(Linear) (Six-base) MAPSORT of: KIEE3506.seq Check: 2084 from: 1 to: 7860 KIEE3506.orf With 182 enzymes: SgfI * July 3, 2012 17:59 .. AatII G_ACGT'C Cuts at: 0 6821 7860 Size: 6821 1039 AccI GT'mk_AC Cuts at: 0 2902 3508 3680 3717 6398 7860 Size: 2902 606 172 37 2681 1462 Fragments arranged by size: 2902 2681 1462 606 172 37 Acc65I G'GTAC_C Cuts at: 0 1896 3862 7860 Size: 1896 1966 3998 Fragments arranged by size: 3998 1966 1896 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 563 3498 4556 5801 6584 6668 7196 7860 Size: 563 2935 1058 1245 783 84 528 664 Fragments arranged by size: 2935 1245 1058 783 664 563 528 84 AfeI AGC'GCT Cuts at: 0 2797 7860 Size: 2797 5063 AflII C'TTAA_G Cuts at: 0 2073 7860 Size: 2073 5787 AflIII A'CryG_T Cuts at: 0 617 7691 7860 Size: 617 7074 169 Fragments arranged by size: 7074 617 169 AhdI GACnn_n'nnGTC Cuts at: 0 5501 6273 7860 Size: 5501 772 1587 Fragments arranged by size: 5501 1587 772 AleI CACnn'nnGTG Cuts at: 0 6076 7860 Size: 6076 1784 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 94 7860 Size: 94 7766 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 4163 4195 7860 Size: 4163 32 3665 Fragments arranged by size: 4163 3665 32 AlwNI CAG_nnn'CTG Cuts at: 0 1501 2053 2707 3587 4272 4509 5118 6786 Size: 1501 552 654 880 685 237 609 1668 Cuts at: 6786 7860 Size: 1074 Fragments arranged by size: 1668 1501 1074 880 685 654 609 552 237 ApaI G_GGCC'C Cuts at: 0 197 208 7860 Size: 197 11 7652 Fragments arranged by size: 7652 197 11 ApoI r'AATT_y Cuts at: 0 364 378 596 608 1212 1430 1507 1992 Size: 364 14 218 12 604 218 77 485 Cuts at: 1992 2137 2324 2602 4094 5259 5326 5387 5516 Size: 145 187 278 1492 1165 67 61 129 Cuts at: 5516 5962 5986 6224 7006 7414 7501 7557 7860 Size: 446 24 238 782 408 87 56 303 Fragments arranged by size: 1492 1165 782 604 485 446 408 364 303 278 238 218 218 187 145 129 87 77 67 61 56 24 14 12 AseI AT'TA_AT Cuts at: 0 631 2555 7860 Size: 631 1924 5305 Fragments arranged by size: 5305 1924 631 AvaI C'yCGr_G Cuts at: 0 190 3498 3944 7860 Size: 190 3308 446 3916 Fragments arranged by size: 3916 3308 446 190 AvrII C'CTAG_G Cuts at: 0 6948 7860 Size: 6948 912 BamHI G'GATC_C Cuts at: 0 5824 7860 Size: 5824 2036 BanI G'GyrC_C Cuts at: 0 1896 2854 3862 7860 Size: 1896 958 1008 3998 Fragments arranged by size: 3998 1896 1008 958 BanII G_rGCy'C Cuts at: 0 164 197 208 1091 2433 5138 7860 Size: 164 33 11 883 1342 2705 2722 Fragments arranged by size: 2722 2705 1342 883 164 33 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 3103 3779 3902 5100 7860 Size: 3103 676 123 1198 2760 Fragments arranged by size: 3103 2760 1198 676 123 BbvCI CC'TCA_GC Cuts at: 0 6188 7860 Size: 6188 1672 BciVI GTATCCnnnnn_n' Cuts at: 0 4206 7860 Size: 4206 3654 BclI T'GATC_A Cuts at: 0 411 2406 2804 3354 4365 5346 5421 6054 Size: 411 1995 398 550 1011 981 75 633 Cuts at: 6054 6129 6935 7860 Size: 75 806 925 Fragments arranged by size: 1995 1011 981 925 806 633 550 411 398 75 75 BfrBI ATG'CAT Cuts at: 0 1747 4986 5320 7860 Size: 1747 3239 334 2540 Fragments arranged by size: 3239 2540 1747 334 BglI GCCn_nnn'nGGC Cuts at: 0 212 6255 7860 Size: 212 6043 1605 Fragments arranged by size: 6043 1605 212 BglII A'GATC_T Cuts at: 0 2198 2749 5429 6900 7860 Size: 2198 551 2680 1471 960 Fragments arranged by size: 2680 2198 1471 960 551 BlpI GC'TnA_GC Cuts at: 0 4457 7860 Size: 4457 3403 Bme1580I G_kGCm'C Cuts at: 0 197 208 4706 7860 Size: 197 11 4498 3154 Fragments arranged by size: 4498 3154 197 11 BmrI ACTGGGnnnn_n' Cuts at: 0 1000 1688 1875 3073 5029 5682 6136 7860 Size: 1000 688 187 1198 1956 653 454 1724 Fragments arranged by size: 1956 1724 1198 1000 688 653 454 187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 6213 7860 Size: 6213 1647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1835 2087 3135 4820 6031 6700 6745 6808 Size: 1835 252 1048 1685 1211 669 45 63 Cuts at: 6808 6917 7590 7860 Size: 109 673 270 Fragments arranged by size: 1835 1685 1211 1048 673 669 270 252 109 63 45 Bpu10I CC'TnA_GC Cuts at: 0 348 1085 6188 7538 7685 7860 Size: 348 737 5103 1350 147 175 Fragments arranged by size: 5103 1350 737 348 175 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2308 5350 6358 7107 7860 Size: 2308 3042 1008 749 753 Fragments arranged by size: 3042 2308 1008 753 749 BsaI GGTCTCn'nnnn_ Cuts at: 0 685 1627 4478 4663 5375 5998 7860 Size: 685 942 2851 185 712 623 1862 Fragments arranged by size: 2851 1862 942 712 685 623 185 BsaAI yAC'GTr Cuts at: 0 4247 7860 Size: 4247 3613 BsaBI GATnn'nnATC Cuts at: 0 7853 7860 Size: 7853 7 BsaHI Gr'CG_yC Cuts at: 0 6818 7860 Size: 6818 1042 BsaWI w'CCGG_w Cuts at: 0 265 1128 2957 7860 Size: 265 863 1829 4903 Fragments arranged by size: 4903 1829 863 265 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4139 4169 5120 5150 7860 Size: 4139 30 951 30 2710 Fragments arranged by size: 4139 2710 951 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 28 1735 1788 4508 5555 6044 7162 Size: 25 3 1707 53 2720 1047 489 1118 Cuts at: 7162 7860 Size: 698 Fragments arranged by size: 2720 1707 1118 1047 698 489 53 25 3 BseYI C'CCAG_C Cuts at: 0 184 1556 4599 7574 7860 Size: 184 1372 3043 2975 286 Fragments arranged by size: 3043 2975 1372 286 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 983 4435 5224 7603 7860 Size: 983 3452 789 2379 257 Fragments arranged by size: 3452 2379 983 789 257 BsiEI CG_ry'CG Cuts at: 0 136 7860 Size: 136 7724 BsiHKAI G_wGCw'C Cuts at: 0 265 1091 1727 2433 2986 3524 6846 7860 Size: 265 826 636 706 553 538 3322 1014 Fragments arranged by size: 3322 1014 826 706 636 553 538 265 BsmI GAATG_Cn' Cuts at: 0 1278 5106 6025 6102 6450 6915 7047 7860 Size: 1278 3828 919 77 348 465 132 813 Fragments arranged by size: 3828 1278 919 813 465 348 132 77 Bsp1286I G_dGCh'C Cuts at: 0 164 197 208 265 1091 1727 2433 2986 Size: 164 33 11 57 826 636 706 553 Cuts at: 2986 3524 4706 5138 6846 7860 Size: 538 1182 432 1708 1014 Fragments arranged by size: 1708 1182 1014 826 706 636 553 538 432 164 57 33 11 BspHI T'CATG_A Cuts at: 0 600 4001 7338 7368 7860 Size: 600 3401 3337 30 492 Fragments arranged by size: 3401 3337 600 492 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 5036 7860 Size: 5036 2824 BsrBI CCG'CTC Cuts at: 0 132 7860 Size: 132 7728 BsrDI GCAATG_nn' Cuts at: 0 2606 3385 6589 6693 7112 7860 Size: 2606 779 3204 104 419 748 Fragments arranged by size: 3204 2606 779 748 419 104 BsrFI r'CCGG_y Cuts at: 0 2099 3830 6269 7860 Size: 2099 1731 2439 1591 Fragments arranged by size: 2439 2099 1731 1591 BsrGI T'GTAC_A Cuts at: 0 4373 6873 7860 Size: 4373 2500 987 Fragments arranged by size: 4373 2500 987 BssSI C'ACGA_G Cuts at: 0 304 7001 7860 Size: 304 6697 859 Fragments arranged by size: 6697 859 304 BstBI TT'CG_AA Cuts at: 0 4098 7860 Size: 4098 3762 BstXI CCAn_nnnn'nTGG Cuts at: 0 6723 7860 Size: 6723 1137 BstYI r'GATC_y Cuts at: 0 663 1131 2198 2749 5429 5824 6192 6900 Size: 663 468 1067 551 2680 395 368 708 Cuts at: 6900 7389 7860 Size: 489 471 Fragments arranged by size: 2680 1067 708 663 551 489 471 468 395 368 BstZ17I GTA'TAC Cuts at: 0 6399 7860 Size: 6399 1461 Bsu36I CC'TnA_GG Cuts at: 0 951 7860 Size: 951 6909 BtgI C'CryG_G Cuts at: 0 110 164 208 252 983 2489 3191 7860 Size: 110 54 44 44 731 1506 702 4669 Fragments arranged by size: 4669 1506 731 702 110 54 44 44 BtsI GCAGTG_nn' Cuts at: 0 115 1760 3114 3171 4851 7591 7860 Size: 115 1645 1354 57 1680 2740 269 Fragments arranged by size: 2740 1680 1645 1354 269 115 57 ClaI AT'CG_AT Cuts at: 0 2412 7860 Size: 2412 5448 DraI TTT'AAA Cuts at: 0 552 732 2376 4823 7860 Size: 552 180 1644 2447 3037 Fragments arranged by size: 3037 2447 1644 552 180 DraIII CAC_nnn'GTG Cuts at: 0 309 2653 4339 4738 7860 Size: 309 2344 1686 399 3122 Fragments arranged by size: 3122 2344 1686 399 309 DrdI GACnn_nn'nnGTC Cuts at: 0 6817 7860 Size: 6817 1043 EaeI y'GGCC_r Cuts at: 0 86 133 2057 2097 2220 3578 6713 7860 Size: 86 47 1924 40 123 1358 3135 1147 Fragments arranged by size: 3135 1924 1358 1147 123 86 47 40 EagI C'GGCC_G Cuts at: 0 133 7860 Size: 133 7727 EarI CTCTTCn'nnn_ Cuts at: 0 1040 1546 2317 3272 4241 7860 Size: 1040 506 771 955 969 3619 Fragments arranged by size: 3619 1040 969 955 771 506 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 236 7860 Size: 236 7624 EcoICRI GAG'CTC Cuts at: 0 1089 2431 7860 Size: 1089 1342 5429 Fragments arranged by size: 5429 1342 1089 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 563 1835 2087 3135 3498 4556 4820 5801 Size: 563 1272 252 1048 363 1058 264 981 Cuts at: 5801 6031 6584 6668 6700 6745 6808 6917 7196 Size: 230 553 84 32 45 63 109 279 Cuts at: 7196 7590 7860 Size: 394 270 Fragments arranged by size: 1272 1058 1048 981 563 553 394 363 279 270 264 252 230 109 84 63 45 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 7144 7195 7860 Size: 7144 51 665 Fragments arranged by size: 7144 665 51 EcoO109I rG'GnC_Cy Cuts at: 0 75 204 3113 5612 7860 Size: 75 129 2909 2499 2248 Fragments arranged by size: 2909 2499 2248 129 75 EcoRI G'AATT_C Cuts at: 0 364 1212 4094 5326 7557 7860 Size: 364 848 2882 1232 2231 303 Fragments arranged by size: 2882 2231 1232 848 364 303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4993 6510 6610 6690 7563 7860 Size: 4993 1517 100 80 873 297 Fragments arranged by size: 4993 1517 873 297 100 80 FspI TGC'GCA Cuts at: 0 1177 7860 Size: 1177 6683 HaeII r_GCGC'y Cuts at: 0 2799 7860 Size: 2799 5061 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 403 435 1078 1110 1200 1232 2019 2051 Size: 403 32 643 32 90 32 787 32 Cuts at: 2051 2572 2604 2809 2841 3650 3682 4216 4248 Size: 521 32 205 32 809 32 534 32 Cuts at: 4248 5032 5064 5487 5519 6121 6153 6259 6291 Size: 784 32 423 32 602 32 106 32 Cuts at: 6291 7045 7077 7860 Size: 754 32 783 Fragments arranged by size: 809 787 784 783 754 643 602 534 521 423 403 205 106 90 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 4861 7860 Size: 4861 2999 HindIII A'AGCT_T Cuts at: 0 1588 3307 5468 7860 Size: 1588 1719 2161 2392 Fragments arranged by size: 2392 2161 1719 1588 KpnI G_GTAC'C Cuts at: 0 1900 3866 7860 Size: 1900 1966 3994 Fragments arranged by size: 3994 1966 1900 MfeI C'AATT_G Cuts at: 0 1142 4125 7860 Size: 1142 2983 3735 Fragments arranged by size: 3735 2983 1142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1149 1708 3723 4501 6788 7383 7733 7860 Size: 1149 559 2015 778 2287 595 350 127 Fragments arranged by size: 2287 2015 1149 778 595 559 350 127 MscI TGG'CCA Cuts at: 0 88 2222 3580 6715 7860 Size: 88 2134 1358 3135 1145 Fragments arranged by size: 3135 2134 1358 1145 88 MslI CAynn'nnrTG Cuts at: 0 622 1199 2936 4409 5453 6076 7052 7249 Size: 622 577 1737 1473 1044 623 976 197 Cuts at: 7249 7860 Size: 611 Fragments arranged by size: 1737 1473 1044 976 623 622 611 577 197 MspA1I CmG'CkG Cuts at: 0 173 210 254 1498 2050 3565 4371 4461 Size: 173 37 44 1244 552 1515 806 90 Cuts at: 4461 4660 4840 5118 7860 Size: 199 180 278 2742 Fragments arranged by size: 2742 1515 1244 806 552 278 199 180 173 90 44 37 NaeI GCC'GGC Cuts at: 0 3832 7860 Size: 3832 4028 NcoI C'CATG_G Cuts at: 0 164 2489 3191 7860 Size: 164 2325 702 4669 Fragments arranged by size: 4669 2325 702 164 NdeI CA'TA_TG Cuts at: 0 1749 2343 3186 5322 7860 Size: 1749 594 843 2136 2538 Fragments arranged by size: 2538 2136 1749 843 594 NgoMIV G'CCGG_C Cuts at: 0 3830 7860 Size: 3830 4030 NsiI A_TGCA'T Cuts at: 0 1749 4988 5322 7860 Size: 1749 3239 334 2538 Fragments arranged by size: 3239 2538 1749 334 NspI r_CATG'y Cuts at: 0 621 1119 1182 2674 3255 4168 7860 Size: 621 498 63 1492 581 913 3692 Fragments arranged by size: 3692 1492 913 621 581 498 63 PciI A'CATG_T Cuts at: 0 617 7860 Size: 617 7243 PflMI CCAn_nnn'nTGG Cuts at: 0 309 3063 4272 7860 Size: 309 2754 1209 3588 Fragments arranged by size: 3588 2754 1209 309 PfoI T'CCnGG_A Cuts at: 0 1025 7860 Size: 1025 6835 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 5531 5563 7860 Size: 5531 32 2297 Fragments arranged by size: 5531 2297 32 PpuMI rG'GwC_Cy Cuts at: 0 3113 5612 7860 Size: 3113 2499 2248 Fragments arranged by size: 3113 2499 2248 PshAI GACnn'nnGTC Cuts at: 0 3849 3891 4188 7478 7860 Size: 3849 42 297 3290 382 Fragments arranged by size: 3849 3290 382 297 42 PsiI TTA'TAA Cuts at: 0 4966 7860 Size: 4966 2894 PspOMI G'GGCC_C Cuts at: 0 193 204 7860 Size: 193 11 7656 Fragments arranged by size: 7656 193 11 PspXI vC'TCGA_Gb Cuts at: 0 3944 7860 Size: 3944 3916 PstI C_TGCA'G Cuts at: 0 1497 4029 5243 7536 7860 Size: 1497 2532 1214 2293 324 Fragments arranged by size: 2532 2293 1497 1214 324 PvuII CAG'CTG Cuts at: 0 2050 3565 4371 4461 4660 4840 5118 7860 Size: 2050 1515 806 90 199 180 278 2742 Fragments arranged by size: 2742 2050 1515 806 278 199 180 90 SacI G_AGCT'C Cuts at: 0 1091 2433 7860 Size: 1091 1342 5427 Fragments arranged by size: 5427 1342 1091 SacII CC_GC'GG Cuts at: 0 211 255 7860 Size: 211 44 7605 Fragments arranged by size: 7605 211 44 SanDI GG'GwC_CC Cuts at: 0 3113 7860 Size: 3113 4747 SapI GCTCTTCn'nnn_ Cuts at: 0 1546 2317 3272 7860 Size: 1546 771 955 4588 Fragments arranged by size: 4588 1546 955 771 ScaI AGT'ACT Cuts at: 0 5245 6850 7546 7860 Size: 5245 1605 696 314 Fragments arranged by size: 5245 1605 696 314 SfcI C'TryA_G Cuts at: 0 1277 1493 2544 2720 2810 3718 4025 4252 Size: 1277 216 1051 176 90 908 307 227 Cuts at: 4252 5239 7532 7591 7792 7860 Size: 987 2293 59 201 68 Fragments arranged by size: 2293 1277 1051 987 908 307 227 216 201 176 90 68 59 SfiI GGCCn_nnn'nGGCC Cuts at: 0 6255 7860 Size: 6255 1605 SmaI CCC'GGG Cuts at: 0 192 3500 7860 Size: 192 3308 4360 Fragments arranged by size: 4360 3308 192 SmlI C'TyrA_G Cuts at: 0 2073 2287 3944 5365 6373 7122 7860 Size: 2073 214 1657 1421 1008 749 738 Fragments arranged by size: 2073 1657 1421 1008 749 738 214 SpeI A'CTAG_T Cuts at: 0 2088 4480 5335 7860 Size: 2088 2392 855 2525 Fragments arranged by size: 2525 2392 2088 855 SphI G_CATG'C Cuts at: 0 1119 1182 4168 7860 Size: 1119 63 2986 3692 Fragments arranged by size: 3692 2986 1119 63 SspI AAT'ATT Cuts at: 0 6634 7376 7860 Size: 6634 742 484 Fragments arranged by size: 6634 742 484 StuI AGG'CCT Cuts at: 0 1714 1812 7860 Size: 1714 98 6048 Fragments arranged by size: 6048 1714 98 StyI C'CwwG_G Cuts at: 0 164 1715 2192 2489 3191 3283 5012 6948 Size: 164 1551 477 297 702 92 1729 1936 Cuts at: 6948 7860 Size: 912 Fragments arranged by size: 1936 1729 1551 912 702 477 297 164 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 676 1881 7119 7860 Size: 676 1205 5238 741 Fragments arranged by size: 5238 1205 741 676 TatI w'GTAC_w Cuts at: 0 4373 4885 5243 6848 6873 7259 7544 7860 Size: 4373 512 358 1605 25 386 285 316 Fragments arranged by size: 4373 1605 512 386 358 316 285 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 827 859 5160 5192 5899 5931 6308 6340 Size: 827 32 4301 32 707 32 377 32 Cuts at: 6340 7860 Size: 1520 Fragments arranged by size: 4301 1520 827 707 377 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 488 1006 7860 Size: 488 518 6854 Fragments arranged by size: 6854 518 488 XbaI T'CTAG_A Cuts at: 0 4810 5550 7860 Size: 4810 740 2310 Fragments arranged by size: 4810 2310 740 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 681 7860 Size: 681 7179 XhoI C'TCGA_G Cuts at: 0 3944 7860 Size: 3944 3916 XmaI C'CCGG_G Cuts at: 0 190 3498 7860 Size: 190 3308 4362 Fragments arranged by size: 4362 3308 190 XmnI GAAnn'nnTTC Cuts at: 0 2525 5272 7354 7677 7860 Size: 2525 2747 2082 323 183 Fragments arranged by size: 2747 2525 2082 323 183 ZraI GAC'GTC Cuts at: 0 6819 7860 Size: 6819 1041 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfiI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AgeI ApaLI AscI AsiSI BaeI BbeI BcgI BmgBI BmtI BsiWI BsmBI BspEI BssHII BstAPI BstEII BtgZI CspCI EcoRV FseI FspAI HpaI KasI MluI NarI NheI NotI NruI PacI PasI PmeI PmlI PsrI PvuI RsrII SalI SbfI SexAI SfoI SgfI SgrAI SnaBI SrfI SwaI