(Circular) (Six-base) MAPSORT of: pFN21AE4144.seq Check: 3616 from: 1 to: 6421 pFN21AE4144 6421 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3346 3346 Size: 6421 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4231 278 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3764 1937 Size: 1827 4594 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2331 3158 4484 5868 6136 1632 Size: 699 827 1326 1384 268 1917 Fragments arranged by size: 1917 1384 1326 827 699 268 AfeI AGC'GCT Cuts at: 2032 3601 2032 Size: 1569 4852 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 5320 6297 1203 Size: 4117 977 1327 Fragments arranged by size: 4117 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 3665 5150 3665 Size: 1485 4936 AleI CACnn'nnGTG Cuts at: 1166 3431 1166 Size: 2265 4156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2109 1184 Size: 925 5496 AlwNI CAG_nnn'CTG Cuts at: 2324 4164 5736 2324 Size: 1840 1572 3009 Fragments arranged by size: 3009 1840 1572 ApaI G_GGCC'C Cuts at: 2921 2921 Size: 6421 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 2246 2334 3730 3956 4108 6217 1493 Size: 753 88 1396 226 152 2109 1697 Fragments arranged by size: 2109 1697 1396 753 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 3746 1870 Size: 73 1803 4545 Fragments arranged by size: 4545 1803 73 AvrII C'CTAG_G Cuts at: 2786 2786 Size: 6421 BamHI G'GATC_C Cuts at: 3751 4118 3751 Size: 367 6054 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 3742 5197 618 Size: 325 217 627 1298 657 1455 1842 Fragments arranged by size: 1842 1455 1298 657 627 325 217 BanII G_rGCy'C Cuts at: 1391 2921 3055 3377 3740 1391 Size: 1530 134 322 363 4072 Fragments arranged by size: 4072 1530 363 322 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3573 928 Size: 971 1674 3776 Fragments arranged by size: 3776 1674 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4612 4646 1903 Size: 34 2675 34 3678 Fragments arranged by size: 3678 2675 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2153 4313 5523 2153 Size: 2160 1210 3051 Fragments arranged by size: 3051 2160 1210 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 5140 BfrBI ATG'CAT Cuts at: 2657 2657 Size: 6421 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5032 136 Size: 107 122 71 631 605 3360 1525 Fragments arranged by size: 3360 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 3838 3838 Size: 6421 Bme1580I G_kGCm'C Cuts at: 1790 2921 4482 5638 1790 Size: 1131 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 1131 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4152 5110 442 Size: 416 53 83 399 24 2735 958 1753 Fragments arranged by size: 2735 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3459 3459 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2670 3061 3324 5081 1239 Size: 1431 391 263 1757 2579 Fragments arranged by size: 2579 1757 1431 391 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4546 5411 5709 4546 Size: 865 298 5258 Fragments arranged by size: 5258 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5084 882 Size: 617 3585 2219 Fragments arranged by size: 3585 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3166 4212 493 Size: 711 1962 1046 2702 Fragments arranged by size: 2702 1962 1046 711 BsaBI GATnn'nnATC Cuts at: 6415 6415 Size: 6421 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3063 4228 4610 5245 Size: 53 83 186 936 1530 1165 382 635 Cuts at: 5245 275 Size: 1451 Fragments arranged by size: 1530 1451 1165 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2387 2923 3377 3625 4854 5252 5526 5673 6084 Size: 536 454 248 1229 398 274 147 411 Cuts at: 6084 2387 Size: 2724 Fragments arranged by size: 2724 1229 536 454 411 398 274 248 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3349 3364 3379 3394 1178 Size: 30 165 30 1946 15 15 15 4205 Fragments arranged by size: 4205 1946 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2559 2775 3597 1186 Size: 1373 216 822 4010 Fragments arranged by size: 4010 1373 822 216 BseYI C'CCAG_C Cuts at: 3237 3465 5624 6120 3237 Size: 228 2159 496 3538 Fragments arranged by size: 3538 2159 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2057 1163 Size: 573 303 18 5527 Fragments arranged by size: 5527 573 303 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3871 4632 5660 1540 Size: 275 186 1870 761 1028 2301 Fragments arranged by size: 2301 1870 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3301 3377 4482 4567 5638 3301 Size: 76 1105 85 1071 4084 Fragments arranged by size: 4084 1105 1071 85 76 BsmI GAATG_Cn' Cuts at: 3932 4025 3932 Size: 93 6328 BsmBI CGTCTCn'nnnn_ Cuts at: 2979 2979 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2921 3055 3301 3377 3740 4482 4567 Size: 399 1131 134 246 76 363 742 85 Cuts at: 4567 5638 1391 Size: 1071 2174 Fragments arranged by size: 2174 1131 1071 742 399 363 246 134 85 76 BspEI T'CCGG_A Cuts at: 2923 6084 2923 Size: 3161 3260 BspHI T'CATG_A Cuts at: 2595 4310 6040 2595 Size: 1715 1730 2976 Fragments arranged by size: 2976 1730 1715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3216 3346 3496 3655 3709 3776 844 Size: 2372 130 150 159 54 67 3489 Fragments arranged by size: 3489 2372 159 150 130 67 54 BsrBI CCG'CTC Cuts at: 1394 1823 2575 4308 1394 Size: 429 752 1733 3507 Fragments arranged by size: 3507 1733 752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2612 4916 5090 65 Size: 1183 1364 2304 174 1396 Fragments arranged by size: 2304 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5065 5252 1700 Size: 110 3255 187 2869 Fragments arranged by size: 3255 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6421 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 4481 5493 4481 Size: 1012 5409 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6421 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 2198 1184 Size: 1014 5407 BstYI r'GATC_y Cuts at: 1972 2838 3751 4118 4503 4520 5972 6058 6070 Size: 866 913 367 385 17 1452 86 12 Cuts at: 6070 6416 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 913 866 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3609 3609 Size: 6421 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 3031 513 Size: 552 939 615 412 3903 Fragments arranged by size: 3903 939 615 552 412 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5682 Fragments arranged by size: 5682 683 56 BtsI GCAGTG_nn' Cuts at: 3937 4731 4751 3937 Size: 794 20 5607 Fragments arranged by size: 5607 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 2662 3725 2662 Size: 1063 5358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5428 809 Size: 507 4112 1802 Fragments arranged by size: 4112 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3028 3252 3868 Size: 54 1425 50 275 18 1198 224 616 Cuts at: 3868 4757 6386 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1198 889 616 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2643 3013 4351 1353 Size: 1290 370 1338 3423 Fragments arranged by size: 3423 1338 1290 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5008 5382 5528 387 Size: 1064 138 435 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3375 3375 Size: 6421 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2670 3061 3158 3324 4484 5081 Size: 393 699 339 391 97 166 1160 597 Cuts at: 5081 5868 6136 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1160 787 699 597 393 391 339 268 166 97 EcoO109I rG'GnC_Cy Cuts at: 2211 2373 2918 3508 2211 Size: 162 545 590 5124 Fragments arranged by size: 5124 590 545 162 EcoRI G'AATT_C Cuts at: 2246 3730 4108 2246 Size: 1484 378 4559 Fragments arranged by size: 4559 1484 378 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 2034 3603 5568 1678 Size: 356 1569 1965 2531 Fragments arranged by size: 2531 1965 1569 356 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 2732 2764 3207 Size: 32 135 32 693 32 435 32 443 Cuts at: 3207 3239 3651 3683 5062 5094 5136 5168 1373 Size: 32 412 32 1379 32 42 32 2626 Fragments arranged by size: 2626 1379 693 443 435 412 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3765 4011 669 Size: 988 281 1827 246 3079 Fragments arranged by size: 3079 1827 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2251 5535 5719 1305 Size: 946 3284 184 2007 Fragments arranged by size: 3284 2007 946 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3254 10 Size: 54 1425 343 1422 3177 Fragments arranged by size: 3177 1425 1422 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3431 4379 4738 4897 518 Size: 648 2265 948 359 159 2042 Fragments arranged by size: 2265 2042 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2445 3837 4514 5662 5907 1606 Size: 839 1392 677 1148 245 2120 Fragments arranged by size: 2120 1392 1148 839 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4930 Fragments arranged by size: 4930 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NsiI A_TGCA'T Cuts at: 2659 2659 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 2942 3779 5324 1132 Size: 97 1713 837 1545 2229 Fragments arranged by size: 2229 1713 1545 837 97 PasI CC'CwG_GG Cuts at: 1348 3460 1348 Size: 2112 4309 PciI A'CATG_T Cuts at: 5320 5320 Size: 6421 PflMI CCAn_nnn'nTGG Cuts at: 1108 2882 2914 1108 Size: 1774 32 4615 Fragments arranged by size: 4615 1774 32 PfoI T'CCnGG_A Cuts at: 2496 2601 3066 2496 Size: 105 465 5851 Fragments arranged by size: 5851 465 105 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PmlI CAC'GTG Cuts at: 3166 3166 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3508 3508 Size: 6421 PshAI GACnn'nnGTC Cuts at: 1613 2445 1613 Size: 832 5589 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspOMI G'GGCC_C Cuts at: 2917 2917 Size: 6421 PstI C_TGCA'G Cuts at: 830 3773 830 Size: 2943 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6421 SacI G_AGCT'C Cuts at: 3377 3377 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 4669 6381 4669 Size: 1712 4709 SfcI C'TryA_G Cuts at: 826 1045 3550 3769 4904 5585 5776 826 Size: 219 2505 219 1135 681 191 1471 Fragments arranged by size: 2505 1471 1135 681 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4525 5426 5688 820 Size: 197 926 2582 901 262 1553 Fragments arranged by size: 2582 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 2942 3779 1132 Size: 1810 837 3774 Fragments arranged by size: 3774 1810 837 SspI AAT'ATT Cuts at: 5 52 3653 4345 5 Size: 47 3601 692 2081 Fragments arranged by size: 3601 2081 692 47 StuI AGG'CCT Cuts at: 1517 3000 3560 1517 Size: 1483 560 4378 Fragments arranged by size: 4378 1483 560 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2214 2786 3368 3402 3860 Size: 552 939 177 33 572 582 34 458 Cuts at: 3860 513 Size: 3074 Fragments arranged by size: 3074 939 582 572 552 458 177 34 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4448 4465 4618 4803 5248 325 Size: 1255 2868 17 153 185 445 1498 Fragments arranged by size: 2868 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 2380 2665 Size: 274 80 33 51 1443 190 213 285 Cuts at: 2665 4667 6379 96 Size: 2002 1712 138 Fragments arranged by size: 2002 1712 1443 285 274 213 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 3492 3524 6381 6413 2421 Size: 32 1039 32 2857 32 2429 Fragments arranged by size: 2857 2429 1039 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3318 1316 Size: 2002 4419 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2137 2422 2849 2011 Size: 126 285 427 5583 Fragments arranged by size: 5583 427 285 126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6421 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 4550 4550 Size: 6421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4229 276 Size: 53 83 186 936 2695 2468 Fragments arranged by size: 2695 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI