################################## # NO CUT ENZYMES ( 21 enzymes) # ################################## AatII AclI AscI BsaBI BspDI BssHII Bst1107I BstZ17I ClaI MamI MluI NotI NruI PacI PmeI Psp1406I PvuI SalI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 10739 TCCGGA T^CCGGA AflII 12636 CTTAAG C^TTAAG AgeI 3570 ACCGGT A^CCGGT AhdI 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 6073 ATTAAT AT^TAAT AsnI 6073 ATTAAT AT^TAAT Asp700I 8752 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BfrI 12636 CTTAAG C^TTAAG BglII 3620 AGATCT A^GATCT BseAI 10739 TCCGGA T^CCGGA BsiWI 5749 CGTACG C^GTACG BspEI 10739 TCCGGA T^CCGGA BstEII 4509 GGTGACC G^GT[ACGT]ACC BstPI 4509 GGTGACC G^GT[ACGT]ACC CpoI 2209 CGGTCCG CG^G[AT]CCG DraI 11928 TTTAAA TTT^AAA DrdI 4108 GACAAATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2902 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4509 GGTGACC G^GT[ACGT]ACC EcoRI 6670 GAATTC G^AATTC FseI 5380 GGCCGGCC GGCCGG^CC HpaI 3613 GTTAAC GTT^AAC KspI 7493 CCGCGG CCGC^GG MfeI 10180 CAATTG C^AATTG MroI 10739 TCCGGA T^CCGGA MunI 10180 CAATTG C^AATTG PinAI 3570 ACCGGT A^CCGGT PshAI 15079 GACATGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6073 ATTAAT AT^TAAT RsrII 2209 CGGTCCG CG^G[AT]CCG SacII 7493 CCGCGG CCGC^GG SbfI 833 CCTGCAGG CCTGCA^GG ScaI 4745 AGTACT AGT^ACT SnaBI 4537 TACGTA TAC^GTA Sse8387I 833 CCTGCAGG CCTGCA^GG XbaI 14016 TCTAGA T^CTAGA XmnI 8752 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AviII 6615 TGCGCA TGC^GCA 16169 TGCGCA BbrPI 1198 CACGTG CAC^GTG 3563 CACGTG BclI 10499 TGATCA T^GATCA 15992 TGATCA Bsp1407I 8646 TGTACA T^GTACA 10596 TGTACA BsrGI 8646 TGTACA T^GTACA 10596 TGTACA BssSI 3211 CACGAG CACGAG(-5/-1) 3941 CTCGTG BstBI 4756 TTCGAA TT^CGAA 11114 TTCGAA EagI 1459 CGGCCG C^GGCCG 15709 CGGCCG EclXI 1459 CGGCCG C^GGCCG 15709 CGGCCG Eco52I 1459 CGGCCG C^GGCCG 15709 CGGCCG EcoRV 9187 GATATC GAT^ATC 10252 GATATC EcoT22I 4585 ATGCAT ATGCA^T 15400 ATGCAT FbaI 10499 TGATCA T^GATCA 15992 TGATCA FspI 6615 TGCGCA TGC^GCA 16169 TGCGCA NdeI 7127 CATATG CA^TATG 10060 CATATG NheI 10368 GCTAGC G^CTAGC 15493 GCTAGC NsiI 4585 ATGCAT ATGCA^T 15400 ATGCAT NspV 4756 TTCGAA TT^CGAA 11114 TTCGAA PaeR7I 5506 CTCGAG C^TCGAG 9640 CTCGAG PmaCI 1198 CACGTG CAC^GTG 3563 CACGTG PmlI 1198 CACGTG CAC^GTG 3563 CACGTG SfuI 4756 TTCGAA TT^CGAA 11114 TTCGAA SgrAI 3569 CACCGGTG C[GA]^CCGG[CT]G 6431 CGCCGGCG SspBI 8646 TGTACA T^GTACA 10596 TGTACA XhoI 5506 CTCGAG C^TCGAG 9640 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 174 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI Asp718I AspHI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI