Restriction map for
pFN21AE3154
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I ClaI
DpnII DraI DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI MamI
MboI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NdeII
NheI NruI NsiI NspI NspV
PacI PaeR7I PinAI PleI PmeI
PshBI Psp1406I PstI PvuI PvuII
RcaI SacI SalI Sau3AI SbfI
ScaI SfaNI SfcI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 240 GACGTC GACGT^C
AfaI 816 GTAC GT^AC
AspHI 1039 GTGCTC G[AT]GC[AT]^C
AspI 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 875 TGGCCA TGG^CCA
BbrPI 271 CACGTG CAC^GTG
BbsI 388 GTCTTC GAAGAC(2/6)
BfaI 127 CTAG C^TAG
BpuAI 388 GTCTTC GAAGAC(2/6)
BsaI 217 GAGACC GGTCTC(1/5)
BsiHKAI 1039 GTGCTC G[AT]GC[AT]^C
BsmAI 217 GAGAC GTCTC(1/5)
BspMI 998 ACCTGC ACCTGC(4/8)
BsrDI 367 CATTGC GCAATG(2/0)
BstF5I 312 CATCC GGATG(2/0)
CpoI 317 CGGACCG CG^G[AT]CCG
DraIII 949 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1042 CTCTTC CTCTTC(1/4)
FokI 312 CATCC GGATG(9/13)
Ksp632I 1042 CTCTTC CTCTTC(1/4)
MaeI 127 CTAG C^TAG
MluNI 875 TGGCCA TGG^CCA
MscI 875 TGGCCA TGG^CCA
NaeI 936 GCCGGC GCC^GGC
NgoMIV 936 GCCGGC G^CCGGC
NotI 976 GCGGCCGC GC^GGCCGC
PflFI 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 735 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 271 CACGTG CAC^GTG
PmlI 271 CACGTG CAC^GTG
PshAI 381 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 816 GTAC GT^AC
RsrII 317 CGGACCG CG^G[AT]CCG
SapI 1041 GCTCTTC GCTCTTC(1/4)
SexAI 220 ACCAGGT A^CC[AT]GGT
SgrAI 935 CGCCGGCG C[GA]^CCGG[CT]G
StuI 695 AGGCCT AGG^CCT
Tth111I 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 735 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 431 GGGCCC GGGCC^C
665 GGGCCC
BbeI 188 GGCGCC GGCGC^C
598 GGCGCC
BglI 601 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
785 GCCCCGGGGGC
BlpI 415 GCTGAGC GC^T[ACGT]AGC
492 GCTGAGC
BpmI 118 CTCCAG CTGGAG(16/14)
439 CTGGAG
Bpu1102I 415 GCTGAGC GC^T[ACGT]AGC
492 GCTGAGC
BsaAI 271 CACGTG [CT]AC^GT[GA]
817 TACGTG
BsrI 552 CCAGT ACTGG(1/-1)
577 CCAGT
BstEII 252 GGTCACC G^GT[ACGT]ACC
711 GGTGACC
BstPI 252 GGTCACC G^GT[ACGT]ACC
711 GGTGACC
CelII 415 GCTGAGC GC^T[ACGT]AGC
492 GCTGAGC
EcoNI 114 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
687 CCTGGCCGAGG
EcoO65I 252 GGTCACC G^GT[ACGT]ACC
711 GGTGACC
HgaI 200 GACGC GACGC(5/10)
760 GACGC
HphI 711 GGTGA GGTGA(8/7)
254 TCACC
KasI 188 GGCGCC G^GCGCC
598 GGCGCC
MaeIII 253 GTCAC ^GT[ACGT]AC
712 GTGAC
MboII 389 TCTTC GAAGA(8/7)
1043 TCTTC
MslI 1032 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1089 CATGGTGGTG
NarI 188 GGCGCC GG^CGCC
598 GGCGCC
PpuMI 18 AGGTCCC [GA]G^G[AT]CC[CT]
206 GGGACCC
SfoI 188 GGCGCC GGC^GCC
598 GGCGCC
Tsp45I 253 GTCAC ^GT[GC]AC
712 GTGAC
TspRI 578 CAGTG CA[GC]TG[ACGT][ACGT]^
895 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KspI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV SacII
Sau96I ScrFI SmaI TaqI TseI
TthHB8I XmaCI XmaI