Restriction map for
pFN21AE3146
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HphI
KasI KpnI KspI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 576 GGATC GGATC(4/5)
AlwNI 689 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 307 GGTCC G^G[AT]CC
BanII 812 GGGCTC G[GA]GC[CT]^C
BbvCI 889 CCTCAGC CCTCAGC(-5/-2)
BbvI 1020 GCTGC GCAGC(8/12)
BfaI 898 CTAG C^TAG
BmyI 812 GGGCTC G[AGT]GC[ACT]^C
BsaAI 862 TACGTA [CT]AC^GT[GA]
BsaWI 271 ACCGGA [AT]^CCGG[AT]
BsgI 505 GTGCAG GTGCAG(16/14)
BsmFI 699 GGGAC GGGAC(10/14)
Bsp1286I 812 GGGCTC G[AGT]GC[ACT]^C
BspHI 1013 TCATGA T^CATGA
BspMI 426 ACCTGC ACCTGC(4/8)
BsrDI 967 GCAATG GCAATG(2/0)
Cfr13I 307 GGTCC G^G[ACGT]CC
DraII 306 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 306 AGGTCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 1020 GCTGC GC^[ACGT]GC
HaeIII 792 GGCC GG^CC
HapII 272 CCGG C^CGG
HgaI 755 GACGC GACGC(5/10)
HincII 582 GTTAAC GT[CT]^[GA]AC
HindII 582 GTTAAC GT[CT]^[GA]AC
HindIII 1043 AAGCTT A^AGCTT
HpaI 582 GTTAAC GTT^AAC
HpaII 272 CCGG C^CGG
ItaI 1020 GCTGC GC^[ACGT]GC
MaeI 898 CTAG C^TAG
MaeII 863 ACGT A^CGT
MspI 272 CCGG C^CGG
PleI 701 GACTC GAGTC(4/5)
PpuMI 306 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1013 TCATGA T^CATGA
Sau96I 307 GGTCC G^G[ACGT]CC
SfaNI 786 GATGC GCATC(5/9)
SnaBI 862 TACGTA TAC^GTA
SspI 534 AATATT AAT^ATT
TseI 1020 GCTGC G^C[AT]GC
TspRI 903 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 897 TCTAGA T^CTAGA
XcmI 362 CCAAGGAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 74 GTAC GT^AC
284 GTAC
BsmAI 140 GAGAC GTCTC(1/5)
1064 GAGAC
BsrI 372 ACTGG ACTGG(1/-1)
601 ACTGG
BstF5I 180 CATCC GGATG(2/0)
912 GGATG
Cac8I 90 GCAAGC GC[ACGT]^[ACGT]GC
789 GCAGGC
DraI 51 TTTAAA TTT^AAA
1038 TTTAAA
EcoT14I 362 CCAAGG C^C[AT][AT]GG
794 CCAAGG
FokI 180 CATCC GGATG(9/13)
912 GGATG
MslI 340 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
472 CATCAGTATG
RsaI 74 GTAC GT^AC
284 GTAC
StyI 362 CCAAGG C^C[AT][AT]GG
794 CCAAGG
TaqI 483 TCGA T^CGA
753 TCGA
TthHB8I 483 TCGA T^CGA
753 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsaJI
BseRI BssKI BstNI DdeI DpnII
EarI EcoRII HinfI Ksp632I MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfcI TfiI Tru9I Tsp509I