Restriction map for
pFN21AE9397
(Linear) (Six-base) MAPSORT of: KIEE9397.seq Check: 6487 from: 1 to: 16608
KIEE9397.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 2124 7495 7860 9438 9587 9940 12319
Size: 384 1740 5371 365 1578 149 353 2379
Cuts at: 12319 12401 13083 16608
Size: 82 682 3525
Fragments arranged by size:
5371 3525 2379 1740 1578 682 384 365
353 149 82
AccI GT'mk_AC
Cuts at: 0 225 3503 6403 12083 15332 15461 16608
Size: 225 3278 2900 5680 3249 129 1147
Fragments arranged by size:
5680 3278 3249 2900 1147 225 129
Acc65I G'GTAC_C
Cuts at: 0 7984 10098 13179 13507 15069 16608
Size: 7984 2114 3081 328 1562 1539
Fragments arranged by size:
7984 3081 2114 1562 1539 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1640 1721 1775 1937 3067 5255 5989
Size: 136 1504 81 54 162 1130 2188 734
Cuts at: 5989 6445 7313 7442 8771 10591 11066 11608 11680
Size: 456 868 129 1329 1820 475 542 72
Cuts at: 11680 11716 11758 12083 13952 13985 14587 14623 14750
Size: 36 42 325 1869 33 602 36 127
Cuts at: 14750 14921 15880 16608
Size: 171 959 728
Fragments arranged by size:
2188 1869 1820 1504 1329 1130 959 868
734 728 602 542 475 456 325 171
162 136 129 127 81 72 54 42
36 36 33
AfeI AGC'GCT
Cuts at: 0 511 6010 11667 16608
Size: 511 5499 5657 4941
Fragments arranged by size:
5657 5499 4941 511
AflII C'TTAA_G
Cuts at: 0 12636 16608
Size: 12636 3972
AflIII A'CryG_T
Cuts at: 0 108 877 4130 5294 15524 16190 16349 16371
Size: 108 769 3253 1164 10230 666 159 22
Cuts at: 16371 16608
Size: 237
Fragments arranged by size:
10230 3253 1164 769 666 237 159 108
22
AgeI A'CCGG_T
Cuts at: 0 3570 16608
Size: 3570 13038
AhdI GACnn_n'nnGTC
Cuts at: 0 2907 16608
Size: 2907 13701
AleI CACnn'nnGTG
Cuts at: 0 3813 4240 6944 15199 16608
Size: 3813 427 2704 8255 1409
Fragments arranged by size:
8255 3813 2704 1409 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4807 8110 10420 10655 14594 15070 15331 16608
Size: 4807 3303 2310 235 3939 476 261 1277
Fragments arranged by size:
4807 3939 3303 2310 1277 476 261 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5281 5313 10522 10554 16608
Size: 5281 32 5209 32 6054
Fragments arranged by size:
6054 5281 5209 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 15 67 1129 3241 3409 3799 4780 5533
Size: 15 52 1062 2112 168 390 981 753
Cuts at: 5533 5715 5912 6160 7945 8125 8587 8602 9682
Size: 182 197 248 1785 180 462 15 1080
Cuts at: 9682 9868 9981 10200 10672 11196 11364 11634 11712
Size: 186 113 219 472 524 168 270 78
Cuts at: 11712 12526 12549 13369 13671 13742 14362 14479 14551
Size: 814 23 820 302 71 620 117 72
Cuts at: 14551 15305 15570 15744 16221 16608
Size: 754 265 174 477 387
Fragments arranged by size:
2112 1785 1080 1062 981 820 814 754
753 620 524 477 472 462 390 387
302 270 265 248 219 197 186 182
180 174 168 168 117 113 78 72
71 52 23 15 15
ApaI G_GGCC'C
Cuts at: 0 314 463 1232 3981 4496 5002 6612 6899
Size: 314 149 769 2749 515 506 1610 287
Cuts at: 6899 7700 7784 8695 9283 11390 11407 11455 11464
Size: 801 84 911 588 2107 17 48 9
Cuts at: 11464 12119 12460 12853 14205 14277 15419 16608
Size: 655 341 393 1352 72 1142 1189
Fragments arranged by size:
2749 2107 1610 1352 1189 1142 911 801
769 655 588 515 506 393 341 314
287 149 84 72 48 17 9
ApaLI G'TGCA_C
Cuts at: 0 3566 15169 16138 16608
Size: 3566 11603 969 470
Fragments arranged by size:
11603 3566 969 470
ApoI r'AATT_y
Cuts at: 0 4111 6670 8564 10237 16608
Size: 4111 2559 1894 1673 6371
Fragments arranged by size:
6371 4111 2559 1894 1673
AseI AT'TA_AT
Cuts at: 0 6074 16608
Size: 6074 10534
AvaI C'yCGr_G
Cuts at: 0 2734 3469 3752 5506 6995 7023 7223 8968
Size: 2734 735 283 1754 1489 28 200 1745
Cuts at: 8968 9640 10383 11492 13253 13970 14231 14468 15922
Size: 672 743 1109 1761 717 261 237 1454
Cuts at: 15922 16608
Size: 686
Fragments arranged by size:
2734 1761 1754 1745 1489 1454 1109 743
735 717 686 672 283 261 237 200
28
AvrII C'CTAG_G
Cuts at: 0 384 3351 8544 16608
Size: 384 2967 5193 8064
Fragments arranged by size:
8064 5193 2967 384
BamHI G'GATC_C
Cuts at: 0 7864 8964 9613 11065 13128 16608
Size: 7864 1100 649 1452 2063 3480
Fragments arranged by size:
7864 3480 2063 1452 1100 649
BanI G'GyrC_C
Cuts at: 0 535 2884 4689 4713 4829 5212 5587 5929
Size: 535 2349 1805 24 116 383 375 342
Cuts at: 5929 6444 6495 6570 6634 6691 6934 7071 7582
Size: 515 51 75 64 57 243 137 511
Cuts at: 7582 7984 9073 9952 10098 10144 10335 13179 13215
Size: 402 1089 879 146 46 191 2844 36
Cuts at: 13215 13320 13507 14760 15069 15292 15962 16279 16608
Size: 105 187 1253 309 223 670 317 329
Fragments arranged by size:
2844 2349 1805 1253 1089 879 670 535
515 511 402 383 375 342 329 317
309 243 223 191 187 146 137 116
105 75 64 57 51 46 36 24
BanII G_rGCy'C
Cuts at: 0 221 307 314 414 463 988 1154 1232
Size: 221 86 7 100 49 525 166 78
Cuts at: 1232 1283 3056 3538 3674 3685 3766 3954 3981
Size: 51 1773 482 136 11 81 188 27
Cuts at: 3981 4496 4742 4889 5002 5698 5903 5923 6442
Size: 515 246 147 113 696 205 20 519
Cuts at: 6442 6473 6557 6612 6778 6809 6899 7016 7024
Size: 31 84 55 166 31 90 117 8
Cuts at: 7024 7514 7700 7784 8168 8695 9283 9425 9641
Size: 490 186 84 384 527 588 142 216
Cuts at: 9641 9712 9901 10114 11390 11407 11455 11464 11683
Size: 71 189 213 1276 17 48 9 219
Cuts at: 11683 12039 12119 12460 12853 13281 13600 14176 14205
Size: 356 80 341 393 428 319 576 29
Cuts at: 14205 14277 14464 14545 14647 14792 15419 15439 15875
Size: 72 187 81 102 145 627 20 436
Cuts at: 15875 16229 16608
Size: 354 379
Fragments arranged by size:
1773 1276 696 627 588 576 527 525
519 515 490 482 436 428 393 384
379 356 354 341 319 246 221 219
216 213 205 189 188 187 186 166
166 147 145 142 136 117 113 102
100 90 86 84 84 81 81 80
78 72 71 55 51 49 48 31
31 29 27 20 20 17 11 9
8 7
BbeI G_GCGC'C
Cuts at: 0 4693 4833 5216 6638 15966 16608
Size: 4693 140 383 1422 9328 642
Fragments arranged by size:
9328 4693 1422 642 383 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 3365 3936 5995 6966 7940 8773 8824 10951
Size: 3365 571 2059 971 974 833 51 2127
Cuts at: 10951 14241 15441 15750 16608
Size: 3290 1200 309 858
Fragments arranged by size:
3365 3290 2127 2059 1200 974 971 858
833 571 309 51
BbvCI CC'TCA_GC
Cuts at: 0 2294 2375 2396 2780 3851 7652 8799 11957
Size: 2294 81 21 384 1071 3801 1147 3158
Cuts at: 11957 12248 12644 13768 14501 16608
Size: 291 396 1124 733 2107
Fragments arranged by size:
3801 3158 2294 2107 1147 1124 1071 733
396 384 291 81 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 513 547 1138 1172 8176 8210 8971 9005
Size: 513 34 591 34 7004 34 761 34
Cuts at: 9005 13234 13268 16608
Size: 4229 34 3340
Fragments arranged by size:
7004 4229 3340 761 591 513 34 34
34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 883 9483 13989 16222 16608
Size: 883 8600 4506 2233 386
Fragments arranged by size:
8600 4506 2233 883 386
BclI T'GATC_A
Cuts at: 0 10499 15992 16608
Size: 10499 5493 616
Fragments arranged by size:
10499 5493 616
BfrBI ATG'CAT
Cuts at: 0 4587 15402 16608
Size: 4587 10815 1206
Fragments arranged by size:
10815 4587 1206
BglI GCCn_nnn'nGGC
Cuts at: 0 979 2845 4859 5510 5741 7435 7497 8809
Size: 979 1866 2014 651 231 1694 62 1312
Cuts at: 8809 8998 11345 11416 11459 12767 13642 16608
Size: 189 2347 71 43 1308 875 2966
Fragments arranged by size:
2966 2347 2014 1866 1694 1312 1308 979
875 651 231 189 71 62 43
BglII A'GATC_T
Cuts at: 0 3620 16608
Size: 3620 12988
BlpI GC'TnA_GC
Cuts at: 0 987 1085 3203 5229 5608 7133 8162 11669
Size: 987 98 2118 2026 379 1525 1029 3507
Cuts at: 11669 12401 14416 14822 15433 16608
Size: 732 2015 406 611 1175
Fragments arranged by size:
3507 2118 2026 2015 1525 1175 1029 987
732 611 406 379 98
Bme1580I G_kGCm'C
Cuts at: 0 314 463 811 1199 1232 3570 3981 4496
Size: 314 149 348 388 33 2338 411 515
Cuts at: 4496 4718 5002 5567 6333 6500 6612 6899 7585
Size: 222 284 565 766 167 112 287 686
Cuts at: 7585 7700 7784 8674 8695 9283 9505 9530 9957
Size: 115 84 890 21 588 222 25 427
Cuts at: 9957 10147 11390 11407 11455 11464 12119 12389 12460
Size: 190 1243 17 48 9 655 270 71
Cuts at: 12460 12853 13272 13323 13466 13989 14205 14277 14892
Size: 393 419 51 143 523 216 72 615
Cuts at: 14892 15173 15186 15225 15419 16014 16142 16431 16608
Size: 281 13 39 194 595 128 289 177
Fragments arranged by size:
2338 1243 890 766 686 655 615 595
588 565 523 515 427 419 411 393
388 348 314 289 287 284 281 270
222 222 216 194 190 177 167 149
143 128 115 112 84 72 71 51
48 39 33 25 21 17 13 9
BmgBI CAC'GTC
Cuts at: 0 878 1373 2039 3964 16608
Size: 878 495 666 1925 12644
Fragments arranged by size:
12644 1925 878 666 495
BmrI ACTGGGnnnn_n'
Cuts at: 0 215 326 1278 2499 3016 3114 3257 4034
Size: 215 111 952 1221 517 98 143 777
Cuts at: 4034 4737 5554 5561 6343 7242 7254 8616 8668
Size: 703 817 7 782 899 12 1362 52
Cuts at: 8668 9182 9902 11481 13275 13433 13741 14239 16608
Size: 514 720 1579 1794 158 308 498 2369
Fragments arranged by size:
2369 1794 1579 1362 1221 952 899 817
782 777 720 703 517 514 498 308
215 158 143 111 98 52 12 7
BmtI G_CTAG'C
Cuts at: 0 10372 15497 16608
Size: 10372 5125 1111
Fragments arranged by size:
10372 5125 1111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1044 9561 12135 12613 15453 16608
Size: 1044 8517 2574 478 2840 1155
Fragments arranged by size:
8517 2840 2574 1155 1044 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 691 1486 1564 2179 2953 2996 3671
Size: 416 275 795 78 615 774 43 675
Cuts at: 3671 4688 5014 5308 5790 6162 6571 6973 8047
Size: 1017 326 294 482 372 409 402 1074
Cuts at: 8047 8129 9109 9997 11300 11462 12053 12517 12739
Size: 82 980 888 1303 162 591 464 222
Cuts at: 12739 12802 13510 14305 15766 16473 16608
Size: 63 708 795 1461 707 135
Fragments arranged by size:
1461 1303 1074 1017 980 888 795 795
774 708 707 675 615 591 482 464
416 409 402 372 326 294 275 222
162 135 82 78 63 43
Bpu10I CC'TnA_GC
Cuts at: 0 2294 2375 2396 2558 2780 2978 3851 5347
Size: 2294 81 21 162 222 198 873 1496
Cuts at: 5347 5922 7652 8011 8799 9161 10316 10490 11957
Size: 575 1730 359 788 362 1155 174 1467
Cuts at: 11957 12248 12644 13768 14175 14501 16608
Size: 291 396 1124 407 326 2107
Fragments arranged by size:
2294 2107 1730 1496 1467 1155 1124 873
788 575 407 396 362 359 326 291
222 198 174 162 81 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1615 2584 4237 4933 6073 6463 10672
Size: 1297 318 969 1653 696 1140 390 4209
Cuts at: 10672 12923 14643 14917 16608
Size: 2251 1720 274 1691
Fragments arranged by size:
4209 2251 1720 1691 1653 1297 1140 969
696 390 318 274
BsaI GGTCTCn'nnnn_
Cuts at: 0 126 3195 3735 5029 7448 7666 8697 9085
Size: 126 3069 540 1294 2419 218 1031 388
Cuts at: 9085 12945 13074 15202 16102 16608
Size: 3860 129 2128 900 506
Fragments arranged by size:
3860 3069 2419 2128 1294 1031 900 540
506 388 218 129 126
BsaAI yAC'GTr
Cuts at: 0 1200 3565 4539 4674 16608
Size: 1200 2365 974 135 11934
Fragments arranged by size:
11934 2365 1200 974 135
BsaHI Gr'CG_yC
Cuts at: 0 1600 3933 4690 4830 5213 6635 9375 13919
Size: 1600 2333 757 140 383 1422 2740 4544
Cuts at: 13919 15767 15963 16608
Size: 1848 196 645
Fragments arranged by size:
4544 2740 2333 1848 1600 1422 757 645
383 196 140
BsaWI w'CCGG_w
Cuts at: 0 183 1566 1779 2130 3570 7553 10739 16608
Size: 183 1383 213 351 1440 3983 3186 5869
Fragments arranged by size:
5869 3983 3186 1440 1383 351 213 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2028 2058 2731 2761 2857 2887 4642 4672
Size: 2028 30 673 30 96 30 1755 30
Cuts at: 4672 5281 5311 5968 5998 6694 6724 6910 6940
Size: 609 30 657 30 696 30 186 30
Cuts at: 6940 7204 7234 7575 7605 8296 8326 10434 10464
Size: 264 30 341 30 691 30 2108 30
Cuts at: 10464 10522 10552 12515 12545 12653 12683 12845 12875
Size: 58 30 1963 30 108 30 162 30
Cuts at: 12875 14674 14704 16608
Size: 1799 30 1904
Fragments arranged by size:
2108 2028 1963 1904 1799 1755 696 691
673 657 609 341 264 186 162 108
96 58 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 152 688 1321 1348 1493 1510 1561 1744
Size: 152 536 633 27 145 17 51 183
Cuts at: 1744 1808 1852 2041 2176 2203 2257 2284 2311
Size: 64 44 189 135 27 54 27 27
Cuts at: 2311 2338 2365 2446 2473 2527 2620 2647 2752
Size: 27 27 81 27 54 93 27 105
Cuts at: 2752 2798 3053 3119 3487 3962 4063 4066 5047
Size: 46 255 66 368 475 101 3 981
Cuts at: 5047 6040 6918 7070 7139 9301 9379 10543 10597
Size: 993 878 152 69 2162 78 1164 54
Cuts at: 10597 11945 12043 12661 14161 14915 15121 15883 16528
Size: 1348 98 618 1500 754 206 762 645
Cuts at: 16528 16608
Size: 80
Fragments arranged by size:
2162 1500 1348 1164 993 981 878 762
754 645 633 618 536 475 368 255
206 189 183 152 152 145 135 105
101 98 93 81 80 78 69 66
64 54 54 54 51 46 44 27
27 27 27 27 27 27 27 17
3
BseYI C'CCAG_C
Cuts at: 0 407 2315 3249 3316 3327 3823 3882 6011
Size: 407 1908 934 67 11 496 59 2129
Cuts at: 6011 6630 7412 7438 7461 7468 7479 7783 8153
Size: 619 782 26 23 7 11 304 370
Cuts at: 8153 8167 8215 8944 9589 9815 9916 9974 9992
Size: 14 48 729 645 226 101 58 18
Cuts at: 9992 10103 10423 10460 10547 11332 11400 11510 11561
Size: 111 320 37 87 785 68 110 51
Cuts at: 11561 12087 12236 12242 12253 12973 12980 12998 13085
Size: 526 149 6 11 720 7 18 87
Cuts at: 13085 13369 13483 13513 13820 13938 16608
Size: 284 114 30 307 118 2670
Fragments arranged by size:
2670 2129 1908 934 785 782 729 720
645 619 526 496 407 370 320 307
304 284 226 149 118 114 111 110
101 87 87 68 67 59 58 51
48 37 30 26 23 18 18 14
11 11 11 7 7 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 707 1574 2422 2609 2952 3193 4804
Size: 507 200 867 848 187 343 241 1611
Cuts at: 4804 5126 6244 7838 9842 13918 15232 15505 16608
Size: 322 1118 1594 2004 4076 1314 273 1103
Fragments arranged by size:
4076 2004 1611 1594 1314 1118 1103 867
848 507 343 322 273 241 200 187
BsiEI CG_ry'CG
Cuts at: 0 1462 6295 15712 16608
Size: 1462 4833 9417 896
Fragments arranged by size:
9417 4833 1462 896
BsiHKAI G_wGCw'C
Cuts at: 0 201 650 3127 3461 3570 4198 4973 6062
Size: 201 449 2477 334 109 628 775 1089
Cuts at: 6062 6473 7188 7514 8403 8908 9203 9341 9641
Size: 411 715 326 889 505 295 138 300
Cuts at: 9641 9712 10114 10760 11501 12594 13600 13949 15173
Size: 71 402 646 741 1093 1006 349 1224
Cuts at: 15173 15274 15439 15875 16096 16142 16608
Size: 101 165 436 221 46 466
Fragments arranged by size:
2477 1224 1093 1089 1006 889 775 741
715 646 628 505 466 449 436 411
402 349 334 326 300 295 221 201
165 138 109 101 71 46
BsiWI C'GTAC_G
Cuts at: 0 5749 16608
Size: 5749 10859
BsmI GAATG_Cn'
Cuts at: 0 820 3087 3250 3517 6311 9843 16608
