(Circular) (Six-base) MAPSORT of: pFN21AE3182.seq Check: 2060 from: 1 to: 5962 pFN21AE3182 5962 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2048 3305 1937 Size: 111 1257 4594 Fragments arranged by size: 4594 1257 111 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AfeI AGC'GCT Cuts at: 2372 2372 Size: 5962 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2043 3186 4861 5838 1203 Size: 840 1143 1675 977 1327 Fragments arranged by size: 1675 1327 1143 977 840 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 2662 4691 2662 Size: 2029 3933 AleI CACnn'nnGTG Cuts at: 1166 2612 1166 Size: 1446 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2729 1184 Size: 1545 4417 AlwNI CAG_nnn'CTG Cuts at: 2485 2749 3139 3705 5277 2485 Size: 264 390 566 1572 3170 Fragments arranged by size: 3170 1572 566 390 264 ApaLI G'TGCA_C Cuts at: 2990 4019 5175 2990 Size: 1029 1156 3777 Fragments arranged by size: 3777 1156 1029 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2293 2438 3287 1870 Size: 73 136 214 145 849 4545 Fragments arranged by size: 4545 849 214 145 136 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2123 3283 4738 618 Size: 325 217 627 288 48 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 325 288 217 48 BanII G_rGCy'C Cuts at: 1391 2097 2250 2445 3281 1391 Size: 706 153 195 836 4072 Fragments arranged by size: 4072 836 706 195 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2081 2115 4153 4187 1903 Size: 34 144 34 2038 34 3678 Fragments arranged by size: 3678 2038 144 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2628 3003 4573 Size: 107 122 71 631 605 956 375 1570 Cuts at: 4573 136 Size: 1525 Fragments arranged by size: 1570 1525 956 631 605 375 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3164 3379 3164 Size: 215 5747 Bme1580I G_kGCm'C Cuts at: 1790 2078 2994 4023 5179 1790 Size: 288 916 1029 1156 2573 Fragments arranged by size: 2573 1156 1029 916 288 BmgBI CAC'GTC Cuts at: 1318 2797 1318 Size: 1479 4483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2920 3693 4651 Size: 416 53 83 399 24 1503 773 958 Cuts at: 4651 442 Size: 1753 Fragments arranged by size: 1753 1503 958 773 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2929 3272 4622 1239 Size: 977 713 343 1350 2579 Fragments arranged by size: 2579 1350 977 713 343 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 4625 882 Size: 617 1059 2067 2219 Fragments arranged by size: 2219 2067 1059 617 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 2894 5956 2894 Size: 3062 2900 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 3769 4151 4786 Size: 53 83 186 936 1539 697 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1539 1451 936 697 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 1186 Size: 1018 4944 BseYI C'CCAG_C Cuts at: 2029 2069 2617 2681 3157 5165 5661 2029 Size: 40 548 64 476 2008 496 2330 Fragments arranged by size: 2330 2008 548 496 476 64 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2240 2427 3412 4173 5201 1540 Size: 275 186 239 187 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 239 187 186 BsiHKAI G_wGCw'C Cuts at: 2250 2466 2559 2802 2865 2994 4023 4108 5179 Size: 216 93 243 63 129 1029 85 1071 Cuts at: 5179 2250 Size: 3033 Fragments arranged by size: 3033 1071 1029 243 216 129 93 85 63 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 BsmBI CGTCTCn'nnnn_ Cuts at: 2264 2264 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2097 2250 2445 2466 2559 2802 Size: 399 288 19 153 195 21 93 243 Cuts at: 2802 2865 2994 3281 4023 4108 5179 1391 Size: 63 129 287 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 288 287 243 195 153 129 93 85 63 21 19 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2337 3317 844 Size: 1493 980 3489 Fragments arranged by size: 3489 1493 980 BsrBI CCG'CTC Cuts at: 1394 1823 2340 2967 3015 3151 3849 1394 Size: 429 517 627 48 136 698 3507 Fragments arranged by size: 3507 698 627 517 429 136 48 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 4606 4793 1700 Size: 110 425 2371 187 2869 Fragments arranged by size: 2869 2371 425 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 2201 1925 Size: 276 5686 BssSI C'ACGA_G Cuts at: 2243 4022 5034 2243 Size: 1779 1012 3171 Fragments arranged by size: 3171 1779 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3174 794 Size: 2380 3582 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2284 2791 2284 Size: 507 5455 BstYI r'GATC_y Cuts at: 1972 2745 2889 3292 3659 4044 4061 5513 5599 Size: 773 144 403 367 385 17 1452 86 Cuts at: 5599 5611 5957 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 773 403 385 367 346 144 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2937 2937 Size: 5962 BtgI C'CryG_G Cuts at: 513 1065 2020 2421 3258 513 Size: 552 955 401 837 3217 Fragments arranged by size: 3217 955 837 552 401 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2557 2692 2992 3478 4272 4292 2557 Size: 135 300 486 794 20 4227 Fragments arranged by size: 4227 794 486 300 135 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2959 2994 529 Size: 35 2395 35 3497 Fragments arranged by size: 3497 2395 35 35 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2274 2286 Size: 54 1425 50 275 18 407 37 12 Cuts at: 2286 2394 2424 3409 4298 5927 8 Size: 108 30 985 889 1629 43 Fragments arranged by size: 1629 1425 985 889 407 275 108 54 50 43 37 30 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2237 2424 3409 1537 Size: 275 425 187 985 4090 Fragments arranged by size: 4090 985 425 275 187 EarI CTCTTCn'nnn_ Cuts at: 1353 2255 2587 3892 1353 Size: 902 332 1305 3423 Fragments arranged by size: 3423 1305 902 332 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2248 2248 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2929 3272 4025 4622 5409 5677 Size: 393 584 713 343 753 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 787 753 713 597 584 393 343 268 EcoNI CCTnn'n_nnAGG Cuts at: 2318 2318 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2629 2629 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2389 2389 Size: 5962 FspI TGC'GCA Cuts at: 2318 2636 4468 2318 Size: 318 1832 3812 Fragments arranged by size: 3812 1832 318 HaeII r_GCGC'y Cuts at: 1678 2175 2374 2451 5109 1678 Size: 497 199 77 2658 2531 Fragments arranged by size: 2658 2531 497 199 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2648 2680 4603 4635 4677 Size: 32 135 32 1076 32 1923 32 42 Cuts at: 4677 4709 1373 Size: 32 2626 Fragments arranged by size: 2626 1923 1076 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2049 3306 3552 669 Size: 988 281 111 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 3186 5838 3186 Size: 2652 3310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 2679 3126 5076 5260 1305 Size: 1341 33 447 1950 184 2007 Fragments arranged by size: 2007 1950 1341 447 184 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 10 Size: 54 1425 343 456 3684 Fragments arranged by size: 3684 1425 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 3920 4279 4438 518 Size: 648 1446 1308 359 159 2042 Fragments arranged by size: 2042 1446 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 3136 3378 4055 5203 5448 1606 Size: 416 1114 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 1114 677 416 245 242 NaeI GCC'GGC Cuts at: 2237 2237 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 2235 2235 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2047 3320 4865 1132 Size: 97 818 1273 1545 2229 Fragments arranged by size: 2229 1545 1273 818 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 2043 4861 2043 Size: 2818 3144 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2187 3314 830 Size: 1357 1127 3478 Fragments arranged by size: 3478 1357 1127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 3136 3136 Size: 5962 RsrII CG'GwC_CG Cuts at: 2668 2668 Size: 5962 SacI G_AGCT'C Cuts at: 2250 2250 Size: 5962 SacII CC_GC'GG Cuts at: 2023 2023 Size: 5962 SalI G'TCGA_C Cuts at: 1936 2047 3304 1936 Size: 111 1257 4594 Fragments arranged by size: 4594 1257 111 SapI GCTCTTCn'nnn_ Cuts at: 2255 2587 2255 Size: 332 5630 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 2549 4210 5922 2549 Size: 1661 1712 2589 Fragments arranged by size: 2589 1712 1661 SfcI C'TryA_G Cuts at: 826 1045 2183 2477 3310 4445 5126 5317 826 Size: 219 1138 294 833 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 833 681 294 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2628 2628 Size: 5962 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 2081 2295 3289 1872 Size: 209 214 994 4545 Fragments arranged by size: 4545 994 214 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2507 1517 Size: 990 4972 StyI C'CwwG_G Cuts at: 513 1065 2518 2775 3401 513 Size: 552 1453 257 626 3074 Fragments arranged by size: 3074 1453 626 552 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2620 2685 2854 3989 4006 4159 4344 Size: 1255 1040 65 169 1135 17 153 185 Cuts at: 4344 4789 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1135 1040 445 185 169 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 4208 5920 Size: 274 80 33 51 1443 570 1661 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1661 1443 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 3083 1316 Size: 1767 4195 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2614 2614 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 2079 2293 3287 1870 Size: 209 214 994 4545 Fragments arranged by size: 4545 994 214 209 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SanDI SexAI SfoI SrfI SwaI