y
Cuts at: 0 913 3243 7743
Size: 913 2330 4500
Fragments arranged by size:
4500 2330 913
PasI CC'CwG_GG
Cuts at: 0 685 784 840 7743
Size: 685 99 56 6903
Fragments arranged by size:
6903 685 99 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 886 1216 4118 5590 5768 6552 7743
Size: 886 330 2902 1472 178 784 1191
Fragments arranged by size:
2902 1472 1191 886 784 330 178
PfoI T'CCnGG_A
Cuts at: 0 6163 6612 7743
Size: 6163 449 1131
Fragments arranged by size:
6163 1131 449
PpuMI rG'GwC_Cy
Cuts at: 0 681 892 1761 5101 5853 6045 7743
Size: 681 211 869 3340 752 192 1698
Fragments arranged by size:
3340 1698 869 752 681 211 192
PsiI TTA'TAA
Cuts at: 0 4697 7743
Size: 4697 3046
PspOMI G'GGCC_C
Cuts at: 0 796 7253 7743
Size: 796 6457 490
Fragments arranged by size:
6457 796 490
PspXI vC'TCGA_Gb
Cuts at: 0 1442 7743
Size: 1442 6301
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1244 1276 7743
Size: 1244 32 6467
Fragments arranged by size:
6467 1244 32
PstI C_TGCA'G
Cuts at: 0 816 1028 1208 3377 3713 5310 6437 6693
Size: 816 212 180 2169 336 1597 1127 256
Cuts at: 6693 7743
Size: 1050
Fragments arranged by size:
2169 1597 1127 1050 816 336 256 212
180
PvuII CAG'CTG
Cuts at: 0 424 741 960 1023 3658 4531 5590 7743
Size: 424 317 219 63 2635 873 1059 2153
Fragments arranged by size:
2635 2153 1059 873 424 317 219 63
SacI G_AGCT'C
Cuts at: 0 1970 7743
Size: 1970 5773
SacII CC_GC'GG
Cuts at: 0 1425 7743
Size: 1425 6318
SapI GCTCTTCn'nnn_
Cuts at: 0 1227 2141 6899 7743
Size: 1227 914 4758 844
Fragments arranged by size:
4758 1227 914 844
SbfI CC_TGCA'GG
Cuts at: 0 3377 3713 7743
Size: 3377 336 4030
Fragments arranged by size:
4030 3377 336
ScaI AGT'ACT
Cuts at: 0 5536 7743
Size: 5536 2207
SexAI A'CCwGG_T
Cuts at: 0 77 636 2240 4244 7743
Size: 77 559 1604 2004 3499
Fragments arranged by size:
3499 2004 1604 559 77
SfcI C'TryA_G
Cuts at: 0 812 1024 1204 1250 1613 2948 3061 3373
Size: 812 212 180 46 363 1335 113 312
Cuts at: 3373 3709 5306 6433 6689 7360 7743
Size: 336 1597 1127 256 671 383
Fragments arranged by size:
1597 1335 1127 812 671 383 363 336
312 256 212 180 113 46
SmaI CCC'GGG
Cuts at: 0 3628 7743
Size: 3628 4115
SmlI C'TyrA_G
Cuts at: 0 484 974 1442 1688 2890 3016 3034 3073
Size: 484 490 468 246 1202 126 18 39
Cuts at: 3073 3817 6222 7530 7743
Size: 744 2405 1308 213
Fragments arranged by size:
2405 1308 1202 744 490 484 468 246
213 126 39 18
SnaBI TAC'GTA
Cuts at: 0 2169 7743
Size: 2169 5574
SspI AAT'ATT
Cuts at: 0 4497 7743
Size: 4497 3246
StyI C'CwwG_G
Cuts at: 0 108 134 390 540 677 897 990 1004
Size: 108 26 256 150 137 220 93 14
Cuts at: 1004 1227 2574 2667 3380 5097 5541 5817 6464
Size: 223 1347 93 713 1717 444 276 647
Cuts at: 6464 6668 7743
Size: 204 1075
Fragments arranged by size:
1717 1347 1075 713 647 444 276 256
223 220 204 150 137 108 93 93
26 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 1123 2360 3141 3305 6970 7420 7743
Size: 397 726 1237 781 164 3665 450 323
Fragments arranged by size:
3665 1237 781 726 450 397 323 164
TatI w'GTAC_w
Cuts at: 0 1131 2012 2711 4463 5534 6510 7149 7743
Size: 1131 881 699 1752 1071 976 639 594
Fragments arranged by size:
1752 1131 1071 976 881 699 639 594
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1500 1532 1593 1625 5385 5417 6880 6912
Size: 1500 32 61 32 3760 32 1463 32
Cuts at: 6912 7743
Size: 831
Fragments arranged by size:
3760 1500 1463 831 61 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1122 6496 7743
Size: 1122 5374 1247
Fragments arranged by size:
5374 1247 1122
XbaI T'CTAG_A
Cuts at: 0 7165 7743
Size: 7165 578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3819 4868 7743
Size: 3819 1049 2875
Fragments arranged by size:
3819 2875 1049
XhoI C'TCGA_G
Cuts at: 0 1442 1688 7743
Size: 1442 246 6055
Fragments arranged by size:
6055 1442 246
XmaI C'CCGG_G
Cuts at: 0 3626 7743
Size: 3626 4117
XmnI GAAnn'nnTTC
Cuts at: 0 400 3050 3202 4044 5675 7743
Size: 400 2650 152 842 1631 2068
Fragments arranged by size:
2650 2068 1631 842 400 152
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PasI PflMI PfoI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuII SacI SacII SapI SbfI ScaI SexAI
SfcI SmaI SmlI SnaBI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AloI AscI AsiSI AvrII
BaeI BbeI BbvCI BcgI BfrBI BmgBI BmtI BsiEI
BsiWI BssHII BstBI ClaI CspCI EagI EciI FseI
FspAI KasI KpnI MluI NarI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PvuI
RsrII SalI SanDI SfiI SfoI SgfI SgrAI SpeI
SphI SrfI StuI SwaI ZraI