################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccII AccIII AclI AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspEI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CpoI DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FauI FbaI FseI FspI HincII HindII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### Acc65I 869 GGTACC G^GTACC AcyI 1193 GGCGTC G[GA]^CG[CT]C AflII 1318 CTTAAG C^TTAAG AflIII 759 ACATGT A^C[GA][CT]GT Aor51HI 515 AGCGCT AGC^GCT Asp718I 869 GGTACC G^GTACC BanI 869 GGTACC G^G[CT][GA]CC BanII 748 GAGCTC G[GA]GC[CT]^C BfrI 1318 CTTAAG C^TTAAG BlpI 1036 GCTAAGC GC^T[ACGT]AGC BpmI 157 CTCCAG CTGGAG(16/14) Bpu1102I 1036 GCTAAGC GC^T[ACGT]AGC BsaAI 1576 CACGTA [CT]AC^GT[GA] BsaHI 1193 GGCGTC G[GA]^CG[CT]C BsgI 1454 GTGCAG GTGCAG(16/14) Bsp1407I 405 TGTACA T^GTACA BspDI 1295 ATCGAT AT^CGAT BspHI 108 TCATGA T^CATGA BspLU11I 759 ACATGT A^CATGT BspMI 917 GCAGGT ACCTGC(4/8) BsrDI 1729 GCAATG GCAATG(2/0) BsrFI 1519 GCCGGT [GA]^CCGG[CT] BsrGI 405 TGTACA T^GTACA BssKI 1644 CCTGG ^CC[ACGT]GG BssSI 745 CACGAG CACGAG(-5/-1) BstAPI 1069 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 1644 CCTGG CC^[AT]GG BstYI 36 GGATCT [GA]^GATC[CT] CelII 1036 GCTAAGC GC^T[ACGT]AGC CfoI 516 GCGC GCG^C Cfr10I 1519 GCCGGT [GA]^CCGG[CT] ClaI 1295 ATCGAT AT^CGAT DraI 1792 TTTAAA TTT^AAA DraII 1383 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 993 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 515 AGCGCT AGC^GCT EcoNI 1009 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1383 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 828 GAATTC G^AATTC EcoRII 1644 CCTGG ^CC[AT]GG EcoT22I 458 ATGCAT ATGCA^T HaeII 515 AGCGCT [GA]GCGC^[CT] HapII 1520 CCGG C^CGG HhaI 516 GCGC GCG^C Hin1I 1193 GGCGTC G[GA]^CG[CT]C HinP1I 516 GCGC G^CGC HindIII 1039 AAGCTT A^AGCTT HpaII 1520 CCGG C^CGG KpnI 869 GGTACC GGTAC^C MaeII 1577 ACGT A^CGT MflI 36 GGATCT [GA]^GATC[CT] MspA1I 1487 CAGCTG C[AC]G^C[GT]G MspI 1520 CCGG C^CGG MvaI 1644 CCTGG CC^[AT]GG NsiI 458 ATGCAT ATGCA^T PflMI 1436 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 767 GACTC GAGTC(4/5) PpuMI 1383 AGGACCT [GA]G^G[AT]CC[CT] PstI 1340 CTGCAG CTGCA^G PvuII 1487 CAGCTG CAG^CTG RcaI 108 TCATGA T^CATGA SacI 748 GAGCTC GAGCT^C ScrFI 1644 CCTGG CC^[ACGT]GG SspBI 405 TGTACA T^GTACA TaqI 1296 TCGA T^CGA TthHB8I 1296 TCGA T^CGA Van91I 1436 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 36 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 186 CCGC CCGC(-3/-1) 1649 GCGG Asp700I 604 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1584 GAATACATTC BbsI 731 GAAGAC GAAGAC(2/6) 1652 GAAGAC BciVI 592 GTATCC GTATCC(6/5) 1492 GTATCC BfaI 908 CTAG C^TAG 1349 CTAG BpuAI 731 GAAGAC GAAGAC(2/6) 1652 GAAGAC BseRI 478 GAGGAG GAGGAG(10/8) 676 GAGGAG BsiYI 1009 CCTGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1436 CCAATTCTTGG BslI 1009 CCTGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1436 CCAATTCTTGG BsmAI 421 GAGAC GTCTC(1/5) 765 GAGAC BsmFI 15 GGGAC GGGAC(10/14) 897 GTCCC BstXI 467 CCAAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 873 CCACATACTTGG EarI 126 CTCTTC CTCTTC(1/4) 547 CTCTTC HaeIII 911 GGCC GG^CC 1739 GGCC HgaI 17 GACGC GACGC(5/10) 1194 GCGTC HphI 384 GGTGA GGTGA(8/7) 939 GGTGA Ksp632I 126 CTCTTC CTCTTC(1/4) 547 CTCTTC MaeI 908 CTAG C^TAG 1349 CTAG MfeI 817 CAATTG C^AATTG 1466 CAATTG MslI 207 CATTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1753 CATGGCTGTG MunI 817 CAATTG C^AATTG 1466 CAATTG MwoI 1069 GCAGTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1352 GCTGTGTAGGC NlaIV 87 GGACCC GG[ACGT]^[ACGT]CC 869 GGTACC NspI 460 GCATGT [GA]CATG^[CT] 759 ACATGT SmlI 613 CTTGAG C^T[CT][GA]AG 1318 CTTAAG SspI 9 AATATT AAT^ATT 1153 AATATT XmnI 604 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1584 GAATACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BmyI BsaJI BsiHKAI Bsp1286I BsrI BstF5I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HinfI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI Sau96I SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI