################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AviII AvrII BalI BbeI BbrPI BcgI BclI BglI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DsaI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AatII 1672 GACGTC GACGT^C AccI 2357 GTCGAC GT^[AC][GT]AC AccII 1639 CGCG CG^CG AccIII 112 TCCGGA T^CCGGA AcsI 1459 GAATTT [GA]^AATT[CT] AcyI 1672 GACGTC G[GA]^CG[CT]C AfaI 55 GTAC GT^AC AflII 1792 CTTAAG C^TTAAG AocI 655 CCTCAGG CC^T[ACGT]AGG ApoI 1459 GAATTT [GA]^AATT[CT] AseI 2040 ATTAAT AT^TAAT AsnI 2040 ATTAAT AT^TAAT Asp700I 796 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 487 GAGCTC G[AT]GC[AT]^C AspI 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 890 CCCGAG C^[CT]CG[GA]G BamHI 837 GGATCC G^GATCC BbvCI 1711 GCTGAGG CCTCAGC(-5/-2) BciVI 1020 GGATAC GTATCC(6/5) BcnI 1899 CCGGG CC^[GC]GG BfrI 1792 CTTAAG C^TTAAG BsaBI 334 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1672 GACGTC G[GA]^CG[CT]C BsaWI 112 TCCGGA [AT]^CCGG[AT] BseAI 112 TCCGGA T^CCGGA BsiHKAI 487 GAGCTC G[AT]GC[AT]^C BsmFI 2260 GTCCC GGGAC(10/14) BsoBI 890 CCCGAG C^[CT]CG[GA]G BspEI 112 TCCGGA T^CCGGA BsrDI 1397 GCAATG GCAATG(2/0) BstAPI 1923 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1639 CGCG CG^CG Bsu36I 655 CCTCAGG CC^T[ACGT]AGG CfoI 1626 GCGC GCG^C DraIII 2416 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2281 GACTATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 655 CCTCAGG CC^T[ACGT]AGG EcoNI 2187 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 463 CCCGC CCCGC(4/6) HgaI 958 GCGTC GACGC(5/10) HhaI 1626 GCGC GCG^C Hin1I 1672 GACGTC G[GA]^CG[CT]C HinP1I 1626 GCGC G^CGC HincII 2357 GTCGAC GT[CT]^[GA]AC HindII 2357 GTCGAC GT[CT]^[GA]AC MamI 334 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 112 TCCGGA T^CCGGA MslI 424 CATGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 18 CAGCGG C[AC]G^C[GT]G MvnI 1639 CGCG CG^CG NciI 1899 CCGGG CC^[GC]GG NspI 423 GCATGC [GA]CATG^[CT] PflFI 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2040 ATTAAT AT^TAAT RsaI 55 GTAC GT^AC SacI 487 GAGCTC GAGCT^C SalI 2357 GTCGAC G^TCGAC SapI 796 GAAGAGC GCTCTTC(1/4) SexAI 384 ACCTGGT A^CC[AT]GGT SfcI 1778 CTGTAG C^T[GA][CT]AG SpeI 157 ACTAGT A^CTAGT SphI 423 GCATGC GCATG^C SspI 11 AATATT AAT^ATT StuI 2059 AGGCCT AGG^CCT Tth111I 1669 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2124 TCTAGA T^CTAGA XmnI 796 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AhdI 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1570 GACTTTCTGTC AspEI 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1570 GACTTTCTGTC BanII 217 GAGCCC G[GA]GC[CT]^C 487 GAGCTC BbsI 1299 GAAGAC GAAGAC(2/6) 1717 GAAGAC BpmI 490 CTCCAG CTGGAG(16/14) 2228 CTGGAG BpuAI 1299 GAAGAC GAAGAC(2/6) 1717 GAAGAC Eam1105I 1384 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1570 GACTTTCTGTC HapII 113 CCGG C^CGG 1899 CCGG HpaII 113 CCGG C^CGG 1899 CCGG MspI 113 CCGG C^CGG 1899 CCGG NdeI 1060 CATATG CA^TATG 1564 CATATG SmlI 1792 CTTAAG C^T[CT][GA]AG 1891 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanI BbvI BfaI BglII BmyI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII