################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HpaII HphI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AciI 486 GCGG CCGC(-3/-1) AflIII 368 ACATGT A^C[GA][CT]GT AlwNI 11 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 507 GAGCAC G[AT]GC[AT]^C AvaII 244 GGACC G^G[AT]CC BanI 387 GGCACC G^G[CT][GA]CC BbsI 547 GTCTTC GAAGAC(2/6) BciVI 612 GGATAC GTATCC(6/5) BmyI 507 GAGCAC G[AGT]GC[ACT]^C BpuAI 547 GTCTTC GAAGAC(2/6) BsaJI 320 CCTTGG C^C[ACGT][ACGT]GG BseRI 377 GAGGAG GAGGAG(10/8) BsgI 140 CTGCAC GTGCAG(16/14) BsiHKAI 507 GAGCAC G[AT]GC[AT]^C BsmAI 536 GAGAC GTCTC(1/5) BsmFI 243 GGGAC GGGAC(10/14) Bsp1286I 507 GAGCAC G[AGT]GC[ACT]^C BspLU11I 368 ACATGT A^CATGT BspMI 10 GCAGGT ACCTGC(4/8) BsrDI 492 CATTGC GCAATG(2/0) BsrI 542 ACTGG ACTGG(1/-1) BssKI 258 CCTGG ^CC[ACGT]GG BstAPI 10 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 258 CCTGG CC^[AT]GG BstXI 233 CCAACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 176 GGATCT [GA]^GATC[CT] Cac8I 187 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 244 GGACC G^G[ACGT]CC DdeI 194 CTGAG C^T[ACGT]AG DraI 211 TTTAAA TTT^AAA DraII 243 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 538 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 302 GAAGAG CTCTTC(1/4) EcoO109I 243 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 258 CCTGG ^CC[AT]GG EcoT14I 320 CCTTGG C^C[AT][AT]GG EcoT22I 660 ATGCAT ATGCA^T HindIII 185 AAGCTT A^AGCTT Ksp632I 302 GAAGAG CTCTTC(1/4) MflI 176 GGATCT [GA]^GATC[CT] MspA1I 137 CAGCTG C[AC]G^C[GT]G MvaI 258 CCTGG CC^[AT]GG NlaIII 369 CATG CATG^ NsiI 660 ATGCAT ATGCA^T NspI 368 ACATGT [GA]CATG^[CT] PpuMI 243 GGGACCC [GA]G^G[AT]CC[CT] PvuII 137 CAGCTG CAG^CTG Sau96I 244 GGACC G^G[ACGT]CC ScrFI 258 CCTGG CC^[ACGT]GG SmlI 71 CTCAAG C^T[CT][GA]AG SspI 359 AATATT AAT^ATT StyI 320 CCTTGG C^C[AT][AT]GG TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 248 CCAAAATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 176 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### AlwI 176 GGATC GGATC(4/5) 308 GGATC HaeIII 411 GGCC GG^CC 635 GGCC MaeII 144 ACGT A^CGT 236 ACGT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI BstF5I DpnII Fnu4HI FokI ItaI MboI MboII MnlI MseI MwoI NdeII NlaIV Sau3AI SfaNI Tru9I TseI