Size: 820 2267 163 267 2794 3532 6765
Fragments arranged by size:
6765 3532 2794 2267 820 267 163
BsmBI CGTCTCn'nnnn_
Cuts at: 0 868 1591 9122 16608
Size: 868 723 7531 7486
Fragments arranged by size:
7531 7486 868 723
Bsp1286I G_dGCh'C
Cuts at: 0 201 221 307 314 414 463 650 811
Size: 201 20 86 7 100 49 187 161
Cuts at: 811 988 1154 1199 1232 1283 3056 3127 3461
Size: 177 166 45 33 51 1773 71 334
Cuts at: 3461 3538 3570 3674 3685 3766 3954 3981 4198
Size: 77 32 104 11 81 188 27 217
Cuts at: 4198 4496 4718 4742 4889 4973 5002 5567 5698
Size: 298 222 24 147 84 29 565 131
Cuts at: 5698 5903 5923 6062 6333 6442 6473 6500 6557
Size: 205 20 139 271 109 31 27 57
Cuts at: 6557 6612 6778 6809 6899 7016 7024 7188 7514
Size: 55 166 31 90 117 8 164 326
Cuts at: 7514 7585 7700 7784 8168 8403 8674 8695 8908
Size: 71 115 84 384 235 271 21 213
Cuts at: 8908 9203 9283 9341 9425 9505 9530 9641 9712
Size: 295 80 58 84 80 25 111 71
Cuts at: 9712 9901 9957 10114 10147 10760 11390 11407 11455
Size: 189 56 157 33 613 630 17 48
Cuts at: 11455 11464 11501 11683 12039 12119 12389 12460 12594
Size: 9 37 182 356 80 270 71 134
Cuts at: 12594 12853 13272 13281 13323 13466 13600 13949 13989
Size: 259 419 9 42 143 134 349 40
Cuts at: 13989 14176 14205 14277 14464 14545 14647 14792 14892
Size: 187 29 72 187 81 102 145 100
Cuts at: 14892 15173 15186 15225 15274 15419 15439 15875 16014
Size: 281 13 39 49 145 20 436 139
Cuts at: 16014 16096 16142 16229 16431 16608
Size: 82 46 87 202 177
Fragments arranged by size:
1773 630 613 565 436 419 384 356
349 334 326 298 295 281 271 271
270 259 235 222 217 213 205 202
201 189 188 187 187 187 182 177
177 166 166 164 161 157 147 145
145 143 139 139 134 134 131 117
115 111 109 104 102 100 100 90
87 86 84 84 84 82 81 81
80 80 80 77 72 71 71 71
71 58 57 56 55 51 49 49
48 46 45 42 40 39 37 33
33 32 31 31 29 29 27 27
25 24 21 20 20 20 17 13
11 9 9 8 7
BspEI T'CCGG_A
Cuts at: 0 10739 16608
Size: 10739 5869
BspHI T'CATG_A
Cuts at: 0 6131 15102 15989 16608
Size: 6131 8971 887 619
Fragments arranged by size:
8971 6131 887 619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 104 384 2124 4522 5199 5207 5675
Size: 66 38 280 1740 2398 677 8 468
Cuts at: 5675 6185 7495 7860 9125 9149 9438 9587 9681
Size: 510 1310 365 1265 24 289 149 94
Cuts at: 9681 9940 12319 12401 13083 14030 16608
Size: 259 2379 82 682 947 2578
Fragments arranged by size:
2578 2398 2379 1740 1310 1265 947 682
677 510 468 365 289 280 259 149
94 82 66 38 24 8
BsrBI CCG'CTC
Cuts at: 0 554 1076 10440 12480 15015 15471 16608
Size: 554 522 9364 2040 2535 456 1137
Fragments arranged by size:
9364 2535 2040 1137 554 522 456
BsrDI GCAATG_nn'
Cuts at: 0 1243 1444 7390 9401 10193 10265 16608
Size: 1243 201 5946 2011 792 72 6343
Fragments arranged by size:
6343 5946 2011 1243 792 201 72
BsrFI r'CCGG_y
Cuts at: 0 42 2844 3030 3570 3935 5381 6294 6394
Size: 42 2802 186 540 365 1446 913 100
Cuts at: 6394 6432 8360 8808 8891 14180 16160 16307 16393
Size: 38 1928 448 83 5289 1980 147 86
Cuts at: 16393 16493 16608
Size: 100 115
Fragments arranged by size:
5289 2802 1980 1928 1446 913 540 448
365 186 147 115 100 100 86 83
42 38
BsrGI T'GTAC_A
Cuts at: 0 8646 10596 16608
Size: 8646 1950 6012
Fragments arranged by size:
8646 6012 1950
BssSI C'ACGA_G
Cuts at: 0 3211 3941 16608
Size: 3211 730 12667
Fragments arranged by size:
12667 3211 730
BstAPI GCAn_nnn'nTGC
Cuts at: 0 824 956 8125 9433 11178 11712 12549 13742
Size: 824 132 7169 1308 1745 534 837 1193
Cuts at: 13742 15320 15897 16137 16608
Size: 1578 577 240 471
Fragments arranged by size:
7169 1745 1578 1308 1193 837 824 577
534 471 240 132
BstBI TT'CG_AA
Cuts at: 0 4757 11115 16608
Size: 4757 6358 5493
Fragments arranged by size:
6358 5493 4757
BstEII G'GTnAC_C
Cuts at: 0 4509 16608
Size: 4509 12099
BstXI CCAn_nnnn'nTGG
Cuts at: 0 269 3048 3723 6946 8153 8931 10041 10284
Size: 269 2779 675 3223 1207 778 1110 243
Cuts at: 10284 12733 13204 15289 16608
Size: 2449 471 2085 1319
Fragments arranged by size:
3223 2779 2449 2085 1319 1207 1110 778
675 471 269 243
BstYI r'GATC_y
Cuts at: 0 500 2796 3620 7864 8078 8964 9613 10715
Size: 500 2296 824 4244 214 886 649 1102
Cuts at: 10715 11065 13128 14426 16562 16608
Size: 350 2063 1298 2136 46
Fragments arranged by size:
4244 2296 2136 2063 1298 1102 886 824
649 500 350 214 46
Bsu36I CC'TnA_GG
Cuts at: 0 164 362 1331 1358 1385 1412 1439 1493
Size: 164 198 969 27 27 27 27 54
Cuts at: 1493 1673 1727 1835 1862 1889 1943 1997 2024
Size: 180 54 108 27 27 54 54 27
Cuts at: 2024 2051 2078 2105 2186 2240 2267 2321 2348
Size: 27 27 27 81 54 27 54 27
Cuts at: 2348 2402 2456 2483 2537 2603 2630 5534 6930
Size: 54 54 27 54 66 27 2904 1396
Cuts at: 6930 7036 7613 9205 9284 9344 12417 12845 13034
Size: 106 577 1592 79 60 3073 428 189
Cuts at: 13034 13220 16608
Size: 186 3388
Fragments arranged by size:
3388 3073 2904 1592 1396 969 577 428
198 189 186 180 164 108 106 81
79 66 60 54 54 54 54 54
54 54 54 54 27 27 27 27
27 27 27 27 27 27 27 27
27 27
BtgI C'CryG_G
Cuts at: 0 568 917 3716 3954 4085 5216 5499 5960
Size: 568 349 2799 238 131 1131 283 461
Cuts at: 5960 6566 7493 7674 7884 9557 10034 10139 10911
Size: 606 927 181 210 1673 477 105 772
Cuts at: 10911 11603 11675 12342 15194 15617 16608
Size: 692 72 667 2852 423 991
Fragments arranged by size:
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667 606 568 477 461 423 349 283
238 210 181 131 105 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1610 3833 5438 8315 16608
Size: 1610 2223 1605 2877 8293
Fragments arranged by size:
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BtsI GCAGTG_nn'
Cuts at: 0 180 329 519 625 695 770 811 1315
Size: 180 149 190 106 70 75 41 504
Cuts at: 1315 3787 4198 4375 4438 4816 5755 7201 8141
Size: 2472 411 177 63 378 939 1446 940
Cuts at: 8141 9528 10404 10637 12363 13743 14408 14588 15000
Size: 1387 876 233 1726 1380 665 180 412
Cuts at: 15000 15602 15916 16608
Size: 602 314 692
Fragments arranged by size:
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CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 8247 8282 8428 8463 16608
Size: 8247 35 146 35 8145
Fragments arranged by size:
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DraI TTT'AAA
Cuts at: 0 11930 16608
Size: 11930 4678
DraIII CAC_nnn'GTG
Cuts at: 0 3544 4489 9601 11647 16608
Size: 3544 945 5112 2046 4961
Fragments arranged by size:
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DrdI GACnn_nn'nnGTC
Cuts at: 0 4114 16608
Size: 4114 12494
EaeI y'GGCC_r
Cuts at: 0 1459 4155 4296 5383 6657 8317 8532 8916
Size: 1459 2696 141 1087 1274 1660 215 384
Cuts at: 8916 9050 10431 10653 10908 12170 12611 15709 16301
Size: 134 1381 222 255 1262 441 3098 592
Cuts at: 16301 16442 16608
Size: 141 166
Fragments arranged by size:
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141 134
EagI C'GGCC_G
Cuts at: 0 1459 15709 16608
Size: 1459 14250 899
Fragments arranged by size:
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EarI CTCTTCn'nnn_
Cuts at: 0 1565 1751 1805 3551 4099 4538 4612 5272
Size: 1565 186 54 1746 548 439 74 660
Cuts at: 5272 5683 6478 6860 9011 9906 11117 12017 13253
Size: 411 795 382 2151 895 1211 900 1236
Cuts at: 13253 13285 13928 14005 14599 15850 16322 16608
Size: 32 643 77 594 1251 472 286
Fragments arranged by size:
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EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1475 5164 7154 14422 16608
Size: 1475 3689 1990 7268 2186
Fragments arranged by size:
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EcoICRI GAG'CTC
Cuts at: 0 6471 7512 9639 9710 10112 13598 15437 15873
Size: 6471 1041 2127 71 402 3486 1839 436
Cuts at: 15873 16608
Size: 735
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 416 691 1486 1564 1640 1721 1775
Size: 136 280 275 795 78 76 81 54
Cuts at: 1775 1937 2179 2953 2996 3067 3671 4688 5014
Size: 162 242 774 43 71 604 1017 326
Cuts at: 5014 5255 5308 5790 5989 6162 6445 6571 6973
Size: 241 53 482 199 173 283 126 402
Cuts at: 6973 7313 7442 8047 8129 8771 9109 9997 10591
Size: 340 129 605 82 642 338 888 594
Cuts at: 10591 11066 11300 11462 11608 11680 11716 11758 12053
Size: 475 234 162 146 72 36 42 295
Cuts at: 12053 12083 12517 12739 12802 13510 13952 13985 14305
Size: 30 434 222 63 708 442 33 320
Cuts at: 14305 14587 14623 14750 14921 15766 15880 16473 16608
Size: 282 36 127 171 845 114 593 135
Fragments arranged by size:
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54 53 43 42 36 36 33 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1303 3944 4123 6181 6782 8098 14635 14903
Size: 1303 2641 179 2058 601 1316 6537 268
Cuts at: 14903 16608
Size: 1705
Fragments arranged by size:
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179
EcoO109I rG'GnC_Cy
Cuts at: 0 72 310 339 366 401 459 758 1228
Size: 72 238 29 27 35 58 299 470
Cuts at: 1228 1229 1293 3297 4493 4548 4926 4998 4999
Size: 1 64 2004 1196 55 378 72 1
Cuts at: 4999 5134 5358 5402 5530 5664 5765 6519 6608
Size: 135 224 44 128 134 101 754 89
Cuts at: 6608 6609 6846 6896 7040 7524 7780 7992 8140
Size: 1 237 50 144 484 256 212 148
Cuts at: 8140 8288 8414 8584 8662 8691 8730 8763 8857
Size: 148 126 170 78 29 39 33 94
Cuts at: 8857 9000 9246 9271 9280 9747 9865 9920 10390
Size: 143 246 25 9 467 118 55 470
Cuts at: 10390 10933 11275 11305 11319 11386 11387 11404 11452
Size: 543 342 30 14 67 1 17 48
Cuts at: 11452 11460 11461 11532 12115 12116 12457 12523 12737
Size: 8 1 71 583 1 341 66 214
Cuts at: 12737 12759 12811 12835 12849 13022 13120 13354 13775
Size: 22 52 24 14 173 98 234 421
Cuts at: 13775 14151 14202 14273 14274 14289 14506 15415 15416
Size: 376 51 71 1 15 217 909 1
Cuts at: 15416 15540 15741 15750 16218 16242 16608
Size: 124 201 9 468 24 366
Fragments arranged by size:
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71 67 66 64 58 55 55 52
51 50 48 44 39 35 33 30
29 29 27 25 24 24 22 17
15 14 14 9 9 8 1 1
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 6670 16608
Size: 6670 9938
EcoRV GAT'ATC
Cuts at: 0 9189 10254 16608
Size: 9189 1065 6354
Fragments arranged by size:
9189 6354 1065
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 507 5623 9207 9564 12001 13562 16608
Size: 507 5116 3584 357 2437 1561 3046
Fragments arranged by size:
5116 3584 3046 2437 1561 507 357
FseI GG_CCGG'CC
Cuts at: 0 5385 16608
Size: 5385 11223
FspI TGC'GCA
Cuts at: 0 6617 16171 16608
Size: 6617 9554 437
Fragments arranged by size:
9554 6617 437
HaeII r_GCGC'y
Cuts at: 0 252 513 4693 4833 5216 6012 6638 8046
Size: 252 261 4180 140 383 796 626 1408
Cuts at: 8046 9294 11669 13795 15966 16608
Size: 1248 2375 2126 2171 642
Fragments arranged by size:
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Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 2893 2925 3328 3360 3372 3404
Size: 328 32 2533 32 403 32 12 32
Cuts at: 3404 3612 3644 3748 3780 4642 4674 5413 5445
Size: 208 32 104 32 862 32 739 32
Cuts at: 5445 5479 5511 5968 6000 6910 6942 7204 7236
Size: 34 32 457 32 910 32 262 32
Cuts at: 7236 7892 7924 8296 8328 12364 12396 12515 12547
Size: 656 32 372 32 4036 32 119 32
Cuts at: 12547 13439 13471 13948 13980 16608
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Fragments arranged by size:
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104 34 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 12
HincII GTy'rAC
Cuts at: 0 3615 3992 4224 6918 8889 16391 16608
Size: 3615 377 232 2694 1971 7502 217
Fragments arranged by size:
7502 3615 2694 1971 377 232 217
HindIII A'AGCT_T
Cuts at: 0 5388 6797 7295 8384 16608
Size: 5388 1409 498 1089 8224
Fragments arranged by size:
8224 5388 1409 1089 498
HpaI GTT'AAC
Cuts at: 0 3615 16608
Size: 3615 12993
KasI G'GCGC_C
Cuts at: 0 4689 4829 5212 6634 15962 16608
Size: 4689 140 383 1422 9328 646
Fragments arranged by size:
9328 4689 1422 646 383 140
KpnI G_GTAC'C
Cuts at: 0 7988 10102 13183 13511 15073 16608
Size: 7988 2114 3081 328 1562 1535
Fragments arranged by size:
7988 3081 2114 1562 1535 328
MfeI C'AATT_G
Cuts at: 0 10180 16608
Size: 10180 6428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1296 3629 4124 4331 7235 8863 9734
Size: 1268 28 2333 495 207 2904 1628 871
Cuts at: 9734 12070 12255 12540 16573 16608
Size: 2336 185 285 4033 35
Fragments arranged by size:
4033 2904 2336 2333 1628 1268 871 495
285 207 185 35 28
MscI TGG'CCA
Cuts at: 0 4298 6659 9052 10433 10910 12172 12613 16303
Size: 4298 2361 2393 1381 477 1262 441 3690
Cuts at: 16303 16608
Size: 305
Fragments arranged by size:
4298 3690 2393 2361 1381 1262 477 441
305
MslI CAynn'nnrTG
Cuts at: 0 422 561 2334 3046 3813 4135 4240 4723
Size: 422 139 1773 712 767 322 105 483
Cuts at: 4723 6944 9110 10039 10165 10330 11054 15199 16072
Size: 2221 2166 929 126 165 724 4145 873
Cuts at: 16072 16348 16424 16608
Size: 276 76 184
Fragments arranged by size:
4145 2221 2166 1773 929 873 767 724
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126 105 76
MspA1I CmG'CkG
Cuts at: 0 49 237 602 633 737 1043 1464 2157
Size: 49 188 365 31 104 306 421 693
Cuts at: 2157 2784 2833 3839 5106 5262 5997 6039 6160
Size: 627 49 1006 1267 156 735 42 121
Cuts at: 6160 6352 6740 7495 7790 7972 8116 8125 8153
Size: 192 388 755 295 182 144 9 28
Cuts at: 8153 8190 8611 9535 10200 10423 10518 10569 10840
Size: 37 421 924 665 223 95 51 271
Cuts at: 10840 10899 11157 11163 11184 11364 11514 11712 12267
Size: 59 258 6 21 180 150 198 555
Cuts at: 12267 12627 12681 12687 13659 13767 13824 15305 15507
Size: 360 54 6 972 108 57 1481 202
Cuts at: 15507 15567 16608
Size: 60 1041
Fragments arranged by size:
1481 1267 1041 1006 972 924 755 735
693 665 627 555 421 421 388 365
360 306 295 271 258 223 202 198
192 188 182 180 156 150 144 121
108 104 95 60 59 57 54 51
49 49 42 37 31 28 21 9
6 6
NaeI GCC'GGC
Cuts at: 0 3032 3937 5383 6396 6434 8362 16495 16608
Size: 3032 905 1446 1013 38 1928 8133 113
Fragments arranged by size:
8133 3032 1928 1446 1013 905 113 38
NarI GG'CG_CC
Cuts at: 0 4690 4830 5213 6635 15963 16608
Size: 4690 140 383 1422 9328 645
Fragments arranged by size:
9328 4690 1422 645 383 140
NcoI C'CATG_G
Cuts at: 0 917 3716 3954 7674 7884 10034 10139 10911
Size: 917 2799 238 3720 210 2150 105 772
Cuts at: 10911 11603 11675 12342 16608
Size: 692 72 667 4266
Fragments arranged by size:
4266 3720 2799 2150 917 772 692 667
238 210 105 72
NdeI CA'TA_TG
Cuts at: 0 7128 10061 16608
Size: 7128 2933 6547
Fragments arranged by size:
7128 6547 2933
NgoMIV G'CCGG_C
Cuts at: 0 3030 3935 5381 6394 6432 8360 16493 16608
Size: 3030 905 1446 1013 38 1928 8133 115
Fragments arranged by size:
8133 3030 1928 1446 1013 905 115 38
NheI G'CTAG_C
Cuts at: 0 10368 15493 16608
Size: 10368 5125 1115
Fragments arranged by size:
10368 5125 1115
NsiI A_TGCA'T
Cuts at: 0 4589 15404 16608
Size: 4589 10815 1204
Fragments arranged by size:
10815 4589 1204
NspI r_CATG'y
Cuts at: 0 112 1175 4134 4591 5298 8004 11400 12000
Size: 112 1063 2959 457 707 2706 3396 600
Cuts at: 12000 15084 15318 15369 15528 15837 15895 16077 16353
Size: 3084 234 51 159 309 58 182 276
Cuts at: 16353 16375 16608
Size: 22 233
Fragments arranged by size:
3396 3084 2959 2706 1063 707 600 457
309 276 234 233 182 159 112 58
51 22
PasI CC'CwG_GG
Cuts at: 0 295 307 1056 2656 6778 7561 7776 7939
Size: 295 12 749 1600 4122 783 215 163
Cuts at: 7939 9939 10972 11316 11382 11391 11457 12039 13272
Size: 2000 1033 344 66 9 66 582 1233
Cuts at: 13272 15213 16608
Size: 1941 1395
Fragments arranged by size:
4122 2000 1941 1600 1395 1233 1033 783
749 582 344 295 215 163 66 66
12 9
PciI A'CATG_T
Cuts at: 0 108 4130 5294 15524 16349 16371 16608
Size: 108 4022 1164 10230 825 22 237
Fragments arranged by size:
10230 4022 1164 825 237 108 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 1129 1192 3409 5392 6829 7068 7675
Size: 15 1114 63 2217 1983 1437 239 607
Cuts at: 7675 7945 8587 8602 8742 9601 11301 12396 12901
Size: 270 642 15 140 859 1700 1095 505
Cuts at: 12901 13753 14551 16221 16608
Size: 852 798 1670 387
Fragments arranged by size:
2217 1983 1700 1670 1437 1114 1095 859
852 798 642 607 505 387 270 239
140 63 15 15
PfoI T'CCnGG_A
Cuts at: 0 2930 3461 5852 6838 8517 10609 11277 12288
Size: 2930 531 2391 986 1679 2092 668 1011
Cuts at: 12288 12818 16608
Size: 530 3790
Fragments arranged by size:
3790 2930 2391 2092 1679 1011 986 668
531 530
PmlI CAC'GTG
Cuts at: 0 1200 3565 16608
Size: 1200 2365 13043
Fragments arranged by size:
13043 2365 1200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5281 5313 10522 10554 14434 14466 16608
Size: 5281 32 5209 32 3880 32 2142
Fragments arranged by size:
5281 5209 3880 2142 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 72 339 401 3297 4548 4926 5402 5530
Size: 72 267 62 2896 1251 378 476 128
Cuts at: 5530 5664 6519 7524 7992 8288 8414 8662 8730
Size: 134 855 1005 468 296 126 248 68
Cuts at: 8730 8857 9271 9747 9920 10390 11275 11305 12523
Size: 127 414 476 173 470 885 30 1218
Cuts at: 12523 12737 13022 13120 14289 14506 15750 16242 16608
Size: 214 285 98 1169 217 1244 492 366
Fragments arranged by size:
2896 1251 1244 1218 1169 1005 885 855
492 476 476 470 468 414 378 366
296 285 267 248 217 214 173 134
128 127 126 98 72 68 62 30
PshAI GACnn'nnGTC
Cuts at: 0 15083 16608
Size: 15083 1525
PspOMI G'GGCC_C
Cuts at: 0 310 459 1228 3977 4492 4998 6608 6895
Size: 310 149 769 2749 515 506 1610 287
Cuts at: 6895 7696 7780 8691 9279 11386 11403 11451 11460
Size: 801 84 911 588 2107 17 48 9
Cuts at: 11460 12115 12456 12849 14201 14273 15415 16608
Size: 655 341 393 1352 72 1142 1193
Fragments arranged by size:
2749 2107 1610 1352 1193 1142 911 801
769 655 588 515 506 393 341 310
287 149 84 72 48 17 9
PspXI vC'TCGA_Gb
Cuts at: 0 5506 16608
Size: 5506 11102
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 16177 16209 16608
Size: 16177 32 399
Fragments arranged by size:
16177 399 32
PstI C_TGCA'G
Cuts at: 0 63 175 632 838 5208 5672 5762 8121
Size: 63 112 457 206 4370 464 90 2359
Cuts at: 8121 8130 9678 9779 10248 10409 10922 11162 11168
Size: 9 1548 101 469 161 513 240 6
Cuts at: 11168 11201 12266 12272 12692 14373 16608
Size: 33 1065 6 420 1681 2235
Fragments arranged by size:
4370 2359 2235 1681 1548 1065 513 469
464 457 420 240 206 161 112 101
90 63 33 9 6 6
PvuII CAG'CTG
Cuts at: 0 49 633 737 2784 2833 6039 6160 7972
Size: 49 584 104 2047 49 3206 121 1812
Cuts at: 7972 8116 8125 8153 8190 8611 9535 10200 10423
Size: 144 9 28 37 421 924 665 223
Cuts at: 10423 10569 10840 10899 11157 11163 11184 11364 11514
Size: 146 271 59 258 6 21 180 150
Cuts at: 11514 11712 12267 12627 12687 13659 13767 13824 15305
Size: 198 555 360 60 972 108 57 1481
Cuts at: 15305 15567 16608
Size: 262 1041
Fragments arranged by size:
3206 2047 1812 1481 1041 972 924 665
584 555 421 360 271 262 258 223
198 180 150 146 144 121 108 104
60 59 57 49 49 37 28 21
9 6
RsrII CG'GwC_CG
Cuts at: 0 2210 16608
Size: 2210 14398
SacI G_AGCT'C
Cuts at: 0 6473 7514 9641 9712 10114 13600 15439 15875
Size: 6473 1041 2127 71 402 3486 1839 436
Cuts at: 15875 16608
Size: 733
Fragments arranged by size:
6473 3486 2127 1839 1041 733 436 402
71
SacII CC_GC'GG
Cuts at: 0 7496 16608
Size: 7496 9112
SanDI GG'GwC_CC
Cuts at: 0 5402 8414 9920 11305 12737 13022 16608
Size: 5402 3012 1506 1385 1432 285 3586
Fragments arranged by size:
5402 3586 3012 1506 1432 1385 285
SapI GCTCTTCn'nnn_
Cuts at: 0 3551 5272 5683 6478 6860 9011 9906 11117
Size: 3551 1721 411 795 382 2151 895 1211
Cuts at: 11117 13253 13928 14599 16322 16608
Size: 2136 675 671 1723 286
Fragments arranged by size:
3551 2151 2136 1723 1721 1211 895 795
675 671 411 382 286
SbfI CC_TGCA'GG
Cuts at: 0 838 16608
Size: 838 15770
ScaI AGT'ACT
Cuts at: 0 4747 16608
Size: 4747 11861
SexAI A'CCwGG_T
Cuts at: 0 10338 13196 15752 16608
Size: 10338 2858 2556 856
Fragments arranged by size:
10338 2858 2556 856
SfcI C'TryA_G
Cuts at: 0 59 171 628 834 5204 5668 5758 8117
Size: 59 112 457 206 4370 464 90 2359
Cuts at: 8117 8126 9674 9775 10244 10405 10558 10918 11158
Size: 9 1548 101 469 161 153 360 240
Cuts at: 11158 11164 11197 12262 12268 12661 12688 12887 13501
Size: 6 33 1065 6 393 27 199 614
Cuts at: 13501 14369 16122 16608
Size: 868 1753 486
Fragments arranged by size:
4370 2359 1753 1548 1065 868 614 486
469 464 457 393 360 240 206 199
161 153 112 101 90 59 33 27
9 6 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2845 11459 13642 16608
Size: 2845 8614 2183 2966
Fragments arranged by size:
8614 2966 2845 2183
SfoI GGC'GCC
Cuts at: 0 4691 4831 5214 6636 15964 16608
Size: 4691 140 383 1422 9328 644
Fragments arranged by size:
9328 4691 1422 644 383 140
SgrAI Cr'CCGG_yG
Cuts at: 0 3570 6432 16608
Size: 3570 2862 10176
Fragments arranged by size:
10176 3570 2862
SmaI CCC'GGG
Cuts at: 0 7025 11494 13972 14470 15924 16608
Size: 7025 4469 2478 498 1454 684
Fragments arranged by size:
7025 4469 2478 1454 684 498
SmlI C'TyrA_G
Cuts at: 0 1276 1594 2563 4252 4948 5506 6088 6478
Size: 1276 318 969 1689 696 558 582 390
Cuts at: 6478 9640 10687 12636 12938 14622 14932 16608
Size: 3162 1047 1949 302 1684 310 1676
Fragments arranged by size:
3162 1949 1689 1684 1676 1276 1047 969
696 582 558 390 318 310 302
SnaBI TAC'GTA
Cuts at: 0 4539 16608
Size: 4539 12069
SphI G_CATG'C
Cuts at: 0 1175 4591 11400 12000 15318 15895 16608
Size: 1175 3416 6809 600 3318 577 713
Fragments arranged by size:
6809 3416 3318 1175 713 600 577
SrfI GCCC'GGGC
Cuts at: 0 7025 14470 16608
Size: 7025 7445 2138
Fragments arranged by size:
7445 7025 2138
StuI AGG'CCT
Cuts at: 0 4303 5834 9544 11348 12376 13645 16608
Size: 4303 1531 3710 1804 1028 1269 2963
Fragments arranged by size:
4303 3710 2963 1804 1531 1269 1028
StyI C'CwwG_G
Cuts at: 0 384 917 1176 1217 3254 3351 3716 3954
Size: 384 533 259 41 2037 97 365 238
Cuts at: 3954 4238 4391 4899 5414 5660 6260 7520 7674
Size: 284 153 508 515 246 600 1260 154
Cuts at: 7674 7709 7884 8544 8852 9053 9275 9356 9413
Size: 35 175 660 308 201 222 81 57
Cuts at: 9413 9444 10034 10139 10911 11343 11603 11675 12342
Size: 31 590 105 772 432 260 72 667
Cuts at: 12342 12600 12900 13046 13123 13952 14277 14756 14792
Size: 258 300 146 77 829 325 479 36
Cuts at: 14792 14816 16238 16608
Size: 24 1422 370
Fragments arranged by size:
2037 1422 1260 829 772 667 660 600
590 533 515 508 479 432 384 370
365 325 308 300 284 260 259 258
246 238 222 201 175 154 153 146
105 97 81 77 72 57 41 36
35 31 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 930 1383 1752 1779 2835 2955 3603 5053
Size: 930 453 369 27 1056 120 648 1450
Cuts at: 5053 6305 6934 6952 7106 7775 8321 9028 10006
Size: 1252 629 18 154 669 546 707 978
Cuts at: 10006 13027 14676 15156 16608
Size: 3021 1649 480 1452
Fragments arranged by size:
3021 1649 1452 1450 1252 1056 978 930
707 669 648 629 546 480 453 369
154 120 27 18
TatI w'GTAC_w
Cuts at: 0 3778 4745 8646 10596 11283 16271 16608
Size: 3778 967 3901 1950 687 4988 337
Fragments arranged by size:
4988 3901 3778 1950 967 687 337
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2028 2060 6694 6726 7575 7607 8664 8696
Size: 2028 32 4634 32 849 32 1057 32
Cuts at: 8696 8841 8873 10434 10466 10591 10623 12845 12877
Size: 145 32 1561 32 125 32 2222 32
Cuts at: 12877 13199 13231 14064 14096 14324 14356 16608
Size: 322 32 833 32 228 32 2252
Fragments arranged by size:
4634 2252 2222 2028 1561 1057 849 833
322 228 145 125 32 32 32 32
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4654 4809 11487 13627 15754 16608
Size: 4654 155 6678 2140 2127 854
Fragments arranged by size:
6678 4654 2140 2127 854 155
XbaI T'CTAG_A
Cuts at: 0 14016 16608
Size: 14016 2592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 16 108 3961 4011 5814 10420 12328 12781
Size: 16 92 3853 50 1803 4606 1908 453
Cuts at: 12781 14814 14992 16608
Size: 2033 178 1616
Fragments arranged by size:
4606 3853 2033 1908 1803 1616 453 178
92 50 16
XhoI C'TCGA_G
Cuts at: 0 5506 9640 16608
Size: 5506 4134 6968
Fragments arranged by size:
6968 5506 4134
XmaI C'CCGG_G
Cuts at: 0 7023 11492 13970 14468 15922 16608
Size: 7023 4469 2478 498 1454 686
Fragments arranged by size:
7023 4469 2478 1454 686 498
XmnI GAAnn'nnTTC
Cuts at: 0 8756 16608
Size: 8756 7852
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AscI AsiSI BaeI BsaBI BssHII BstZ17I
ClaI FspAI MluI NotI NruI PacI PmeI PsiI
PvuI SalI SgfI SpeI SspI SwaI ZraI