Restriction map for
pFN21AE3506
(Circular) (Six-base) MAPSORT of: pFN21AE3506.seq Check: 714 from: 1 to: 12565
pFN21AE3506 12565 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8826 10375 278
Size: 53 83 186 936 7290 1549 2468
Fragments arranged by size:
7290 2468 1549 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4907 5513 5685 5722 8403 9908 1937
Size: 2970 606 172 37 2681 1505 4594
Fragments arranged by size:
4594 2970 2681 1505 606 172 37
Acc65I G'GTAC_C
Cuts at: 3901 5867 9886 3901
Size: 1966 4019 6580
Fragments arranged by size:
6580 4019 1966
AclI AA'CG_TT
Cuts at: 1563 10692 11065 1563
Size: 9129 373 3063
Fragments arranged by size:
9129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2568 5503 6561 7806 8589 8673 9201 10628
Size: 936 2935 1058 1245 783 84 528 1427
Cuts at: 10628 12012 12280 1632
Size: 1384 268 1917
Fragments arranged by size:
2935 1917 1427 1384 1245 1058 936 783
528 268 84
AfeI AGC'GCT
Cuts at: 4802 4802
Size: 12565
AflII C'TTAA_G
Cuts at: 820 1017 4078 820
Size: 197 3061 9307
Fragments arranged by size:
9307 3061 197
AflIII A'CryG_T
Cuts at: 1203 2622 9696 11464 12441 1203
Size: 1419 7074 1768 977 1327
Fragments arranged by size:
7074 1768 1419 1327 977
AgeI A'CCGG_T
Cuts at: 11396 11396
Size: 12565
AhdI GACnn_n'nnGTC
Cuts at: 7506 8278 11294 7506
Size: 772 3016 8777
Fragments arranged by size:
8777 3016 772
AleI CACnn'nnGTG
Cuts at: 1166 8081 1166
Size: 6915 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 11650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 12533
AlwNI CAG_nnn'CTG
Cuts at: 3506 4058 4712 5592 6277 6514 7123 8791 10308
Size: 552 654 880 685 237 609 1668 1517
Cuts at: 10308 11880 3506
Size: 1572 4191
Fragments arranged by size:
4191 1668 1572 1517 880 685 654 609
552 237
ApaI G_GGCC'C
Cuts at: 2202 2213 2202
Size: 11 12554
ApaLI G'TGCA_C
Cuts at: 10622 11778 10622
Size: 1156 11409
ApoI r'AATT_y
Cuts at: 1493 2369 2383 2601 2613 3217 3435 3512 3997
Size: 876 14 218 12 604 218 77 485
Cuts at: 3997 4142 4329 4607 6099 7264 7331 7392 7521
Size: 145 187 278 1492 1165 67 61 129
Cuts at: 7521 7967 7991 8229 9011 9419 9506 9562 9874
Size: 446 24 238 782 408 87 56 312
Cuts at: 9874 10100 10252 12361 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1492 1165 876 782 604 485
446 408 312 278 238 226 218 218
187 152 145 129 87 77 67 61
56 24 14 12
AseI AT'TA_AT
Cuts at: 160 1032 2636 4560 11119 160
Size: 872 1604 1924 6559 1606
Fragments arranged by size:
6559 1924 1606 1604 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12565
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 5503 5949 9890 1870
Size: 73 252 3308 446 3941 4545
Fragments arranged by size:
4545 3941 3308 446 252 73
AvrII C'CTAG_G
Cuts at: 8953 8953
Size: 12565
BamHI G'GATC_C
Cuts at: 7829 9895 10262 7829
Size: 2066 367 10132
Fragments arranged by size:
10132 2066 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3901 4859 5867 9886 11341
Size: 325 217 627 2114 958 1008 4019 1455
Cuts at: 11341 618
Size: 1842
Fragments arranged by size:
4019 2114 1842 1455 1008 958 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2169 2202 2213 3096 4438 7143 9884 1391
Size: 778 33 11 883 1342 2705 2741 4072
Fragments arranged by size:
4072 2741 2705 1342 883 778 33 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5108 5784 5907 7105 928
Size: 971 3209 676 123 1198 6388
Fragments arranged by size:
6388 3209 1198 971 676 123
BbvCI CC'TCA_GC
Cuts at: 8193 8193
Size: 12565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10756 10790 1903
Size: 34 8819 34 3678
Fragments arranged by size:
8819 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6211 10457 11667 6211
Size: 4246 1210 7109
Fragments arranged by size:
7109 4246 1210
BclI T'GATC_A
Cuts at: 1548 2416 4411 4809 5359 6370 7351 7426 8059
Size: 868 1995 398 550 1011 981 75 633
Cuts at: 8059 8134 8940 1548
Size: 75 806 5173
Fragments arranged by size:
5173 1995 1011 981 868 806 633 550
398 75 75
BfrBI ATG'CAT
Cuts at: 3752 6991 7325 3752
Size: 3239 334 8992
Fragments arranged by size:
8992 3239 334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2217 8260 11176
Size: 107 122 71 631 605 545 6043 2916
Cuts at: 11176 136
Size: 1525
Fragments arranged by size:
6043 2916 1525 631 605 545 122 107
71
BglII A'GATC_T
Cuts at: 4203 4754 7434 8905 12560 4203
Size: 551 2680 1471 3655 4208
Fragments arranged by size:
4208 3655 2680 1471 551
BlpI GC'TnA_GC
Cuts at: 6462 9982 6462
Size: 3520 9045
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2213 6711 10626 11782 1790
Size: 412 11 4498 3915 1156 2573
Fragments arranged by size:
4498 3915 2573 1156 412 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3005 3693 3880
Size: 416 53 83 399 24 1588 688 187
Cuts at: 3880 5078 7034 7687 8141 10296 11254 442
Size: 1198 1956 653 454 2155 958 1753
Fragments arranged by size:
2155 1956 1753 1588 1198 958 688 653
454 416 399 187 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8218 8218
Size: 12565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3840 4092 5140 6825 8036 8705 8750 8813
Size: 2601 252 1048 1685 1211 669 45 63
Cuts at: 8813 8922 9595 11225 1239
Size: 109 673 1630 2579
Fragments arranged by size:
2601 2579 1685 1630 1211 1048 673 669
252 109 63 45
Bpu10I CC'TnA_GC
Cuts at: 2353 3090 8193 9543 9690 2353
Size: 737 5103 1350 147 5228
Fragments arranged by size:
5228 5103 1350 737 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4313 7355 8363 9112 10690 11555 11853 4313
Size: 3042 1008 749 1578 865 298 5025
Fragments arranged by size:
5025 3042 1578 1008 865 749 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2690 3632 6483 6668 7380 8003 11228
Size: 617 1191 942 2851 185 712 623 3225
Cuts at: 11228 882
Size: 2219
Fragments arranged by size:
3225 2851 2219 1191 942 712 623 617
185
BsaAI yAC'GTr
Cuts at: 493 1204 6252 10356 493
Size: 711 5048 4104 2702
Fragments arranged by size:
5048 4104 2702 711
BsaBI GATnn'nnATC
Cuts at: 9858 12559 9858
Size: 2701 9864
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8823 10372 10754 11389
Size: 53 83 186 936 7290 1549 382 635
Cuts at: 11389 275
Size: 1451
Fragments arranged by size:
7290 1549 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2270 3133 4962 10998 11396 11670 11817 12228 2270
Size: 863 1829 6036 398 274 147 411 2607
Fragments arranged by size:
6036 2607 1829 863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6144 6174 7125 7155 1178
Size: 30 165 30 4741 30 951 30 6588
Fragments arranged by size:
6588 4741 951 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2033 3740 3793 6513 7560 8049 9167
Size: 844 3 1707 53 2720 1047 489 1118
Cuts at: 9167 1186
Size: 4584
Fragments arranged by size:
4584 2720 1707 1118 1047 844 489 53
3
BseYI C'CCAG_C
Cuts at: 2189 3561 6604 9579 11768 12264 2189
Size: 1372 3043 2975 2189 496 2490
Fragments arranged by size:
3043 2975 2490 2189 1372 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2988 6440 7229 9608 1163
Size: 573 1252 3452 789 2379 4120
Fragments arranged by size:
4120 3452 2379 1252 789 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2141 10015 10776 11804 1540
Size: 275 186 140 7874 761 1028 2301
Fragments arranged by size:
7874 2301 1028 761 275 186 140
BsiHKAI G_wGCw'C
Cuts at: 2270 3096 3732 4438 4991 5529 8851 10626 10711
Size: 826 636 706 553 538 3322 1775 85
Cuts at: 10711 11782 2270
Size: 1071 3053
Fragments arranged by size:
3322 3053 1775 1071 826 706 636 553
538 85
BsmI GAATG_Cn'
Cuts at: 3283 7111 8030 8107 8455 8920 9052 10076 10169
Size: 3828 919 77 348 465 132 1024 93
Cuts at: 10169 3283
Size: 5679
Fragments arranged by size:
5679 3828 1024 919 465 348 132 93
77
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2202 2213 2270 3096 3732 4438
Size: 399 379 33 11 57 826 636 706
Cuts at: 4438 4991 5529 6711 7143 8851 9884 10626 10711
Size: 553 538 1182 432 1708 1033 742 85
Cuts at: 10711 11782 1391
Size: 1071 2174
Fragments arranged by size:
2174 1708 1182 1071 1033 826 742 706
636 553 538 432 399 379 85 57
33 11
BspEI T'CCGG_A
Cuts at: 12228 12228
Size: 12565
BspHI T'CATG_A
Cuts at: 2605 6006 9343 9373 10454 12184 2605
Size: 3401 3337 30 1081 1730 2986
Fragments arranged by size:
3401 3337 2986 1730 1081 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7041 9920 844
Size: 6197 2879 3489
Fragments arranged by size:
6197 3489 2879
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 10452 1394
Size: 429 314 8315 3507
Fragments arranged by size:
8315 3507 429 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 4611 5390 8594 8698 9117 11060 11234
Size: 1183 3363 779 3204 104 419 1943 174
Cuts at: 11234 65
Size: 1396
Fragments arranged by size:
3363 3204 1943 1396 1183 779 419 174
104
BsrFI r'CCGG_y
Cuts at: 1700 1810 4104 5835 8274 11209 11396 1700
Size: 110 2294 1731 2439 2935 187 2869
Fragments arranged by size:
2935 2869 2439 2294 1731 187 110
BsrGI T'GTAC_A
Cuts at: 96 6378 8878 96
Size: 6282 2500 3783
Fragments arranged by size:
6282 3783 2500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12565
BssSI C'ACGA_G
Cuts at: 2309 9006 10625 11637 2309
Size: 6697 1619 1012 3237
Fragments arranged by size:
6697 3237 1619 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 12565
BstBI TT'CG_AA
Cuts at: 6103 11369 6103
Size: 5266 7299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12565
BstXI CCAn_nnnn'nTGG
Cuts at: 8728 8728
Size: 12565
BstYI r'GATC_y
Cuts at: 1972 2668 3136 4203 4754 7434 7829 8197 8905
Size: 696 468 1067 551 2680 395 368 708
Cuts at: 8905 9394 9895 10262 10647 10664 12116 12202 12214
Size: 489 501 367 385 17 1452 86 12
Cuts at: 12214 12560 1972
Size: 346 1977
Fragments arranged by size:
2680 1977 1452 1067 708 696 551 501
489 468 395 385 368 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 8404 8404
Size: 12565
Bsu36I CC'TnA_GG
Cuts at: 2956 2956
Size: 12565
BtgI C'CryG_G
Cuts at: 513 1065 2004 2115 2169 2213 2257 2988 4494
Size: 552 939 111 54 44 44 731 1506
Cuts at: 4494 5196 513
Size: 702 7882
Fragments arranged by size:
7882 1506 939 731 702 552 111 54
44 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11826
Fragments arranged by size:
11826 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 3765 5119 5176 6856 9596 10081 10875 10895
Size: 1645 1354 57 1680 2740 485 794 20
Cuts at: 10895 2120
Size: 3790
Fragments arranged by size:
3790 2740 1680 1645 1354 794 485 57
20
ClaI AT'CG_AT
Cuts at: 1554 4417 1554
Size: 2863 9702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12530
DraI TTT'AAA
Cuts at: 2557 2737 4381 6828 9869 2557
Size: 180 1644 2447 3041 5253
Fragments arranged by size:
5253 3041 2447 1644 180
DraIII CAC_nnn'GTG
Cuts at: 2314 4658 6344 6743 2314
Size: 2344 1686 399 8136
Fragments arranged by size:
8136 2344 1686 399
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8822 11572 809
Size: 507 7506 2750 1802
Fragments arranged by size:
7506 2750 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2138 4062
Size: 54 1425 50 275 18 261 47 1924
Cuts at: 4062 4102 4225 5583 8718 10012 10901 12530 8
Size: 40 123 1358 3135 1294 889 1629 43
Fragments arranged by size:
3135 1924 1629 1425 1358 1294 889 275
261 123 54 50 47 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 2138 10012 1537
Size: 275 326 7874 4090
Fragments arranged by size:
7874 4090 326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3045 3551 4322 5277 6246 10495 1353
Size: 1692 506 771 955 969 4249 3423
Fragments arranged by size:
4249 3423 1692 969 955 771 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2241 11152 11526 11672 387
Size: 1064 138 652 8911 374 146 1280
Fragments arranged by size:
8911 1280 1064 652 374 146 138
EcoICRI GAG'CTC
Cuts at: 3094 4436 3094
Size: 1342 11223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 3840 4092 5140 5503 6561 6825
Size: 393 936 1272 252 1048 363 1058 264
Cuts at: 6825 7806 8036 8589 8673 8705 8750 8813 8922
Size: 981 230 553 84 32 45 63 109
Cuts at: 8922 9201 9595 10628 11225 12012 12280 1239
Size: 279 394 1033 597 787 268 1524
Fragments arranged by size:
1524 1272 1058 1048 1033 981 936 787
597 553 394 393 363 279 268 264
252 230 109 84 63 45 32
EcoNI CCTnn'n_nnAGG
Cuts at: 9149 9200 9149
Size: 51 12514
EcoO109I rG'GnC_Cy
Cuts at: 2080 2209 5118 7617 2080
Size: 129 2909 2499 7028
Fragments arranged by size:
7028 2909 2499 129
EcoRI G'AATT_C
Cuts at: 2369 3217 6099 7331 9562 9874 10252 2369
Size: 848 2882 1232 2231 312 378 4682
Fragments arranged by size:
4682 2882 2231 1232 848 378 312
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6998 8515 8615 8695 9568 6998
Size: 1517 100 80 873 9995
Fragments arranged by size:
9995 1517 873 100 80
FspI TGC'GCA
Cuts at: 3182 11071 3182
Size: 7889 4676
HaeII r_GCGC'y
Cuts at: 1678 4804 11712 1678
Size: 3126 6908 2531
Fragments arranged by size:
6908 3126 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 3083 3115 3205
Size: 32 135 32 836 32 643 32 90
Cuts at: 3205 3237 4024 4056 4577 4609 4814 4846 5655
Size: 32 787 32 521 32 205 32 809
Cuts at: 5655 5687 6221 6253 7037 7069 7492 7524 8126
Size: 32 534 32 784 32 423 32 602
Cuts at: 8126 8158 8264 8296 9050 9082 11206 11238 11280
Size: 32 106 32 754 32 2124 32 42
Cuts at: 11280 11312 1373
Size: 32 2626
Fragments arranged by size:
2626 2124 836 809 787 784 754 643
602 534 521 423 205 135 106 90
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6866 9909 10155 669
Size: 988 281 4928 3043 246 3079
Fragments arranged by size:
4928 3079 3043 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3593 5312 7473 748
Size: 2845 1719 2161 5840
Fragments arranged by size:
5840 2845 2161 1719
HpaI GTT'AAC
Cuts at: 10155 10155
Size: 12565
KpnI G_GTAC'C
Cuts at: 3905 5871 9890 3905
Size: 1966 4019 6580
Fragments arranged by size:
6580 4019 1966
MfeI C'AATT_G
Cuts at: 3147 6130 10164 3147
Size: 2983 4034 5548
Fragments arranged by size:
5548 4034 2983
MluI A'CGCG_T
Cuts at: 12441 12441
Size: 12565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3154 3713 5728 6506 8793 9388 9738 11679
Size: 1849 559 2015 778 2287 595 350 1941
Cuts at: 11679 11863 1305
Size: 184 2007
Fragments arranged by size:
2287 2015 2007 1941 1849 778 595 559
350 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 4227 5585 8720 10
Size: 54 1425 343 261 2134 1358 3135 3855
Fragments arranged by size:
3855 3135 2134 1425 1358 343 261 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2627 3204 4941 6414 7458 8081 9057
Size: 648 1461 577 1737 1473 1044 623 976
Cuts at: 9057 9254 10523 10882 11041 518
Size: 197 1269 359 159 2042
Fragments arranged by size:
2042 1737 1473 1461 1269 1044 976 648
623 577 359 197 159
MspA1I CmG'CkG
Cuts at: 1606 2178 2215 2259 3503 4055 5570 6376 6466
Size: 572 37 44 1244 552 1515 806 90
Cuts at: 6466 6665 6845 7123 9981 10658 11806 12051 1606
Size: 199 180 278 2858 677 1148 245 2120
Fragments arranged by size:
2858 2120 1515 1244 1148 806 677 572
552 278 245 199 180 90 44 37
NaeI GCC'GGC
Cuts at: 5837 5837
Size: 12565
NcoI C'CATG_G
Cuts at: 513 1065 2004 2169 4494 5196 513
Size: 552 939 165 2325 702 7882
Fragments arranged by size:
7882 2325 939 702 552 165
NdeI CA'TA_TG
Cuts at: 387 3754 4348 5191 7327 387
Size: 3367 594 843 2136 5625
Fragments arranged by size:
5625 3367 2136 843 594
NgoMIV G'CCGG_C
Cuts at: 5835 5835
Size: 12565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12565
NotI GC'GGCC_GC
Cuts at: 10012 10012
Size: 12565
NsiI A_TGCA'T
Cuts at: 3754 6993 7327 3754
Size: 3239 334 8992
Fragments arranged by size:
8992 3239 334
NspI r_CATG'y
Cuts at: 1132 1229 2626 3124 3187 4679 5260 6173 9923
Size: 97 1397 498 63 1492 581 913 3750
Cuts at: 9923 11468 1132
Size: 1545 2229
Fragments arranged by size:
3750 2229 1545 1492 1397 913 581 498
97 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12565
PciI A'CATG_T
Cuts at: 2622 11464 2622
Size: 8842 3723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 5068 6277 1108
Size: 1206 2754 1209 7396
Fragments arranged by size:
7396 2754 1209 1206
PfoI T'CCnGG_A
Cuts at: 3030 3030
Size: 12565
PmeI GTTT'AAAC
Cuts at: 9869 9869
Size: 12565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7536 7568 10634 10666 12147 12179 7536
Size: 32 3066 32 1481 32 7922
Fragments arranged by size:
7922 3066 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5118 7617 5118
Size: 2499 10066
PshAI GACnn'nnGTC
Cuts at: 1613 5854 5896 6193 9483 1613
Size: 4241 42 297 3290 4695
Fragments arranged by size:
4695 4241 3290 297 42
PsiI TTA'TAA
Cuts at: 6971 10135 6971
Size: 3164 9401
PspOMI G'GGCC_C
Cuts at: 2198 2209 2198
Size: 11 12554
PspXI vC'TCGA_Gb
Cuts at: 5949 5949
Size: 12565
PstI C_TGCA'G
Cuts at: 830 3502 6034 7248 9541 9917 830
Size: 2672 2532 1214 2293 376 3478
Fragments arranged by size:
3478 2672 2532 2293 1214 376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12565
PvuII CAG'CTG
Cuts at: 4055 5570 6376 6466 6665 6845 7123 4055
Size: 1515 806 90 199 180 278 9497
Fragments arranged by size:
9497 1515 806 278 199 180 90
SacI G_AGCT'C
Cuts at: 3096 4438 3096
Size: 1342 11223
SacII CC_GC'GG
Cuts at: 2216 2260 2216
Size: 44 12521
SalI G'TCGA_C
Cuts at: 1936 9907 1936
Size: 7971 4594
SanDI GG'GwC_CC
Cuts at: 5118 5118
Size: 12565
SapI GCTCTTCn'nnn_
Cuts at: 3551 4322 5277 3551
Size: 771 955 10839
Fragments arranged by size:
10839 955 771
SbfI CC_TGCA'GG
Cuts at: 9917 9917
Size: 12565
ScaI AGT'ACT
Cuts at: 7250 8855 9551 10813 12525 7250
Size: 1605 696 1262 1712 7290
Fragments arranged by size:
7290 1712 1605 1262 696
SfcI C'TryA_G
Cuts at: 826 1045 3282 3498 4549 4725 4815 5723 6030
Size: 219 2237 216 1051 176 90 908 307
Cuts at: 6030 6257 7244 9537 9596 9797 9913 11048 11729
Size: 227 987 2293 59 201 116 1135 681
Cuts at: 11729 11920 826
Size: 191 1471
Fragments arranged by size:
2293 2237 1471 1135 1051 987 908 681
307 227 219 216 201 191 176 116
90 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 8260 8260
Size: 12565
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12565
SmaI CCC'GGG
Cuts at: 1872 2197 5505 9892 1872
Size: 325 3308 4387 4545
Fragments arranged by size:
4545 4387 3308 325
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4078 4292 5949 7370 8378 9127
Size: 197 926 2135 214 1657 1421 1008 749
Cuts at: 9127 10669 11570 11832 820
Size: 1542 901 262 1553
Fragments arranged by size:
2135 1657 1553 1542 1421 1008 926 901
749 262 214 197
SnaBI TAC'GTA
Cuts at: 493 10356 493
Size: 9863 2702
SpeI A'CTAG_T
Cuts at: 152 4093 6485 7340 152
Size: 3941 2392 855 5377
Fragments arranged by size:
5377 3941 2392 855
SphI G_CATG'C
Cuts at: 1132 3124 3187 6173 9923 1132
Size: 1992 63 2986 3750 3774
Fragments arranged by size:
3774 3750 2986 1992 63
SspI AAT'ATT
Cuts at: 5 52 8639 9381 10489 5
Size: 47 8587 742 1108 2081
Fragments arranged by size:
8587 2081 1108 742 47
StuI AGG'CCT
Cuts at: 1517 3719 3817 1517
Size: 2202 98 10265
Fragments arranged by size:
10265 2202 98
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 3720 4197 4494 5196 5288
Size: 552 939 165 1551 477 297 702 92
Cuts at: 5288 7017 8953 10004 513
Size: 1729 1936 1051 3074
Fragments arranged by size:
3074 1936 1729 1551 1051 939 702 552
477 297 165 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 3886 9124 10592 10609 10762 10947
Size: 1255 1101 1205 5238 1468 17 153 185
Cuts at: 10947 11392 325
Size: 445 1498
Fragments arranged by size:
5238 1498 1468 1255 1205 1101 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6378 6890 7248
Size: 274 80 33 51 1443 4401 512 358
Cuts at: 7248 8853 8878 9264 9549 10811 12523 96
Size: 1605 25 386 285 1262 1712 138
Fragments arranged by size:
4401 1712 1605 1443 1262 512 386 358
285 274 138 80 51 33 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2832 2864 7165 7197 7904 7936 8313 8345 12525
Size: 32 4301 32 707 32 377 32 4180
Cuts at: 12525 12557 2832
Size: 32 2840
Fragments arranged by size:
4301 4180 2840 707 377 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2493 3011 1316
Size: 1177 518 10870
Fragments arranged by size:
10870 1177 518
XbaI T'CTAG_A
Cuts at: 6815 7555 9901 6815
Size: 740 2346 9479
Fragments arranged by size:
9479 2346 740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2686
Size: 12565
XhoI C'TCGA_G
Cuts at: 1943 5949 1943
Size: 4006 8559
XmaI C'CCGG_G
Cuts at: 1870 2195 5503 9890 1870
Size: 325 3308 4387 4545
Fragments arranged by size:
4545 4387 3308 325
XmnI GAAnn'nnTTC
Cuts at: 4530 7277 9359 9682 10694 4530
Size: 2747 2082 323 1012 6401
Fragments arranged by size:
6401 2747 2082 1012 323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8824 10373 276
Size: 53 83 186 936 7290 1549 2468
Fragments arranged by size:
7290 2468 1549 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BsiWI BsmBI EcoRV FseI
FspAI KasI NarI NruI PacI PmlI PsrI RsrII
SexAI SfoI SrfI SwaI