(Linear) (Six-base) MAPSORT of: KIEE4159.seq Check: 3736 from: 1 to: 1800 KIEE4159.orf With 182 enzymes: SgfI * July 3, 2012 18:40 .. Acc65I G'GTAC_C Cuts at: 0 869 1800 Size: 869 931 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 255 362 1325 1800 Size: 113 142 107 963 475 Fragments arranged by size: 963 475 142 113 107 AfeI AGC'GCT Cuts at: 0 517 1800 Size: 517 1283 AflII C'TTAA_G Cuts at: 0 1318 1800 Size: 1318 482 AflIII A'CryG_T Cuts at: 0 759 1800 Size: 759 1041 ApoI r'AATT_y Cuts at: 0 172 226 564 607 828 1048 1800 Size: 172 54 338 43 221 220 752 Fragments arranged by size: 752 338 221 220 172 54 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1475 1508 1800 Size: 1475 33 292 Fragments arranged by size: 1475 292 33 BanI G'GyrC_C Cuts at: 0 869 1800 Size: 869 931 BanII G_rGCy'C Cuts at: 0 752 1800 Size: 752 1048 BbsI GAAGACnn'nnnn_ Cuts at: 0 738 1659 1800 Size: 738 921 141 Fragments arranged by size: 921 738 141 BciVI GTATCCnnnnn_n' Cuts at: 0 603 1503 1800 Size: 603 900 297 Fragments arranged by size: 900 603 297 BfrBI ATG'CAT Cuts at: 0 460 1800 Size: 460 1340 BlpI GC'TnA_GC Cuts at: 0 1037 1800 Size: 1037 763 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 265 788 1800 Size: 265 523 1012 Fragments arranged by size: 1012 523 265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 1800 Size: 142 1658 Bpu10I CC'TnA_GC Cuts at: 0 504 1800 Size: 504 1296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 1800 Size: 634 1166 BsaAI yAC'GTr Cuts at: 0 1578 1800 Size: 1578 222 BsaHI Gr'CG_yC Cuts at: 0 1194 1800 Size: 1194 606 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 734 764 1800 Size: 734 30 1036 Fragments arranged by size: 1036 734 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 493 691 1800 Size: 493 198 1109 Fragments arranged by size: 1109 493 198 BseYI C'CCAG_C Cuts at: 0 57 596 1800 Size: 57 539 1204 Fragments arranged by size: 1204 539 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1475 1800 Size: 1475 325 BsiHKAI G_wGCw'C Cuts at: 0 251 752 1080 1800 Size: 251 501 328 720 Fragments arranged by size: 720 501 328 251 Bsp1286I G_dGCh'C Cuts at: 0 251 752 1080 1800 Size: 251 501 328 720 Fragments arranged by size: 720 501 328 251 BspHI T'CATG_A Cuts at: 0 108 1800 Size: 108 1692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 908 1800 Size: 908 892 BsrDI GCAATG_nn' Cuts at: 0 1736 1800 Size: 1736 64 BsrFI r'CCGG_y Cuts at: 0 1519 1800 Size: 1519 281 BsrGI T'GTAC_A Cuts at: 0 405 1800 Size: 405 1395 BssSI C'ACGA_G Cuts at: 0 745 1800 Size: 745 1055 BstAPI GCAn_nnn'nTGC Cuts at: 0 1075 1800 Size: 1075 725 BstXI CCAn_nnnn'nTGG Cuts at: 0 474 880 1800 Size: 474 406 920 Fragments arranged by size: 920 474 406 BstYI r'GATC_y Cuts at: 0 36 1800 Size: 36 1764 BtsI GCAGTG_nn' Cuts at: 0 1349 1800 Size: 1349 451 ClaI AT'CG_AT Cuts at: 0 1296 1800 Size: 1296 504 DraI TTT'AAA Cuts at: 0 1794 1800 Size: 1794 6 DraIII CAC_nnn'GTG Cuts at: 0 998 1800 Size: 998 802 EarI CTCTTCn'nnn_ Cuts at: 0 132 553 1800 Size: 132 421 1247 Fragments arranged by size: 1247 421 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1664 1800 Size: 1664 136 EcoICRI GAG'CTC Cuts at: 0 750 1800 Size: 750 1050 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 113 142 255 362 1325 1800 Size: 113 29 113 107 963 475 Fragments arranged by size: 963 475 113 113 107 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 1013 1800 Size: 1013 787 EcoO109I rG'GnC_Cy Cuts at: 0 1384 1800 Size: 1384 416 EcoRI G'AATT_C Cuts at: 0 828 1800 Size: 828 972 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 628 649 1800 Size: 628 21 1151 Fragments arranged by size: 1151 628 21 HaeII r_GCGC'y Cuts at: 0 519 1800 Size: 519 1281 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 577 609 1800 Size: 79 32 466 32 1191 Fragments arranged by size: 1191 466 79 32 32 HindIII A'AGCT_T Cuts at: 0 1039 1800 Size: 1039 761 KpnI G_GTAC'C Cuts at: 0 873 1800 Size: 873 927 MfeI C'AATT_G Cuts at: 0 817 1466 1800 Size: 817 649 334 Fragments arranged by size: 817 649 334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 933 951 1800 Size: 933 18 849 Fragments arranged by size: 933 849 18 MslI CAynn'nnrTG Cuts at: 0 211 1757 1800 Size: 211 1546 43 Fragments arranged by size: 1546 211 43 MspA1I CmG'CkG Cuts at: 0 1489 1800 Size: 1489 311 NsiI A_TGCA'T Cuts at: 0 462 1800 Size: 462 1338 NspI r_CATG'y Cuts at: 0 464 763 1800 Size: 464 299 1037 Fragments arranged by size: 1037 464 299 PciI A'CATG_T Cuts at: 0 759 1800 Size: 759 1041 PflMI CCAn_nnn'nTGG Cuts at: 0 1442 1800 Size: 1442 358 PpuMI rG'GwC_Cy Cuts at: 0 1384 1800 Size: 1384 416 PsiI TTA'TAA Cuts at: 0 1139 1800 Size: 1139 661 PstI C_TGCA'G Cuts at: 0 1344 1800 Size: 1344 456 PvuII CAG'CTG Cuts at: 0 1489 1800 Size: 1489 311 SacI G_AGCT'C Cuts at: 0 752 1800 Size: 752 1048 SfcI C'TryA_G Cuts at: 0 258 395 1340 1800 Size: 258 137 945 460 Fragments arranged by size: 945 460 258 137 SmlI C'TyrA_G Cuts at: 0 613 1318 1800 Size: 613 705 482 Fragments arranged by size: 705 613 482 SspI AAT'ATT Cuts at: 0 11 1155 1800 Size: 11 1144 645 Fragments arranged by size: 1144 645 11 StyI C'CwwG_G Cuts at: 0 901 1215 1532 1735 1800 Size: 901 314 317 203 65 Fragments arranged by size: 901 317 314 203 65 TatI w'GTAC_w Cuts at: 0 405 1800 Size: 405 1395 XmnI GAAnn'nnTTC Cuts at: 0 608 1588 1800 Size: 608 980 212 Fragments arranged by size: 980 608 212 Enzymes that do cut: Acc65I AcuI AfeI AflII AflIII ApoI BaeI BanI BanII BbsI BciVI BfrBI BlpI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtsI ClaI DraI DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KpnI MfeI MmeI MslI MspA1I NsiI NspI PciI PflMI PpuMI PsiI PstI PvuII SacI SfcI SmlI SspI StyI TatI XmnI Enzymes that do not cut: AarI AatII AccI AclI AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BmtI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI DrdI EaeI EagI EcoRV FseI FspI FspAI HincII HpaI KasI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI