Restriction map for
pFN21AE3506
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# NO CUT ENZYMES ( 45 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaLI
AscI BbeI BbrPI BcgI BseAI
BsiWI BsmBI BspEI BssHII BstAPI
BstEII BstPI CpoI EcoO65I EcoRV
FseI HpaI KasI MluI MroI
NarI NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfoI SgrAI SnaBI Sse8387I SwaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 6817 GACGTC GACGT^C
AcyI 6817 GACGTC G[GA]^CG[CT]C
AflII 2073 CTTAAG C^TTAAG
AocI 950 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2795 AGCGCT AGC^GCT
AviII 1175 TGCGCA TGC^GCA
AvrII 6948 CCTAGG C^CTAGG
BamHI 5824 GGATCC G^GATCC
BbvCI 6187 GCTGAGG CCTCAGC(-5/-2)
BciVI 4195 GTATCC GTATCC(6/5)
BfrI 2073 CTTAAG C^TTAAG
BlnI 6948 CCTAGG C^CTAGG
BlpI 4456 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4456 GCTCAGC GC^T[ACGT]AGC
BsaAI 4245 TACGTG [CT]AC^GT[GA]
BsaBI 7849 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6817 GACGTC G[GA]^CG[CT]C
BsiEI 133 CGGCCG CG[GA][CT]^CG
BspDI 2411 ATCGAT AT^CGAT
BspLU11I 617 ACATGT A^CATGT
BspMI 5045 GCAGGT ACCTGC(4/8)
BsrBI 130 GAGCGG CCGCTC(-3/-3)
Bst1107I 6397 GTATAC GTA^TAC
BstBI 4097 TTCGAA TT^CGAA
BstXI 6716 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6397 GTATAC GTA^TAC
Bsu36I 950 CCTTAGG CC^T[ACGT]AGG
CelII 4456 GCTCAGC GC^T[ACGT]AGC
ClaI 2411 ATCGAT AT^CGAT
DrdI 6811 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 133 CGGCCG C^GGCCG
EclXI 133 CGGCCG C^GGCCG
Eco47III 2795 AGCGCT AGC^GCT
Eco52I 133 CGGCCG C^GGCCG
Eco81I 950 CCTTAGG CC^T[ACGT]AGG
FspI 1175 TGCGCA TGC^GCA
HaeII 2795 AGCGCT [GA]GCGC^[CT]
Hin1I 6817 GACGTC G[GA]^CG[CT]C
HincII 4859 GTCAAC GT[CT]^[GA]AC
HindII 4859 GTCAAC GT[CT]^[GA]AC
MamI 7849 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3830 GCCGGC GCC^GGC
NgoMIV 3830 GCCGGC G^CCGGC
NspV 4097 TTCGAA TT^CGAA
PaeR7I 3944 CTCGAG C^TCGAG
SfiI 6248 GGCCCCATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4097 TTCGAA TT^CGAA
XcmI 674 CCATTGGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3944 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1896 GGTACC G^GTACC
3862 GGTACC
AflIII 617 ACATGT A^C[GA][CT]GT
7691 ACACGT
AhdI 5496 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6268 GACCGGCTGTC
ApaI 193 GGGCCC GGGCC^C
204 GGGCCC
AseI 630 ATTAAT AT^TAAT
2554 ATTAAT
AsnI 630 ATTAAT AT^TAAT
2554 ATTAAT
Asp718I 1896 GGTACC G^GTACC
3862 GGTACC
AspEI 5496 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6268 GACCGGCTGTC
AspI 485 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCCAGTC
BglI 206 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6249 GCCCCATAGGC
BsmFI 3112 GGGAC GGGAC(10/14)
6820 GTCCC
Bsp1407I 4373 TGTACA T^GTACA
6873 TGTACA
BsrGI 4373 TGTACA T^GTACA
6873 TGTACA
BssSI 304 CACGAG CACGAG(-5/-1)
7001 CACGAG
Eam1105I 5496 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6268 GACCGGCTGTC
EcoNI 7140 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
7191 CCTGTTAGAGG
KpnI 1896 GGTACC GGTAC^C
3862 GGTACC
KspI 208 CCGCGG CCGC^GG
252 CCGCGG
MfeI 1142 CAATTG C^AATTG
4125 CAATTG
MunI 1142 CAATTG C^AATTG
4125 CAATTG
PflFI 485 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCCAGTC
PpuMI 3112 GGGACCC [GA]G^G[AT]CC[CT]
5611 AGGACCT
PshBI 630 ATTAAT AT^TAAT
2554 ATTAAT
SacI 1087 GAGCTC GAGCT^C
2429 GAGCTC
SacII 208 CCGCGG CCGC^GG
252 CCGCGG
SmaI 190 CCCGGG CCC^GGG
3498 CCCGGG
SspBI 4373 TGTACA T^GTACA
6873 TGTACA
SspI 6632 AATATT AAT^ATT
7374 AATATT
StuI 1712 AGGCCT AGG^CCT
1810 AGGCCT
Tth111I 485 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCCAGTC
XbaI 4810 TCTAGA T^CTAGA
5550 TCTAGA
XmaCI 190 CCCGGG C^CCGGG
3498 CCCGGG
XmaI 190 CCCGGG C^CCGGG
3498 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshAI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SpeI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI