(Circular) (Six-base) MAPSORT of: pFN21AE3310.seq Check: 1606 from: 1 to: 7162 pFN21AE3310 7162 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3681 4972 278 Size: 53 83 186 936 2145 1291 2468 Fragments arranged by size: 2468 2145 1291 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4363 4505 1937 Size: 2426 142 4594 Fragments arranged by size: 4594 2426 142 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 5289 5662 1563 Size: 3726 373 3063 Fragments arranged by size: 3726 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3938 3980 5225 6609 6877 1632 Size: 2306 42 1245 1384 268 1917 Fragments arranged by size: 2306 1917 1384 1245 268 42 AflII C'TTAA_G Cuts at: 820 1017 3797 820 Size: 197 2780 4185 Fragments arranged by size: 4185 2780 197 AflIII A'CryG_T Cuts at: 1203 6061 7038 1203 Size: 4858 977 1327 Fragments arranged by size: 4858 1327 977 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 3394 3580 5891 3394 Size: 186 2311 4665 Fragments arranged by size: 4665 2311 186 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4469 1184 Size: 3285 3877 AlwNI CAG_nnn'CTG Cuts at: 2129 2210 2954 3542 4905 6477 2129 Size: 81 744 588 1363 1572 2814 Fragments arranged by size: 2814 1572 1363 744 588 81 ApaLI G'TGCA_C Cuts at: 5219 6375 5219 Size: 1156 6006 ApoI r'AATT_y Cuts at: 1493 3464 4471 4697 4849 6958 1493 Size: 1971 1007 226 152 2109 1697 Fragments arranged by size: 2109 1971 1697 1007 226 152 AseI AT'TA_AT Cuts at: 160 1032 4046 5716 160 Size: 872 3014 1670 1606 Fragments arranged by size: 3014 1670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 2895 4487 1870 Size: 73 952 1592 4545 Fragments arranged by size: 4545 1592 952 73 BamHI G'GATC_C Cuts at: 2842 4492 4859 2842 Size: 1650 367 5145 Fragments arranged by size: 5145 1650 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2580 2697 2775 3653 4483 Size: 325 217 627 793 117 78 878 830 Cuts at: 4483 5938 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 878 830 793 627 325 217 117 78 BanII G_rGCy'C Cuts at: 1391 2226 2496 4481 1391 Size: 835 270 1985 4072 Fragments arranged by size: 4072 1985 835 270 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3311 3729 928 Size: 971 1412 418 4361 Fragments arranged by size: 4361 1412 971 418 BbvCI CC'TCA_GC Cuts at: 3717 3717 Size: 7162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5353 5387 1903 Size: 34 3416 34 3678 Fragments arranged by size: 3678 3416 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3019 5054 6264 3019 Size: 2035 1210 3917 Fragments arranged by size: 3917 2035 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5773 136 Size: 107 122 71 631 605 4101 1525 Fragments arranged by size: 4101 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3487 4126 4412 7157 3487 Size: 639 286 2745 3492 Fragments arranged by size: 3492 2745 639 286 BlpI GC'TnA_GC Cuts at: 4579 4579 Size: 7162 Bme1580I G_kGCm'C Cuts at: 1790 4255 5223 6379 1790 Size: 2465 968 1156 2573 Fragments arranged by size: 2573 2465 1156 968 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7162 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2051 4893 5851 Size: 416 53 83 399 24 634 2842 958 Cuts at: 5851 442 Size: 1753 Fragments arranged by size: 2842 1753 958 634 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 7162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 4254 5822 1239 Size: 1241 1774 1568 2579 Fragments arranged by size: 2579 1774 1568 1241 Bpu10I CC'TnA_GC Cuts at: 3717 3717 Size: 7162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3881 5287 6152 6450 3881 Size: 1406 865 298 4593 Fragments arranged by size: 4593 1406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2369 3947 4182 5825 882 Size: 617 870 1578 235 1643 2219 Fragments arranged by size: 2219 1643 1578 870 617 235 BsaAI yAC'GTr Cuts at: 493 1204 4953 493 Size: 711 3749 2702 Fragments arranged by size: 3749 2702 711 BsaBI GATnn'nnATC Cuts at: 2343 7156 2343 Size: 4813 2349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3678 4969 5351 5986 Size: 53 83 186 936 2145 1291 382 635 Cuts at: 5986 275 Size: 1451 Fragments arranged by size: 2145 1451 1291 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2117 5595 5993 6267 6414 6825 2117 Size: 3478 398 274 147 411 2454 Fragments arranged by size: 3478 2454 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3376 3406 4004 4034 1178 Size: 30 165 30 1973 30 598 30 4306 Fragments arranged by size: 4306 1973 598 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2504 2621 2660 2738 2894 3116 3173 Size: 1087 231 117 39 78 156 222 57 Cuts at: 3173 3176 3923 4229 1186 Size: 3 747 306 4119 Fragments arranged by size: 4119 1087 747 306 231 222 156 117 78 57 39 3 BseYI C'CCAG_C Cuts at: 3335 6365 6861 3335 Size: 3030 496 3636 Fragments arranged by size: 3636 3030 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2325 3876 4401 1163 Size: 573 589 1551 525 3924 Fragments arranged by size: 3924 1551 589 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4612 5373 6401 1540 Size: 275 186 2611 761 1028 2301 Fragments arranged by size: 2611 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 5223 5308 6379 2496 Size: 2727 85 1071 3279 Fragments arranged by size: 3279 2727 1071 85 BsmI GAATG_Cn' Cuts at: 4673 4766 4673 Size: 93 7069 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2496 4255 4481 5223 5308 6379 Size: 399 436 270 1759 226 742 85 1071 Cuts at: 6379 1391 Size: 2174 Fragments arranged by size: 2174 1759 1071 742 436 399 270 226 85 BspEI T'CCGG_A Cuts at: 2117 6825 2117 Size: 4708 2454 BspHI T'CATG_A Cuts at: 5051 6781 5051 Size: 1730 5432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4517 844 Size: 3673 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5049 1394 Size: 429 3226 3507 Fragments arranged by size: 3507 3226 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3409 5657 5831 65 Size: 1183 2161 2248 174 1396 Fragments arranged by size: 2248 2161 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5806 5993 1700 Size: 110 3996 187 2869 Fragments arranged by size: 3996 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7162 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 5222 6234 5222 Size: 1012 6150 BstAPI GCAn_nnn'nTGC Cuts at: 794 3934 794 Size: 3140 4022 BstBI TT'CG_AA Cuts at: 5966 5966 Size: 7162 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7162 BstYI r'GATC_y Cuts at: 1972 2842 3373 3487 3900 3921 4126 4237 4412 Size: 870 531 114 413 21 205 111 175 Cuts at: 4412 4492 4859 5244 5261 6713 6799 6811 7157 Size: 80 367 385 17 1452 86 12 346 Cuts at: 7157 1972 Size: 1977 Fragments arranged by size: 1977 1452 870 531 413 385 367 346 205 175 114 111 86 80 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2661 2661 Size: 7162 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5671 Fragments arranged by size: 5671 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3660 487 Size: 683 56 2434 3989 Fragments arranged by size: 3989 2434 683 56 BtsI GCAGTG_nn' Cuts at: 3990 4431 4678 5472 5492 3990 Size: 441 247 794 20 5660 Fragments arranged by size: 5660 794 441 247 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7127 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DraIII CAC_nnn'GTG Cuts at: 4426 4426 Size: 7162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4292 6169 809 Size: 507 2976 1877 1802 Fragments arranged by size: 2976 1877 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4609 5498 7127 Size: 54 1425 50 275 18 2779 889 1629 Cuts at: 7127 8 Size: 43 Fragments arranged by size: 2779 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4609 1537 Size: 275 2797 4090 Fragments arranged by size: 4090 2797 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2367 2495 2796 3170 3207 3227 3360 3447 Size: 1014 128 301 374 37 20 133 87 Cuts at: 3447 4180 5092 1353 Size: 733 912 3423 Fragments arranged by size: 3423 1014 912 733 374 301 133 128 87 37 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5749 6123 6269 387 Size: 1064 138 4160 374 146 1280 Fragments arranged by size: 4160 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2494 2494 Size: 7162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 3938 3980 4254 5225 5822 6609 Size: 393 848 1458 42 274 971 597 787 Cuts at: 6609 6877 1239 Size: 268 1524 Fragments arranged by size: 1524 1458 971 848 787 597 393 274 268 42 EcoNI CCTnn'n_nnAGG Cuts at: 4196 4196 Size: 7162 EcoO109I rG'GnC_Cy Cuts at: 2764 2921 2933 3808 4264 2764 Size: 157 12 875 456 5662 Fragments arranged by size: 5662 875 456 157 12 EcoRI G'AATT_C Cuts at: 4471 4849 4471 Size: 378 6784 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3434 3434 Size: 7162 FspI TGC'GCA Cuts at: 5668 5668 Size: 7162 HaeII r_GCGC'y Cuts at: 1678 6309 1678 Size: 4631 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2270 2276 2302 Size: 32 135 32 494 32 172 6 26 Cuts at: 2302 2308 2948 2980 3206 3238 3263 3295 3380 Size: 6 640 32 226 32 25 32 85 Cuts