(Circular) (Six-base) MAPSORT of: pFN21AE3146.seq Check: 8960 from: 1 to: 5776 pFN21AE3146 5776 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2235 3839 5223 5491 1632 Size: 454 149 1604 1384 268 1917 Fragments arranged by size: 1917 1604 1384 454 268 149 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2699 3519 5091 2699 Size: 820 1572 3384 Fragments arranged by size: 3384 1572 820 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2414 2490 2773 2998 3085 3311 3463 5572 Size: 921 76 283 225 87 226 152 2109 Cuts at: 5572 1493 Size: 1697 Fragments arranged by size: 2109 1697 921 283 226 225 152 87 76 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2821 3095 1391 Size: 1430 274 4072 Fragments arranged by size: 4072 1430 274 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BbvCI CC'TCA_GC Cuts at: 2895 2895 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 3837 4993 1790 Size: 2047 1156 2573 Fragments arranged by size: 2573 2047 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2399 2441 2526 4436 1239 Size: 1160 42 85 1910 2579 Fragments arranged by size: 2579 1910 1160 85 42 Bpu10I CC'TnA_GC Cuts at: 2895 2895 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2869 3567 493 Size: 711 1665 698 2702 Fragments arranged by size: 2702 1665 711 698 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2276 4209 4607 4881 5028 5439 2276 Size: 1933 398 274 147 411 2613 Fragments arranged by size: 2613 1933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2001 2031 2493 2523 1178 Size: 30 165 30 598 30 462 30 4431 Fragments arranged by size: 4431 598 462 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 2963 2999 1186 Size: 839 938 36 3963 Fragments arranged by size: 3963 938 839 36 BseYI C'CCAG_C Cuts at: 2024 4979 5475 2024 Size: 2955 496 2325 Fragments arranged by size: 2955 2325 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2531 1163 Size: 573 795 4408 Fragments arranged by size: 4408 795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2821 3095 3837 3922 4993 1391 Size: 399 1031 274 742 85 1071 2174 Fragments arranged by size: 2174 1071 1031 742 399 274 85 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3018 3665 5395 3018 Size: 647 1730 3399 Fragments arranged by size: 3399 1730 647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 3131 844 Size: 1596 691 3489 Fragments arranged by size: 3489 1596 691 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2979 4271 4445 65 Size: 1183 1731 1292 174 1396 Fragments arranged by size: 1731 1396 1292 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 2058 3045 3080 2058 Size: 987 35 4754 Fragments arranged by size: 4754 987 35 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3223 4112 5741 Size: 54 1425 50 275 18 1393 889 1629 Cuts at: 5741 8 Size: 43 Fragments arranged by size: 1629 1425 1393 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2907 2925 2940 3706 1353 Size: 1554 18 15 766 3423 Fragments arranged by size: 3423 1554 766 18 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2235 2399 2441 2526 3839 4436 Size: 393 454 149 164 42 85 1313 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1313 787 597 454 393 268 164 149 85 42 EcoO109I rG'GnC_Cy Cuts at: 2312 2312 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3000 3032 4417 4449 4491 Size: 32 135 32 1428 32 1385 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1385 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2589 3120 3366 669 Size: 988 281 651 531 246 3079 Fragments arranged by size: 3079 988 651 531 281 246 HindIII A'AGCT_T Cuts at: 748 3048 748 Size: 2300 3476 HpaI GTT'AAC Cuts at: 2589 3366 2589 Size: 777 4999 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2533 4890 5074 1305 Size: 1228 2357 184 2007 Fragments arranged by size: 2357 2007 1228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 2481 3734 4093 4252 518 Size: 648 1183 132 1253 359 159 2042 Fragments arranged by size: 2042 1253 1183 648 359 159 132 MspA1I CmG'CkG Cuts at: 1606 3192 3869 5017 5262 1606 Size: 1586 677 1148 245 2120 Fragments arranged by size: 2120 1586 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2312 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2140 2574 2732 3124 4259 4940 5131 Size: 219 1095 434 158 392 1135 681 191 Cuts at: 5131 826 Size: 1471 Fragments arranged by size: 1471 1135 1095 681 434 392 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2869 3567 493 Size: 2376 698 2702 Fragments arranged by size: 2702 2376 698 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 2541 3700 5 Size: 47 2489 1159 2081 Fragments arranged by size: 2489 2081 1159 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 2799 3215 513 Size: 552 939 363 432 416 3074 Fragments arranged by size: 3074 939 552 432 416 363 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2839 3803 3820 3973 4158 4603 325 Size: 1255 1259 964 17 153 185 445 1498 Fragments arranged by size: 1498 1259 1255 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2288 4022 5734 Size: 274 80 33 51 1443 311 1734 1712 Cuts at: 5734 96 Size: 138 Fragments arranged by size: 1734 1712 1443 311 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2493 2525 5736 5768 2001 Size: 32 460 32 3211 32 2009 Fragments arranged by size: 3211 2009 460 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 2902 3112 2902 Size: 210 5566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 2374 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI