################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AvaI AviII BamHI BbeI BbvCI BcgI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoNI EcoRV FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AccII 730 CGCG CG^CG AccIII 918 TCCGGA T^CCGGA AcyI 1057 GGCGTC G[GA]^CG[CT]C AhdI 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 314 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1603 CCTCAGG CC^T[ACGT]AGG ApaI 912 GGGCCC GGGCC^C AspEI 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 781 CCTAGG C^CTAGG BalI 1247 TGGCCA TGG^CCA BanI 1080 GGTGCC G^G[CT][GA]CC BbrPI 1159 CACGTG CAC^GTG BbsI 1561 GAAGAC GAAGAC(2/6) BciVI 154 GGATAC GTATCC(6/5) BclI 824 TGATCA T^GATCA BcnI 72 CCGGG CC^[GC]GG BlnI 781 CCTAGG C^CTAGG BpuAI 1561 GAAGAC GAAGAC(2/6) BsaAI 1159 CACGTG [CT]AC^GT[GA] BsaHI 1057 GGCGTC G[GA]^CG[CT]C BseAI 918 TCCGGA T^CCGGA BsmAI 980 GAGAC GTCTC(1/5) BsmBI 980 GAGACG CGTCTC(1/5) BspEI 918 TCCGGA T^CCGGA BspHI 590 TCATGA T^CATGA BsrBI 568 GAGCGG CCGCTC(-3/-3) BsrDI 608 CATTGC GCAATG(2/0) BstEII 193 GGTAACC G^GT[ACGT]ACC BstPI 193 GGTAACC G^GT[ACGT]ACC BstUI 730 CGCG CG^CG BstYI 833 AGATCC [GA]^GATC[CT] Bsu36I 1603 CCTCAGG CC^T[ACGT]AGG DraI 655 TTTAAA TTT^AAA Eam1105I 1655 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1603 CCTCAGG CC^T[ACGT]AGG EcoO65I 193 GGTAACC G^GT[ACGT]ACC EcoRI 241 GAATTC G^AATTC EcoT22I 650 ATGCAT ATGCA^T FbaI 824 TGATCA T^GATCA HgaI 1058 GCGTC GACGC(5/10) Hin1I 1057 GGCGTC G[GA]^CG[CT]C MflI 833 AGATCC [GA]^GATC[CT] MluNI 1247 TGGCCA TGG^CCA MroI 918 TCCGGA T^CCGGA MscI 1247 TGGCCA TGG^CCA MseI 656 TTAA T^TAA MslI 1422 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 438 CCGCTG C[AC]G^C[GT]G MvnI 730 CGCG CG^CG NciI 72 CCGGG CC^[GC]GG NsiI 650 ATGCAT ATGCA^T NspI 933 GCATGC [GA]CATG^[CT] PflFI 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1159 CACGTG CAC^GTG PmlI 1159 CACGTG CAC^GTG PpuMI 1502 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 436 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 590 TCATGA T^CATGA SacI 1368 GAGCTC GAGCT^C SfcI 1545 CTACAG C^T[GA][CT]AG SphI 933 GCATGC GCATG^C SspI 1646 AATATT AAT^ATT Tru9I 656 TTAA T^TAA Tsp45I 1583 GTCAC ^GT[GC]AC TspRI 642 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1310 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 833 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 241 GAATTC [GA]^AATT[CT] 329 AAATTC Aor51HI 25 AGCGCT AGC^GCT 1594 AGCGCT ApoI 241 GAATTC [GA]^AATT[CT] 329 AAATTC AspHI 1292 GAGCAC G[AT]GC[AT]^C 1368 GAGCTC BsgI 67 CTGCAC GTGCAG(16/14) 13 GTGCAG BsiHKAI 1292 GAGCAC G[AT]GC[AT]^C 1368 GAGCTC DdeI 144 CTCAG C^T[ACGT]AG 1604 CTCAG DsaI 614 CCACGG C^C[GA][CT]GG 1026 CCGTGG EaeI 1023 TGGCCG [CT]^GGCC[GA] 1247 TGGCCA EarI 632 CTCTTC CTCTTC(1/4) 1013 GAAGAG Eco47III 25 AGCGCT AGC^GCT 1594 AGCGCT FauI 508 CCCGC CCCGC(4/6) 1277 GCGGG HaeII 25 AGCGCT [GA]GCGC^[CT] 1594 AGCGCT Ksp632I 632 CTCTTC CTCTTC(1/4) 1013 GAAGAG MaeII 1160 ACGT A^CGT 1681 ACGT MaeIII 194 GTAAC ^GT[ACGT]AC 1583 GTCAC PflMI 871 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 903 CCAGGTCATGG PleI 1314 GAGTC GAGTC(4/5) 1581 GAGTC StuI 993 AGGCCT AGG^CCT 1553 AGGCCT Van91I 871 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 903 CCAGGTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp509I TthHB8I XcmI