at: 3380 3412 3566 3598 4004 4036 5803 5835 5877 Size: 32 154 32 406 32 1767 32 42 Cuts at: 5877 5909 1373 Size: 32 2626 Fragments arranged by size: 2626 1767 640 494 406 226 172 154 135 85 42 32 32 32 32 32 32 32 32 32 32 32 26 25 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 4364 4506 4752 669 Size: 988 281 2426 142 246 3079 Fragments arranged by size: 3079 2426 988 281 246 142 HindIII A'AGCT_T Cuts at: 748 748 Size: 7162 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6276 6460 1305 Size: 4971 184 2007 Fragments arranged by size: 4971 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5340 Fragments arranged by size: 5340 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 5120 5479 5638 518 Size: 648 1267 2687 359 159 2042 Fragments arranged by size: 2687 2042 1267 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 4578 5255 6403 6648 1606 Size: 419 2553 677 1148 245 2120 Fragments arranged by size: 2553 2120 1148 677 419 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5671 Fragments arranged by size: 5671 939 552 NdeI CA'TA_TG Cuts at: 387 3066 3570 387 Size: 2679 504 3979 Fragments arranged by size: 3979 2679 504 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 2432 4520 6065 1132 Size: 97 1203 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 1203 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7162 PciI A'CATG_T Cuts at: 6061 6061 Size: 7162 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7162 PfoI T'CCnGG_A Cuts at: 3903 4240 3903 Size: 337 6825 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5231 5263 6744 6776 5231 Size: 32 1481 32 5617 Fragments arranged by size: 5617 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 3808 4264 2764 Size: 1044 456 5662 Fragments arranged by size: 5662 1044 456 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 4732 4732 Size: 7162 PstI C_TGCA'G Cuts at: 830 4514 830 Size: 3684 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 SacI G_AGCT'C Cuts at: 2496 2496 Size: 7162 SalI G'TCGA_C Cuts at: 1936 4362 4504 1936 Size: 2426 142 4594 Fragments arranged by size: 4594 2426 142 SanDI GG'GwC_CC Cuts at: 4264 4264 Size: 7162 SapI GCTCTTCn'nnn_ Cuts at: 2796 2796 Size: 7162 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 5410 7122 5410 Size: 1712 5450 SexAI A'CCwGG_T Cuts at: 2389 2389 Size: 7162 SfcI C'TryA_G Cuts at: 826 1045 3783 4510 5645 6326 6517 826 Size: 219 2738 727 1135 681 191 1471 Fragments arranged by size: 2738 1471 1135 727 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 4489 1872 Size: 2617 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3797 3896 5266 6167 6429 820 Size: 197 926 1854 99 1370 901 262 1553 Fragments arranged by size: 1854 1553 1370 926 901 262 197 99 SnaBI TAC'GTA Cuts at: 493 4953 493 Size: 4460 2702 SpeI A'CTAG_T Cuts at: 152 2162 152 Size: 2010 5152 SphI G_CATG'C Cuts at: 1132 2432 4520 1132 Size: 1300 2088 3774 Fragments arranged by size: 3774 2088 1300 SspI AAT'ATT Cuts at: 5 52 2018 5086 5 Size: 47 1966 3068 2081 Fragments arranged by size: 3068 2081 1966 47 StuI AGG'CCT Cuts at: 1517 4066 1517 Size: 2549 4613 StyI C'CwwG_G Cuts at: 513 1065 2004 2576 2693 2849 4008 4601 513 Size: 552 939 572 117 156 1159 593 3074 Fragments arranged by size: 3074 1159 939 593 572 552 156 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4195 5189 5206 5359 5544 5989 325 Size: 1255 2615 994 17 153 185 445 1498 Fragments arranged by size: 2615 1498 1255 994 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2059 5408 7120 Size: 274 80 33 51 1443 82 3349 1712 Cuts at: 7120 96 Size: 138 Fragments arranged by size: 3349 1712 1443 274 138 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 3376 3408 3885 3917 3969 4001 7122 Size: 32 1160 32 477 32 52 32 3121 Cuts at: 7122 7154 2184 Size: 32 2192 Fragments arranged by size: 3121 2192 1160 477 52 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3677 1316 Size: 2361 4801 XbaI T'CTAG_A Cuts at: 4129 4498 4129 Size: 369 6793 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2039 4189 4309 2039 Size: 2150 120 4892 Fragments arranged by size: 4892 2150 120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7162 XmaI C'CCGG_G Cuts at: 1870 4487 1870 Size: 2617 4545 XmnI GAAnn'nnTTC Cuts at: 2805 5291 2805 Size: 2486 4676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3679 4970 276 Size: 53 83 186 936 2145 1291 2468 Fragments arranged by size: 2468 2145 1291 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI