(Circular) (Six-base) MAPSORT of: pFN21AE3070.seq Check: 2636 from: 1 to: 5386 pFN21AE3070 5386 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2006 2006 Size: 5386 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3196 278 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 1937 Size: 792 4594 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2664 3449 4833 5101 1632 Size: 800 232 785 1384 268 1917 Fragments arranged by size: 1917 1384 800 785 268 232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 2373 4285 5262 1203 Size: 1170 1912 977 1327 Fragments arranged by size: 1912 1327 1170 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5386 AlwNI CAG_nnn'CTG Cuts at: 2021 3129 4701 2021 Size: 1108 1572 2706 Fragments arranged by size: 2706 1572 1108 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2695 2921 3073 5182 1493 Size: 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 3940 160 Size: 872 2908 1606 Fragments arranged by size: 2908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2711 1870 Size: 73 768 4545 Fragments arranged by size: 4545 768 73 BamHI G'GATC_C Cuts at: 2716 3083 2716 Size: 367 5019 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 2707 4162 618 Size: 325 217 627 605 315 1455 1842 Fragments arranged by size: 1842 1455 627 605 325 315 217 BanII G_rGCy'C Cuts at: 1391 2705 1391 Size: 1314 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2545 928 Size: 971 646 3769 Fragments arranged by size: 3769 971 646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3577 3611 1903 Size: 34 1640 34 3678 Fragments arranged by size: 3678 1640 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2611 3278 4488 2611 Size: 667 1210 3509 Fragments arranged by size: 3509 1210 667 BclI T'GATC_A Cuts at: 1548 1548 Size: 5386 BfrBI ATG'CAT Cuts at: 2667 2667 Size: 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3997 136 Size: 107 122 71 631 605 2325 1525 Fragments arranged by size: 2325 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 3447 4603 1790 Size: 1657 1156 2573 Fragments arranged by size: 2573 1657 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5386 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2557 3117 4075 Size: 416 53 83 399 24 1140 560 958 Cuts at: 4075 442 Size: 1753 Fragments arranged by size: 1753 1140 958 560 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4046 1239 Size: 2807 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2061 3511 4376 4674 2061 Size: 1450 865 298 2773 Fragments arranged by size: 2773 1450 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4049 882 Size: 617 2550 2219 Fragments arranged by size: 2550 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3177 493 Size: 711 1973 2702 Fragments arranged by size: 2702 1973 711 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3193 3575 4210 275 Size: 53 83 186 936 1660 382 635 1451 Fragments arranged by size: 1660 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3819 4217 4491 4638 5049 3819 Size: 398 274 147 411 4156 Fragments arranged by size: 4156 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 2373 2403 1178 Size: 30 165 30 743 30 197 30 4161 Fragments arranged by size: 4161 743 197 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2397 1186 Size: 1211 4175 BseYI C'CCAG_C Cuts at: 4589 5085 4589 Size: 496 4890 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2130 1163 Size: 573 394 4419 Fragments arranged by size: 4419 573 394 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 3597 4625 1540 Size: 275 186 835 761 1028 2301 Fragments arranged by size: 2301 1028 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2516 3447 3532 4603 2516 Size: 931 85 1071 3299 Fragments arranged by size: 3299 1071 931 85 BsmI GAATG_Cn' Cuts at: 2897 2990 2897 Size: 93 5293 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2516 2705 3447 3532 4603 1391 Size: 399 726 189 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 726 399 189 85 BspEI T'CCGG_A Cuts at: 5049 5049 Size: 5386 BspHI T'CATG_A Cuts at: 3275 5005 3275 Size: 1730 3656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2006 2741 844 Size: 1162 735 3489 Fragments arranged by size: 3489 1162 735 BsrBI CCG'CTC Cuts at: 1394 1823 3273 1394 Size: 429 1450 3507 Fragments arranged by size: 3507 1450 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2496 3881 4055 65 Size: 1183 1248 1385 174 1396 Fragments arranged by size: 1396 1385 1248 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4030 4217 1700 Size: 110 2220 187 2869 Fragments arranged by size: 2869 2220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 2021 794 Size: 1227 4159 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2245 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2181 2716 3083 3468 3485 4937 5023 5035 Size: 209 535 367 385 17 1452 86 12 Cuts at: 5035 5381 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 535 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4834 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4647 Fragments arranged by size: 4647 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2902 3696 3716 2052 Size: 850 794 20 3722 Fragments arranged by size: 3722 850 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2013 2048 529 Size: 35 1449 35 3867 Fragments arranged by size: 3867 1449 35 35 DraI TTT'AAA Cuts at: 2218 2690 2218 Size: 472 4914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2549 4393 809 Size: 507 1233 1844 1802 Fragments arranged by size: 1844 1802 1233 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2833 3722 5351 Size: 54 1425 50 275 18 1003 889 1629 Cuts at: 5351 8 Size: 43 Fragments arranged by size: 1629 1425 1003 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 1537 Size: 275 1021 4090 Fragments arranged by size: 4090 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2302 3316 1353 Size: 949 1014 3423 Fragments arranged by size: 3423 1014 949 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3973 4347 4493 387 Size: 1064 138 2384 374 146 1280 Fragments arranged by size: 2384 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2664 3449 4046 4833 5101 1239 Size: 393 800 232 785 597 787 268 1524 Fragments arranged by size: 1524 800 787 785 597 393 268 232 EcoO109I rG'GnC_Cy Cuts at: 2249 2249 Size: 5386 EcoRI G'AATT_C Cuts at: 2695 3073 2695 Size: 378 5008 FspI TGC'GCA Cuts at: 3892 3892 Size: 5386 HaeII r_GCGC'y Cuts at: 1678 4533 1678 Size: 2855 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 4027 4059 4101 Size: 32 135 32 669 32 1754 32 42 Cuts at: 4101 4133 1373 Size: 32 2626 Fragments arranged by size: 2626 1754 669 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2730 2976 669 Size: 988 281 792 246 3079 Fragments arranged by size: 3079 988 792 281 246 HindIII A'AGCT_T Cuts at: 748 2190 748 Size: 1442 3944 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2284 4500 4684 1305 Size: 979 2216 184 2007 Fragments arranged by size: 2216 2007 979 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3564 Fragments arranged by size: 3564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3344 3703 3862 518 Size: 648 2178 359 159 2042 Fragments arranged by size: 2178 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2802 3479 4627 4872 1606 Size: 538 658 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 658 538 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4834 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NsiI A_TGCA'T Cuts at: 2669 2669 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2377 2744 4289 1132 Size: 97 1148 367 1545 2229 Fragments arranged by size: 2229 1545 1148 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5386 PciI A'CATG_T Cuts at: 2373 4285 2373 Size: 1912 3474 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5386 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 4968 5000 3455 Size: 32 1481 32 3841 Fragments arranged by size: 3841 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2249 2249 Size: 5386 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PstI C_TGCA'G Cuts at: 830 2738 830 Size: 1908 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 PvuII CAG'CTG Cuts at: 2144 2144 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2728 1936 Size: 792 4594 SanDI GG'GwC_CC Cuts at: 2249 2249 Size: 5386 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SfcI C'TryA_G Cuts at: 826 1045 2734 3869 4550 4741 826 Size: 219 1689 1135 681 191 1471 Fragments arranged by size: 1689 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2713 1872 Size: 841 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2076 3490 4391 4653 820 Size: 197 926 133 1414 901 262 1553 Fragments arranged by size: 1553 1414 926 901 262 197 133 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2744 1132 Size: 1612 3774 SspI AAT'ATT Cuts at: 5 52 2366 3310 5 Size: 47 2314 944 2081 Fragments arranged by size: 2314 2081 944 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2325 2825 513 Size: 552 1260 500 3074 Fragments arranged by size: 3074 1260 552 500 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3413 3430 3583 3768 4213 325 Size: 1255 1833 17 153 185 445 1498 Fragments arranged by size: 1833 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3632 5344 96 Size: 274 80 33 51 1443 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2373 2405 5346 5378 2373 Size: 32 2941 32 2381 Fragments arranged by size: 2941 2381 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2260 Size: 5386 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5386 XmaI C'CCGG_G Cuts at: 1870 2711 1870 Size: 841 4545 XmnI GAAnn'nnTTC Cuts at: 3515 3515 Size: 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3194 276 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI