(Linear) (Six-base) MAPSORT of: KIEE4144.seq Check: 6302 from: 1 to: 1716 KIEE4144.orf With 182 enzymes: SgfI * July 3, 2012 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1341 1716 Size: 1341 375 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 1153 1716 Size: 326 827 563 Fragments arranged by size: 827 563 326 AfeI AGC'GCT Cuts at: 0 27 1596 1716 Size: 27 1569 120 Fragments arranged by size: 1569 120 27 AhdI GACnn_n'nnGTC Cuts at: 0 1660 1716 Size: 1660 56 AleI CACnn'nnGTG Cuts at: 0 1426 1716 Size: 1426 290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 104 1716 Size: 104 1612 AlwNI CAG_nnn'CTG Cuts at: 0 319 1716 Size: 319 1397 ApaI G_GGCC'C Cuts at: 0 916 1716 Size: 916 800 ApoI r'AATT_y Cuts at: 0 241 329 1716 Size: 241 88 1387 Fragments arranged by size: 1387 241 88 AvrII C'CTAG_G Cuts at: 0 781 1716 Size: 781 935 BanI G'GyrC_C Cuts at: 0 1080 1716 Size: 1080 636 BanII G_rGCy'C Cuts at: 0 916 1050 1372 1716 Size: 916 134 322 344 Fragments arranged by size: 916 344 322 134 BbsI GAAGACnn'nnnn_ Cuts at: 0 1568 1716 Size: 1568 148 BciVI GTATCCnnnnn_n' Cuts at: 0 148 1716 Size: 148 1568 BclI T'GATC_A Cuts at: 0 824 1716 Size: 824 892 BfrBI ATG'CAT Cuts at: 0 652 1716 Size: 652 1064 Bme1580I G_kGCm'C Cuts at: 0 916 1716 Size: 916 800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1454 1716 Size: 1454 262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 665 1056 1319 1716 Size: 665 391 263 397 Fragments arranged by size: 665 397 391 263 BsaAI yAC'GTr Cuts at: 0 1161 1716 Size: 1161 555 BsaHI Gr'CG_yC Cuts at: 0 1058 1716 Size: 1058 658 BsaWI w'CCGG_w Cuts at: 0 382 918 1372 1620 1716 Size: 382 536 454 248 96 Fragments arranged by size: 536 454 382 248 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1344 1359 1374 1389 1716 Size: 1344 15 15 15 327 Fragments arranged by size: 1344 327 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 554 770 1592 1716 Size: 554 216 822 124 Fragments arranged by size: 822 554 216 124 BseYI C'CCAG_C Cuts at: 0 1232 1460 1716 Size: 1232 228 256 Fragments arranged by size: 1232 256 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 52 1716 Size: 34 18 1664 Fragments arranged by size: 1664 34 18 BsiHKAI G_wGCw'C Cuts at: 0 1296 1372 1716 Size: 1296 76 344 Fragments arranged by size: 1296 344 76 BsmBI CGTCTCn'nnnn_ Cuts at: 0 974 1716 Size: 974 742 Bsp1286I G_dGCh'C Cuts at: 0 916 1050 1296 1372 1716 Size: 916 134 246 76 344 Fragments arranged by size: 916 344 246 134 76 BspEI T'CCGG_A Cuts at: 0 918 1716 Size: 918 798 BspHI T'CATG_A Cuts at: 0 590 1716 Size: 590 1126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1211 1341 1491 1650 1716 Size: 1211 130 150 159 66 Fragments arranged by size: 1211 159 150 130 66 BsrBI CCG'CTC Cuts at: 0 570 1716 Size: 570 1146 BsrDI GCAATG_nn' Cuts at: 0 607 1716 Size: 607 1109 BstEII G'GTnAC_C Cuts at: 0 193 1716 Size: 193 1523 BstYI r'GATC_y Cuts at: 0 833 1716 Size: 833 883 Bsu36I CC'TnA_GG Cuts at: 0 1604 1716 Size: 1604 112 BtgI C'CryG_G Cuts at: 0 614 1026 1716 Size: 614 412 690 Fragments arranged by size: 690 614 412 DraI TTT'AAA Cuts at: 0 657 1716 Size: 657 1059 EaeI y'GGCC_r Cuts at: 0 1023 1247 1716 Size: 1023 224 469 Fragments arranged by size: 1023 469 224 EarI CTCTTCn'nnn_ Cuts at: 0 638 1008 1716 Size: 638 370 708 Fragments arranged by size: 708 638 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1716 Size: 19 1697 EcoICRI GAG'CTC Cuts at: 0 1370 1716 Size: 1370 346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 326 665 1056 1153 1319 1716 Size: 326 339 391 97 166 397 Fragments arranged by size: 397 391 339 326 166 97 EcoO109I rG'GnC_Cy Cuts at: 0 206 368 913 1503 1716 Size: 206 162 545 590 213 Fragments arranged by size: 590 545 213 206 162 EcoRI G'AATT_C Cuts at: 0 241 1716 Size: 241 1475 HaeII r_GCGC'y Cuts at: 0 29 1598 1716 Size: 29 1569 118 Fragments arranged by size: 1569 118 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 260 292 727 759 1202 1234 1646 1678 Size: 260 32 435 32 443 32 412 32 Cuts at: 1678 1716 Size: 38 Fragments arranged by size: 443 435 412 260 38 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 246 1716 Size: 246 1470 MscI TGG'CCA Cuts at: 0 1249 1716 Size: 1249 467 MslI CAynn'nnrTG Cuts at: 0 1426 1716 Size: 1426 290 MspA1I CmG'CkG Cuts at: 0 440 1716 Size: 440 1276 NsiI A_TGCA'T Cuts at: 0 654 1716 Size: 654 1062 NspI r_CATG'y Cuts at: 0 937 1716 Size: 937 779 PasI CC'CwG_GG Cuts at: 0 1455 1716 Size: 1455 261 PflMI CCAn_nnn'nTGG Cuts at: 0 877 909 1716 Size: 877 32 807 Fragments arranged by size: 877 807 32 PfoI T'CCnGG_A Cuts at: 0 491 596 1061 1716 Size: 491 105 465 655 Fragments arranged by size: 655 491 465 105 PmlI CAC'GTG Cuts at: 0 1161 1716 Size: 1161 555 PpuMI rG'GwC_Cy Cuts at: 0 1503 1716 Size: 1503 213 PshAI GACnn'nnGTC Cuts at: 0 440 1716 Size: 440 1276 PspOMI G'GGCC_C Cuts at: 0 912 1716 Size: 912 804 SacI G_AGCT'C Cuts at: 0 1372 1716 Size: 1372 344 SfcI C'TryA_G Cuts at: 0 1545 1716 Size: 1545 171 SphI G_CATG'C Cuts at: 0 937 1716 Size: 937 779 SspI AAT'ATT Cuts at: 0 1648 1716 Size: 1648 68 StuI AGG'CCT Cuts at: 0 995 1555 1716 Size: 995 560 161 Fragments arranged by size: 995 560 161 StyI C'CwwG_G Cuts at: 0 176 209 781 1363 1397 1716 Size: 176 33 572 582 34 319 Fragments arranged by size: 582 572 319 176 34 33 TatI w'GTAC_w Cuts at: 0 162 375 660 1716 Size: 162 213 285 1056 Fragments arranged by size: 1056 285 213 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 416 448 1487 1519 1716 Size: 416 32 1039 32 197 Fragments arranged by size: 1039 416 197 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1313 1716 Size: 1313 403 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 132 417 844 1716 Size: 132 285 427 872 Fragments arranged by size: 872 427 285 132 Enzymes that do cut: AarI AcuI AfeI AhdI AleI AlfI AlwNI ApaI ApoI AvrII BanI BanII BbsI BciVI BclI BfrBI Bme1580I BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstEII BstYI Bsu36I BtgI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MscI MslI MspA1I NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI SacI SfcI SphI SspI StuI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvaI BaeI BamHI BbeI BbvCI BcgI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PpiI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SwaI TaqII XbaI XhoI XmaI XmnI ZraI