Restriction map for
pFN21AE4288
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AocI ApaI AscI
Asp718I AspI AviII BalI BamHI
BbeI BcgI BfrI BglI BglII
BsiEI BsiWI BsmBI BspMI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
CpoI DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I FauI FseI
FspI HpaI KasI KpnI KspI
MluI MluNI MscI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 789 TCCGGA T^CCGGA
AcyI 2375 GGCGTC G[GA]^CG[CT]C
Alw44I 4852 GTGCAC G^TGCAC
Aor51HI 2396 AGCGCT AGC^GCT
ApaLI 4852 GTGCAC G^TGCAC
AseI 2521 ATTAAT AT^TAAT
AsnI 2521 ATTAAT AT^TAAT
AvaII 3646 GGACC G^G[AT]CC
AvrII 4099 CCTAGG C^CTAGG
BanI 3245 GGCACC G^G[CT][GA]CC
BbrPI 1325 CACGTG CAC^GTG
BbsI 3563 GTCTTC GAAGAC(2/6)
BlnI 4099 CCTAGG C^CTAGG
BpuAI 3563 GTCTTC GAAGAC(2/6)
BsaAI 1325 CACGTG [CT]AC^GT[GA]
BsaBI 1675 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2375 GGCGTC G[GA]^CG[CT]C
BsaWI 789 TCCGGA [AT]^CCGG[AT]
BseAI 789 TCCGGA T^CCGGA
BspDI 2525 ATCGAT AT^CGAT
BspEI 789 TCCGGA T^CCGGA
BspLU11I 1852 ACATGT A^CATGT
BstAPI 4926 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2525 ATCGAT AT^CGAT
DraI 4711 TTTAAA TTT^AAA
DraII 3645 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 832 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1503 CCATGG C^C[GA][CT]GG
EaeI 3276 TGGCCG [CT]^GGCC[GA]
Eco47III 2396 AGCGCT AGC^GCT
EcoO109I 3645 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1864 GAATTC G^AATTC
EcoRV 2347 GATATC GAT^ATC
HaeII 2396 AGCGCT [GA]GCGC^[CT]
HgaI 2376 GCGTC GACGC(5/10)
Hin1I 2375 GGCGTC G[GA]^CG[CT]C
MamI 1675 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 789 TCCGGA T^CCGGA
NcoI 1503 CCATGG C^CATGG
PacI 4897 TTAATTAA TTAAT^TAA
PmaCI 1325 CACGTG CAC^GTG
PmlI 1325 CACGTG CAC^GTG
PpuMI 3645 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2521 ATTAAT AT^TAAT
PstI 2492 CTGCAG CTGCA^G
SmaI 3863 CCCGGG CCC^GGG
XmaCI 3863 CCCGGG C^CCGGG
XmaI 3863 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 1175 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4338 GACCTGTGGTC
AlwNI 442 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1637 CAGATTCTG
AspEI 1175 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4338 GACCTGTGGTC
BbvCI 2665 CCTCAGC CCTCAGC(-5/-2)
2922 GCTGAGG
BciVI 3254 GGATAC GTATCC(6/5)
2913 GTATCC
BclI 1998 TGATCA T^GATCA
2156 TGATCA
BcnI 3863 CCCGG CC^[GC]GG
3864 CCGGG
BlpI 2874 GCTCAGC GC^T[ACGT]AGC
3086 GCTTAGC
Bpu1102I 2874 GCTCAGC GC^T[ACGT]AGC
3086 GCTTAGC
Bsp1407I 3137 TGTACA T^GTACA
4884 TGTACA
BspHI 719 TCATGA T^CATGA
4014 TCATGA
BsrGI 3137 TGTACA T^GTACA
4884 TGTACA
BssSI 1483 CTCGTG CACGAG(-5/-1)
2018 CTCGTG
BstXI 869 CCATCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCATGACAATGG
CelII 2874 GCTCAGC GC^T[ACGT]AGC
3086 GCTTAGC
CfoI 2397 GCGC GCG^C
3153 GCGC
Eam1105I 1175 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4338 GACCTGTGGTC
EcoT22I 73 ATGCAT ATGCA^T
4365 ATGCAT
FbaI 1998 TGATCA T^GATCA
2156 TGATCA
HapII 790 CCGG C^CGG
3864 CCGG
HhaI 2397 GCGC GCG^C
3153 GCGC
HinP1I 2397 GCGC G^CGC
3153 GCGC
HincII 1766 GTCAAC GT[CT]^[GA]AC
2139 GTCAAC
HindII 1766 GTCAAC GT[CT]^[GA]AC
2139 GTCAAC
HpaII 790 CCGG C^CGG
3864 CCGG
MfeI 245 CAATTG C^AATTG
936 CAATTG
MspA1I 1343 CAGCTG C[AC]G^C[GT]G
3301 CAGCTG
MspI 790 CCGG C^CGG
3864 CCGG
MunI 245 CAATTG C^AATTG
936 CAATTG
NciI 3863 CCCGG CC^[GC]GG
3864 CCGGG
NsiI 73 ATGCAT ATGCA^T
4365 ATGCAT
PvuII 1343 CAGCTG CAG^CTG
3301 CAGCTG
RcaI 719 TCATGA T^CATGA
4014 TCATGA
SspBI 3137 TGTACA T^GTACA
4884 TGTACA
StuI 2936 AGGCCT AGG^CCT
4985 AGGCCT
XcmI 1503 CCATGGTTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2505 CCAGCGTTTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaI
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI