(Linear) (Six-base) MAPSORT of: KIEE4288.seq Check: 1717 from: 1 to: 5049 KIEE4288.orf With 182 enzymes: SgfI * July 3, 2012 18:56 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 525 2053 2107 2890 3365 3550 4192 Size: 346 179 1528 54 783 475 185 642 Cuts at: 4192 4819 5049 Size: 627 230 Fragments arranged by size: 1528 783 642 627 475 346 230 185 179 54 AfeI AGC'GCT Cuts at: 0 2398 5049 Size: 2398 2651 AflIII A'CryG_T Cuts at: 0 1324 1326 1852 5049 Size: 1324 2 526 3197 Fragments arranged by size: 3197 1324 526 2 AhdI GACnn_n'nnGTC Cuts at: 0 1180 4343 5049 Size: 1180 3163 706 Fragments arranged by size: 3163 1180 706 AlwNI CAG_nnn'CTG Cuts at: 0 447 1642 5049 Size: 447 1195 3407 Fragments arranged by size: 3407 1195 447 ApaLI G'TGCA_C Cuts at: 0 4852 5049 Size: 4852 197 ApoI r'AATT_y Cuts at: 0 393 638 690 1080 1106 1288 1598 1864 Size: 393 245 52 390 26 182 310 266 Cuts at: 1864 3451 3946 4660 5049 Size: 1587 495 714 389 Fragments arranged by size: 1587 714 495 393 390 389 310 266 245 182 52 26 AseI AT'TA_AT Cuts at: 0 2522 5049 Size: 2522 2527 AvaI C'yCGr_G Cuts at: 0 1207 2412 3863 5049 Size: 1207 1205 1451 1186 Fragments arranged by size: 1451 1207 1205 1186 AvrII C'CTAG_G Cuts at: 0 4099 5049 Size: 4099 950 BanI G'GyrC_C Cuts at: 0 3245 5049 Size: 3245 1804 BanII G_rGCy'C Cuts at: 0 2419 2868 4811 5049 Size: 2419 449 1943 238 Fragments arranged by size: 2419 1943 449 238 BbsI GAAGACnn'nnnn_ Cuts at: 0 3556 5049 Size: 3556 1493 BbvCI CC'TCA_GC Cuts at: 0 2666 2923 5049 Size: 2666 257 2126 Fragments arranged by size: 2666 2126 257 BciVI GTATCCnnnnn_n' Cuts at: 0 2924 3248 5049 Size: 2924 324 1801 Fragments arranged by size: 2924 1801 324 BclI T'GATC_A Cuts at: 0 1998 2156 5049 Size: 1998 158 2893 Fragments arranged by size: 2893 1998 158 BfrBI ATG'CAT Cuts at: 0 75 4367 5049 Size: 75 4292 682 Fragments arranged by size: 4292 682 75 BlpI GC'TnA_GC Cuts at: 0 2875 3087 5049 Size: 2875 212 1962 Fragments arranged by size: 2875 1962 212 Bme1580I G_kGCm'C Cuts at: 0 990 4856 5049 Size: 990 3866 193 Fragments arranged by size: 3866 990 193 BmrI ACTGGGnnnn_n' Cuts at: 0 2871 5049 Size: 2871 2178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 11 2531 2765 3661 5049 Size: 11 2520 234 896 1388 Fragments arranged by size: 2520 1388 896 234 11 Bpu10I CC'TnA_GC Cuts at: 0 2666 2923 5049 Size: 2666 257 2126 Fragments arranged by size: 2666 2126 257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 699 3304 4241 5049 Size: 699 2605 937 808 Fragments arranged by size: 2605 937 808 699 BsaI GGTCTCn'nnnn_ Cuts at: 0 906 1486 3874 5049 Size: 906 580 2388 1175 Fragments arranged by size: 2388 1175 906 580 BsaAI yAC'GTr Cuts at: 0 1327 5049 Size: 1327 3722 BsaBI GATnn'nnATC Cuts at: 0 1679 5049 Size: 1679 3370 BsaHI Gr'CG_yC Cuts at: 0 2376 5049 Size: 2376 2673 BsaWI w'CCGG_w Cuts at: 0 789 5049 Size: 789 4260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2753 2783 4396 4426 5049 Size: 2753 30 1613 30 623 Fragments arranged by size: 2753 1613 623 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 14 3147 3481 3823 4510 5049 Size: 14 3133 334 342 687 539 Fragments arranged by size: 3133 687 539 342 334 14 BseYI C'CCAG_C Cuts at: 0 2504 3350 5049 Size: 2504 846 1699 Fragments arranged by size: 2504 1699 846 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 976 3391 4735 5049 Size: 359 617 2415 1344 314 Fragments arranged by size: 2415 1344 617 359 314 BsiHKAI G_wGCw'C Cuts at: 0 24 3078 3314 3852 4308 4856 5049 Size: 24 3054 236 538 456 548 193 Fragments arranged by size: 3054 548 538 456 236 193 24 BsmI GAATG_Cn' Cuts at: 0 3095 4369 4928 4977 5049 Size: 3095 1274 559 49 72 Fragments arranged by size: 3095 1274 559 72 49 Bsp1286I G_dGCh'C Cuts at: 0 24 990 2419 2868 3078 3314 3852 4308 Size: 24 966 1429 449 210 236 538 456 Cuts at: 4308 4811 4856 5049 Size: 503 45 193 Fragments arranged by size: 1429 966 538 503 456 449 236 210 193 45 24 BspEI T'CCGG_A Cuts at: 0 789 5049 Size: 789 4260 BspHI T'CATG_A Cuts at: 0 719 4014 5049 Size: 719 3295 1035 Fragments arranged by size: 3295 1035 719 BsrDI GCAATG_nn' Cuts at: 0 2132 2917 4073 4937 5049 Size: 2132 785 1156 864 112 Fragments arranged by size: 2132 1156 864 785 112 BsrGI T'GTAC_A Cuts at: 0 3137 4884 5049 Size: 3137 1747 165 Fragments arranged by size: 3137 1747 165 BssSI C'ACGA_G Cuts at: 0 1483 2018 5049 Size: 1483 535 3031 Fragments arranged by size: 3031 1483 535 BstAPI GCAn_nnn'nTGC Cuts at: 0 4932 5049 Size: 4932 117 BstXI CCAn_nnnn'nTGG Cuts at: 0 876 3000 5049 Size: 876 2124 2049 Fragments arranged by size: 2124 2049 876 BstYI r'GATC_y Cuts at: 0 2444 2568 3975 4399 4575 5049 Size: 2444 124 1407 424 176 474 Fragments arranged by size: 2444 1407 474 424 176 124 BtgI C'CryG_G Cuts at: 0 1503 5049 Size: 1503 3546 BtsI GCAGTG_nn' Cuts at: 0 2112 5049 Size: 2112 2937 ClaI AT'CG_AT Cuts at: 0 2526 5049 Size: 2526 2523 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3248 3283 5049 Size: 3248 35 1766 Fragments arranged by size: 3248 1766 35 DraI TTT'AAA Cuts at: 0 4713 5049 Size: 4713 336 DraIII CAC_nnn'GTG Cuts at: 0 837 5049 Size: 837 4212 EaeI y'GGCC_r Cuts at: 0 3276 5049 Size: 3276 1773 EarI CTCTTCn'nnn_ Cuts at: 0 153 205 251 365 577 767 1208 1541 Size: 153 52 46 114 212 190 441 333 Cuts at: 1541 2231 2999 3284 3587 5033 5049 Size: 690 768 285 303 1446 16 Fragments arranged by size: 1446 768 690 441 333 303 285 212 190 153 114 52 46 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4109 5049 Size: 4109 940 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 11 346 525 2053 2107 2531 2765 2890 Size: 11 335 179 1528 54 424 234 125 Cuts at: 2890 3365 3550 3661 4192 4819 5049 Size: 475 185 111 531 627 230 Fragments arranged by size: 1528 627 531 475 424 335 234 230 185 179 125 111 54 11 EcoO109I rG'GnC_Cy Cuts at: 0 3646 5049 Size: 3646 1403 EcoRI G'AATT_C Cuts at: 0 1864 5049 Size: 1864 3185 EcoRV GAT'ATC Cuts at: 0 2349 5049 Size: 2349 2700 HaeII r_GCGC'y Cuts at: 0 2400 5049 Size: 2400 2649 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 917 949 1155 1166 1187 1198 1364 1396 Size: 917 32 206 11 21 11 166 32 Cuts at: 1396 2148 2180 2519 2551 3695 3727 3745 3777 Size: 752 32 339 32 1144 32 18 32 Cuts at: 3777 4252 4284 4329 4361 4431 4463 4760 4792 Size: 475 32 45 32 70 32 297 32 Cuts at: 4792 5049 Size: 257 Fragments arranged by size: 1144 917 752 475 339 297 257 206 166 70 45 32 32 32 32 32 32 32 32 32 32 21 18 11 11 HincII GTy'rAC Cuts at: 0 1768 2141 5049 Size: 1768 373 2908 Fragments arranged by size: 2908 1768 373 HindIII A'AGCT_T Cuts at: 0 1735 3356 3526 5049 Size: 1735 1621 170 1523 Fragments arranged by size: 1735 1621 1523 170 MfeI C'AATT_G Cuts at: 0 245 936 5049 Size: 245 691 4113 Fragments arranged by size: 4113 691 245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 615 749 3717 3800 5049 Size: 615 134 2968 83 1249 Fragments arranged by size: 2968 1249 615 134 83 MslI CAynn'nnrTG Cuts at: 0 874 2998 3970 4507 5049 Size: 874 2124 972 537 542 Fragments arranged by size: 2124 972 874 542 537 MspA1I CmG'CkG Cuts at: 0 1345 3303 5049 Size: 1345 1958 1746 Fragments arranged by size: 1958 1746 1345 NcoI C'CATG_G Cuts at: 0 1503 5049 Size: 1503 3546 NsiI A_TGCA'T Cuts at: 0 77 4369 5049 Size: 77 4292 680 Fragments arranged by size: 4292 680 77 NspI r_CATG'y Cuts at: 0 1856 2056 2212 5049 Size: 1856 200 156 2837 Fragments arranged by size: 2837 1856 200 156 PacI TTA_AT'TAA Cuts at: 0 4901 5049 Size: 4901 148 PasI CC'CwG_GG Cuts at: 0 3250 5049 Size: 3250 1799 PciI A'CATG_T Cuts at: 0 1852 5049 Size: 1852 3197 PflMI CCAn_nnn'nTGG Cuts at: 0 1084 2190 2832 5049 Size: 1084 1106 642 2217 Fragments arranged by size: 2217 1106 1084 642 PfoI T'CCnGG_A Cuts at: 0 331 1705 5049 Size: 331 1374 3344 Fragments arranged by size: 3344 1374 331 PmlI CAC'GTG Cuts at: 0 1327 5049 Size: 1327 3722 PpuMI rG'GwC_Cy Cuts at: 0 3646 5049 Size: 3646 1403 PsiI TTA'TAA Cuts at: 0 1700 5049 Size: 1700 3349 PstI C_TGCA'G Cuts at: 0 2496 5049 Size: 2496 2553 PvuII CAG'CTG Cuts at: 0 1345 3303 5049 Size: 1345 1958 1746 Fragments arranged by size: 1958 1746 1345 SapI GCTCTTCn'nnn_ Cuts at: 0 2999 3587 5033 5049 Size: 2999 588 1446 16 Fragments arranged by size: 2999 1446 588 16 SfcI C'TryA_G Cuts at: 0 2492 2676 4073 5049 Size: 2492 184 1397 976 Fragments arranged by size: 2492 1397 976 184 SmaI CCC'GGG Cuts at: 0 3865 5049 Size: 3865 1184 SmlI C'TyrA_G Cuts at: 0 678 3283 4256 5049 Size: 678 2605 973 793 Fragments arranged by size: 2605 973 793 678 SspI AAT'ATT Cuts at: 0 300 3803 4021 4734 5049 Size: 300 3503 218 713 315 Fragments arranged by size: 3503 713 315 300 218 StuI AGG'CCT Cuts at: 0 2938 4987 5049 Size: 2938 2049 62 Fragments arranged by size: 2938 2049 62 StyI C'CwwG_G Cuts at: 0 1503 1591 3122 3418 4099 5049 Size: 1503 88 1531 296 681 950 Fragments arranged by size: 1531 1503 950 681 296 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2178 2963 3263 5049 Size: 2178 785 300 1786 Fragments arranged by size: 2178 1786 785 300 TatI w'GTAC_w Cuts at: 0 1849 2319 2550 3137 4884 5049 Size: 1849 470 231 587 1747 165 Fragments arranged by size: 1849 1747 587 470 231 165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1124 1156 1205 1237 4396 4428 5049 Size: 1124 32 49 32 3159 32 621 Fragments arranged by size: 3159 1124 621 49 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1510 2512 5049 Size: 1510 1002 2537 Fragments arranged by size: 2537 1510 1002 XmaI C'CCGG_G Cuts at: 0 3863 5049 Size: 3863 1186 XmnI GAAnn'nnTTC Cuts at: 0 706 1307 3966 5049 Size: 706 601 2659 1083 Fragments arranged by size: 2659 1083 706 601 Enzymes that do cut: AcuI AfeI AflIII AhdI AlwNI ApaLI ApoI AseI AvaI AvrII BanI BanII BbsI BbvCI BciVI BclI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI CspCI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NsiI NspI PacI PasI PciI PflMI PfoI PmlI PpuMI PsiI PstI PvuII SapI SfcI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AleI AlfI AloI ApaI AscI AsiSI BaeI BamHI BbeI BcgI BglI BglII BmgBI BmtI BplI BsiEI BsiWI BsmBI BspMI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI DrdI EagI EcoICRI EcoNI FalI FseI FspI FspAI HpaI KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI ZraI